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The map label for this gene is asnS [H]

Identifier: 71893848

GI number: 71893848

Start: 637586

End: 638548

Strand: Reverse

Name: asnS [H]

Synonym: MHJ_0497

Alternate gene names: 71893848

Gene position: 638548-637586 (Counterclockwise)

Preceding gene: 71893849

Following gene: 71893847

Centisome position: 71.15

GC content: 26.06

Gene sequence:

>963_bases
ATATCAAGTGAAAGTTATGCCAAAATATTAACAATTTATTCAGAAATTAATTTTTTAACATACAAGTGAATGCGCGATAA
CAATGTAAAAATAGTAAATTGTCCAATAACAACACAAAGTATTTCAAGTCCTATGGGGCTCGGAAGTGATTCACTACCTT
ATAAAGTTATTTCGAAAAATAATCCGAATTTTGAGTTTTATTTAGCAGACAGCATGCAATTTCATTTGGAATTATTTTTA
AGAACAAAAAATGTTGAATCAGTCGGATATATTGCCCCAACATTTCGTGGCGAAAATGTTGATGAAAGACATTTACATCA
ATTTAATCATTTTGAGATTGAAACAAAAAAACCATTAGTTGAAACAAAAAAAATGATTATAAATTATCTAAAATATTTAG
TTAAAAATTTAGTTAAAACACATTCCGCTGAACTAAATTATTTTAATGAAAATAATGTACCAAGACTTTTGAAGTGACTA
AACTTTGATCAATATAATTTATTATTTACAAATGCTATAAAAATATTAGATGAAAAATTTAAAGAAGGCATCGATCGAAC
CGAACTACTTTTAAATATAAATTCAAAAGGTGAAAAATTTTTAATTAATTATTTTTCTGATAAAATTTTATGGTTAGATA
ATTTTCCAGAAATTAATGTACCTTTTTATCAAAAAAGTAAAGATGGTTTTGCGATTAATTCAGATTTATTAATGGGAATC
GGAGAAATAGTTGGAAGTGGTGAAAGGTGTTTTGATTATAATGAAACATTAAAATCCCTTTTAAACCACAAAAATAACCC
ACAAAATTATAAATGATATTTAGAAATGAAAGAAAAAACTCCGATGATTACTTCTGGATTTGGTTTAGGAATGGAAAGAT
TGATGCTATTTTTAATTGGAAAAAATGATATACGCGAAGTTCAACTAATCCCGAGAGAAACTGACAAGGAAATTCTCCCC
TAA

Upstream 100 bases:

>100_bases
AAAAATTTTGTTAGAATTTCCTTACTACGCAAAAAAATATAAAAAAGGAAACTCAATAACTGTGAATTTTAAGAATTTTA
AGCATGATTTTATAAAGCAC

Downstream 100 bases:

>100_bases
AATGACAGAAACGATCAAAATAATTGCTGCATATTTGCAGGAATTTAACAAAAAAGAAGGTCTAAATTTAAGAATTTTCC
TCAAAGGAAGTTATATTCAC

Product: asparagine synthetase A

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSSESYAKILTIYSEINFLTYKWMRDNNVKIVNCPITTQSISSPMGLGSDSLPYKVISKNNPNFEFYLADSMQFHLELFL
RTKNVESVGYIAPTFRGENVDERHLHQFNHFEIETKKPLVETKKMIINYLKYLVKNLVKTHSAELNYFNENNVPRLLKWL
NFDQYNLLFTNAIKILDEKFKEGIDRTELLLNINSKGEKFLINYFSDKILWLDNFPEINVPFYQKSKDGFAINSDLLMGI
GEIVGSGERCFDYNETLKSLLNHKNNPQNYKWYLEMKEKTPMITSGFGLGMERLMLFLIGKNDIREVQLIPRETDKEILP

Sequences:

>Translated_320_residues
MSSESYAKILTIYSEINFLTYK*MRDNNVKIVNCPITTQSISSPMGLGSDSLPYKVISKNNPNFEFYLADSMQFHLELFL
RTKNVESVGYIAPTFRGENVDERHLHQFNHFEIETKKPLVETKKMIINYLKYLVKNLVKTHSAELNYFNENNVPRLLK*L
NFDQYNLLFTNAIKILDEKFKEGIDRTELLLNINSKGEKFLINYFSDKILWLDNFPEINVPFYQKSKDGFAINSDLLMGI
GEIVGSGERCFDYNETLKSLLNHKNNPQNYK*YLEMKEKTPMITSGFGLGMERLMLFLIGKNDIREVQLIPRETDKEILP
>Mature_319_residues
SSESYAKILTIYSEINFLTYK*MRDNNVKIVNCPITTQSISSPMGLGSDSLPYKVISKNNPNFEFYLADSMQFHLELFLR
TKNVESVGYIAPTFRGENVDERHLHQFNHFEIETKKPLVETKKMIINYLKYLVKNLVKTHSAELNYFNENNVPRLLK*LN
FDQYNLLFTNAIKILDEKFKEGIDRTELLLNINSKGEKFLINYFSDKILWLDNFPEINVPFYQKSKDGFAINSDLLMGIG
EIVGSGERCFDYNETLKSLLNHKNNPQNYK*YLEMKEKTPMITSGFGLGMERLMLFLIGKNDIREVQLIPRETDKEILP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI226491502, Length=325, Percent_Identity=25.8461538461538, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1787161, Length=232, Percent_Identity=26.7241379310345, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319872, Length=330, Percent_Identity=25.4545454545455, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921528, Length=258, Percent_Identity=27.5193798449612, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19922416, Length=257, Percent_Identity=29.5719844357977, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.22 [H]

Molecular weight: Translated: 36898; Mature: 36767

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSESYAKILTIYSEINFLTYKMRDNNVKIVNCPITTQSISSPMGLGSDSLPYKVISKNN
CCCCHHHHHHHHEECCEEEEEEEECCCEEEEECCCCHHHCCCCCCCCCCCCCEEEEECCC
PNFEFYLADSMQFHLELFLRTKNVESVGYIAPTFRGENVDERHLHQFNHFEIETKKPLVE
CCEEEEEECCCEEEEEEEEEECCCCCCCEEECCCCCCCCCHHHHHHCCCEEEECCCCHHH
TKKMIINYLKYLVKNLVKTHSAELNYFNENNVPRLLKLNFDQYNLLFTNAIKILDEKFKE
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHC
GIDRTELLLNINSKGEKFLINYFSDKILWLDNFPEINVPFYQKSKDGFAINSDLLMGIGE
CCCCEEEEEEECCCCCEEEEEECCCEEEEECCCCCCCCCEEECCCCCEEECHHHHHHHHH
IVGSGERCFDYNETLKSLLNHKNNPQNYKYLEMKEKTPMITSGFGLGMERLMLFLIGKND
HHCCCCCEECHHHHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCCCHHHHHEEEECCCC
IREVQLIPRETDKEILP
CEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SSESYAKILTIYSEINFLTYKMRDNNVKIVNCPITTQSISSPMGLGSDSLPYKVISKNN
CCCHHHHHHHHEECCEEEEEEEECCCEEEEECCCCHHHCCCCCCCCCCCCCEEEEECCC
PNFEFYLADSMQFHLELFLRTKNVESVGYIAPTFRGENVDERHLHQFNHFEIETKKPLVE
CCEEEEEECCCEEEEEEEEEECCCCCCCEEECCCCCCCCCHHHHHHCCCEEEECCCCHHH
TKKMIINYLKYLVKNLVKTHSAELNYFNENNVPRLLKLNFDQYNLLFTNAIKILDEKFKE
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHC
GIDRTELLLNINSKGEKFLINYFSDKILWLDNFPEINVPFYQKSKDGFAINSDLLMGIGE
CCCCEEEEEEECCCCCEEEEEECCCEEEEECCCCCCCCCEEECCCCCEEECHHHHHHHHH
IVGSGERCFDYNETLKSLLNHKNNPQNYKYLEMKEKTPMITSGFGLGMERLMLFLIGKND
HHCCCCCEECHHHHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCCCHHHHHEEEECCCC
IREVQLIPRETDKEILP
CEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA