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The map label for this gene is holA [H]

Identifier: 71892092

GI number: 71892092

Start: 378161

End: 379189

Strand: Reverse

Name: holA [H]

Synonym: BPEN_319

Alternate gene names: 71892092

Gene position: 379189-378161 (Counterclockwise)

Preceding gene: 71892093

Following gene: 71892091

Centisome position: 47.9

GC content: 25.85

Gene sequence:

>1029_bases
ATGATTTGGATATACCCAGAACAATTGTGTATAGAATTGAAAAAGGAATTGAGATCTGTTTATGTATTATTAGGGAATGA
TTCTTATTTTTTAGGAGATAGTTATCTAAATATTGTTAATACAGCCAGAATGCTAAATTTCAATGAATCTGTTAATATAG
CATTGGATATGTATTCTGATTGGGAGAATATATGTAATTTGTTCAGAGTATCAAGTTTATTTGAAAAACGCAGAATATTA
TCACTGAAATTTCCTCAAAATTATCCTGTTGCTATTTTAAAAAAAAAAGTGCCTTTATTGTTCTCATTTCTTCATGATGA
TTTAGTATTAATTTTATATATTCATGAATCGAATCAAATTAATAAAAATAATATTTGGTTACAATGTTTTAAAAAAATAG
GAATATTCGTTGATTGCAGCACTCCTACGCATACACGCTTGATAATGTGGATAGAAAATCAAGCTAAAAATATGAAACTT
GTTATAGAAAATTTAGCTTGTCAATTATTATGTTATTATTATGAAGGTAATTTAACATTATTAAATCAAACGTTGCGGAA
TCTGTCTTTAATCTATCCTGATGGAAATTTAAATTTTATTCGTGTAAAAAAAATAGTAACTGATTCAGCATGTTTTAATA
TGAATCATTGGATAGAATCGATTTTAACGGGCAACAAACAACGTGCTGATCGTATTCTAAAAAAATTGGAATATATAGGT
GTCGATTTAGAACTATTATTATGCAGGATTAAATCTGAAATACTGATGATAACTAACATGAAATACAATATGGTGCAAGG
AAAATCATTATTTAATTTGTTAAAAAAATATAAAATATATAGAAAGTATCATTGTATGTTGTTAGCTAGAGCTGTTAATC
GGTTAAGTTTATACCAATTATATCAAGCAATAGAATTATTAGCGCAAATAGAATTAATATATAAAAAGGATTATATTGTT
TTGTCACGATCAAGTTTTGAACTATTATCACTCATCTTATGTTACGATAAGAAGGTTTCGCTTAAATAG

Upstream 100 bases:

>100_bases
ATCAATTATTTCTGCGATGTGAAGATTTTAGTTAGGATATGTTGTAAAATCATTTTTTATATTTAATATAAATTTTTTAT
TTCACCATCAAAAATATATT

Downstream 100 bases:

>100_bases
CTATTTTAATCCAAATACATTAACCATATTTTATAATAGAATTTTTATAAATCTTATTTATTAGATGATTTGAGATAATT
ATTTATTTAAAAATAAATGC

Product: DNA polymerase III, delta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MIWIYPEQLCIELKKELRSVYVLLGNDSYFLGDSYLNIVNTARMLNFNESVNIALDMYSDWENICNLFRVSSLFEKRRIL
SLKFPQNYPVAILKKKVPLLFSFLHDDLVLILYIHESNQINKNNIWLQCFKKIGIFVDCSTPTHTRLIMWIENQAKNMKL
VIENLACQLLCYYYEGNLTLLNQTLRNLSLIYPDGNLNFIRVKKIVTDSACFNMNHWIESILTGNKQRADRILKKLEYIG
VDLELLLCRIKSEILMITNMKYNMVQGKSLFNLLKKYKIYRKYHCMLLARAVNRLSLYQLYQAIELLAQIELIYKKDYIV
LSRSSFELLSLILCYDKKVSLK

Sequences:

