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The map label for this gene is polA [H]

Identifier: 68535893

GI number: 68535893

Start: 970489

End: 973068

Strand: Direct

Name: polA [H]

Synonym: jk0818

Alternate gene names: 68535893

Gene position: 970489-973068 (Clockwise)

Preceding gene: 68535892

Following gene: 161986623

Centisome position: 39.41

GC content: 63.57

Gene sequence:

>2580_bases
ATGGCTAAACTCCTTCTCCTCGATGGGCATTCGTTGGCGTTCCGCGCCTTCTATGCTCTGCCCGCAGCCAATTTTTCCAC
CGTCGGTGGCCAGCACACCAACGCGGTGTATGGCTTCTTAGGCATGTTCCTGACTCTTATGGATAAGGAACAGCCCACCC
ATGTGGCCGCCGCGTTTGACCTGTCCGGCCCCACATTCCGCGAGGAATCCTTCCCGGAATACAAGGCGCAGCGCCCGAGC
ACGCCCGAGGAGTTTAAGGGTCAGATCGAGCTGATTCGTCGCACGCTCGAAGCGTTGGGCGTCACGACCCTAGACAAAGA
GTCCTACGAGGCCGACGACATCATCGCGACCCTAGCCACCGAGGGTGCCAAGGATGGCATGGACGTGCTGATCTGCACCG
GCGACCGCGATTCGTTGCAGCTGGTCACCGAGGATGTGACGGTGCTGTATACGCTCAAGGGTGTCTCTGAGCTGCATCGT
TTCACCCCACAGGCCGTCGAAGAGAAGTACGGTGTGACCCCCACCCAGTATCCGGACCTCGCGGCACTGCGCGGTGATAC
CTCCGACAACATGCCCGGCGTGCCCGGCGTGGGCCCGAAGACTGCGCAGAAGTGGATCGCCAAGTACGGCAGCCTGCAGG
GCGTGATCGACAACATGGATGACATCGGCGGGAAGGTCGGCGCCAACCTGCGTGAAAACATCGACAATGTGGTGCTCGCG
CGCGACATTACGGAAATGGTGCGGGACCTTAAGCTTGTTGATGATTTGGACGCCCTCAGTCCCCACGACATCGATCCCAA
CGAGGCGCTCGGCATGTTCGAGGAGCTGCAGTTTGGCACTGGCCTGCGCAATAGGGCATTCAAGATCATGGGCGTGGAGT
TCGCTGACAGCGCTGCGGTGCCCGTCACCGTGGAAAAGCCGAAGCCGGGGAAGCTGGGCGCGTGGCTGAAAAAGCGCACC
CGCGGCACGGTGGTAGACGTGGATGGCGAGCAGCTGACCCTGCTGGACCGCGACAACGTCGTCATCGTTCAAGACCTCGC
CGACGTGGCCCCGAAGGACGAGAAGGCGCTGGCGGAGTGGCTGGCCAACCCACAGGCCACGAAGTGGCTCCACGATTCGA
AGAAGGCCTACCACACGCTTCAGAATCAAGGCTTGACCCTTGATGGTGTGGAGCATGATACGTCCCTGGCGGCGTACCTG
CTGCGCCCCGACGTGCGCACCCTCTCCCTGGCGGACGTCGTGCAGCGCCACGTGGGTCACGAACTGTCGGAGGGCGCCAC
CGGCCCCGAGCGCGCCCAGGCTGTCGCCGAACTGGTGAAGGCCCTCACTCCAGAGATCGAGGCTGCCGGCCAGGCCGAAC
TATACTTTGACCTGGAAGTCCCCCTGGCCCTGATCCTCGCGGCCATGGAACACGCGGGCATTGCCGTGGACGACGCAGCG
CTGCAGGAGCTCTCCGACGACTACGGCGCACGCCTTAACGAGGAGATCGCCGCCGCCCGCGAACTGGCGGGGGAGCCGGA
GCTGAACCTTTCCTCCCCGAAGCAGCTGCAGAAGGTCCTCTTCGAGACCTTCGACCTGCCGAAGACGAAGAAGACCAAGA
CCGGGTATTCCACCGCCGCCGCGGAACTGGAAAAACTGGCCGCCCAGTCCAACCACCCGTTCCTGGGGCACCTCATGGCC
CACCGCGAGTACCAGAAGATGAAGTCCACCATCGACGGACTGATCGAGGCCGTGGGCGACGACGGGCGCATCCACACCAC
CTTCAACCAGAAGGGCGCGGCGACGGGCCGCCTGTCTTCCTCCGATCCGAACCTGCAGAACATCCCCGTGCGCACCGACG
CCGGCCGCAAGATCCGCGCCGCGTTCACGGTCGGGGAGGGCTACGAAACTCTGCTGACCGCCGACTACTCCCAGATCGAG
ATGCGCGTGATGGCGCACCTCTCGGAAGATGCGGGCCTGATCGAGGCCTACGCCAGCGGCGAGGATCTGCACAATTACGT
CGGCTCGCGCGTATTCGACGTTCCGGTCGATGAGGTCACCCCGGAGTTGCGCCGCCGGGTGAAGGCGCTGAGCTATGGCC
TGGTCTACGGCCTGTCCGCCTTCGGCCTATCTCAGCAGCTGAAGATCAGCGCGTGGGAGGCGAAGAGAATTATGGATAAC
TACTTCGAGCGTTTCGGAGGTGTGAAGCGCTATCTCGACCACGTGGTGGAGGTTGCCAAGGAAACCGGCTACACGGAGAC
TCTCTACGGCCGCCGTCGCTACCTGCCGGAGCTGAACTCCTCCAATCGTGTGGCCAGAGAGAATGCCGAGCGCGCCGCCC
TCAACGCGCCGATCCAGGGCACGGCCGCGGACATCATCAAGCTGGCCATGCTGCGCGTGGATCGGGAGATCACCCGTCAG
CAGCTGGCTTCCCGCGTGTTGCTGCAGGTCCACGATGAACTCGTGGTGGAAGTCGCGCCCGGCGAGCTGGAGCAGATCAC
CGAGCTAGTGGAGCGCGAGATGGATGCCGCCGCCCAGTTGCGCGTACCGCTGGATGTCTCCGCCGGCACGGGCGATAACT
GGGATCTGGCAGCGCACTAG

Upstream 100 bases:

>100_bases
CCTCTGCCAATTGGGCTACTGGGGCGATAAGTGCTTACCGGAGCATAATACGCCACGGCCTTTCAGCCAACCAAAACCGC
ACTCCGTTAGAGTTGAGCGC

Downstream 100 bases:

>100_bases
CCACGCGCGCCAGGCGTGTTAGCTGGCGTCTGCCTGCGTGGTCTTCGCGTTCGAGGCCCTCTCGGCCGCCAGCATCGCCA
TGACGATCACCGCGCCCAGC

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 859; Mature: 858

Protein sequence:

>859_residues
MAKLLLLDGHSLAFRAFYALPAANFSTVGGQHTNAVYGFLGMFLTLMDKEQPTHVAAAFDLSGPTFREESFPEYKAQRPS
TPEEFKGQIELIRRTLEALGVTTLDKESYEADDIIATLATEGAKDGMDVLICTGDRDSLQLVTEDVTVLYTLKGVSELHR
FTPQAVEEKYGVTPTQYPDLAALRGDTSDNMPGVPGVGPKTAQKWIAKYGSLQGVIDNMDDIGGKVGANLRENIDNVVLA
RDITEMVRDLKLVDDLDALSPHDIDPNEALGMFEELQFGTGLRNRAFKIMGVEFADSAAVPVTVEKPKPGKLGAWLKKRT
RGTVVDVDGEQLTLLDRDNVVIVQDLADVAPKDEKALAEWLANPQATKWLHDSKKAYHTLQNQGLTLDGVEHDTSLAAYL
LRPDVRTLSLADVVQRHVGHELSEGATGPERAQAVAELVKALTPEIEAAGQAELYFDLEVPLALILAAMEHAGIAVDDAA
LQELSDDYGARLNEEIAAARELAGEPELNLSSPKQLQKVLFETFDLPKTKKTKTGYSTAAAELEKLAAQSNHPFLGHLMA
HREYQKMKSTIDGLIEAVGDDGRIHTTFNQKGAATGRLSSSDPNLQNIPVRTDAGRKIRAAFTVGEGYETLLTADYSQIE
MRVMAHLSEDAGLIEAYASGEDLHNYVGSRVFDVPVDEVTPELRRRVKALSYGLVYGLSAFGLSQQLKISAWEAKRIMDN
YFERFGGVKRYLDHVVEVAKETGYTETLYGRRRYLPELNSSNRVARENAERAALNAPIQGTAADIIKLAMLRVDREITRQ
QLASRVLLQVHDELVVEVAPGELEQITELVEREMDAAAQLRVPLDVSAGTGDNWDLAAH

Sequences:

>Translated_859_residues
MAKLLLLDGHSLAFRAFYALPAANFSTVGGQHTNAVYGFLGMFLTLMDKEQPTHVAAAFDLSGPTFREESFPEYKAQRPS
TPEEFKGQIELIRRTLEALGVTTLDKESYEADDIIATLATEGAKDGMDVLICTGDRDSLQLVTEDVTVLYTLKGVSELHR
FTPQAVEEKYGVTPTQYPDLAALRGDTSDNMPGVPGVGPKTAQKWIAKYGSLQGVIDNMDDIGGKVGANLRENIDNVVLA
RDITEMVRDLKLVDDLDALSPHDIDPNEALGMFEELQFGTGLRNRAFKIMGVEFADSAAVPVTVEKPKPGKLGAWLKKRT
RGTVVDVDGEQLTLLDRDNVVIVQDLADVAPKDEKALAEWLANPQATKWLHDSKKAYHTLQNQGLTLDGVEHDTSLAAYL
LRPDVRTLSLADVVQRHVGHELSEGATGPERAQAVAELVKALTPEIEAAGQAELYFDLEVPLALILAAMEHAGIAVDDAA
LQELSDDYGARLNEEIAAARELAGEPELNLSSPKQLQKVLFETFDLPKTKKTKTGYSTAAAELEKLAAQSNHPFLGHLMA
HREYQKMKSTIDGLIEAVGDDGRIHTTFNQKGAATGRLSSSDPNLQNIPVRTDAGRKIRAAFTVGEGYETLLTADYSQIE
MRVMAHLSEDAGLIEAYASGEDLHNYVGSRVFDVPVDEVTPELRRRVKALSYGLVYGLSAFGLSQQLKISAWEAKRIMDN
YFERFGGVKRYLDHVVEVAKETGYTETLYGRRRYLPELNSSNRVARENAERAALNAPIQGTAADIIKLAMLRVDREITRQ
QLASRVLLQVHDELVVEVAPGELEQITELVEREMDAAAQLRVPLDVSAGTGDNWDLAAH
>Mature_858_residues
AKLLLLDGHSLAFRAFYALPAANFSTVGGQHTNAVYGFLGMFLTLMDKEQPTHVAAAFDLSGPTFREESFPEYKAQRPST
PEEFKGQIELIRRTLEALGVTTLDKESYEADDIIATLATEGAKDGMDVLICTGDRDSLQLVTEDVTVLYTLKGVSELHRF
TPQAVEEKYGVTPTQYPDLAALRGDTSDNMPGVPGVGPKTAQKWIAKYGSLQGVIDNMDDIGGKVGANLRENIDNVVLAR
DITEMVRDLKLVDDLDALSPHDIDPNEALGMFEELQFGTGLRNRAFKIMGVEFADSAAVPVTVEKPKPGKLGAWLKKRTR
GTVVDVDGEQLTLLDRDNVVIVQDLADVAPKDEKALAEWLANPQATKWLHDSKKAYHTLQNQGLTLDGVEHDTSLAAYLL
RPDVRTLSLADVVQRHVGHELSEGATGPERAQAVAELVKALTPEIEAAGQAELYFDLEVPLALILAAMEHAGIAVDDAAL
QELSDDYGARLNEEIAAARELAGEPELNLSSPKQLQKVLFETFDLPKTKKTKTGYSTAAAELEKLAAQSNHPFLGHLMAH
REYQKMKSTIDGLIEAVGDDGRIHTTFNQKGAATGRLSSSDPNLQNIPVRTDAGRKIRAAFTVGEGYETLLTADYSQIEM
RVMAHLSEDAGLIEAYASGEDLHNYVGSRVFDVPVDEVTPELRRRVKALSYGLVYGLSAFGLSQQLKISAWEAKRIMDNY
FERFGGVKRYLDHVVEVAKETGYTETLYGRRRYLPELNSSNRVARENAERAALNAPIQGTAADIIKLAMLRVDREITRQQ
LASRVLLQVHDELVVEVAPGELEQITELVEREMDAAAQLRVPLDVSAGTGDNWDLAAH

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=516, Percent_Identity=31.3953488372093, Blast_Score=225, Evalue=1e-58,
Organism=Homo sapiens, GI139394648, Length=626, Percent_Identity=24.7603833865815, Blast_Score=182, Evalue=9e-46,
Organism=Escherichia coli, GI1790294, Length=932, Percent_Identity=37.0171673819742, Blast_Score=514, Evalue=1e-146,
Organism=Escherichia coli, GI226510970, Length=216, Percent_Identity=31.0185185185185, Blast_Score=119, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI86563272, Length=427, Percent_Identity=29.5081967213115, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI17933644, Length=553, Percent_Identity=30.0180831826401, Blast_Score=213, Evalue=4e-55,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 94224; Mature: 94092

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLLLLDGHSLAFRAFYALPAANFSTVGGQHTNAVYGFLGMFLTLMDKEQPTHVAAAFD
CCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEE
LSGPTFREESFPEYKAQRPSTPEEFKGQIELIRRTLEALGVTTLDKESYEADDIIATLAT
CCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
EGAKDGMDVLICTGDRDSLQLVTEDVTVLYTLKGVSELHRFTPQAVEEKYGVTPTQYPDL
CCCCCCCEEEEEECCCCCHHHHCCCEEEEEEHHHHHHHHHCCHHHHHHHCCCCCCCCCCC
AALRGDTSDNMPGVPGVGPKTAQKWIAKYGSLQGVIDNMDDIGGKVGANLRENIDNVVLA
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCHHHCCHHCCCCHHHHHHHHHHH
RDITEMVRDLKLVDDLDALSPHDIDPNEALGMFEELQFGTGLRNRAFKIMGVEFADSAAV
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCC
PVTVEKPKPGKLGAWLKKRTRGTVVDVDGEQLTLLDRDNVVIVQDLADVAPKDEKALAEW
EEEECCCCCCCHHHHHHHCCCCCEEEECCCEEEEEECCCEEEEECHHHCCCCCHHHHHHH
LANPQATKWLHDSKKAYHTLQNQGLTLDGVEHDTSLAAYLLRPDVRTLSLADVVQRHVGH
HCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCC
ELSEGATGPERAQAVAELVKALTPEIEAAGQAELYFDLEVPLALILAAMEHAGIAVDDAA
HHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCEECHHH
LQELSDDYGARLNEEIAAARELAGEPELNLSSPKQLQKVLFETFDLPKTKKTKTGYSTAA
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHH
AELEKLAAQSNHPFLGHLMAHREYQKMKSTIDGLIEAVGDDGRIHTTFNQKGAATGRLSS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCC
SDPNLQNIPVRTDAGRKIRAAFTVGEGYETLLTADYSQIEMRVMAHLSEDAGLIEAYASG
CCCCCCCCCEECCCCCEEEEEEEECCCCHHHHHCCHHHHHHHHHHHHCCCCCHHHHHCCC
EDLHNYVGSRVFDVPVDEVTPELRRRVKALSYGLVYGLSAFGLSQQLKISAWEAKRIMDN
HHHHHHHCCEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHH
YFERFGGVKRYLDHVVEVAKETGYTETLYGRRRYLPELNSSNRVARENAERAALNAPIQG
HHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCC
TAADIIKLAMLRVDREITRQQLASRVLLQVHDELVVEVAPGELEQITELVEREMDAAAQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCHHEE
RVPLDVSAGTGDNWDLAAH
ECEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
AKLLLLDGHSLAFRAFYALPAANFSTVGGQHTNAVYGFLGMFLTLMDKEQPTHVAAAFD
CEEEEECCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEE
LSGPTFREESFPEYKAQRPSTPEEFKGQIELIRRTLEALGVTTLDKESYEADDIIATLAT
CCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
EGAKDGMDVLICTGDRDSLQLVTEDVTVLYTLKGVSELHRFTPQAVEEKYGVTPTQYPDL
CCCCCCCEEEEEECCCCCHHHHCCCEEEEEEHHHHHHHHHCCHHHHHHHCCCCCCCCCCC
AALRGDTSDNMPGVPGVGPKTAQKWIAKYGSLQGVIDNMDDIGGKVGANLRENIDNVVLA
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCHHHCCHHCCCCHHHHHHHHHHH
RDITEMVRDLKLVDDLDALSPHDIDPNEALGMFEELQFGTGLRNRAFKIMGVEFADSAAV
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCC
PVTVEKPKPGKLGAWLKKRTRGTVVDVDGEQLTLLDRDNVVIVQDLADVAPKDEKALAEW
EEEECCCCCCCHHHHHHHCCCCCEEEECCCEEEEEECCCEEEEECHHHCCCCCHHHHHHH
LANPQATKWLHDSKKAYHTLQNQGLTLDGVEHDTSLAAYLLRPDVRTLSLADVVQRHVGH
HCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCC
ELSEGATGPERAQAVAELVKALTPEIEAAGQAELYFDLEVPLALILAAMEHAGIAVDDAA
HHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCEECHHH
LQELSDDYGARLNEEIAAARELAGEPELNLSSPKQLQKVLFETFDLPKTKKTKTGYSTAA
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHH
AELEKLAAQSNHPFLGHLMAHREYQKMKSTIDGLIEAVGDDGRIHTTFNQKGAATGRLSS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCC
SDPNLQNIPVRTDAGRKIRAAFTVGEGYETLLTADYSQIEMRVMAHLSEDAGLIEAYASG
CCCCCCCCCEECCCCCEEEEEEEECCCCHHHHHCCHHHHHHHHHHHHCCCCCHHHHHCCC
EDLHNYVGSRVFDVPVDEVTPELRRRVKALSYGLVYGLSAFGLSQQLKISAWEAKRIMDN
HHHHHHHCCEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHH
YFERFGGVKRYLDHVVEVAKETGYTETLYGRRRYLPELNSSNRVARENAERAALNAPIQG
HHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCC
TAADIIKLAMLRVDREITRQQLASRVLLQVHDELVVEVAPGELEQITELVEREMDAAAQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCHHEE
RVPLDVSAGTGDNWDLAAH
ECEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7476188; 11234002 [H]