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The map label for this gene is gyrB [H]

Identifier: 71893463

GI number: 71893463

Start: 143633

End: 145552

Strand: Reverse

Name: gyrB [H]

Synonym: MHJ_0106

Alternate gene names: 71893463

Gene position: 145552-143633 (Counterclockwise)

Preceding gene: 71893464

Following gene: 71893462

Centisome position: 16.22

GC content: 35.0

Gene sequence:

>1920_bases
ATGTCAAAAGATTATCAAGCCGAAAATATAGTTGTCTTAGAAGGTTTAGAAGCAGTTCGCAAACGCCCGGGAATGTATAT
CGGCGGGACCGGAATAAACGAGTTGCATCATTTGGTTTGAGAAATTGTTGATAATGCAGTTGATGAGGCTCTTGCAGGTT
TTGCATCCAAAATTAAAGTTACAATTTTGGATGATTTCAAAATCAGTATCGAGGATGACGGTCGCGGCATTCCGACTGGA
ATTCACCCAAAATATGAGATTTCAGCCGCTGAGGTTGTATTTACAGTTTTACATGCCGGTGGAAAATTCGATGGTAAATC
CTATAAGGTTTCAGGTGGTCTTCATGGAGTTGGGGCCTCGGTTGTTAATGCTTTATCCAAGGAATTAGAGGTTTTTATTA
AAAGGGATGGCAAACTTTGATACCAAAAATTCATTAACGGCGGCCAAAAAACAGCAGACCTAAGTGTAATTTCTAATCTT
GATAGTTCCCAAAGTGGGACTAAAATTATTTTTAGCCCCGATTTTGAGATTCTTGAAAAAAACAGTTTTCAATTACTTAC
AATTTTTAACCGTCTTAAAAATACAGCCTATTTAACACGTAGAATCCAATTTGAACTAATTGATAAACGGAGTGATTTTT
CCCAAACTTTCTGTTTTGAGGGCGGAATTTCTGATTATGTCGAAGAATTAAGTAAAAATTTTAAGAAATTAACTAATACA
ATCATTTATAGCGAGAATCAATATGAGCATAATCAGGATACAATTAAGGTTGAATTTGCCCTTAATTATGTCCAAGAAGA
TCATAGTCAAATTAAAAGTTATACAAACAATATTCATAACCCTGAAGGCGGAAGTCACGAAAATGGGCTTTGTGATGGCC
TTTTGCGTATTCTAAATAATTATGCACTTAGTAACAAATTTATCAAATCCGAAACTGATAAATTTGTCAAACAAGATATA
AAAGATGGCCTAATTGCAATCGTTTCAATTAAACATACTAACCCAATTTTTGAAGGTCAAACAAAGGCAAAATTAAACAA
CAAAGAGGTTCGTCAATTTGTAAATAAAGCTTTTAGCGAGGTTTTTCAAAGATATCTAGCCGAAAATCCTGAGGATGCCA
AGAAAATTATCGCCCGAAATCTGCTTGCACAAAAAGCAGCCAGAGCAGCAAAATCTGCCCGTGAGGCAATAAAACGTAAA
TCTGCTTTTGATATTGGGACTCTTCCAGGAAAACTTGCAGATTGTGGAACAAAAAATTCCGAGATCGCCGAACTTTATAT
TGTTGAGGGGAATTCAGCCGGGGGCAGTGCAAAAATGGGGCGTGACCGACATTTTCAGGCTATTTTACCTTTGCGGGGAA
AAGTTATTAATTCCCAACGGTTTCAGCTTGAAAAAGTACTTAAAAATGAAGAAATTCTATCGATGATCACCGCTTTTGGA
ACAGGAGTTGGTCCTGAATTTGATATTAGTAAAATCCGCTATCAAAAAATAATTATTATGACGGATGCTGACTCAGATGG
GTCGCATATTCGTTCACTTTTATTAACATTTTTGTTCCGTTATTTTAAAAAGCTAATTGAATTTGGCTTTGTTTATATTG
CGATCCCGCCTTTATATAAAATAAGTCAAGGAAAAAAAATTTTTTATGCCTATAATGAAAAAGAAAAAGAAAAATATTTA
ACAGAAATTTTAAAGGAAAATTCCAAATTTACTATCCAAAGATATAAAGGTTTAGGCGAAATGAATCCGGAACAACTTTG
AGAGACAACAATGGATCCCCAGACTAGAAGAATGTATCAAGTTCAAATTCATGATCTTGAAGTAGTCGCCCAGGTTTTTG
ATTCGTTAATGGGAACAGACGTAACAATGCGCAAAATTTTCATTGAAGAAAATGCGCAATATGCAAAAGTTGATATCTAG

Upstream 100 bases:

>100_bases
CTTTAAATTTAAAGCATTTTTATTCCTTTATATAAAAAAACCACCTGGCTTTTTGGAATTTTGTGGTATAATAATTAATT
TATAAATACTGGAGAAGCTA

Downstream 100 bases:

>100_bases
ATCTACTAGTTTAAATTTTTAAACTTTTTTGAAAAAAGTGCCTTAAAATGGGCATTTTTTTGTTTTTTTTGTTTTTTTTG
TTGCAAAAAAAAAAAAAAAA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 639; Mature: 638

Protein sequence:

>639_residues
MSKDYQAENIVVLEGLEAVRKRPGMYIGGTGINELHHLVWEIVDNAVDEALAGFASKIKVTILDDFKISIEDDGRGIPTG
IHPKYEISAAEVVFTVLHAGGKFDGKSYKVSGGLHGVGASVVNALSKELEVFIKRDGKLWYQKFINGGQKTADLSVISNL
DSSQSGTKIIFSPDFEILEKNSFQLLTIFNRLKNTAYLTRRIQFELIDKRSDFSQTFCFEGGISDYVEELSKNFKKLTNT
IIYSENQYEHNQDTIKVEFALNYVQEDHSQIKSYTNNIHNPEGGSHENGLCDGLLRILNNYALSNKFIKSETDKFVKQDI
KDGLIAIVSIKHTNPIFEGQTKAKLNNKEVRQFVNKAFSEVFQRYLAENPEDAKKIIARNLLAQKAARAAKSAREAIKRK
SAFDIGTLPGKLADCGTKNSEIAELYIVEGNSAGGSAKMGRDRHFQAILPLRGKVINSQRFQLEKVLKNEEILSMITAFG
TGVGPEFDISKIRYQKIIIMTDADSDGSHIRSLLLTFLFRYFKKLIEFGFVYIAIPPLYKISQGKKIFYAYNEKEKEKYL
TEILKENSKFTIQRYKGLGEMNPEQLWETTMDPQTRRMYQVQIHDLEVVAQVFDSLMGTDVTMRKIFIEENAQYAKVDI

Sequences:

>Translated_639_residues
MSKDYQAENIVVLEGLEAVRKRPGMYIGGTGINELHHLV*EIVDNAVDEALAGFASKIKVTILDDFKISIEDDGRGIPTG
IHPKYEISAAEVVFTVLHAGGKFDGKSYKVSGGLHGVGASVVNALSKELEVFIKRDGKL*YQKFINGGQKTADLSVISNL
DSSQSGTKIIFSPDFEILEKNSFQLLTIFNRLKNTAYLTRRIQFELIDKRSDFSQTFCFEGGISDYVEELSKNFKKLTNT
IIYSENQYEHNQDTIKVEFALNYVQEDHSQIKSYTNNIHNPEGGSHENGLCDGLLRILNNYALSNKFIKSETDKFVKQDI
KDGLIAIVSIKHTNPIFEGQTKAKLNNKEVRQFVNKAFSEVFQRYLAENPEDAKKIIARNLLAQKAARAAKSAREAIKRK
SAFDIGTLPGKLADCGTKNSEIAELYIVEGNSAGGSAKMGRDRHFQAILPLRGKVINSQRFQLEKVLKNEEILSMITAFG
TGVGPEFDISKIRYQKIIIMTDADSDGSHIRSLLLTFLFRYFKKLIEFGFVYIAIPPLYKISQGKKIFYAYNEKEKEKYL
TEILKENSKFTIQRYKGLGEMNPEQL*ETTMDPQTRRMYQVQIHDLEVVAQVFDSLMGTDVTMRKIFIEENAQYAKVDI
>Mature_638_residues
SKDYQAENIVVLEGLEAVRKRPGMYIGGTGINELHHLV*EIVDNAVDEALAGFASKIKVTILDDFKISIEDDGRGIPTGI
HPKYEISAAEVVFTVLHAGGKFDGKSYKVSGGLHGVGASVVNALSKELEVFIKRDGKL*YQKFINGGQKTADLSVISNLD
SSQSGTKIIFSPDFEILEKNSFQLLTIFNRLKNTAYLTRRIQFELIDKRSDFSQTFCFEGGISDYVEELSKNFKKLTNTI
IYSENQYEHNQDTIKVEFALNYVQEDHSQIKSYTNNIHNPEGGSHENGLCDGLLRILNNYALSNKFIKSETDKFVKQDIK
DGLIAIVSIKHTNPIFEGQTKAKLNNKEVRQFVNKAFSEVFQRYLAENPEDAKKIIARNLLAQKAARAAKSAREAIKRKS
AFDIGTLPGKLADCGTKNSEIAELYIVEGNSAGGSAKMGRDRHFQAILPLRGKVINSQRFQLEKVLKNEEILSMITAFGT
GVGPEFDISKIRYQKIIIMTDADSDGSHIRSLLLTFLFRYFKKLIEFGFVYIAIPPLYKISQGKKIFYAYNEKEKEKYLT
EILKENSKFTIQRYKGLGEMNPEQL*ETTMDPQTRRMYQVQIHDLEVVAQVFDSLMGTDVTMRKIFIEENAQYAKVDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=612, Percent_Identity=27.4509803921569, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI19913406, Length=564, Percent_Identity=26.063829787234, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI48994957, Length=568, Percent_Identity=46.4788732394366, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1789408, Length=650, Percent_Identity=38.7692307692308, Blast_Score=450, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI212645845, Length=544, Percent_Identity=27.5735294117647, Blast_Score=153, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17535065, Length=550, Percent_Identity=26.7272727272727, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI212645657, Length=193, Percent_Identity=30.0518134715026, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6324241, Length=667, Percent_Identity=27.8860569715142, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI17136538, Length=537, Percent_Identity=26.2569832402235, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71744; Mature: 71613

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDYQAENIVVLEGLEAVRKRPGMYIGGTGINELHHLVEIVDNAVDEALAGFASKIKVT
CCCCCCCCCEEEECCHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
ILDDFKISIEDDGRGIPTGIHPKYEISAAEVVFTVLHAGGKFDGKSYKVSGGLHGVGASV
EEECEEEEECCCCCCCCCCCCCCEECHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHH
VNALSKELEVFIKRDGKLYQKFINGGQKTADLSVISNLDSSQSGTKIIFSPDFEILEKNS
HHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEECCCHHHHCCCC
FQLLTIFNRLKNTAYLTRRIQFELIDKRSDFSQTFCFEGGISDYVEELSKNFKKLTNTII
CHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHEE
YSENQYEHNQDTIKVEFALNYVQEDHSQIKSYTNNIHNPEGGSHENGLCDGLLRILNNYA
ECCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
LSNKFIKSETDKFVKQDIKDGLIAIVSIKHTNPIFEGQTKAKLNNKEVRQFVNKAFSEVF
HHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHHHHHH
QRYLAENPEDAKKIIARNLLAQKAARAAKSAREAIKRKSAFDIGTLPGKLADCGTKNSEI
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCE
AELYIVEGNSAGGSAKMGRDRHFQAILPLRGKVINSQRFQLEKVLKNEEILSMITAFGTG
EEEEEEECCCCCCCCCCCCCCCCEEECCCCCEECCCHHHHHHHHHCCHHHHHHHHHHCCC
VGPEFDISKIRYQKIIIMTDADSDGSHIRSLLLTFLFRYFKKLIEFGFVYIAIPPLYKIS
CCCCCCHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEECC
QGKKIFYAYNEKEKEKYLTEILKENSKFTIQRYKGLGEMNPEQLETTMDPQTRRMYQVQI
CCCEEEEEECCHHHHHHHHHHHHCCCCEEEHHHCCCCCCCHHHHHHCCCHHHHEEEEEEH
HDLEVVAQVFDSLMGTDVTMRKIFIEENAQYAKVDI
HHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEECC
>Mature Secondary Structure 
SKDYQAENIVVLEGLEAVRKRPGMYIGGTGINELHHLVEIVDNAVDEALAGFASKIKVT
CCCCCCCCEEEECCHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
ILDDFKISIEDDGRGIPTGIHPKYEISAAEVVFTVLHAGGKFDGKSYKVSGGLHGVGASV
EEECEEEEECCCCCCCCCCCCCCEECHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHH
VNALSKELEVFIKRDGKLYQKFINGGQKTADLSVISNLDSSQSGTKIIFSPDFEILEKNS
HHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEECCCHHHHCCCC
FQLLTIFNRLKNTAYLTRRIQFELIDKRSDFSQTFCFEGGISDYVEELSKNFKKLTNTII
CHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHEE
YSENQYEHNQDTIKVEFALNYVQEDHSQIKSYTNNIHNPEGGSHENGLCDGLLRILNNYA
ECCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
LSNKFIKSETDKFVKQDIKDGLIAIVSIKHTNPIFEGQTKAKLNNKEVRQFVNKAFSEVF
HHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHHHHHH
QRYLAENPEDAKKIIARNLLAQKAARAAKSAREAIKRKSAFDIGTLPGKLADCGTKNSEI
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCE
AELYIVEGNSAGGSAKMGRDRHFQAILPLRGKVINSQRFQLEKVLKNEEILSMITAFGTG
EEEEEEECCCCCCCCCCCCCCCCEEECCCCCEECCCHHHHHHHHHCCHHHHHHHHHHCCC
VGPEFDISKIRYQKIIIMTDADSDGSHIRSLLLTFLFRYFKKLIEFGFVYIAIPPLYKIS
CCCCCCHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEECC
QGKKIFYAYNEKEKEKYLTEILKENSKFTIQRYKGLGEMNPEQLETTMDPQTRRMYQVQI
CCCEEEEEECCHHHHHHHHHHHHCCCCEEEHHHCCCCCCCHHHHHHCCCHHHHEEEEEEH
HDLEVVAQVFDSLMGTDVTMRKIFIEENAQYAKVDI
HHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7521872 [H]