>Translated_342_residues
MIWIYPEQLCIELKKELRSVYVLLGNDSYFLGDSYLNIVNTARMLNFNESVNIALDMYSDWENICNLFRVSSLFEKRRIL
SLKFPQNYPVAILKKKVPLLFSFLHDDLVLILYIHESNQINKNNIWLQCFKKIGIFVDCSTPTHTRLIMWIENQAKNMKL
VIENLACQLLCYYYEGNLTLLNQTLRNLSLIYPDGNLNFIRVKKIVTDSACFNMNHWIESILTGNKQRADRILKKLEYIG
VDLELLLCRIKSEILMITNMKYNMVQGKSLFNLLKKYKIYRKYHCMLLARAVNRLSLYQLYQAIELLAQIELIYKKDYIV
LSRSSFELLSLILCYDKKVSLK
>Mature_342_residues
MIWIYPEQLCIELKKELRSVYVLLGNDSYFLGDSYLNIVNTARMLNFNESVNIALDMYSDWENICNLFRVSSLFEKRRIL
SLKFPQNYPVAILKKKVPLLFSFLHDDLVLILYIHESNQINKNNIWLQCFKKIGIFVDCSTPTHTRLIMWIENQAKNMKL
VIENLACQLLCYYYEGNLTLLNQTLRNLSLIYPDGNLNFIRVKKIVTDSACFNMNHWIESILTGNKQRADRILKKLEYIG
VDLELLLCRIKSEILMITNMKYNMVQGKSLFNLLKKYKIYRKYHCMLLARAVNRLSLYQLYQAIELLAQIELIYKKDYIV
LSRSSFELLSLILCYDKKVSLK

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the b

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786859, Length=335, Percent_Identity=36.7164179104478, Blast_Score=211, Evalue=6e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790 [H]

Pfam domain/function: PF06144 DNA_pol3_delta [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40432; Mature: 40432

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWIYPEQLCIELKKELRSVYVLLGNDSYFLGDSYLNIVNTARMLNFNESVNIALDMYSD
CEEECHHHHHHHHHHHHHEEEEEECCCCEEECCHHHHHHHHHHHEECCCCEEEEEEECCC
WENICNLFRVSSLFEKRRILSLKFPQNYPVAILKKKVPLLFSFLHDDLVLILYIHESNQI
HHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
NKNNIWLQCFKKIGIFVDCSTPTHTRLIMWIENQAKNMKLVIENLACQLLCYYYEGNLTL
CCCCHHHHHHHHCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHEEEEEEECCEEE
LNQTLRNLSLIYPDGNLNFIRVKKIVTDSACFNMNHWIESILTGNKQRADRILKKLEYIG
EHHHHHHCEEEEECCCEEEEEEEEEHHCHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHCC
VDLELLLCRIKSEILMITNMKYNMVQGKSLFNLLKKYKIYRKYHCMLLARAVNRLSLYQL
CCHHHHHHHHCCCEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQAIELLAQIELIYKKDYIVLSRSSFELLSLILCYDKKVSLK
HHHHHHHHHHHHHEECCEEEEECCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIWIYPEQLCIELKKELRSVYVLLGNDSYFLGDSYLNIVNTARMLNFNESVNIALDMYSD
CEEECHHHHHHHHHHHHHEEEEEECCCCEEECCHHHHHHHHHHHEECCCCEEEEEEECCC
WENICNLFRVSSLFEKRRILSLKFPQNYPVAILKKKVPLLFSFLHDDLVLILYIHESNQI
HHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
NKNNIWLQCFKKIGIFVDCSTPTHTRLIMWIENQAKNMKLVIENLACQLLCYYYEGNLTL
CCCCHHHHHHHHCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHEEEEEEECCEEE
LNQTLRNLSLIYPDGNLNFIRVKKIVTDSACFNMNHWIESILTGNKQRADRILKKLEYIG
EHHHHHHCEEEEECCCEEEEEEEEEHHCHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHCC
VDLELLLCRIKSEILMITNMKYNMVQGKSLFNLLKKYKIYRKYHCMLLARAVNRLSLYQL
CCHHHHHHHHCCCEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQAIELLAQIELIYKKDYIVLSRSSFELLSLILCYDKKVSLK
HHHHHHHHHHHHHEECCEEEEECCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8505303; 1400251; 8905232; 9278503; 3316191; 8505304; 1575709 [H]