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The map label for this gene is trmB [H]

Identifier: 71893813

GI number: 71893813

Start: 586199

End: 586804

Strand: Reverse

Name: trmB [H]

Synonym: MHJ_0462

Alternate gene names: 71893813

Gene position: 586804-586199 (Counterclockwise)

Preceding gene: 71893814

Following gene: 71893812

Centisome position: 65.39

GC content: 26.73

Gene sequence:

>606_bases
ATGCGTCTAAGAAATATTCCTGATGCGCTTGAAAGAATTCAAAATCAGAATTTACTTGTAAAAACACCCTGAAATATTGA
TGATTCTTGAATAATAGAGATTGGAATGGGTAAGGGTAAAATGATAAGTCAACTTGCCTTTGATAATCCCAATAAAAATT
TTTTAGGCGTTGAAAAGTATCCTTCTGCCGCAGTTAAAGCAATAAAATATGTAAAAAAATATAATTTAAGTAATTTTTTT
ATCCTCATAAGTGATGCTAAAGATTTATTAGACCGGATAAAAGGAAAGGCAAGCACAATTTGGCTTACTTTTCCTGATCC
ATGACCAAAAAATCGTCATTACAAACGCCGCTTGACTTACAAAGATTTTTTGGAAATTTATGCTAATTTATTAGTAAAAG
ATGGTATTTTAAAATTAAAAACTGATAATTTAAAATTTTTTGAATTCTCAATTGAATCTTTAAAAGAAAATGGTTGAAAA
ATCACTTATCAAACAAATGATTTGCATAATTCTTTAGTAAATAGCAGCAATATCAAAACAACTTATGAGGAAAAATGAGT
TAATTTAAATTATAAAATCCATTATCTTGAAGCTATTTTTATCTAA

Upstream 100 bases:

>100_bases
TCGAAGAAATCGAAAGCGGAATTGTTGATGAACATTTTGAATTGCGTGGTCAACACGATTTAAGTTGTCATGCATAGTTT
AAAATATAAAATTTTTTAAT

Downstream 100 bases:

>100_bases
ACCTATATTTTTAAATATAGGTTTAACTTTTTTATTAGAAAAAATATTTTAAAAAAATATTTCTAATTTTTTAACTTTTT
TGTGTTATAATTTTATTTAC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MRLRNIPDALERIQNQNLLVKTPWNIDDSWIIEIGMGKGKMISQLAFDNPNKNFLGVEKYPSAAVKAIKYVKKYNLSNFF
ILISDAKDLLDRIKGKASTIWLTFPDPWPKNRHYKRRLTYKDFLEIYANLLVKDGILKLKTDNLKFFEFSIESLKENGWK
ITYQTNDLHNSLVNSSNIKTTYEEKWVNLNYKIHYLEAIFI

Sequences:

>Translated_201_residues
MRLRNIPDALERIQNQNLLVKTP*NIDDS*IIEIGMGKGKMISQLAFDNPNKNFLGVEKYPSAAVKAIKYVKKYNLSNFF
ILISDAKDLLDRIKGKASTIWLTFPDP*PKNRHYKRRLTYKDFLEIYANLLVKDGILKLKTDNLKFFEFSIESLKENG*K
ITYQTNDLHNSLVNSSNIKTTYEEK*VNLNYKIHYLEAIFI
>Mature_201_residues
MRLRNIPDALERIQNQNLLVKTP*NIDDS*IIEIGMGKGKMISQLAFDNPNKNFLGVEKYPSAAVKAIKYVKKYNLSNFF
ILISDAKDLLDRIKGKASTIWLTFPDP*PKNRHYKRRLTYKDFLEIYANLLVKDGILKLKTDNLKFFEFSIESLKENG*K
ITYQTNDLHNSLVNSSNIKTTYEEK*VNLNYKIHYLEAIFI

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=125, Percent_Identity=30.4, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 22692; Mature: 22692

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRNIPDALERIQNQNLLVKTPNIDDSIIEIGMGKGKMISQLAFDNPNKNFLGVEKYPS
CCCCCHHHHHHHHCCCCEEEECCCCCCHHEEECCCCCHHHHHHHCCCCCCCEECCCCCCH
AAVKAIKYVKKYNLSNFFILISDAKDLLDRIKGKASTIWLTFPDPPKNRHYKRRLTYKDF
HHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCHHHH
LEIYANLLVKDGILKLKTDNLKFFEFSIESLKENGKITYQTNDLHNSLVNSSNIKTTYEE
HHHHHHHHHHCCEEEEEECCEEEEEEEHHHHHCCCCEEEEECHHHHHHCCCCCCEEEEHH
KVNLNYKIHYLEAIFI
HCCCEEEEEEEEEEEC
>Mature Secondary Structure
MRLRNIPDALERIQNQNLLVKTPNIDDSIIEIGMGKGKMISQLAFDNPNKNFLGVEKYPS
CCCCCHHHHHHHHCCCCEEEECCCCCCHHEEECCCCCHHHHHHHCCCCCCCEECCCCCCH
AAVKAIKYVKKYNLSNFFILISDAKDLLDRIKGKASTIWLTFPDPPKNRHYKRRLTYKDF
HHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCHHHH
LEIYANLLVKDGILKLKTDNLKFFEFSIESLKENGKITYQTNDLHNSLVNSSNIKTTYEE
HHHHHHHHHHCCEEEEEECCEEEEEEEHHHHHCCCCEEEEECHHHHHHCCCCCCEEEEHH
KVNLNYKIHYLEAIFI
HCCCEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA