Click here to switch to the map view.

The map label for this gene is dsbA [H]

Identifier: 71892387

GI number: 71892387

Start: 772648

End: 773277

Strand: Reverse

Name: dsbA [H]

Synonym: BPEN_646

Alternate gene names: 71892387

Gene position: 773277-772648 (Counterclockwise)

Preceding gene: 71892388

Following gene: 71892386

Centisome position: 97.68

GC content: 27.14

Gene sequence:

>630_bases
ATGAAAAAATTGCATCTTATTTGGATAGTAATTCATTTGATATTTTCATGTAAAAGCATTGCATCTGCAATTATAGAAGG
CAGGCAATACATTCGATTAAATAAACCTATTCACAATGCACCTCAATTATTGGAATTTTTTTCTTTTTACTGTCCACATT
GCTATCAATTCGAAGAAATTTATCATATTTCATATAATATAGAACAAGCATTGCCTAAAAATATAAATTTTTATAAATAT
CATGTGAATTTTCTAGGTAATTTAGGAAAACAATTGACACATGCTTGGGCTGTAGCAATAGCATTAAGAATAGAAGATAG
AATCAGCCCTATATTATTTACAGCGATACAAAAACAACAATCTATTCATACTGTAGAAGATATTCGTGAAATATTTATTA
AATCTGGTGTTAATGCAGAAAAATTTGATGTTGCTTGGGATAGCGTATTAGTGAAATCTTTAATTTTAGATCAGGAACAA
GCAGCAATAAATTTTCGTTTGAGAGGAGTACCATCAATATTTATCAATGGGAAATATATGATAAAAAACGAGAAGTTAGA
TATATCATCAGTTAATGCTTATATAAAACAATTTTGTGAGTTGTTAAATTTACTTATAGATAAAACGTAA

Upstream 100 bases:

>100_bases
ATTAATTTTAATATTAACTAATTCAGAACAGTATTCTATCATAATATTACAATATTTATATTTTTATATGAAATATTTAT
GTATATATTAGGAGGGGGGT

Downstream 100 bases:

>100_bases
TTTTTGTATATTCATAATATCATCATACTGGTATATGCAAGTATTCTATTTTTTATTGAAATTATTTTACGCAATGACTG
CATTGATTTTATTATTTTGT

Product: periplasmic protein disulfide isomerase I

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MKKLHLIWIVIHLIFSCKSIASAIIEGRQYIRLNKPIHNAPQLLEFFSFYCPHCYQFEEIYHISYNIEQALPKNINFYKY
HVNFLGNLGKQLTHAWAVAIALRIEDRISPILFTAIQKQQSIHTVEDIREIFIKSGVNAEKFDVAWDSVLVKSLILDQEQ
AAINFRLRGVPSIFINGKYMIKNEKLDISSVNAYIKQFCELLNLLIDKT

Sequences:

>Translated_209_residues
MKKLHLIWIVIHLIFSCKSIASAIIEGRQYIRLNKPIHNAPQLLEFFSFYCPHCYQFEEIYHISYNIEQALPKNINFYKY
HVNFLGNLGKQLTHAWAVAIALRIEDRISPILFTAIQKQQSIHTVEDIREIFIKSGVNAEKFDVAWDSVLVKSLILDQEQ
AAINFRLRGVPSIFINGKYMIKNEKLDISSVNAYIKQFCELLNLLIDKT
>Mature_209_residues
MKKLHLIWIVIHLIFSCKSIASAIIEGRQYIRLNKPIHNAPQLLEFFSFYCPHCYQFEEIYHISYNIEQALPKNINFYKY
HVNFLGNLGKQLTHAWAVAIALRIEDRISPILFTAIQKQQSIHTVEDIREIFIKSGVNAEKFDVAWDSVLVKSLILDQEQ
AAINFRLRGVPSIFINGKYMIKNEKLDISSVNAYIKQFCELLNLLIDKT

Specific function: Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. It is required for pilus biogenesis [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=210, Percent_Identity=46.6666666666667, Blast_Score=229, Evalue=1e-61,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 24342; Mature: 24342

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLHLIWIVIHLIFSCKSIASAIIEGRQYIRLNKPIHNAPQLLEFFSFYCPHCYQFEEI
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHHHHHCCCCCCHHHH
YHISYNIEQALPKNINFYKYHVNFLGNLGKQLTHAWAVAIALRIEDRISPILFTAIQKQQ
EEEEECHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIHTVEDIREIFIKSGVNAEKFDVAWDSVLVKSLILDQEQAAINFRLRGVPSIFINGKYM
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHEEEEECCCCEEEECCEEE
IKNEKLDISSVNAYIKQFCELLNLLIDKT
EECCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKLHLIWIVIHLIFSCKSIASAIIEGRQYIRLNKPIHNAPQLLEFFSFYCPHCYQFEEI
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHHHHHCCCCCCHHHH
YHISYNIEQALPKNINFYKYHVNFLGNLGKQLTHAWAVAIALRIEDRISPILFTAIQKQQ
EEEEECHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIHTVEDIREIFIKSGVNAEKFDVAWDSVLVKSLILDQEQAAINFRLRGVPSIFINGKYM
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHEEEEECCCCEEEECCEEE
IKNEKLDISSVNAYIKQFCELLNLLIDKT
EECCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10463161 [H]