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The map label for this gene is metG

Identifier: 71892244

GI number: 71892244

Start: 579039

End: 580706

Strand: Direct

Name: metG

Synonym: BPEN_486

Alternate gene names: 71892244

Gene position: 579039-580706 (Clockwise)

Preceding gene: 71892242

Following gene: 71892246

Centisome position: 73.14

GC content: 31.83

Gene sequence:

>1668_bases
ATGACTACAAAAAAAATGCTAGTTACTTGTGCCTTGCCTTACGCTAATGGTTCACTTCATATTGGACACATGTTAGAACA
TATACAAGCAGATATCTGGGTTCGTTATCAACGCATGCAAGGAAATTGCGTATACTTTATTTGCGCAGATGATGCGCATG
GAACTGCCATTATGTTAAAATCACAACAACTTAATATAGCTCCAGAACAAATGATTGCCCAAATACGACAAGAACATCAA
CGAGATTGCTATAAATTCGGAATTAGCTATGATAACTACTATTCTACTCACAGTGACGAAACTCGTGAATTACTGCATGA
CATTTACTCTCGATTAAACACACGTGGATTCATCAAATCTAAATTTATTTCTCAATTATATGATTCTAAAAAAAATATGT
TTTTACCGGATCGATTTGTTAAAGGCATATGTCCAAAATGCAAAAAAGATGATCAATATGGAGATAATTGTGCAGCATGT
GGTACTATCTATACCTCTTTAGAGCTTATAAACCCCAAATCAGTTATTTCTGGAACGTCTCCGATAATACGCAAGTCTGA
ACATCTCTTTTTTGATTTACCAGCTTTTACTGATACATTGCGTACATGGATTCGATCAGGAAGCATACAAAAAGAAGTAG
CTAACAAAGTAGAAGAATGGTTTAAATTAGGTTTAAAAAAATGGGATATTTCTAGAGACGCTCCTTATTTTGGATTTAAA
ATTCCTAATTCTTCAGAAAAATATTTTTATGTATGGATGGATGCCCCTGTCGGATATATGGGTACCTTTAAAAACCTATG
CAAAAAAAACAAAAACATATCTTTTAACGACTTTTGGGGATCAAATTCCAAAACTGACTTATATCATTTCATAGGTAAAG
ATATTATTTACTTTCATTGTTTATTTTGGCCAGCTGTTTTGTCAGGTAGTCAATTTCGAAAACCAACTAATATATTTGTT
CATGGGCATGTTACTTTAAATGGATCTAAAATATCAAAATCTAAAGGGACTTGCATCAACGTAAGTACTTACTTATCATG
TTTAAACCCAGATTATTTAAGATATTATTATGCCACGAAGCTTTCATCTCATGCTAACGACATAGACTTGAATTTATCAG
ATTTTATTACTAGAGTAAATTCGGATATTATTAATAAAATATTAAATTTAGCATCTAGAAATTCTGGATTTATACATCAA
TATTATAATGGCCATTTGTCGAATACATTAACACATCCACTTATATATAATATGTTTATTGAATCCAGACATTATATAGG
AAAATTATTTCAAAAAAGAGAATTTAACTACGCAATGCGTGAAATTATGAAATTAGCAGATGAAGCTAATCGTTACATAG
ATAAACATGCTCCGTGGCATATCGCAAAAAAAATAGACCGTAGACAAGAGGCATTATCTATTTATTCTATGGGTATTCAG
TTGTTTCGTGTGCTGATGATCTATTTGAAACCTGTCTTACCTAAATTAGCTAATTATAGCGAATGTTTTCTCAACACTCG
TCTTACATGGGGCAGTCTTTCTGCGCCTTTATCAAATCATCGAATTAATAAATTCAAAATAATTTTTTCTCGAATCCATC
CCGATCAAATTGCATCTATGACAAATAAATCTCAATTACATGAGCGCCTTACGGATAATAATGTATGA

Upstream 100 bases:

>100_bases
TGTATATTCATGATGTAAATTATAAAAAACAATTACATTTTAACAATTACAGTATATGTCGTCATACGACATATTTAAAT
TTTAAATCCACAAAACTATT

Downstream 100 bases:

