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The map label for this gene is tpiA

Identifier: 71892364

GI number: 71892364

Start: 745741

End: 746517

Strand: Direct

Name: tpiA

Synonym: BPEN_623

Alternate gene names: 71892364

Gene position: 745741-746517 (Clockwise)

Preceding gene: 71892363

Following gene: 71892371

Centisome position: 94.2

GC content: 31.27

Gene sequence:

>777_bases
GTGAGACGTCTATTGATAATAGGAAACTGGAAATTAAATGGCAATAAAAATACCATAACTAATTTAATTATTACATTAGT
TAATACATTTTACAATATTTCTAAATGTAATGTAGCTATAGCTCCGCCCGTAATGTATTTAGATATAACTAAGCGTTATT
TATTGAACAGTCGTATTCAGTTATGTGCTCAAAATGTTGATATTCATTTATCTGGCTCGTTTACAGGCGATATTTCAGCA
GAAATGTTACAAGATCTTAATGTACGATATGCTCTGATAGGTCATTCTGAACGGAGAATACATCATAAAGAAAATGATGC
ATATATTGCTAAAAAATTTTTTATATTAAAAAAAGTTGGATTAATTCCTATTTTATGTGTAGGGGAAAATAAAAGAGAAT
ACGATTCTGGATACACACAGTCAGTATGCATTAATCAAATTAACACAATCATTACGTTACTTGGCATAGAAGCATTTAAG
AATTCAGTTATTGCTTATGAGCCTATATGGGCTATAGGAAGTGGTGCTAGCGCATCTCCAGAAAATGCGCAATTAGTTCA
TAAATCAATCCGGGATTATATTGCAAGTTATGATACATCTATAGCCGATAAAATAACGATTCAGTATGGAGGTTCTGTCA
CACCAGAAAATGTTACTAAATTTTTTGATCAAAAAGATATTGATGGTGTATTAGTAGGAGCCGCTTCTTTAAACGCGAAT
AGTTTTTCTATGATCGTACAAACTGCTGAAAACCATAAGAAATCATATCCTGCTTAA

Upstream 100 bases:

>100_bases
TAATCGTGTAATACATAGCATTATTTTGTTTAATGTTAATTAATGTTGATTTATGTATCACAAATGGTTGTTATAAAGTA
ATTTCTGTGGGAGAGATGAT

Downstream 100 bases:

>100_bases
TAATATGCAGACATATCCTATATATAGCATCATATATTATTGATGTAACGATATTAATAGTTCAAAACACTAAATAAGTG
ATATTTATATAAGCAAAGAT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MRRLLIIGNWKLNGNKNTITNLIITLVNTFYNISKCNVAIAPPVMYLDITKRYLLNSRIQLCAQNVDIHLSGSFTGDISA
EMLQDLNVRYALIGHSERRIHHKENDAYIAKKFFILKKVGLIPILCVGENKREYDSGYTQSVCINQINTIITLLGIEAFK
NSVIAYEPIWAIGSGASASPENAQLVHKSIRDYIASYDTSIADKITIQYGGSVTPENVTKFFDQKDIDGVLVGAASLNAN
SFSMIVQTAENHKKSYPA

Sequences:

>Translated_258_residues
MRRLLIIGNWKLNGNKNTITNLIITLVNTFYNISKCNVAIAPPVMYLDITKRYLLNSRIQLCAQNVDIHLSGSFTGDISA
EMLQDLNVRYALIGHSERRIHHKENDAYIAKKFFILKKVGLIPILCVGENKREYDSGYTQSVCINQINTIITLLGIEAFK
NSVIAYEPIWAIGSGASASPENAQLVHKSIRDYIASYDTSIADKITIQYGGSVTPENVTKFFDQKDIDGVLVGAASLNAN
SFSMIVQTAENHKKSYPA
>Mature_258_residues
MRRLLIIGNWKLNGNKNTITNLIITLVNTFYNISKCNVAIAPPVMYLDITKRYLLNSRIQLCAQNVDIHLSGSFTGDISA
EMLQDLNVRYALIGHSERRIHHKENDAYIAKKFFILKKVGLIPILCVGENKREYDSGYTQSVCINQINTIITLLGIEAFK
NSVIAYEPIWAIGSGASASPENAQLVHKSIRDYIASYDTSIADKITIQYGGSVTPENVTKFFDQKDIDGVLVGAASLNAN
SFSMIVQTAENHKKSYPA

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=245, Percent_Identity=37.5510204081633, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI4507645, Length=245, Percent_Identity=37.5510204081633, Blast_Score=166, Evalue=2e-41,
Organism=Escherichia coli, GI1790353, Length=255, Percent_Identity=52.156862745098, Blast_Score=297, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI17536593, Length=245, Percent_Identity=39.1836734693878, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6320255, Length=247, Percent_Identity=38.8663967611336, Blast_Score=174, Evalue=9e-45,
Organism=Drosophila melanogaster, GI28572008, Length=245, Percent_Identity=37.9591836734694, Blast_Score=165, Evalue=3e-41,
Organism=Drosophila melanogaster, GI28572006, Length=245, Percent_Identity=37.9591836734694, Blast_Score=165, Evalue=3e-41,
Organism=Drosophila melanogaster, GI28572004, Length=245, Percent_Identity=37.9591836734694, Blast_Score=164, Evalue=6e-41,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_BLOPB (Q491Y3)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_278098.1
- HSSP:   P36204
- ProteinModelPortal:   Q491Y3
- SMR:   Q491Y3
- STRING:   Q491Y3
- GeneID:   3563231
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_623
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   CBLO291272:BPEN_623-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 28691; Mature: 28691

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 167-167 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLLIIGNWKLNGNKNTITNLIITLVNTFYNISKCNVAIAPPVMYLDITKRYLLNSRIQ
CCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEHHHHHHHHHHHHH
LCAQNVDIHLSGSFTGDISAEMLQDLNVRYALIGHSERRIHHKENDAYIAKKFFILKKVG
HHHCCCCEEEECCCCCCCHHHHHHCCCCEEEEECCCHHHCCCCCCCHHHHHHHHHHHHCC
LIPILCVGENKREYDSGYTQSVCINQINTIITLLGIEAFKNSVIAYEPIWAIGSGASASP
CEEEEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEEECCCCCCCC
ENAQLVHKSIRDYIASYDTSIADKITIQYGGSVTPENVTKFFDQKDIDGVLVGAASLNAN
CHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCC
SFSMIVQTAENHKKSYPA
CHHHEEEEHHHCCCCCCC
>Mature Secondary Structure
MRRLLIIGNWKLNGNKNTITNLIITLVNTFYNISKCNVAIAPPVMYLDITKRYLLNSRIQ
CCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEHHHHHHHHHHHHH
LCAQNVDIHLSGSFTGDISAEMLQDLNVRYALIGHSERRIHHKENDAYIAKKFFILKKVG
HHHCCCCEEEECCCCCCCHHHHHHCCCCEEEEECCCHHHCCCCCCCHHHHHHHHHHHHCC
LIPILCVGENKREYDSGYTQSVCINQINTIITLLGIEAFKNSVIAYEPIWAIGSGASASP
CEEEEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEEECCCCCCCC
ENAQLVHKSIRDYIASYDTSIADKITIQYGGSVTPENVTKFFDQKDIDGVLVGAASLNAN
CHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCC
SFSMIVQTAENHKKSYPA
CHHHEEEEHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA