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The map label for this gene is manX [H]

Identifier: 71892221

GI number: 71892221

Start: 546359

End: 547342

Strand: Direct

Name: manX [H]

Synonym: BPEN_459

Alternate gene names: 71892221

Gene position: 546359-547342 (Clockwise)

Preceding gene: 71892219

Following gene: 71892222

Centisome position: 69.01

GC content: 31.91

Gene sequence:

>984_bases
GTGACTATTGCTATTATATTAGGAGCTCATGGTTCTGTATCTAAAAAATTGATTGAAACCACTGAAATGATCATAGGAAA
ACAACAAAATGTTGCATGGGTTGATCTATTACCCGAAGAAAATACTAAAATATTAATTGAAAAATATCATGCACATCTAT
CAAATCTTAATAAAAGCGCAGGGGTATTATTTTTGGTGGACACCTGGGGAGGCAGCCCGTTTAATGCCGCTCATAGCATC
GTGTTAAATAAAGAAAAATACGATATCGTTACAGGAATGAATATCCCTATGTTAATAGAAGTTTTCATGGCACGCGACAA
TATCCATTCCTTTCAAGAGCTAATACAAATAGCATTAAAATCTGGTCATGAAAGTATAAAATCAGTAAATTACTCCCTAA
CTCACACCCATTTATACAATGAAAAAGACATTACTAAACACGCCACCGTACCTAAACCTAATAAACTATCAGATAAAAAT
AACAAAAACCATATGATCATTTGCTTAGCACGTATTGACGATAGATTGATTCATGGACAGGTAGTAACTCGTTGGGCTAA
AGAATACAATATTAAACGCATCATAGTAGTAAACGACGAAGTATCTAAAGATATGATCCGAAAAACTTTACTTACTCAAG
TAACTCCCCCCGGAATAACTGCTCATGTTCTAGATATCAATAAAACTATACGTGTATACAATAATCCTAAATATGCCGGA
GATAAAGTGATAATGTTATTCACTAATCCTACAGATGTTTTACGTCTTTTTGAGGGCGGCATTCCTATCAATTCAGTAAA
TATTGGAGGAATGGCTTTTTATAAAGATAAAAAACAAATAAATAATGCTATATCAGTAAATAACACAGATATTGAAGCTT
TTAAAAAACTCGATCAATATGGCATTGAATTAGAAGTACGTAAAGTGCCATCAGATCCATCATTAAAAATGATGCAATTA
ATAAAAAAAATAAATAATTCTTAA

Upstream 100 bases:

>100_bases
AATGTTAACCTATATAGGATAACATTAAAAACAATTCCTTGTTATTTTAAGAATTAAAATAACAAGGAATTGCTAAAAAC
ATACAAAAGAGGAAAAAGTA

Downstream 100 bases:

>100_bases
TTAATTAGATGTACAATTTTATTTAATAAATTCATGTAAACGCTATATTTTATTTTCATATATTTAGGAAATGAAATGGA
AATTAACACACTTCAAATTA

Product: PTS family enzyme IIA/enzyme IIB, mannose-specific

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTIAIILGAHGSVSKKLIETTEMIIGKQQNVAWVDLLPEENTKILIEKYHAHLSNLNKSAGVLFLVDTWGGSPFNAAHSI
VLNKEKYDIVTGMNIPMLIEVFMARDNIHSFQELIQIALKSGHESIKSVNYSLTHTHLYNEKDITKHATVPKPNKLSDKN
NKNHMIICLARIDDRLIHGQVVTRWAKEYNIKRIIVVNDEVSKDMIRKTLLTQVTPPGITAHVLDINKTIRVYNNPKYAG
DKVIMLFTNPTDVLRLFEGGIPINSVNIGGMAFYKDKKQINNAISVNNTDIEAFKKLDQYGIELEVRKVPSDPSLKMMQL
IKKINNS

Sequences:

>Translated_327_residues
MTIAIILGAHGSVSKKLIETTEMIIGKQQNVAWVDLLPEENTKILIEKYHAHLSNLNKSAGVLFLVDTWGGSPFNAAHSI
VLNKEKYDIVTGMNIPMLIEVFMARDNIHSFQELIQIALKSGHESIKSVNYSLTHTHLYNEKDITKHATVPKPNKLSDKN
NKNHMIICLARIDDRLIHGQVVTRWAKEYNIKRIIVVNDEVSKDMIRKTLLTQVTPPGITAHVLDINKTIRVYNNPKYAG
DKVIMLFTNPTDVLRLFEGGIPINSVNIGGMAFYKDKKQINNAISVNNTDIEAFKKLDQYGIELEVRKVPSDPSLKMMQL
IKKINNS
>Mature_326_residues
TIAIILGAHGSVSKKLIETTEMIIGKQQNVAWVDLLPEENTKILIEKYHAHLSNLNKSAGVLFLVDTWGGSPFNAAHSIV
LNKEKYDIVTGMNIPMLIEVFMARDNIHSFQELIQIALKSGHESIKSVNYSLTHTHLYNEKDITKHATVPKPNKLSDKNN
KNHMIICLARIDDRLIHGQVVTRWAKEYNIKRIIVVNDEVSKDMIRKTLLTQVTPPGITAHVLDINKTIRVYNNPKYAGD
KVIMLFTNPTDVLRLFEGGIPINSVNIGGMAFYKDKKQINNAISVNNTDIEAFKKLDQYGIELEVRKVPSDPSLKMMQLI
KKINNS

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=325, Percent_Identity=57.2307692307692, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI87082217, Length=157, Percent_Identity=36.3057324840764, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789527, Length=153, Percent_Identity=33.9869281045752, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 36916; Mature: 36784

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS51096 PTS_EIIA_TYPE_4 ; PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAIILGAHGSVSKKLIETTEMIIGKQQNVAWVDLLPEENTKILIEKYHAHLSNLNKSA
CEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEHHHHHHHHHCCCCCC
GVLFLVDTWGGSPFNAAHSIVLNKEKYDIVTGMNIPMLIEVFMARDNIHSFQELIQIALK
CEEEEEECCCCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHH
SGHESIKSVNYSLTHTHLYNEKDITKHATVPKPNKLSDKNNKNHMIICLARIDDRLIHGQ
CCHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHH
VVTRWAKEYNIKRIIVVNDEVSKDMIRKTLLTQVTPPGITAHVLDINKTIRVYNNPKYAG
HHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCC
DKVIMLFTNPTDVLRLFEGGIPINSVNIGGMAFYKDKKQINNAISVNNTDIEAFKKLDQY
CEEEEEEECCHHHHHHHHCCCCEEEEECCCEEEECCHHHHCCEEECCCCCHHHHHHHHHC
GIELEVRKVPSDPSLKMMQLIKKINNS
CCEEEEEECCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TIAIILGAHGSVSKKLIETTEMIIGKQQNVAWVDLLPEENTKILIEKYHAHLSNLNKSA
EEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEHHHHHHHHHCCCCCC
GVLFLVDTWGGSPFNAAHSIVLNKEKYDIVTGMNIPMLIEVFMARDNIHSFQELIQIALK
CEEEEEECCCCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHH
SGHESIKSVNYSLTHTHLYNEKDITKHATVPKPNKLSDKNNKNHMIICLARIDDRLIHGQ
CCHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHH
VVTRWAKEYNIKRIIVVNDEVSKDMIRKTLLTQVTPPGITAHVLDINKTIRVYNNPKYAG
HHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCC
DKVIMLFTNPTDVLRLFEGGIPINSVNIGGMAFYKDKKQINNAISVNNTDIEAFKKLDQY
CEEEEEEECCHHHHHHHHCCCCEEEEECCCEEEECCHHHHCCEEECCCCCHHHHHHHHHC
GIELEVRKVPSDPSLKMMQLIKKINNS
CCEEEEEECCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]