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The map label for this gene is ackA [H]

Identifier: 71893856

GI number: 71893856

Start: 651263

End: 652471

Strand: Reverse

Name: ackA [H]

Synonym: MHJ_0505

Alternate gene names: 71893856

Gene position: 652471-651263 (Counterclockwise)

Preceding gene: 71893857

Following gene: 71893853

Centisome position: 72.71

GC content: 35.15

Gene sequence:

>1209_bases
GTGAAAGCAAAAATTTTAGTAGTAAATGCTGGTTCTTCATCAATTAAATGGCAGATTTTCCAAAAGGAAAATCTTGAATT
ATTAGGTGTAGGCCTAATTGAAAGAATTGGTCTTGAAGATGGTCTAATCAAAATGTCTTTTGGCAAAAAAGACTATAAAT
ACAAAGATAATTTTGCAAATCACAGTCAGGCACTAGCAGTCCAAATTGCCTTTTGGAAGGAAAATCAGCTTGTTGAAAAT
CTTGAAGAATTCCAACTTGTTGGATTTAGAATTGTCCATGGTGGGGCAAGTTTTAAAGCTCCGGCAAAATTAGATCAGAT
GGCAATTTCCAAAATTGAAGCAGCTGCAAAATTTGCGCCATTGCACAATCCCGGCGCCCTGGCAACAATTAAAGCAGTTA
AAGAATTAATTCCAAATGCAAAACTGGCTGCAAGTTTTGATACCGCCTTTCATGGTACTATCCCAAAAATCAATTATACC
TACCCAATTAATGCCGAAATTGCTGATAAATATGGAATTAGAAAGTACGGTTTTCACGGGATTTCTCATAAATTTATTAC
CACACAAGTTGAAAAAATTTATGACTCTGACAAGGTAAATTTTGTCAATATGCATATTGGTAATGGAATTTCACTCTGCG
CAATTAAAAATTCACAGTCGTTAGATACTTCAATGGGAATGACACCGCTAGCCGGGGTTGCCATGGGGACTCGAAGCGGT
GATATTGATCCATCAATTATACCATATTTGGCAAATGAGGCAAGTTTTTCTTTTAGCGAAATTGATCATTTACTAAATAA
AAATTCCGGACTTTTAGGGCTTTCACGGGTATCTTCTGATCTTCGCGATGTAATTTCAGCAAAAAATAGTGGTAATTTAG
AGGCAAGTTTTGCCCTTGAAGTTTATGTCCAAAAAATTGTTGATTATCTTGTAAATTATATAAATAAGGTCGGTAAAAAC
ATTAAGGCGCTTGTTTTTACAGGTGGTGTTGGCGAAAATTCAGCTTTTATTCGGTCCCTGGTAATCGAAAAAGTTCAAAT
ACCCGGCTTAAATTTAGTTCTTGACCAAGAAAAAAACTTAAAACCAATCCCAGAATTTGGACCAATTGAGAAAATTTCAG
CTCCTGAATCCGATCTTGATATTTTTGTAATTAAAACTAACGAAGAGCTTTTAATTGCCAAAAATGCGATCAGCGTTTGG
GAAAATTAA

Upstream 100 bases:

>100_bases
GAATTAATGCCCCAATTAATGATTTATCCCGGGGATCAACAACTCAAGATGTCTTTAATACCGTGCTAATTAGCGCTCTA
CAAGTTGAAGAAGGTAAATA

Downstream 100 bases:

>100_bases
ATTTAATTTATTTTATATTATACAACAAAAAATTAGGTTTTTATTAAAAAAAACAAAAACCTAATTTTTTTTAAGTTAGT
ATATTAATTTATAGTGTTTT

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKAKILVVNAGSSSIKWQIFQKENLELLGVGLIERIGLEDGLIKMSFGKKDYKYKDNFANHSQALAVQIAFWKENQLVEN
LEEFQLVGFRIVHGGASFKAPAKLDQMAISKIEAAAKFAPLHNPGALATIKAVKELIPNAKLAASFDTAFHGTIPKINYT
YPINAEIADKYGIRKYGFHGISHKFITTQVEKIYDSDKVNFVNMHIGNGISLCAIKNSQSLDTSMGMTPLAGVAMGTRSG
DIDPSIIPYLANEASFSFSEIDHLLNKNSGLLGLSRVSSDLRDVISAKNSGNLEASFALEVYVQKIVDYLVNYINKVGKN
IKALVFTGGVGENSAFIRSLVIEKVQIPGLNLVLDQEKNLKPIPEFGPIEKISAPESDLDIFVIKTNEELLIAKNAISVW
EN

Sequences:

>Translated_402_residues
MKAKILVVNAGSSSIKWQIFQKENLELLGVGLIERIGLEDGLIKMSFGKKDYKYKDNFANHSQALAVQIAFWKENQLVEN
LEEFQLVGFRIVHGGASFKAPAKLDQMAISKIEAAAKFAPLHNPGALATIKAVKELIPNAKLAASFDTAFHGTIPKINYT
YPINAEIADKYGIRKYGFHGISHKFITTQVEKIYDSDKVNFVNMHIGNGISLCAIKNSQSLDTSMGMTPLAGVAMGTRSG
DIDPSIIPYLANEASFSFSEIDHLLNKNSGLLGLSRVSSDLRDVISAKNSGNLEASFALEVYVQKIVDYLVNYINKVGKN
IKALVFTGGVGENSAFIRSLVIEKVQIPGLNLVLDQEKNLKPIPEFGPIEKISAPESDLDIFVIKTNEELLIAKNAISVW
EN
>Mature_402_residues
MKAKILVVNAGSSSIKWQIFQKENLELLGVGLIERIGLEDGLIKMSFGKKDYKYKDNFANHSQALAVQIAFWKENQLVEN
LEEFQLVGFRIVHGGASFKAPAKLDQMAISKIEAAAKFAPLHNPGALATIKAVKELIPNAKLAASFDTAFHGTIPKINYT
YPINAEIADKYGIRKYGFHGISHKFITTQVEKIYDSDKVNFVNMHIGNGISLCAIKNSQSLDTSMGMTPLAGVAMGTRSG
DIDPSIIPYLANEASFSFSEIDHLLNKNSGLLGLSRVSSDLRDVISAKNSGNLEASFALEVYVQKIVDYLVNYINKVGKN
IKALVFTGGVGENSAFIRSLVIEKVQIPGLNLVLDQEKNLKPIPEFGPIEKISAPESDLDIFVIKTNEELLIAKNAISVW
EN

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=399, Percent_Identity=39.0977443609023, Blast_Score=251, Evalue=8e-68,
Organism=Escherichia coli, GI145693184, Length=397, Percent_Identity=36.272040302267, Blast_Score=251, Evalue=8e-68,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 44075; Mature: 44075

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS01075 ACETATE_KINASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKILVVNAGSSSIKWQIFQKENLELLGVGLIERIGLEDGLIKMSFGKKDYKYKDNFAN
CCEEEEEEECCCCCEEEEEEECCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCC
HSQALAVQIAFWKENQLVENLEEFQLVGFRIVHGGASFKAPAKLDQMAISKIEAAAKFAP
CCCEEEEEEEEECCCHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
LHNPGALATIKAVKELIPNAKLAASFDTAFHGTIPKINYTYPINAEIADKYGIRKYGFHG
CCCCCCHHHHHHHHHHCCCCEEEEECCCHHCCCCCCEEEEEECCCCHHHHCCCHHHCCCC
ISHKFITTQVEKIYDSDKVNFVNMHIGNGISLCAIKNSQSLDTSMGMTPLAGVAMGTRSG
CCCHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCHHCCCCCHHHEEECCCCC
DIDPSIIPYLANEASFSFSEIDHLLNKNSGLLGLSRVSSDLRDVISAKNSGNLEASFALE
CCCCCHHHHCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEHHHH
VYVQKIVDYLVNYINKVGKNIKALVFTGGVGENSAFIRSLVIEKVQIPGLNLVLDQEKNL
HHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCC
KPIPEFGPIEKISAPESDLDIFVIKTNEELLIAKNAISVWEN
CCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHCCC
>Mature Secondary Structure
MKAKILVVNAGSSSIKWQIFQKENLELLGVGLIERIGLEDGLIKMSFGKKDYKYKDNFAN
CCEEEEEEECCCCCEEEEEEECCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCC
HSQALAVQIAFWKENQLVENLEEFQLVGFRIVHGGASFKAPAKLDQMAISKIEAAAKFAP
CCCEEEEEEEEECCCHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
LHNPGALATIKAVKELIPNAKLAASFDTAFHGTIPKINYTYPINAEIADKYGIRKYGFHG
CCCCCCHHHHHHHHHHCCCCEEEEECCCHHCCCCCCEEEEEECCCCHHHHCCCHHHCCCC
ISHKFITTQVEKIYDSDKVNFVNMHIGNGISLCAIKNSQSLDTSMGMTPLAGVAMGTRSG
CCCHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCHHCCCCCHHHEEECCCCC
DIDPSIIPYLANEASFSFSEIDHLLNKNSGLLGLSRVSSDLRDVISAKNSGNLEASFALE
CCCCCHHHHCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEHHHH
VYVQKIVDYLVNYINKVGKNIKALVFTGGVGENSAFIRSLVIEKVQIPGLNLVLDQEKNL
HHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCC
KPIPEFGPIEKISAPESDLDIFVIKTNEELLIAKNAISVWEN
CCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11353084 [H]