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The map label for this gene is tyrS

Identifier: 71892149

GI number: 71892149

Start: 448260

End: 449543

Strand: Direct

Name: tyrS

Synonym: BPEN_382

Alternate gene names: 71892149

Gene position: 448260-449543 (Clockwise)

Preceding gene: 71892148

Following gene: 71892152

Centisome position: 56.62

GC content: 30.14

Gene sequence:

>1284_bases
ATGAATAATAAAAATATTGATATAATACAACTTTTATACGAACGTGATTTAATAGCACAAATTACAAACCAAGAAGCATT
AATTAAAATATTAAAATCAAAATCCATTACTTTATATTGCGGATTTGATCCGACATCTGACAGCTTACATATAGGACACT
TAGTTCCACTGTTATGCTTAAGACAATTCCAAATATTTGGACATCGTCCTATAATTTTGCTAGGAGGGGGTACTGGATTG
ATTGGAGACCCAAGTTTTAAAGATTCCGAACGAAAACTCAATTTAATTGAAACAGTACAAAAATGGATAGAAAAAATCAA
ATACCAAGTATCTTTATTCGTAGATTTTTCTCATAGTCAATATTCGCAAGCCTGTATCGTTAACAATTACGATTGGCTAT
CTTCTATATCACTCTTAACATTTTTAAGAGATATTGGTAAATTTTTTTCTGTAAATAAAATGATAAATAAGGATGCTATA
AAAAAACGGTTACAAAAAAATAATTATGGAATTTCTTATACTGAATTTTCCTATAATTTATTACAAAGTTATGATTTTAC
CTATTTACATAAAAATTATAACGCAATACTGCAAATCGGTGGTTCTGATCAATGGGGCAATATTATATCAGGTATTGATC
TGATACATCGCATATATAAAAGAACTACTTACGGGCTCACTATGCCTTTACTGACCAAATCTGATAACACTAAATTTGGA
AAAACAGAAAAACATACTATATGGTTAGATGCAAAAAAAACCAGCCCTTATACATTCTATCAATATTGGATCAATACTTC
TGATGAAGAAGTATATCGTTTTCTTAAATTTTTTACCAACATCGATGTAGATGCGATCAATACCTTAAAAAAAGAAGATA
AAATGAAGAATACAAAACCACAAGCTCAAATTATATTAGCAGAGGAAATTACTCGATTAGTTCATGGAAAATCAGGATTA
AAAACAGCTCAAAAAATTACCAGAAATCTTTTTACAGGACAAATACATAAACTTACCTTAGATGATTTCGAACAGCTATT
TCAAGATGGCATACCGACCGTACCACTGAAAGCAGGTATTACATTACAACAAGCGTTGGTAGAATCTAAATTAGTAGTGT
CTCGTGCACAAGCACGAGAATTAATTTCATCTAATAGCATCACTGTAAACTCAAAAAAACAATTGAAAACAGAATATATT
TTTTGTGCCACCGACAGATTATACAATCGATTTACACTATTAAGACGCGGAAAAAAACACCACTGCTTAATCACATGGGA
ATGA

Upstream 100 bases:

>100_bases
TTATGATATATAAAAAATATAACTAAACTTAATAAAGTAGTATTGACTTTTATAAAAGTCATATAAATACTCTTGATCGC
TGTACTAAAACTATATTTGT

Downstream 100 bases:

>100_bases
TCTAGTTTAGATAATATATTGTTTTATGTATATAACTATTTATATTGTTTGAAAATATAAATACAATTTCCCGCAATACC
TTACTACGCCATGCGTAGTA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNNKNIDIIQLLYERDLIAQITNQEALIKILKSKSITLYCGFDPTSDSLHIGHLVPLLCLRQFQIFGHRPIILLGGGTGL
IGDPSFKDSERKLNLIETVQKWIEKIKYQVSLFVDFSHSQYSQACIVNNYDWLSSISLLTFLRDIGKFFSVNKMINKDAI
KKRLQKNNYGISYTEFSYNLLQSYDFTYLHKNYNAILQIGGSDQWGNIISGIDLIHRIYKRTTYGLTMPLLTKSDNTKFG
KTEKHTIWLDAKKTSPYTFYQYWINTSDEEVYRFLKFFTNIDVDAINTLKKEDKMKNTKPQAQIILAEEITRLVHGKSGL
KTAQKITRNLFTGQIHKLTLDDFEQLFQDGIPTVPLKAGITLQQALVESKLVVSRAQARELISSNSITVNSKKQLKTEYI
FCATDRLYNRFTLLRRGKKHHCLITWE

Sequences:

>Translated_427_residues
MNNKNIDIIQLLYERDLIAQITNQEALIKILKSKSITLYCGFDPTSDSLHIGHLVPLLCLRQFQIFGHRPIILLGGGTGL
IGDPSFKDSERKLNLIETVQKWIEKIKYQVSLFVDFSHSQYSQACIVNNYDWLSSISLLTFLRDIGKFFSVNKMINKDAI
KKRLQKNNYGISYTEFSYNLLQSYDFTYLHKNYNAILQIGGSDQWGNIISGIDLIHRIYKRTTYGLTMPLLTKSDNTKFG
KTEKHTIWLDAKKTSPYTFYQYWINTSDEEVYRFLKFFTNIDVDAINTLKKEDKMKNTKPQAQIILAEEITRLVHGKSGL
KTAQKITRNLFTGQIHKLTLDDFEQLFQDGIPTVPLKAGITLQQALVESKLVVSRAQARELISSNSITVNSKKQLKTEYI
FCATDRLYNRFTLLRRGKKHHCLITWE
>Mature_427_residues
MNNKNIDIIQLLYERDLIAQITNQEALIKILKSKSITLYCGFDPTSDSLHIGHLVPLLCLRQFQIFGHRPIILLGGGTGL
IGDPSFKDSERKLNLIETVQKWIEKIKYQVSLFVDFSHSQYSQACIVNNYDWLSSISLLTFLRDIGKFFSVNKMINKDAI
KKRLQKNNYGISYTEFSYNLLQSYDFTYLHKNYNAILQIGGSDQWGNIISGIDLIHRIYKRTTYGLTMPLLTKSDNTKFG
KTEKHTIWLDAKKTSPYTFYQYWINTSDEEVYRFLKFFTNIDVDAINTLKKEDKMKNTKPQAQIILAEEITRLVHGKSGL
KTAQKITRNLFTGQIHKLTLDDFEQLFQDGIPTVPLKAGITLQQALVESKLVVSRAQARELISSNSITVNSKKQLKTEYI
FCATDRLYNRFTLLRRGKKHHCLITWE

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=403, Percent_Identity=31.5136476426799, Blast_Score=217, Evalue=1e-56,
Organism=Escherichia coli, GI1787925, Length=421, Percent_Identity=59.8574821852732, Blast_Score=554, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI25145802, Length=426, Percent_Identity=34.7417840375587, Blast_Score=214, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI25145805, Length=330, Percent_Identity=33.3333333333333, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6325160, Length=452, Percent_Identity=31.6371681415929, Blast_Score=231, Evalue=1e-61,
Organism=Drosophila melanogaster, GI20130383, Length=408, Percent_Identity=37.2549019607843, Blast_Score=280, Evalue=2e-75,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_BLOPB (Q492T7)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277881.1
- ProteinModelPortal:   Q492T7
- SMR:   Q492T7
- STRING:   Q492T7
- GeneID:   3562647
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_382
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   TFYIGFD
- ProtClustDB:   PRK05912
- BioCyc:   CBLO291272:BPEN_382-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 49396; Mature: 49396

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 39-39 BINDING 178-178 BINDING 182-182 BINDING 241-241

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKNIDIIQLLYERDLIAQITNQEALIKILKSKSITLYCGFDPTSDSLHIGHLVPLLCL
CCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCCCCEEHHHHHHHHHH
RQFQIFGHRPIILLGGGTGLIGDPSFKDSERKLNLIETVQKWIEKIKYQVSLFVDFSHSQ
HHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCC
YSQACIVNNYDWLSSISLLTFLRDIGKFFSVNKMINKDAIKKRLQKNNYGISYTEFSYNL
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHH
LQSYDFTYLHKNYNAILQIGGSDQWGNIISGIDLIHRIYKRTTYGLTMPLLTKSDNTKFG
HHHCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
KTEKHTIWLDAKKTSPYTFYQYWINTSDEEVYRFLKFFTNIDVDAINTLKKEDKMKNTKP
CCCCEEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
QAQIILAEEITRLVHGKSGLKTAQKITRNLFTGQIHKLTLDDFEQLFQDGIPTVPLKAGI
HHEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCCCCCCC
TLQQALVESKLVVSRAQARELISSNSITVNSKKQLKTEYIFCATDRLYNRFTLLRRGKKH
HHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHCEEEEEEEHHHHHHHHHHHHCCCCC
HCLITWE
EEEEEEC
>Mature Secondary Structure
MNNKNIDIIQLLYERDLIAQITNQEALIKILKSKSITLYCGFDPTSDSLHIGHLVPLLCL
CCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCCCCEEHHHHHHHHHH
RQFQIFGHRPIILLGGGTGLIGDPSFKDSERKLNLIETVQKWIEKIKYQVSLFVDFSHSQ
HHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCC
YSQACIVNNYDWLSSISLLTFLRDIGKFFSVNKMINKDAIKKRLQKNNYGISYTEFSYNL
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHH
LQSYDFTYLHKNYNAILQIGGSDQWGNIISGIDLIHRIYKRTTYGLTMPLLTKSDNTKFG
HHHCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
KTEKHTIWLDAKKTSPYTFYQYWINTSDEEVYRFLKFFTNIDVDAINTLKKEDKMKNTKP
CCCCEEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
QAQIILAEEITRLVHGKSGLKTAQKITRNLFTGQIHKLTLDDFEQLFQDGIPTVPLKAGI
HHEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCCCCCCC
TLQQALVESKLVVSRAQARELISSNSITVNSKKQLKTEYIFCATDRLYNRFTLLRRGKKH
HHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHCEEEEEEEHHHHHHHHHHHHCCCCC
HCLITWE
EEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA