Click here to switch to the map view.

The map label for this gene is hisH

Identifier: 68535865

GI number: 68535865

Start: 944349

End: 945077

Strand: Direct

Name: hisH

Synonym: jk0790

Alternate gene names: 68535865

Gene position: 944349-945077 (Clockwise)

Preceding gene: 68535864

Following gene: 68535866

Centisome position: 38.35

GC content: 65.98

Gene sequence:

>729_bases
ATGAATTCTTCTCTTTCTTCTCGACGCCCACATGTCGCCCTCCTGGACTACGGCAGCGGAAACGTACGCTCCGCCCAGCG
AGCGGTTGAAAGGGCGGGTGCCGAGGTCGAGGTGACGCGCGACCCGGAGACGGTACAGCGGGCCGACGGGCTGCTAGTGC
CGGGTGTGGGAGCCTACAGTGCGTGCATGAAGCAGCTGCACAGCGTGTATGGGGATCGTCTGATCGGCCAGCGACTGGCG
GGCGGGCGCCCGGTGCTGGGCATCTGCGTGGGCATGCAGATCCTCTTCGACCGGGGAGTGGAGTTCGCGGAAGGCACGGG
AGAAGCTGGCGCGGGGGAGGCAGCCTTTACGGAGGGCATGGGCGAGTGGCCCGGCACCGTGGAGAAGCTGGATGCTGACG
TGCTGCCGCACATGGGGTGGAACACCGTAGAAGTCGGCGACGGCTCATCCGCCAGTGCGCTCGGCAAACCCGCGGCCGAG
CCGACGAAGATGTTCGCCGGGATCGAGGAGACCGAGCGTTTCTACTTCGTCCACTCCTATGGCGTGCAGGAGTGGGAGCT
GTTGACCGATGGACGCACCGAGCCGCCGCTGGTGCACTGGGCGAGCCACGGCCGCAGCCGCTTCGTCGCAGCCGTGGAAA
ATGGCCCGCTGTGGGCTACGCAGTTCCACCCGGAAAAGTCCGGTGACGCAGGACTAAAGCTGCTGCAGAACTGGGTGGAC
ACGCTCTAG

Upstream 100 bases:

>100_bases
GGCGTCACCGCGTCGCCGAGTACGGCAATGTGCGCGAAACATTGCGTCGAGTGCGACGACGTGAACTGCGCCCGGGTAAG
TCCGGCTAGGATTACCAGTT

Downstream 100 bases:

>100_bases
CTCTAGAAATAGCACTAGTTTCATGGATTTGATCTTCGCCGACCTCGATACACGTTAGGATTCTCCCTATGGCTTCTACT
GATAACTCCCGTGCCCTGAC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MNSSLSSRRPHVALLDYGSGNVRSAQRAVERAGAEVEVTRDPETVQRADGLLVPGVGAYSACMKQLHSVYGDRLIGQRLA
GGRPVLGICVGMQILFDRGVEFAEGTGEAGAGEAAFTEGMGEWPGTVEKLDADVLPHMGWNTVEVGDGSSASALGKPAAE
PTKMFAGIEETERFYFVHSYGVQEWELLTDGRTEPPLVHWASHGRSRFVAAVENGPLWATQFHPEKSGDAGLKLLQNWVD
TL

Sequences:

>Translated_242_residues
MNSSLSSRRPHVALLDYGSGNVRSAQRAVERAGAEVEVTRDPETVQRADGLLVPGVGAYSACMKQLHSVYGDRLIGQRLA
GGRPVLGICVGMQILFDRGVEFAEGTGEAGAGEAAFTEGMGEWPGTVEKLDADVLPHMGWNTVEVGDGSSASALGKPAAE
PTKMFAGIEETERFYFVHSYGVQEWELLTDGRTEPPLVHWASHGRSRFVAAVENGPLWATQFHPEKSGDAGLKLLQNWVD
TL
>Mature_242_residues
MNSSLSSRRPHVALLDYGSGNVRSAQRAVERAGAEVEVTRDPETVQRADGLLVPGVGAYSACMKQLHSVYGDRLIGQRLA
GGRPVLGICVGMQILFDRGVEFAEGTGEAGAGEAAFTEGMGEWPGTVEKLDADVLPHMGWNTVEVGDGSSASALGKPAAE
PTKMFAGIEETERFYFVHSYGVQEWELLTDGRTEPPLVHWASHGRSRFVAAVENGPLWATQFHPEKSGDAGLKLLQNWVD
TL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=241, Percent_Identity=35.2697095435685, Blast_Score=120, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6319725, Length=239, Percent_Identity=29.7071129707113, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_CORJK (Q4JW55)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_250570.1
- ProteinModelPortal:   Q4JW55
- SMR:   Q4JW55
- STRING:   Q4JW55
- GeneID:   3433183
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0790
- NMPDR:   fig|306537.3.peg.1764
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   VCVLKEV
- ProtClustDB:   PRK13146
- BioCyc:   CJEI306537:JK0790-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 26007; Mature: 26007

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 89-89 ACT_SITE 223-223 ACT_SITE 225-225

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSLSSRRPHVALLDYGSGNVRSAQRAVERAGAEVEVTRDPETVQRADGLLVPGVGAYS
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHCCEEECCCCHHH
ACMKQLHSVYGDRLIGQRLAGGRPVLGICVGMQILFDRGVEFAEGTGEAGAGEAAFTEGM
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHCCC
GEWPGTVEKLDADVLPHMGWNTVEVGDGSSASALGKPAAEPTKMFAGIEETERFYFVHSY
CCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHCCCHHCEEEEEEEC
GVQEWELLTDGRTEPPLVHWASHGRSRFVAAVENGPLWATQFHPEKSGDAGLKLLQNWVD
CCCEEEEECCCCCCCCEEECCCCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHH
TL
CC
>Mature Secondary Structure
MNSSLSSRRPHVALLDYGSGNVRSAQRAVERAGAEVEVTRDPETVQRADGLLVPGVGAYS
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHCCEEECCCCHHH
ACMKQLHSVYGDRLIGQRLAGGRPVLGICVGMQILFDRGVEFAEGTGEAGAGEAAFTEGM
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHCCC
GEWPGTVEKLDADVLPHMGWNTVEVGDGSSASALGKPAAEPTKMFAGIEETERFYFVHSY
CCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHCCCHHCEEEEEEEC
GVQEWELLTDGRTEPPLVHWASHGRSRFVAAVENGPLWATQFHPEKSGDAGLKLLQNWVD
CCCEEEEECCCCCCCCEEECCCCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHH
TL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA