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The map label for this gene is araD [H]

Identifier: 71893785

GI number: 71893785

Start: 528376

End: 529098

Strand: Reverse

Name: araD [H]

Synonym: MHJ_0434

Alternate gene names: 71893785

Gene position: 529098-528376 (Counterclockwise)

Preceding gene: 71893786

Following gene: 71893784

Centisome position: 58.96

GC content: 37.34

Gene sequence:

>723_bases
ATGAAAATTAAAGACCGTAACGAACTTGAAGAACTCCAAAAAGCTGTCTTAGAAGCAAATCTTTTACTTTATAGGTCCAA
ATTAGCACTTCATACCTGGGGAAATGTCTCAGCAATTAGCAAAGATCGCAGTTATTATGTTATAAAACCCAGCGGAATTC
CTTATGAAAAAATGCAGTTTTCAGATATGGTTCCTGTTGATCTTGAAAATAATGTCCTTGAAACTAATCTAAACCCTTCA
AGTGATACTCCCACGCATTCACTGATCTACAAAGCCGATTCACGAATTCAAGCCATCGTCCATACCCATTCGCCCTTTGC
AGTGGCTTGAGCCCAAGCCGGAAAAGACATTCCAGCCTTAGGCACAACCCATGCAGATAATTTCTATGGTTCAATTCCTT
GTACAAATTCACTAACAGATCAACAAATTCAAGGTGAATATGAACATAATACCGGGCTTGTTATCCTCGATCACTTTCAA
AAAAACAAGCTTGATTATATCGGAACTCCAGCTGTTTTAGTCAAAGAACATGGTCCTTTTTGTTGGTCAAATAAATCTGC
TGAGGATGCAGTTAAACTTGCTATGACTTTCGAAGAAGTAGCCAAAATGGCTTTTTATACAAAAGTGATTAATCCTTACC
AAAACCAAGCAAATCCTATTCTCCAAAAAAAACATTATGAGCGCAAACACGGAAAAAACGCCTATTATGGACAAAAAAAG
TAG

Upstream 100 bases:

>100_bases
AATTTTGACCAAAATGCTGAATTTGAGCAAAATTTTCAAAAACAGGTTGAAATAATTCGTTCAGCCCGTGAATTTTTTCA
AGAAAAGTTGAGGTTAGCTA

Downstream 100 bases:

>100_bases
TCAAATTATCCCCAAAGATCTTCATAAACTAGCTCAGATTGATAATATCGTTTTTGATCTTGATGGGACCTTACTAACTT
CAGATCATGAAATCAGCCTT

Product: L-ribulose-5-phosphate 4-epimerase

Products: NA

Alternate protein names: L-ascorbate utilization protein F; Phosphoribulose isomerase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKIKDRNELEELQKAVLEANLLLYRSKLALHTWGNVSAISKDRSYYVIKPSGIPYEKMQFSDMVPVDLENNVLETNLNPS
SDTPTHSLIYKADSRIQAIVHTHSPFAVAWAQAGKDIPALGTTHADNFYGSIPCTNSLTDQQIQGEYEHNTGLVILDHFQ
KNKLDYIGTPAVLVKEHGPFCWSNKSAEDAVKLAMTFEEVAKMAFYTKVINPYQNQANPILQKKHYERKHGKNAYYGQKK

Sequences:

>Translated_240_residues
MKIKDRNELEELQKAVLEANLLLYRSKLALHTWGNVSAISKDRSYYVIKPSGIPYEKMQFSDMVPVDLENNVLETNLNPS
SDTPTHSLIYKADSRIQAIVHTHSPFAVA*AQAGKDIPALGTTHADNFYGSIPCTNSLTDQQIQGEYEHNTGLVILDHFQ
KNKLDYIGTPAVLVKEHGPFCWSNKSAEDAVKLAMTFEEVAKMAFYTKVINPYQNQANPILQKKHYERKHGKNAYYGQKK
>Mature_240_residues
MKIKDRNELEELQKAVLEANLLLYRSKLALHTWGNVSAISKDRSYYVIKPSGIPYEKMQFSDMVPVDLENNVLETNLNPS
SDTPTHSLIYKADSRIQAIVHTHSPFAVA*AQAGKDIPALGTTHADNFYGSIPCTNSLTDQQIQGEYEHNTGLVILDHFQ
KNKLDYIGTPAVLVKEHGPFCWSNKSAEDAVKLAMTFEEVAKMAFYTKVINPYQNQANPILQKKHYERKHGKNAYYGQKK

Specific function: Catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate. Is involved in the anaerobic L-ascorbate utilization [H]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. AraD/FucA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786247, Length=231, Percent_Identity=52.8138528138528, Blast_Score=235, Evalue=2e-63,
Organism=Escherichia coli, GI1790642, Length=231, Percent_Identity=51.5151515151515, Blast_Score=234, Evalue=5e-63,
Organism=Escherichia coli, GI1790008, Length=231, Percent_Identity=49.3506493506493, Blast_Score=216, Evalue=1e-57,

Paralogues:

None

Copy number: 136 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: =5.1.3.4 [H]

Molecular weight: Translated: 26981; Mature: 26981

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKDRNELEELQKAVLEANLLLYRSKLALHTWGNVSAISKDRSYYVIKPSGIPYEKMQF
CCCCCHHHHHHHHHHHHHHHHHHHEHHEEEEECCCEEEEECCCEEEEECCCCCCHHHHHH
SDMVPVDLENNVLETNLNPSSDTPTHSLIYKADSRIQAIVHTHSPFAVAAQAGKDIPALG
CCCCCCCCCCCEEECCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEHHCCCCCCCCC
TTHADNFYGSIPCTNSLTDQQIQGEYEHNTGLVILDHFQKNKLDYIGTPAVLVKEHGPFC
CCCCCCCEECCCCCCCCCCHHHCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCEE
WSNKSAEDAVKLAMTFEEVAKMAFYTKVINPYQNQANPILQKKHYERKHGKNAYYGQKK
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKIKDRNELEELQKAVLEANLLLYRSKLALHTWGNVSAISKDRSYYVIKPSGIPYEKMQF
CCCCCHHHHHHHHHHHHHHHHHHHEHHEEEEECCCEEEEECCCEEEEECCCCCCHHHHHH
SDMVPVDLENNVLETNLNPSSDTPTHSLIYKADSRIQAIVHTHSPFAVAAQAGKDIPALG
CCCCCCCCCCCEEECCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEHHCCCCCCCCC
TTHADNFYGSIPCTNSLTDQQIQGEYEHNTGLVILDHFQKNKLDYIGTPAVLVKEHGPFC
CCCCCCCEECCCCCCCCCCHHHCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCEE
WSNKSAEDAVKLAMTFEEVAKMAFYTKVINPYQNQANPILQKKHYERKHGKNAYYGQKK
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]