| Definition | Candidatus Blochmannia pennsylvanicus str. BPEN, complete genome. |
|---|---|
| Accession | NC_007292 |
| Length | 791,654 |
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The map label for this gene is glnA [H]
Identifier: 71892384
GI number: 71892384
Start: 767280
End: 768704
Strand: Direct
Name: glnA [H]
Synonym: BPEN_643
Alternate gene names: 71892384
Gene position: 767280-768704 (Clockwise)
Preceding gene: 71892383
Following gene: 71892385
Centisome position: 96.92
GC content: 32.98
Gene sequence:
>1425_bases ATGACAAATATTGAAAAAATTTTTTCTATTATCAACGAAAATCAAATAAAATTTGTTGATTTGCGTTTTACTGATACAAA AGGAAAAGAACAACACATTACTATCCCTAAGACACAGGTTAATGATCATTTATTTAAACATGGAAAAATTTTTGATGGGT CCTCCATTAAGGGATGGAAAGATGTTAGCAAATCAGACATGATATTAATGCCTGATCTTACTAGTTTTGTGATAGATCCA TTTTATGAAGATGCTACAATAATTATTCGTTGCGATATCATTGATCCTAGTACTATGAAAAATTATGATAGAGATCCACG GTCTATAGCTAAACGAGCAGAAATGTTTTTGTATAATTCTGGTATTGCCGATATTGTTATGTTTGGTCCTGAACCAGAAT TTTTTTTATTTGACAGTATTCATTTTGCAACAACAATATCAGGATCGTATGTAATCGTCGACGATCGGGAATCATCATGG AACAGTGGTAAATTTTATAAAGATGGGAATAAAGGTCATCGTCCTAGAATAAAGAGTGGATATGCCCCGGTGCCCCCAAT AGATTCTTCTCAAAACTTAAGATCTACCATGTCTTTAACAATGGAAAAAATGGGATTGATTGTAGAGGCTCATCATCATG AAGTTGCAACATCGGGTCAAAATGAAATAGCAACTCGATTTAATACTCTTACTAAAAAAGCTGATGAAATACAAATATAT AAATATGTAGTACACAATGTCGCTCATAATTCTGGGAAAACTGCAACTTTTATGCCTAAACCCATTATAAATGATAATGG GTCAGGCATGCATTGCCATATATCCTTACATAAAAAAGATACTAATTTATTTTCAGGTCTTCAATATGGCGACCTTTCTG ATATTGCTTTATTTTATATTGGTGGTATTTTGCGTCACGCTAAAGCATTAAATGCTATAACCAATCCAACTACTAATTCG TATAAACGTTTAGTTCCAAATTATGAAGCTCCAGTAATGTTGACTTATTCTTCTTGTAATCGTTCTAGCGCGATTCGCAT TCCATCATCTCTAGAAAAATCAAAAAACGACGCAGCATGTCGCGTTGAAGTTCGTTTTCCTGATCCTTCTGCTAATCCTT ATCTAGCGTTTTCTGCATTGCTTATGGCCGGATTAGATGGCATTATCAATAAAATTCATCCTGGAGATCCTATTGATAAA AACTTATATACTATTTCTGAAAAAGAAGCATTAGCCATACCGCACATGGCTAAATCTCTTGACGAAGCATTAAAATCTTT ATCTGAAGATCATAAATTTTTAACTCGAGGCAACGTCTTCACCAGAGAGTATATTAATACATATATTTTATTGTGTCAAA AAGACAGTAGATTAGTATCTACTACGCCACATCCTGTAGAATTTGATCTATATTATAGTGTTTAA
Upstream 100 bases:
>100_bases TTTTGAAAAATGTTGATCATAAGATCCTTATAGATAGTATTTTATAGCGCATGATTTAGCAATATTATCAGCATATGATA TAATAACTGAGAGTATAAAT
Downstream 100 bases:
>100_bases AAACAATTTTTTGTAAAATATGTAAAAATACGTATATAACAATATTATTGTTGGTTTTTGATAATTCAAAAAATTTTATT AAAAATAAAAACAATTTGTT
Product: glutamine synthetase
Products: NA
Alternate protein names: Glutamate--ammonia ligase [H]
Number of amino acids: Translated: 474; Mature: 473
Protein sequence:
>474_residues MTNIEKIFSIINENQIKFVDLRFTDTKGKEQHITIPKTQVNDHLFKHGKIFDGSSIKGWKDVSKSDMILMPDLTSFVIDP FYEDATIIIRCDIIDPSTMKNYDRDPRSIAKRAEMFLYNSGIADIVMFGPEPEFFLFDSIHFATTISGSYVIVDDRESSW NSGKFYKDGNKGHRPRIKSGYAPVPPIDSSQNLRSTMSLTMEKMGLIVEAHHHEVATSGQNEIATRFNTLTKKADEIQIY KYVVHNVAHNSGKTATFMPKPIINDNGSGMHCHISLHKKDTNLFSGLQYGDLSDIALFYIGGILRHAKALNAITNPTTNS YKRLVPNYEAPVMLTYSSCNRSSAIRIPSSLEKSKNDAACRVEVRFPDPSANPYLAFSALLMAGLDGIINKIHPGDPIDK NLYTISEKEALAIPHMAKSLDEALKSLSEDHKFLTRGNVFTREYINTYILLCQKDSRLVSTTPHPVEFDLYYSV
Sequences:
>Translated_474_residues MTNIEKIFSIINENQIKFVDLRFTDTKGKEQHITIPKTQVNDHLFKHGKIFDGSSIKGWKDVSKSDMILMPDLTSFVIDP FYEDATIIIRCDIIDPSTMKNYDRDPRSIAKRAEMFLYNSGIADIVMFGPEPEFFLFDSIHFATTISGSYVIVDDRESSW NSGKFYKDGNKGHRPRIKSGYAPVPPIDSSQNLRSTMSLTMEKMGLIVEAHHHEVATSGQNEIATRFNTLTKKADEIQIY KYVVHNVAHNSGKTATFMPKPIINDNGSGMHCHISLHKKDTNLFSGLQYGDLSDIALFYIGGILRHAKALNAITNPTTNS YKRLVPNYEAPVMLTYSSCNRSSAIRIPSSLEKSKNDAACRVEVRFPDPSANPYLAFSALLMAGLDGIINKIHPGDPIDK NLYTISEKEALAIPHMAKSLDEALKSLSEDHKFLTRGNVFTREYINTYILLCQKDSRLVSTTPHPVEFDLYYSV >Mature_473_residues TNIEKIFSIINENQIKFVDLRFTDTKGKEQHITIPKTQVNDHLFKHGKIFDGSSIKGWKDVSKSDMILMPDLTSFVIDPF YEDATIIIRCDIIDPSTMKNYDRDPRSIAKRAEMFLYNSGIADIVMFGPEPEFFLFDSIHFATTISGSYVIVDDRESSWN SGKFYKDGNKGHRPRIKSGYAPVPPIDSSQNLRSTMSLTMEKMGLIVEAHHHEVATSGQNEIATRFNTLTKKADEIQIYK YVVHNVAHNSGKTATFMPKPIINDNGSGMHCHISLHKKDTNLFSGLQYGDLSDIALFYIGGILRHAKALNAITNPTTNSY KRLVPNYEAPVMLTYSSCNRSSAIRIPSSLEKSKNDAACRVEVRFPDPSANPYLAFSALLMAGLDGIINKIHPGDPIDKN LYTISEKEALAIPHMAKSLDEALKSLSEDHKFLTRGNVFTREYINTYILLCQKDSRLVSTTPHPVEFDLYYSV
Specific function: Unknown
COG id: COG0174
COG function: function code E; Glutamine synthetase
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glutamine synthetase family [H]
Homologues:
Organism=Escherichia coli, GI1790301, Length=470, Percent_Identity=65.7446808510638, Blast_Score=673, Evalue=0.0, Organism=Escherichia coli, GI87081870, Length=201, Percent_Identity=29.8507462686567, Blast_Score=81, Evalue=2e-16,
Paralogues:
None
Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR008147 - InterPro: IPR014746 - InterPro: IPR008146 - InterPro: IPR004809 - InterPro: IPR001637 [H]
Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]
EC number: =6.3.1.2 [H]
Molecular weight: Translated: 53380; Mature: 53249
Theoretical pI: Translated: 7.51; Mature: 7.51
Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 3.0 %Met (Translated Protein) 4.0 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 3.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTNIEKIFSIINENQIKFVDLRFTDTKGKEQHITIPKTQVNDHLFKHGKIFDGSSIKGWK CCCHHHHHHHHCCCCEEEEEEEEECCCCCCCEEECCCCHHHHHHHHCCCEECCCCCCCHH DVSKSDMILMPDLTSFVIDPFYEDATIIIRCDIIDPSTMKNYDRDPRSIAKRAEMFLYNS CCCCCCEEECCCHHHHHHCCCCCCCEEEEEEEECCCHHHHCCCCCHHHHHHHHHHEEEEC GIADIVMFGPEPEFFLFDSIHFATTISGSYVIVDDRESSWNSGKFYKDGNKGHRPRIKSG CCEEEEEECCCCCEEEEECEEEEEEECCCEEEEECCCCCCCCCCEEECCCCCCCCCCCCC YAPVPPIDSSQNLRSTMSLTMEKMGLIVEAHHHEVATSGQNEIATRFNTLTKKADEIQIY CCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCHHHCCCCHHHHHHHHHHHCCCHHHHHH KYVVHNVAHNSGKTATFMPKPIINDNGSGMHCHISLHKKDTNLFSGLQYGDLSDIALFYI HHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCHHHCCCCCCCHHHHHHHHH GGILRHAKALNAITNPTTNSYKRLVPNYEAPVMLTYSSCNRSSAIRIPSSLEKSKNDAAC HHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEECCCCCCCEEECCHHHHCCCCCCEE RVEVRFPDPSANPYLAFSALLMAGLDGIINKIHPGDPIDKNLYTISEKEALAIPHMAKSL EEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEECHHHHHHH DEALKSLSEDHKFLTRGNVFTREYINTYILLCQKDSRLVSTTPHPVEFDLYYSV HHHHHHHHHHHHEECCCCCHHHHHCCEEEEEECCCCCEEECCCCCEEEEEEEEC >Mature Secondary Structure TNIEKIFSIINENQIKFVDLRFTDTKGKEQHITIPKTQVNDHLFKHGKIFDGSSIKGWK CCHHHHHHHHCCCCEEEEEEEEECCCCCCCEEECCCCHHHHHHHHCCCEECCCCCCCHH DVSKSDMILMPDLTSFVIDPFYEDATIIIRCDIIDPSTMKNYDRDPRSIAKRAEMFLYNS CCCCCCEEECCCHHHHHHCCCCCCCEEEEEEEECCCHHHHCCCCCHHHHHHHHHHEEEEC GIADIVMFGPEPEFFLFDSIHFATTISGSYVIVDDRESSWNSGKFYKDGNKGHRPRIKSG CCEEEEEECCCCCEEEEECEEEEEEECCCEEEEECCCCCCCCCCEEECCCCCCCCCCCCC YAPVPPIDSSQNLRSTMSLTMEKMGLIVEAHHHEVATSGQNEIATRFNTLTKKADEIQIY CCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCHHHCCCCHHHHHHHHHHHCCCHHHHHH KYVVHNVAHNSGKTATFMPKPIINDNGSGMHCHISLHKKDTNLFSGLQYGDLSDIALFYI HHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCHHHCCCCCCCHHHHHHHHH GGILRHAKALNAITNPTTNSYKRLVPNYEAPVMLTYSSCNRSSAIRIPSSLEKSKNDAAC HHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEECCCCCCCEEECCHHHHCCCCCCEE RVEVRFPDPSANPYLAFSALLMAGLDGIINKIHPGDPIDKNLYTISEKEALAIPHMAKSL EEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEECHHHHHHH DEALKSLSEDHKFLTRGNVFTREYINTYILLCQKDSRLVSTTPHPVEFDLYYSV HHHHHHHHHHHHEECCCCCHHHHHCCEEEEEECCCCCEEECCCCCEEEEEEEEC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796 [H]