>100_bases
AGAGCACAAATCACACTCTTTAACAATAAACATAATATAACGTTAAAACAACTAACTGTATCATTGCATACGCTACACAA
TGGAAACTGTACAGGAATTG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MTTKKMLVTCALPYANGSLHIGHMLEHIQADIWVRYQRMQGNCVYFICADDAHGTAIMLKSQQLNIAPEQMIAQIRQEHQ
RDCYKFGISYDNYYSTHSDETRELLHDIYSRLNTRGFIKSKFISQLYDSKKNMFLPDRFVKGICPKCKKDDQYGDNCAAC
GTIYTSLELINPKSVISGTSPIIRKSEHLFFDLPAFTDTLRTWIRSGSIQKEVANKVEEWFKLGLKKWDISRDAPYFGFK
IPNSSEKYFYVWMDAPVGYMGTFKNLCKKNKNISFNDFWGSNSKTDLYHFIGKDIIYFHCLFWPAVLSGSQFRKPTNIFV
HGHVTLNGSKISKSKGTCINVSTYLSCLNPDYLRYYYATKLSSHANDIDLNLSDFITRVNSDIINKILNLASRNSGFIHQ
YYNGHLSNTLTHPLIYNMFIESRHYIGKLFQKREFNYAMREIMKLADEANRYIDKHAPWHIAKKIDRRQEALSIYSMGIQ
LFRVLMIYLKPVLPKLANYSECFLNTRLTWGSLSAPLSNHRINKFKIIFSRIHPDQIASMTNKSQLHERLTDNNV

Sequences:

>Translated_555_residues
MTTKKMLVTCALPYANGSLHIGHMLEHIQADIWVRYQRMQGNCVYFICADDAHGTAIMLKSQQLNIAPEQMIAQIRQEHQ
RDCYKFGISYDNYYSTHSDETRELLHDIYSRLNTRGFIKSKFISQLYDSKKNMFLPDRFVKGICPKCKKDDQYGDNCAAC
GTIYTSLELINPKSVISGTSPIIRKSEHLFFDLPAFTDTLRTWIRSGSIQKEVANKVEEWFKLGLKKWDISRDAPYFGFK
IPNSSEKYFYVWMDAPVGYMGTFKNLCKKNKNISFNDFWGSNSKTDLYHFIGKDIIYFHCLFWPAVLSGSQFRKPTNIFV
HGHVTLNGSKISKSKGTCINVSTYLSCLNPDYLRYYYATKLSSHANDIDLNLSDFITRVNSDIINKILNLASRNSGFIHQ
YYNGHLSNTLTHPLIYNMFIESRHYIGKLFQKREFNYAMREIMKLADEANRYIDKHAPWHIAKKIDRRQEALSIYSMGIQ
LFRVLMIYLKPVLPKLANYSECFLNTRLTWGSLSAPLSNHRINKFKIIFSRIHPDQIASMTNKSQLHERLTDNNV
>Mature_554_residues
TTKKMLVTCALPYANGSLHIGHMLEHIQADIWVRYQRMQGNCVYFICADDAHGTAIMLKSQQLNIAPEQMIAQIRQEHQR
DCYKFGISYDNYYSTHSDETRELLHDIYSRLNTRGFIKSKFISQLYDSKKNMFLPDRFVKGICPKCKKDDQYGDNCAACG
TIYTSLELINPKSVISGTSPIIRKSEHLFFDLPAFTDTLRTWIRSGSIQKEVANKVEEWFKLGLKKWDISRDAPYFGFKI
PNSSEKYFYVWMDAPVGYMGTFKNLCKKNKNISFNDFWGSNSKTDLYHFIGKDIIYFHCLFWPAVLSGSQFRKPTNIFVH
GHVTLNGSKISKSKGTCINVSTYLSCLNPDYLRYYYATKLSSHANDIDLNLSDFITRVNSDIINKILNLASRNSGFIHQY
YNGHLSNTLTHPLIYNMFIESRHYIGKLFQKREFNYAMREIMKLADEANRYIDKHAPWHIAKKIDRRQEALSIYSMGIQL
FRVLMIYLKPVLPKLANYSECFLNTRLTWGSLSAPLSNHRINKFKIIFSRIHPDQIASMTNKSQLHERLTDNNV

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=563, Percent_Identity=26.6429840142096, Blast_Score=240, Evalue=3e-63,
Organism=Homo sapiens, GI24308436, Length=521, Percent_Identity=24.3761996161228, Blast_Score=149, Evalue=9e-36,
Organism=Escherichia coli, GI1788432, Length=545, Percent_Identity=61.4678899082569, Blast_Score=756, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=574, Percent_Identity=25.7839721254355, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI212645096, Length=517, Percent_Identity=23.7911025145068, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI71992781, Length=337, Percent_Identity=25.5192878338279, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321704, Length=563, Percent_Identity=27.1758436944938, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6321610, Length=545, Percent_Identity=22.5688073394495, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24655311, Length=563, Percent_Identity=27.5310834813499, Blast_Score=228, Evalue=1e-59,
Organism=Drosophila melanogaster, GI21358483, Length=521, Percent_Identity=25.7197696737044, Blast_Score=133, Evalue=4e-31,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_BLOPB (Q492J8)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277978.1
- ProteinModelPortal:   Q492J8
- SMR:   Q492J8
- STRING:   Q492J8
- GeneID:   3562993
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_486
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   CGNQLDP
- ProtClustDB:   PRK12268
- BioCyc:   CBLO291272:BPEN_486-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 64542; Mature: 64411

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 333-333

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKKMLVTCALPYANGSLHIGHMLEHIQADIWVRYQRMQGNCVYFICADDAHGTAIMLK
CCCCEEEEEEEEECCCCCEEHHHHHHHHHHHHHEEEEEECCCEEEEEECCCCCCCEEEEE
SQQLNIAPEQMIAQIRQEHQRDCYKFGISYDNYYSTHSDETRELLHDIYSRLNTRGFIKS
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
KFISQLYDSKKNMFLPDRFVKGICPKCKKDDQYGDNCAACGTIYTSLELINPKSVISGTS
HHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHEECCCHHHHCCCC
PIIRKSEHLFFDLPAFTDTLRTWIRSGSIQKEVANKVEEWFKLGLKKWDISRDAPYFGFK
HHHCCCCCEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEE
IPNSSEKYFYVWMDAPVGYMGTFKNLCKKNKNISFNDFWGSNSKTDLYHFIGKDIIYFHC
CCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHH
LFWPAVLSGSQFRKPTNIFVHGHVTLNGSKISKSKGTCINVSTYLSCLNPDYLRYYYATK
HHHHHHHCCCCCCCCCEEEEEEEEEECCCEECCCCCCEEEHHHHHHHCCCHHHHHHHHHH
LSSHANDIDLNLSDFITRVNSDIINKILNLASRNSGFIHQYYNGHLSNTLTHPLIYNMFI
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHCCHHHHHHHHHHHHHHHH
ESRHYIGKLFQKREFNYAMREIMKLADEANRYIDKHAPWHIAKKIDRRQEALSIYSMGIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LFRVLMIYLKPVLPKLANYSECFLNTRLTWGSLSAPLSNHRINKFKIIFSRIHPDQIASM
HHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCHHEEEEHHHCCHHHHHHH
TNKSQLHERLTDNNV
HHHHHHHHHHCCCCC
>Mature Secondary Structure 
TTKKMLVTCALPYANGSLHIGHMLEHIQADIWVRYQRMQGNCVYFICADDAHGTAIMLK
CCCEEEEEEEEECCCCCEEHHHHHHHHHHHHHEEEEEECCCEEEEEECCCCCCCEEEEE
SQQLNIAPEQMIAQIRQEHQRDCYKFGISYDNYYSTHSDETRELLHDIYSRLNTRGFIKS
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
KFISQLYDSKKNMFLPDRFVKGICPKCKKDDQYGDNCAACGTIYTSLELINPKSVISGTS
HHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHEECCCHHHHCCCC
PIIRKSEHLFFDLPAFTDTLRTWIRSGSIQKEVANKVEEWFKLGLKKWDISRDAPYFGFK
HHHCCCCCEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEE
IPNSSEKYFYVWMDAPVGYMGTFKNLCKKNKNISFNDFWGSNSKTDLYHFIGKDIIYFHC
CCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHH
LFWPAVLSGSQFRKPTNIFVHGHVTLNGSKISKSKGTCINVSTYLSCLNPDYLRYYYATK
HHHHHHHCCCCCCCCCEEEEEEEEEECCCEECCCCCCEEEHHHHHHHCCCHHHHHHHHHH
LSSHANDIDLNLSDFITRVNSDIINKILNLASRNSGFIHQYYNGHLSNTLTHPLIYNMFI
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHCCHHHHHHHHHHHHHHHH
ESRHYIGKLFQKREFNYAMREIMKLADEANRYIDKHAPWHIAKKIDRRQEALSIYSMGIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LFRVLMIYLKPVLPKLANYSECFLNTRLTWGSLSAPLSNHRINKFKIIFSRIHPDQIASM
HHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCHHEEEEHHHCCHHHHHHH
TNKSQLHERLTDNNV
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA