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The map label for this gene is ygfZ [C]

Identifier: 71892042

GI number: 71892042

Start: 305107

End: 306099

Strand: Reverse

Name: ygfZ [C]

Synonym: BPEN_268

Alternate gene names: 71892042

Gene position: 306099-305107 (Counterclockwise)

Preceding gene: 71892049

Following gene: 71892039

Centisome position: 38.67

GC content: 28.6

Gene sequence:

>993_bases
ATGTTTCCTCGTGTGGCTTTTTTGGGACAGTATCCTGTACCCTCAAAAGATTTGCCGCTAACATTCATCTCCTTAGAAGA
ATGGACGTTAGTGAGATTACATGGACCAGATGTTATACAATGTTTACACAATCAATTTACTTGTGATATTCAAAATTTGA
ATAAACATAAATATAGTTTTGCAGCACATTGTAATCCAAAAGGAAAAATGATTAGCAATCTATATGTTTTTCATCTTAAA
AATCAAGAAATGGCATTTATTGAACGTCTAAATATATGCAAAAAACAAATAGAAGAAATGAAAAAATATATGGTATTTTC
TAATGTTACTGTTATTCCTGATTATAATGCAATACTTATTGGAATAGCTGGAACAAATGCAAGAAATCATTTAAGCATGT
TTTTTTCAGTTTTACCAAATAAGACACACACAATAATTCATACTCAAGATGTGACCTTACTTTATTTGAGTTCTCCATCA
GAACGATTTTTGTTGATTATTAATAAAAAATCAGTATTAGATTATTTGTTAAATGAATCACAGTCTCAGATACAATTTAA
TGATAGTCGTCAATGGGTATCATTAGATATGGAAGCGGGTTATCCAATTATTGAGCCAATAACTAGTGAATTATTTATTC
CTCAAGCTGTTAATATGGATATATTAGATGGAATTAGTTTTAATAAGGGTTGTTATATTGGACAAGAATCTATTGCGCGT
ATTAAGTATCGTGGATATAACAAACAAACTTTATATAGGTTAAACGGTGTCATGGATTATAAAAAAAATTATAACTTGCC
TGCTGCTGGTGATCAGGTAGAATTAAAAATAAATAACCAACACTGGAAAAATGTTGGAATTGTTTTACAATCTTGTCAAA
TTAAAAAAGATAATATATGGGTACAAGTGGTATTAAATAGATCTATTTTAGAACCTTCAGAATTGAGAATTACAAATACA
CAAACTCATGATAATCTCATGTTTTATTATTGA

Upstream 100 bases:

>100_bases
TTTGTTATCTTTCGGTTGTAGATATTTAATGAGTAGGATTAATTAGTATATATTATATGATATTTATTTGAAGTTATTTT
TATTCATAAGGAAAAATATA

Downstream 100 bases:

>100_bases
TATTTGATGTATTTAAATATTTCTTATTATTTTAATATCACAATATTTATTTTTAGTCGTAATGATCTCGTAGCAACCAT
TTTGGCATATTATTTAATCC

Product: putative aminomethyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MFPRVAFLGQYPVPSKDLPLTFISLEEWTLVRLHGPDVIQCLHNQFTCDIQNLNKHKYSFAAHCNPKGKMISNLYVFHLK
NQEMAFIERLNICKKQIEEMKKYMVFSNVTVIPDYNAILIGIAGTNARNHLSMFFSVLPNKTHTIIHTQDVTLLYLSSPS
ERFLLIINKKSVLDYLLNESQSQIQFNDSRQWVSLDMEAGYPIIEPITSELFIPQAVNMDILDGISFNKGCYIGQESIAR
IKYRGYNKQTLYRLNGVMDYKKNYNLPAAGDQVELKINNQHWKNVGIVLQSCQIKKDNIWVQVVLNRSILEPSELRITNT
QTHDNLMFYY

Sequences:

>Translated_330_residues
MFPRVAFLGQYPVPSKDLPLTFISLEEWTLVRLHGPDVIQCLHNQFTCDIQNLNKHKYSFAAHCNPKGKMISNLYVFHLK
NQEMAFIERLNICKKQIEEMKKYMVFSNVTVIPDYNAILIGIAGTNARNHLSMFFSVLPNKTHTIIHTQDVTLLYLSSPS
ERFLLIINKKSVLDYLLNESQSQIQFNDSRQWVSLDMEAGYPIIEPITSELFIPQAVNMDILDGISFNKGCYIGQESIAR
IKYRGYNKQTLYRLNGVMDYKKNYNLPAAGDQVELKINNQHWKNVGIVLQSCQIKKDNIWVQVVLNRSILEPSELRITNT
QTHDNLMFYY
>Mature_330_residues
MFPRVAFLGQYPVPSKDLPLTFISLEEWTLVRLHGPDVIQCLHNQFTCDIQNLNKHKYSFAAHCNPKGKMISNLYVFHLK
NQEMAFIERLNICKKQIEEMKKYMVFSNVTVIPDYNAILIGIAGTNARNHLSMFFSVLPNKTHTIIHTQDVTLLYLSSPS
ERFLLIINKKSVLDYLLNESQSQIQFNDSRQWVSLDMEAGYPIIEPITSELFIPQAVNMDILDGISFNKGCYIGQESIAR
IKYRGYNKQTLYRLNGVMDYKKNYNLPAAGDQVELKINNQHWKNVGIVLQSCQIKKDNIWVQVVLNRSILEPSELRITNT
QTHDNLMFYY

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family

Homologues:

Organism=Escherichia coli, GI1789265, Length=313, Percent_Identity=38.9776357827476, Blast_Score=243, Evalue=1e-65,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): YGFZ_BLOPB (Q493E3)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277772.1
- HSSP:   P0ADE8
- STRING:   Q493E3
- GeneID:   3562608
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_268
- eggNOG:   COG0354
- HOGENOM:   HBG700628
- OMA:   GAHCDPK
- ProtClustDB:   CLSK280772
- BioCyc:   CBLO291272:BPEN_268-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01175
- InterPro:   IPR017703
- InterPro:   IPR006222
- TIGRFAMs:   TIGR03317

Pfam domain/function: PF01571 GCV_T

EC number: NA

Molecular weight: Translated: 38264; Mature: 38264

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: BINDING 28-28 BINDING 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPRVAFLGQYPVPSKDLPLTFISLEEWTLVRLHGPDVIQCLHNQFTCDIQNLNKHKYSF
CCCCEEEEECCCCCCCCCCEEEEEECCEEEEEECCCHHHHHHHCCCEEEECCCCCCCEEE
AAHCNPKGKMISNLYVFHLKNQEMAFIERLNICKKQIEEMKKYMVFSNVTVIPDYNAILI
EEECCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEE
GIAGTNARNHLSMFFSVLPNKTHTIIHTQDVTLLYLSSPSERFLLIINKKSVLDYLLNES
EEECCCCHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCCEEEEEEECHHHHHHHHCCC
QSQIQFNDSRQWVSLDMEAGYPIIEPITSELFIPQAVNMDILDGISFNKGCYIGQESIAR
CCEEEECCCCEEEEEEECCCCCHHHCCHHHEECCCCCCCHHHCCCCCCCCEEECHHHHEE
IKYRGYNKQTLYRLNGVMDYKKNYNLPAAGDQVELKINNQHWKNVGIVLQSCQIKKDNIW
EEECCCCCCEEEEECCEEEHHHCCCCCCCCCEEEEEECCCCCCEEEEEEEEEEEECCCEE
VQVVLNRSILEPSELRITNTQTHDNLMFYY
EEEEECCCCCCCCCEEEEECCCCCCEEEEC
>Mature Secondary Structure
MFPRVAFLGQYPVPSKDLPLTFISLEEWTLVRLHGPDVIQCLHNQFTCDIQNLNKHKYSF
CCCCEEEEECCCCCCCCCCEEEEEECCEEEEEECCCHHHHHHHCCCEEEECCCCCCCEEE
AAHCNPKGKMISNLYVFHLKNQEMAFIERLNICKKQIEEMKKYMVFSNVTVIPDYNAILI
EEECCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEE
GIAGTNARNHLSMFFSVLPNKTHTIIHTQDVTLLYLSSPSERFLLIINKKSVLDYLLNES
EEECCCCHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCCEEEEEEECHHHHHHHHCCC
QSQIQFNDSRQWVSLDMEAGYPIIEPITSELFIPQAVNMDILDGISFNKGCYIGQESIAR
CCEEEECCCCEEEEEEECCCCCHHHCCHHHEECCCCCCCHHHCCCCCCCCEEECHHHHEE
IKYRGYNKQTLYRLNGVMDYKKNYNLPAAGDQVELKINNQHWKNVGIVLQSCQIKKDNIW
EEECCCCCCEEEEECCEEEHHHCCCCCCCCCEEEEEECCCCCCEEEEEEEEEEEECCCEE
VQVVLNRSILEPSELRITNTQTHDNLMFYY
EEEEECCCCCCCCCEEEEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA