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The map label for this gene is trpA [H]

Identifier: 71892205

GI number: 71892205

Start: 524616

End: 525446

Strand: Direct

Name: trpA [H]

Synonym: BPEN_443

Alternate gene names: 71892205

Gene position: 524616-525446 (Clockwise)

Preceding gene: 71892204

Following gene: 71892206

Centisome position: 66.27

GC content: 30.93

Gene sequence:

>831_bases
ATGAATCGTTACAAAAAATTATTTACACATTTAAAGAAAAAAAAAATAGGCGCATTTGTTCCTTTTATTGCTGTAGGATA
TCCCGATCCTATTTCTTTTATGCATATCATCGATACATTAGTGTCTTCAGGTTCCGATGCACTAGAATTAGGAATACCAT
TTTCTGATCCTATATCAGATGGACCTATAATACAAAAAAGTATGGAACGTGCTTTCAAATCAGGTATAAATTTTTCACGT
TGTGTGACAATACTACACAATATTCGCAACAAATATCCAAATTTGCCCATCGGATTACTAATTTATGCAAATTTAGTCTT
TAAAAATGGAATAGATAATTTCTATTCATGTTGCTCAAAACTAGATATAGATTCTATATTAATTCCTGATCTCCCTGTAG
AAGAAAGTCTACCGTTTTATAATGCTGCTATACAACATAAAATAGCCCACATCTTCATTTGTCCACCTAATGCTACCGAA
GAATTAATCCATAATATTACTACTCTCGGACATGGATACATCTATTTATTATCCCGACCCGGAGTAACTGGCATTGATAT
TAATGCAAAAAAAATTTATAATGCTGTATTACATAGTATAATTATGTGTATCCAAAAACAAAAAAAAAAATTACCTATAC
TACAAGGTTTTGGAATACACACACCGCTTCAAGCTCAAGAATCGTTATTGTTAGGAACTTCTGGGATCATTACCGGTTCT
AAAATTGCAAGCATTATAGAAGAAAATATATCTAACACAAAACTAGCATTTAAGAAAATAGAAAAATTAGTACGTCTTAT
GAAGACATCCATGAGTTCTAATACTCATTAG

Upstream 100 bases:

>100_bases
CAGATAAAGCACAAATTTTAGTAGTTAATTTGTCTGGACGTGGAGATAAAGACATTTCTACCGTTCATAATGCATTACAA
AAAGAAAGAGATAAATAACA

Downstream 100 bases:

>100_bases
AATTAATAATAATAGGTATTTTTCTATTCTTATTTTTAATAAAAAACCGAACGATTATATATTATAATCATCAAAATTAG
CACGCACATCCTTACAAAAA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MNRYKKLFTHLKKKKIGAFVPFIAVGYPDPISFMHIIDTLVSSGSDALELGIPFSDPISDGPIIQKSMERAFKSGINFSR
CVTILHNIRNKYPNLPIGLLIYANLVFKNGIDNFYSCCSKLDIDSILIPDLPVEESLPFYNAAIQHKIAHIFICPPNATE
ELIHNITTLGHGYIYLLSRPGVTGIDINAKKIYNAVLHSIIMCIQKQKKKLPILQGFGIHTPLQAQESLLLGTSGIITGS
KIASIIEENISNTKLAFKKIEKLVRLMKTSMSSNTH

Sequences:

>Translated_276_residues
MNRYKKLFTHLKKKKIGAFVPFIAVGYPDPISFMHIIDTLVSSGSDALELGIPFSDPISDGPIIQKSMERAFKSGINFSR
CVTILHNIRNKYPNLPIGLLIYANLVFKNGIDNFYSCCSKLDIDSILIPDLPVEESLPFYNAAIQHKIAHIFICPPNATE
ELIHNITTLGHGYIYLLSRPGVTGIDINAKKIYNAVLHSIIMCIQKQKKKLPILQGFGIHTPLQAQESLLLGTSGIITGS
KIASIIEENISNTKLAFKKIEKLVRLMKTSMSSNTH
>Mature_276_residues
MNRYKKLFTHLKKKKIGAFVPFIAVGYPDPISFMHIIDTLVSSGSDALELGIPFSDPISDGPIIQKSMERAFKSGINFSR
CVTILHNIRNKYPNLPIGLLIYANLVFKNGIDNFYSCCSKLDIDSILIPDLPVEESLPFYNAAIQHKIAHIFICPPNATE
ELIHNITTLGHGYIYLLSRPGVTGIDINAKKIYNAVLHSIIMCIQKQKKKLPILQGFGIHTPLQAQESLLLGTSGIITGS
KIASIIEENISNTKLAFKKIEKLVRLMKTSMSSNTH

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=273, Percent_Identity=47.6190476190476, Blast_Score=261, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6321412, Length=244, Percent_Identity=29.9180327868852, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 30676; Mature: 30676

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA ; PS00327 BACTERIAL_OPSIN_RET

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRYKKLFTHLKKKKIGAFVPFIAVGYPDPISFMHIIDTLVSSGSDALELGIPFSDPISD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
GPIIQKSMERAFKSGINFSRCVTILHNIRNKYPNLPIGLLIYANLVFKNGIDNFYSCCSK
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHH
LDIDSILIPDLPVEESLPFYNAAIQHKIAHIFICPPNATEELIHNITTLGHGYIYLLSRP
CCCCCEECCCCCCCCCCCHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECC
GVTGIDINAKKIYNAVLHSIIMCIQKQKKKLPILQGFGIHTPLQAQESLLLGTSGIITGS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHH
KIASIIEENISNTKLAFKKIEKLVRLMKTSMSSNTH
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNRYKKLFTHLKKKKIGAFVPFIAVGYPDPISFMHIIDTLVSSGSDALELGIPFSDPISD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
GPIIQKSMERAFKSGINFSRCVTILHNIRNKYPNLPIGLLIYANLVFKNGIDNFYSCCSK
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHH
LDIDSILIPDLPVEESLPFYNAAIQHKIAHIFICPPNATEELIHNITTLGHGYIYLLSRP
CCCCCEECCCCCCCCCCCHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECC
GVTGIDINAKKIYNAVLHSIIMCIQKQKKKLPILQGFGIHTPLQAQESLLLGTSGIITGS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHH
KIASIIEENISNTKLAFKKIEKLVRLMKTSMSSNTH
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12886019 [H]