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The map label for this gene is pfkA [H]

Identifier: 71893464

GI number: 71893464

Start: 146026

End: 146994

Strand: Reverse

Name: pfkA [H]

Synonym: MHJ_0107

Alternate gene names: 71893464

Gene position: 146994-146026 (Counterclockwise)

Preceding gene: 71893472

Following gene: 71893463

Centisome position: 16.38

GC content: 34.26

Gene sequence:

>969_bases
ATGTCAAAAAAAATCGGTATATTAACTTCAGGCGGCGATGCGCCTGGGATGAATTCTGCAATAAGTTTTTTGGCAAAATC
AGCACTTAGTTTAGGTTTTGAGCCATATTTAATTTTTGATGGCTATTCTGGAATAATCGCAAGAAAAATATTGCCAGCAA
AAAATTTTCCTTATAATGGAATTTCTAGTTTTGGCGGAACTGCCATAGGATCAAGTCGCTTTCCTGAATTTAAAAAAGAG
GAAGTTCAAAATAAAGCCGTTGAAATTTTAAGTGAAATTGGGATTAGTTCGCTTGTGGTAGTTGGTGGCGATGGAACCTA
TAATGGCGGTTATAAATTACATTTAAAAGGAATAAAAGTAATCGCTTTACCAGGAACCATTGATAATGATATCCAGTTTA
CCGATTATACAATTGGATTTGATACTGCTTTGAATACGATAGTTGAAACTATTGATAAATTAAGGGATACGGCGAATTCT
CATCGTCGTTGTTTTGTAGTTGAAGTTATGGGGAGACATTGTCAAGATTTAGCTTTATATTCGGCAATGGCAACTGGAAG
CGAAATTTTAATTACTAATACAAATATTTTAACCCCTGAAGAGGTAAGTCAAAAAGTTTTAGAACAGTTTGCAAAAGGCA
AACCAAGCGTGATAGTAACAATTACAGAGAATATTTTACCAAACCTAAAAGAGTTTGCGGCTAAAATTGAGGAGTTAACA
AAAATTTCCACTCGCTCTTTAGAAGTTGGACATACCCAGCGCGGCGGCAGGCCTTCAGCTTTTGACCGAATTTTAGCAGC
CAAGATGGCAATGAAAGCTATGGAATTAATTAATCAAGATAAATCAGGTCTTGCAATTAGTTATTTAGATGGTAAAATCC
AAACTTTTGATATTGCGAAAGTAGTATCAAAACCTGTTAGAAAAACAAACGACTTAGTGCTTGAAATTAATAAAATCAAT
CAAAATTAA

Upstream 100 bases:

>100_bases
ATCATTGGGTCAGAGGTTCAAATCCTCTAGGGCGTACCATTTCAAATTTTTTGTCCTATTTTATTTATATATCTAAAAAA
GGAATTCTATTTAAGGTAAA

Downstream 100 bases:

>100_bases
AGGATTAAAATGCGAAAAATAGTAAAATTAAAAATGGCCAAAAGACGTGAACTAAGAAGACTTAAAACTTCAAAAGCGGC
AAAAAAAGCAAATGCGAAGT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSKKIGILTSGGDAPGMNSAISFLAKSALSLGFEPYLIFDGYSGIIARKILPAKNFPYNGISSFGGTAIGSSRFPEFKKE
EVQNKAVEILSEIGISSLVVVGGDGTYNGGYKLHLKGIKVIALPGTIDNDIQFTDYTIGFDTALNTIVETIDKLRDTANS
HRRCFVVEVMGRHCQDLALYSAMATGSEILITNTNILTPEEVSQKVLEQFAKGKPSVIVTITENILPNLKEFAAKIEELT
KISTRSLEVGHTQRGGRPSAFDRILAAKMAMKAMELINQDKSGLAISYLDGKIQTFDIAKVVSKPVRKTNDLVLEINKIN
QN

Sequences:

>Translated_322_residues
MSKKIGILTSGGDAPGMNSAISFLAKSALSLGFEPYLIFDGYSGIIARKILPAKNFPYNGISSFGGTAIGSSRFPEFKKE
EVQNKAVEILSEIGISSLVVVGGDGTYNGGYKLHLKGIKVIALPGTIDNDIQFTDYTIGFDTALNTIVETIDKLRDTANS
HRRCFVVEVMGRHCQDLALYSAMATGSEILITNTNILTPEEVSQKVLEQFAKGKPSVIVTITENILPNLKEFAAKIEELT
KISTRSLEVGHTQRGGRPSAFDRILAAKMAMKAMELINQDKSGLAISYLDGKIQTFDIAKVVSKPVRKTNDLVLEINKIN
QN
>Mature_321_residues
SKKIGILTSGGDAPGMNSAISFLAKSALSLGFEPYLIFDGYSGIIARKILPAKNFPYNGISSFGGTAIGSSRFPEFKKEE
VQNKAVEILSEIGISSLVVVGGDGTYNGGYKLHLKGIKVIALPGTIDNDIQFTDYTIGFDTALNTIVETIDKLRDTANSH
RRCFVVEVMGRHCQDLALYSAMATGSEILITNTNILTPEEVSQKVLEQFAKGKPSVIVTITENILPNLKEFAAKIEELTK
ISTRSLEVGHTQRGGRPSAFDRILAAKMAMKAMELINQDKSGLAISYLDGKIQTFDIAKVVSKPVRKTNDLVLEINKINQ
N

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=308, Percent_Identity=37.987012987013, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI48762920, Length=308, Percent_Identity=37.012987012987, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI266453768, Length=308, Percent_Identity=33.1168831168831, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI266453748, Length=308, Percent_Identity=33.1168831168831, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI4505749, Length=308, Percent_Identity=33.1168831168831, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI266453619, Length=308, Percent_Identity=33.1168831168831, Blast_Score=158, Evalue=6e-39,
Organism=Escherichia coli, GI1790350, Length=307, Percent_Identity=44.9511400651466, Blast_Score=261, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI25147584, Length=308, Percent_Identity=35.0649350649351, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17558788, Length=323, Percent_Identity=32.8173374613003, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI72003601, Length=291, Percent_Identity=27.8350515463918, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323861, Length=312, Percent_Identity=33.6538461538462, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6321679, Length=310, Percent_Identity=32.5806451612903, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI28573326, Length=305, Percent_Identity=35.7377049180328, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24652337, Length=305, Percent_Identity=35.0819672131148, Blast_Score=171, Evalue=8e-43,
Organism=Drosophila melanogaster, GI17647809, Length=305, Percent_Identity=35.0819672131148, Blast_Score=170, Evalue=1e-42,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 34952; Mature: 34821

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKIGILTSGGDAPGMNSAISFLAKSALSLGFEPYLIFDGYSGIIARKILPAKNFPYNG
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCCCCCC
ISSFGGTAIGSSRFPEFKKEEVQNKAVEILSEIGISSLVVVGGDGTYNGGYKLHLKGIKV
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECEEE
IALPGTIDNDIQFTDYTIGFDTALNTIVETIDKLRDTANSHRRCFVVEVMGRHCQDLALY
EEECCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHH
SAMATGSEILITNTNILTPEEVSQKVLEQFAKGKPSVIVTITENILPNLKEFAAKIEELT
HHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHCCCHHHHHHHHHHHH
KISTRSLEVGHTQRGGRPSAFDRILAAKMAMKAMELINQDKSGLAISYLDGKIQTFDIAK
HHHHCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEHHHHH
VVSKPVRKTNDLVLEINKINQN
HHHHHHCCCCCEEEEEEECCCC
>Mature Secondary Structure 
SKKIGILTSGGDAPGMNSAISFLAKSALSLGFEPYLIFDGYSGIIARKILPAKNFPYNG
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCCCCCC
ISSFGGTAIGSSRFPEFKKEEVQNKAVEILSEIGISSLVVVGGDGTYNGGYKLHLKGIKV
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECEEE
IALPGTIDNDIQFTDYTIGFDTALNTIVETIDKLRDTANSHRRCFVVEVMGRHCQDLALY
EEECCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHH
SAMATGSEILITNTNILTPEEVSQKVLEQFAKGKPSVIVTITENILPNLKEFAAKIEELT
HHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHCCCHHHHHHHHHHHH
KISTRSLEVGHTQRGGRPSAFDRILAAKMAMKAMELINQDKSGLAISYLDGKIQTFDIAK
HHHHCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEHHHHH
VVSKPVRKTNDLVLEINKINQN
HHHHHHCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA