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The map label for this gene is dapE

Identifier: 71892287

GI number: 71892287

Start: 639918

End: 641072

Strand: Direct

Name: dapE

Synonym: BPEN_534

Alternate gene names: 71892287

Gene position: 639918-641072 (Clockwise)

Preceding gene: 71892286

Following gene: 71892290

Centisome position: 80.83

GC content: 32.73

Gene sequence:

>1155_bases
ATGAATTATTCAGCGCTAATAACATTAGCTCAAAAATTAATTCAGCAACCTTCGGTTAGCCCAAACCATCATAGTTGTCA
TGAAATAATTGCAAATTATTTAGAAAAACTAAATTTTAATGTAGAGTTAATGCGCTTTGATAACACCCTTAATCTTTGGG
CATTTCATAGTTGTAAAAAACAACAAAAACACACCACATTATTGTTTATTGGGCATACTGATGTGGTCGATCCTGGAGAT
CCTCAATTCTGGGATTACCCTCCGTTCTCAGGATTAGTACATAATAATATGTTACATGGGCGAGGCGCAATTGATATGAA
GGGGGCATTAGCAGCTATGTTAGTAGCCACTGCCAATTTTATCGACCAATATCCTAATTATCAAGGACGGATTGCATTTC
TTATCACTTCTGACGAAGAAGGCAGTGGTATCAACGGAACCACAAAAGTTGTAGAATCTCTAATTGCACGTAATGAACAT
ATAAACTATTGCATAGTCGGAGAACCATCCAGTCAAGATCAACTAGGTGATGTTATAAAAAATGGTAGACGTGGATCACT
TACTGGACAACTGACAATACACGGATCTCAAGGACATGTAGCCTATCCTCAATTCTCTAAAAATCCAATACATTTAATCA
TTCCAGCACTGTCAGATTTGCTAAACATTACATGGGATCGAGAAAAAAGTATATTATTTCCTCCTACTGCTATACAAATT
ACTAATATTTATTCTAATCATAAGAACAACAATGTTACACCCCATACAGTTATATTAAATTTTAATGTACGCTTTAATGA
TAAATGTTCTATTGACAATATTAAAAGATACATAAATGACATATTCACACGTCATACTTTACCTTATAATATCAATTGGA
AACTTTCTGCAGAGCCTTATTTTAGTAAACCTGGACAATTAACTAACGTTGCAATCAATGCAATTAAATATTACCAAAAA
TTTGAACCTCGATTAGAAACTACAGGAGGTACTTCTGATGGACGTTTTATAGCTAAAATGGGAACAGAAGTCATAGAATT
AGGAGCGCGTAACCATATGATTCATAAAGTTAACGAATACATTGATCTAATTGATTTAAAATTACTTAGCTCTATATATA
AAAAAATTATAGAAGATTTAATACTAATACAATAA

Upstream 100 bases:

>100_bases
GTATATCTGAATTTTACATTGATTATATATATGATTTATTCGTGTGAATTATTAAATTCAAAATAATTTTATCAGAACTA
TTACCACGGGAAAATATAAA

Downstream 100 bases:

>100_bases
ATGCATAATGTATTATTATAACAGTTCTGAAACTAAGATACATGTTTATTATATGGGGAGTAATTGAATACTGAATAATA
AACATGTAGTTATTATTTTT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MNYSALITLAQKLIQQPSVSPNHHSCHEIIANYLEKLNFNVELMRFDNTLNLWAFHSCKKQQKHTTLLFIGHTDVVDPGD
PQFWDYPPFSGLVHNNMLHGRGAIDMKGALAAMLVATANFIDQYPNYQGRIAFLITSDEEGSGINGTTKVVESLIARNEH
INYCIVGEPSSQDQLGDVIKNGRRGSLTGQLTIHGSQGHVAYPQFSKNPIHLIIPALSDLLNITWDREKSILFPPTAIQI
TNIYSNHKNNNVTPHTVILNFNVRFNDKCSIDNIKRYINDIFTRHTLPYNINWKLSAEPYFSKPGQLTNVAINAIKYYQK
FEPRLETTGGTSDGRFIAKMGTEVIELGARNHMIHKVNEYIDLIDLKLLSSIYKKIIEDLILIQ

Sequences:

>Translated_384_residues
MNYSALITLAQKLIQQPSVSPNHHSCHEIIANYLEKLNFNVELMRFDNTLNLWAFHSCKKQQKHTTLLFIGHTDVVDPGD
PQFWDYPPFSGLVHNNMLHGRGAIDMKGALAAMLVATANFIDQYPNYQGRIAFLITSDEEGSGINGTTKVVESLIARNEH
INYCIVGEPSSQDQLGDVIKNGRRGSLTGQLTIHGSQGHVAYPQFSKNPIHLIIPALSDLLNITWDREKSILFPPTAIQI
TNIYSNHKNNNVTPHTVILNFNVRFNDKCSIDNIKRYINDIFTRHTLPYNINWKLSAEPYFSKPGQLTNVAINAIKYYQK
FEPRLETTGGTSDGRFIAKMGTEVIELGARNHMIHKVNEYIDLIDLKLLSSIYKKIIEDLILIQ
>Mature_384_residues
MNYSALITLAQKLIQQPSVSPNHHSCHEIIANYLEKLNFNVELMRFDNTLNLWAFHSCKKQQKHTTLLFIGHTDVVDPGD
PQFWDYPPFSGLVHNNMLHGRGAIDMKGALAAMLVATANFIDQYPNYQGRIAFLITSDEEGSGINGTTKVVESLIARNEH
INYCIVGEPSSQDQLGDVIKNGRRGSLTGQLTIHGSQGHVAYPQFSKNPIHLIIPALSDLLNITWDREKSILFPPTAIQI
TNIYSNHKNNNVTPHTVILNFNVRFNDKCSIDNIKRYINDIFTRHTLPYNINWKLSAEPYFSKPGQLTNVAINAIKYYQK
FEPRLETTGGTSDGRFIAKMGTEVIELGARNHMIHKVNEYIDLIDLKLLSSIYKKIIEDLILIQ

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=376, Percent_Identity=48.936170212766, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI1790395, Length=315, Percent_Identity=24.1269841269841, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_BLOPB (Q492F7)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_278021.1
- ProteinModelPortal:   Q492F7
- STRING:   Q492F7
- MEROPS:   M20.010
- GeneID:   3562795
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_534
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- ProtClustDB:   PRK13009
- BioCyc:   CBLO291272:BPEN_534-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 43483; Mature: 43483

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 74-74 ACT_SITE 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYSALITLAQKLIQQPSVSPNHHSCHEIIANYLEKLNFNVELMRFDNTLNLWAFHSCKK
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEHHHHH
QQKHTTLLFIGHTDVVDPGDPQFWDYPPFSGLVHNNMLHGRGAIDMKGALAAMLVATANF
HCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHCCEECCCCCCCHHHHHHHHHHHHHHH
IDQYPNYQGRIAFLITSDEEGSGINGTTKVVESLIARNEHINYCIVGEPSSQDQLGDVIK
HHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHH
NGRRGSLTGQLTIHGSQGHVAYPQFSKNPIHLIIPALSDLLNITWDREKSILFPPTAIQI
CCCCCCEEEEEEEECCCCCEECCCCCCCCEEEEHHHHHHHHCCCCCCCCCEECCCCEEEE
TNIYSNHKNNNVTPHTVILNFNVRFNDKCSIDNIKRYINDIFTRHTLPYNINWKLSAEPY
EEEECCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCC
FSKPGQLTNVAINAIKYYQKFEPRLETTGGTSDGRFIAKMGTEVIELGARNHMIHKVNEY
CCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCEEHHHHCHHHHHHCCCCHHHHHHHHH
IDLIDLKLLSSIYKKIIEDLILIQ
HHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNYSALITLAQKLIQQPSVSPNHHSCHEIIANYLEKLNFNVELMRFDNTLNLWAFHSCKK
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEHHHHH
QQKHTTLLFIGHTDVVDPGDPQFWDYPPFSGLVHNNMLHGRGAIDMKGALAAMLVATANF
HCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHCCEECCCCCCCHHHHHHHHHHHHHHH
IDQYPNYQGRIAFLITSDEEGSGINGTTKVVESLIARNEHINYCIVGEPSSQDQLGDVIK
HHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHH
NGRRGSLTGQLTIHGSQGHVAYPQFSKNPIHLIIPALSDLLNITWDREKSILFPPTAIQI
CCCCCCEEEEEEEECCCCCEECCCCCCCCEEEEHHHHHHHHCCCCCCCCCEECCCCEEEE
TNIYSNHKNNNVTPHTVILNFNVRFNDKCSIDNIKRYINDIFTRHTLPYNINWKLSAEPY
EEEECCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCC
FSKPGQLTNVAINAIKYYQKFEPRLETTGGTSDGRFIAKMGTEVIELGARNHMIHKVNEY
CCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCEEHHHHCHHHHHHCCCCHHHHHHHHH
IDLIDLKLLSSIYKKIIEDLILIQ
HHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA