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The map label for this gene is ulaD [H]

Identifier: 71893787

GI number: 71893787

Start: 529953

End: 530621

Strand: Reverse

Name: ulaD [H]

Synonym: MHJ_0436

Alternate gene names: 71893787

Gene position: 530621-529953 (Counterclockwise)

Preceding gene: 71893788

Following gene: 71893786

Centisome position: 59.13

GC content: 37.37

Gene sequence:

>669_bases
ATGGCAGTCCCGCTCTTGCAAATCGCCCTTGACAATCAGAGTATTCAGGAGGCAATCGAGTCGGCAAAAAAGGCAGAAAA
GTACATTGATATTATCGAAGTTGGAACAATTTTGTTAGCTTCGGAAGGTAAAAAAGCAATTTCGGAATTAAGAAAAGCAT
TTCCTGATAAAATCATAGTAGCAGATGGTAAAATTGCGGATGCCGGAAATATTTTCGGGAAAATGTTTTTCGAAGCAGGC
GCTGATTTTACCACTGTAATTTGTGCAGCCGAAACAGCGACAATTAAGGATTTAGTTGATCTTTCAAAAAAATATTACCA
AAAAAATGGCAAAACCACTGAAATTCAAGTCGAAATGACAAATAATTTTGCTTGAGATCAAGTATCTGACTGAAAAGAAG
TAGGAGTTCCGCAAGTAGTCTGACACAGACCACGTGATTCGCAAGCTTCCGGGGTAAAATGAGCCCAAAAAGATATTGAT
ATTGTCAAAAAACTTGCTAATTTAGGTTTTAAGGTTACAATCACCGGCGGGGTTGAAGTTGAGGATATTAAATTATTTAA
GGATATCCCAATTTATATTTTTATCGCCGGCCGTTCAATTCGTGATGCAAAAGATCCAGAGGCAAAAGCAAAGGAATTCA
AGGATGAGTTTAAAAAGTACTGAACCTAA

Upstream 100 bases:

>100_bases
AAATTTCCGAATTTTTTTGAATTTTTTTCTTGGAAAAATTCAATTTTAGTGTATAATTACTATCACAAAAACCAACAAAA
AAATCGTGAAAAGGAGAGAA

Downstream 100 bases:

>100_bases
TTTTTTTGTCGGTATCTACGAAAAAGCAATTGATAAACGTTTTTCGTTAATCGATAAAATTAGAATCGCCAAAGAAGCTG
GTTTTAATTTTATCGAAATG

Product: 3-keto-L-gulonate-6-phosphate decarboxylase

Products: NA

Alternate protein names: KGPDC; 3-dehydro-L-gulonate-6-phosphate decarboxylase; L-ascorbate utilization protein D [H]

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MAVPLLQIALDNQSIQEAIESAKKAEKYIDIIEVGTILLASEGKKAISELRKAFPDKIIVADGKIADAGNIFGKMFFEAG
ADFTTVICAAETATIKDLVDLSKKYYQKNGKTTEIQVEMTNNFAWDQVSDWKEVGVPQVVWHRPRDSQASGVKWAQKDID
IVKKLANLGFKVTITGGVEVEDIKLFKDIPIYIFIAGRSIRDAKDPEAKAKEFKDEFKKYWT

Sequences:

>Translated_222_residues
MAVPLLQIALDNQSIQEAIESAKKAEKYIDIIEVGTILLASEGKKAISELRKAFPDKIIVADGKIADAGNIFGKMFFEAG
ADFTTVICAAETATIKDLVDLSKKYYQKNGKTTEIQVEMTNNFA*DQVSD*KEVGVPQVV*HRPRDSQASGVK*AQKDID
IVKKLANLGFKVTITGGVEVEDIKLFKDIPIYIFIAGRSIRDAKDPEAKAKEFKDEFKKY*T
>Mature_221_residues
AVPLLQIALDNQSIQEAIESAKKAEKYIDIIEVGTILLASEGKKAISELRKAFPDKIIVADGKIADAGNIFGKMFFEAGA
DFTTVICAAETATIKDLVDLSKKYYQKNGKTTEIQVEMTNNFA*DQVSD*KEVGVPQVV*HRPRDSQASGVK*AQKDIDI
VKKLANLGFKVTITGGVEVEDIKLFKDIPIYIFIAGRSIRDAKDPEAKAKEFKDEFKKY*T

Specific function: Catalyzes the decarboxylation of 3-keto-L-gulonate-6-P into L-xylulose-5-P. Is involved in the anaerobic L-ascorbate utilization [H]

COG id: COG0269

COG function: function code G; 3-hexulose-6-phosphate synthase and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPS/KGPDC family. KGPDC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790640, Length=214, Percent_Identity=47.6635514018692, Blast_Score=206, Evalue=9e-55,
Organism=Escherichia coli, GI1790006, Length=214, Percent_Identity=41.588785046729, Blast_Score=157, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.85 [H]

Molecular weight: Translated: 23815; Mature: 23683

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVPLLQIALDNQSIQEAIESAKKAEKYIDIIEVGTILLASEGKKAISELRKAFPDKIIV
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHCCCEEEE
ADGKIADAGNIFGKMFFEAGADFTTVICAAETATIKDLVDLSKKYYQKNGKTTEIQVEMT
ECCCCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
NNFADQVSDKEVGVPQVVHRPRDSQASGVKAQKDIDIVKKLANLGFKVTITGGVEVEDIK
CCHHHHCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCEEHHHH
LFKDIPIYIFIAGRSIRDAKDPEAKAKEFKDEFKKYT
HCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVPLLQIALDNQSIQEAIESAKKAEKYIDIIEVGTILLASEGKKAISELRKAFPDKIIV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHCCCEEEE
ADGKIADAGNIFGKMFFEAGADFTTVICAAETATIKDLVDLSKKYYQKNGKTTEIQVEMT
ECCCCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
NNFADQVSDKEVGVPQVVHRPRDSQASGVKAQKDIDIVKKLANLGFKVTITGGVEVEDIK
CCHHHHCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCEEHHHH
LFKDIPIYIFIAGRSIRDAKDPEAKAKEFKDEFKKYT
HCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633; 9274005 [H]