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The map label for this gene is amiB2 [H]

Identifier: 68535994

GI number: 68535994

Start: 1095473

End: 1096555

Strand: Direct

Name: amiB2 [H]

Synonym: jk0917

Alternate gene names: 68535994

Gene position: 1095473-1096555 (Clockwise)

Preceding gene: 68535993

Following gene: 68535996

Centisome position: 44.49

GC content: 64.54

Gene sequence:

>1083_bases
GTGCCGGTGCTCATTAAGGAAGAAGTTGATGTCCGCGGGGTGCCCACGACCTACGGCACCAATGCCAACCCCACGCCCGC
GGCCTCCGACGACGAGATCGTCCGCCGACTCCGTGCCAGCGGTGCGATCATCCTGGGCAAAACCACTATGCCTGCCTTTG
GCGCTTTCCCTTTCACCGAGTCGGAGGCTCGCGGTATCACCCGCAACCCGCACAACCCGCGCTTCACCCCTGGCGGCTCT
TCCGGAGGTTCCGCGGCTGCCGTTGCCGCTGGATGGGTGCCCTTCGCCGTTGGTGGCGACGGCGGCGGTTCTATCCGCAT
CCCCTCGGCCCATTGCGGTCTCTTTGGCCTCAAACCCGCCCGCGGTACTCTCCCCGGTGAACTGTGGTGCCGCCTCGGCA
CTGCTGGCCCCATCGCCCGTACTGCACGCGACTGCGCCCTTCTTTTTGACGCCCTATCTGGCTCCGAAGCCACCGAATCT
GAGGTGGCGTCATTAAGGATTGGCGTAAACCTCCAACCGGCAACGCCACTGACCCCACCACACCGCGACCATGTCCGGGC
AGTATGCCGTGTTGCCGATCAGCTGCGAGGGCTGGGACACACTGTGAGCGACTATGCGCTGCGGCACAAGGATCCGACGG
CGGCTTTCGTCACGCAGTTCTTCGCGGGGATTCGGGAGGAGATCGCCGGGCCGGAGTATCCCGAACGTATCGAGGGGCGC
CACAAAGTTACGCACCTGGCAGGTGCATGGGCCCAGGGGCCGGTGCTGCGTTGGGCGGAGAAGTTCTCCGACGAGCTGGG
GGAGGAGCTGGATCGGGTGTTCGAGGACGTTGACGTGCTCCTTACACCAACGGTGGCCTCGCGCCCGGCGCGCGCTGGTG
TGCTTTCCGGGAAGGGGATGTTGGCGGCACAGATCGCGAGTTTGCCCTCGGTTGCCTTCACAGCGCTGTGGAACGTTTCC
GGGCATCCGGCGATGAACATCCCCGCAGGTAGAGGAAGAGATGGCCTGCCCGTTGGTGTACAGCTCGTAGCACGCGACGA
GCGGCTACTTCTTAACCTTGCCGCTTCATTATCCGCGCCATAG

Upstream 100 bases:

>100_bases
GCACCTTCCTCGGAGGCTGCATCCACTCCGGCAAGCGTGTTGGCGAGAACCTGAAGGCGACCATTGCTGAGCTCTAGTCA
GTGGCGCACCGCTGCATGGC

Downstream 100 bases:

>100_bases
CTTCCGTCATGACGCGCCGTGGCATGCGCTGCGAGATGCTTGTTAGCACCTTGTTGCGCGCGCCCGCGACTACCGACGGC
GGCGGCGACTTGCGCAGTAG

Product: putative amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MPVLIKEEVDVRGVPTTYGTNANPTPAASDDEIVRRLRASGAIILGKTTMPAFGAFPFTESEARGITRNPHNPRFTPGGS
SGGSAAAVAAGWVPFAVGGDGGGSIRIPSAHCGLFGLKPARGTLPGELWCRLGTAGPIARTARDCALLFDALSGSEATES
EVASLRIGVNLQPATPLTPPHRDHVRAVCRVADQLRGLGHTVSDYALRHKDPTAAFVTQFFAGIREEIAGPEYPERIEGR
HKVTHLAGAWAQGPVLRWAEKFSDELGEELDRVFEDVDVLLTPTVASRPARAGVLSGKGMLAAQIASLPSVAFTALWNVS
GHPAMNIPAGRGRDGLPVGVQLVARDERLLLNLAASLSAP

Sequences:

>Translated_360_residues
MPVLIKEEVDVRGVPTTYGTNANPTPAASDDEIVRRLRASGAIILGKTTMPAFGAFPFTESEARGITRNPHNPRFTPGGS
SGGSAAAVAAGWVPFAVGGDGGGSIRIPSAHCGLFGLKPARGTLPGELWCRLGTAGPIARTARDCALLFDALSGSEATES
EVASLRIGVNLQPATPLTPPHRDHVRAVCRVADQLRGLGHTVSDYALRHKDPTAAFVTQFFAGIREEIAGPEYPERIEGR
HKVTHLAGAWAQGPVLRWAEKFSDELGEELDRVFEDVDVLLTPTVASRPARAGVLSGKGMLAAQIASLPSVAFTALWNVS
GHPAMNIPAGRGRDGLPVGVQLVARDERLLLNLAASLSAP
>Mature_359_residues
PVLIKEEVDVRGVPTTYGTNANPTPAASDDEIVRRLRASGAIILGKTTMPAFGAFPFTESEARGITRNPHNPRFTPGGSS
GGSAAAVAAGWVPFAVGGDGGGSIRIPSAHCGLFGLKPARGTLPGELWCRLGTAGPIARTARDCALLFDALSGSEATESE
VASLRIGVNLQPATPLTPPHRDHVRAVCRVADQLRGLGHTVSDYALRHKDPTAAFVTQFFAGIREEIAGPEYPERIEGRH
KVTHLAGAWAQGPVLRWAEKFSDELGEELDRVFEDVDVLLTPTVASRPARAGVLSGKGMLAAQIASLPSVAFTALWNVSG
HPAMNIPAGRGRDGLPVGVQLVARDERLLLNLAASLSAP

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=163, Percent_Identity=32.5153374233129, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI222831590, Length=434, Percent_Identity=23.5023041474654, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17537465, Length=402, Percent_Identity=25.1243781094527, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=400, Percent_Identity=26.75, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=128, Percent_Identity=38.28125, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=157, Percent_Identity=33.1210191082803, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17538252, Length=231, Percent_Identity=28.1385281385281, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320448, Length=158, Percent_Identity=37.3417721518987, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=124, Percent_Identity=39.5161290322581, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI161078093, Length=409, Percent_Identity=28.6063569682152, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI21356731, Length=402, Percent_Identity=25.8706467661692, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24644968, Length=413, Percent_Identity=24.9394673123487, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24648441, Length=401, Percent_Identity=26.4339152119701, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648439, Length=401, Percent_Identity=26.4339152119701, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648435, Length=401, Percent_Identity=26.4339152119701, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24648437, Length=401, Percent_Identity=26.4339152119701, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI45550774, Length=401, Percent_Identity=26.4339152119701, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24652985, Length=408, Percent_Identity=26.2254901960784, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19922090, Length=408, Percent_Identity=26.2254901960784, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24652981, Length=408, Percent_Identity=26.2254901960784, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24652983, Length=408, Percent_Identity=26.2254901960784, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648113, Length=415, Percent_Identity=26.7469879518072, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 37798; Mature: 37667

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLIKEEVDVRGVPTTYGTNANPTPAASDDEIVRRLRASGAIILGKTTMPAFGAFPFTE
CCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCC
SEARGITRNPHNPRFTPGGSSGGSAAAVAAGWVPFAVGGDGGGSIRIPSAHCGLFGLKPA
HHCCCCCCCCCCCCCCCCCCCCCCHHHHEECCEEEEEECCCCCEEECCCCCCCCEECCCC
RGTLPGELWCRLGTAGPIARTARDCALLFDALSGSEATESEVASLRIGVNLQPATPLTPP
CCCCCHHHHEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHEEEECEEECCCCCCCCC
HRDHVRAVCRVADQLRGLGHTVSDYALRHKDPTAAFVTQFFAGIREEIAGPEYPERIEGR
CHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCH
HKVTHLAGAWAQGPVLRWAEKFSDELGEELDRVFEDVDVLLTPTVASRPARAGVLSGKGM
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHCCCCCCCEECCCCH
LAAQIASLPSVAFTALWNVSGHPAMNIPAGRGRDGLPVGVQLVARDERLLLNLAASLSAP
HHHHHHHCCHHHHHHEECCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCC
>Mature Secondary Structure 
PVLIKEEVDVRGVPTTYGTNANPTPAASDDEIVRRLRASGAIILGKTTMPAFGAFPFTE
CEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCC
SEARGITRNPHNPRFTPGGSSGGSAAAVAAGWVPFAVGGDGGGSIRIPSAHCGLFGLKPA
HHCCCCCCCCCCCCCCCCCCCCCCHHHHEECCEEEEEECCCCCEEECCCCCCCCEECCCC
RGTLPGELWCRLGTAGPIARTARDCALLFDALSGSEATESEVASLRIGVNLQPATPLTPP
CCCCCHHHHEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHEEEECEEECCCCCCCCC
HRDHVRAVCRVADQLRGLGHTVSDYALRHKDPTAAFVTQFFAGIREEIAGPEYPERIEGR
CHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCH
HKVTHLAGAWAQGPVLRWAEKFSDELGEELDRVFEDVDVLLTPTVASRPARAGVLSGKGM
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHCCCCCCCEECCCCH
LAAQIASLPSVAFTALWNVSGHPAMNIPAGRGRDGLPVGVQLVARDERLLLNLAASLSAP
HHHHHHHCCHHHHHHEECCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]