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The map label for this gene is glpF [H]

Identifier: 66766714

GI number: 66766714

Start: 431807

End: 432631

Strand: Reverse

Name: glpF [H]

Synonym: XC_0371

Alternate gene names: 66766714

Gene position: 432631-431807 (Counterclockwise)

Preceding gene: 66766715

Following gene: 66766713

Centisome position: 8.4

GC content: 64.73

Gene sequence:

>825_bases
ATGAGCCGTCAACTCGTGGGTGAGTTGATCTCCGAAGCGGTGGCGATGTTCATCATCATCGCGCTGGGCGATTCGGTCGC
GGCGATGTACATCCTCTACGACCCCAGCCCGTATCAAAACGCCTACTGGGGCGTGTGCATTGCCTGGGGCCTGGCGGTCA
CGCTGGCCATCTATGTCACCGGTTCGGTGTCCGGCACGCACGCCAACCCGGCGGTCACGCTGGCGCTGGCGTTGTTCCGC
GATTTTTCCTGGAAAAAGGTGCTGCCGTACTGGGGCGCGCAAGTGGTTGGCGCGTTTCTTGGCGCGGCGATTGTCTACCA
GCTCTACGGGCCGGTGATCGACCACTACAACCAGGTGCAGCAGCTCACCCGCGAAGCCGGCGGCGCTGCCGGGGTGTTCT
TCACCGCGCCGGGCCTTGCGGTCACGCCGATGCATGCGTTGGTGGACCAGATCATCATCACTGCGTTTCTGATCTTCGGT
ATCTTCGCCATCACCGAGCGCTTCAACGAGGCTGCGCCAACCGCCAACTCGGGCGCGTTGATGATCGGCTTGCTGGTGGC
CACGCTGGGCGCCTCGATGGGGTATCTGGAAGCCTGGGCAATGAACCCCGCGCGTGACTTCGGCCCGCGCCTGTTCGCGT
ATTTCGCCGGCTGGGGCGAAGCCGCACTGCCGGCCAAGGACAACTACTGGTGGATCCCGATCGTCGGGCCATTGATCGGT
GGCCCGCTGGGCGCCCTCGCCTACCAGTGGCTGATCCTGCCGTTCCTGCCGGCGCGGGTGTTGCACCTGCAAAAAGCCGC
CACGCCGCCACTCGATCCGCCGTGA

Upstream 100 bases:

>100_bases
GGAGGGCAACTGAGATGAGCACAACCAGACACACTTGCGCGTCGCCAGACGCGTCGCAACACCACAACGGCCATACACCG
CGCAGCGCGCAAGGCCGCCC

Downstream 100 bases:

>100_bases
TCGCACCGCTGCACTGACAGTCACGCCGTGCGCGCGGTGCGCCGGCCTTTGTTTCGAGGAATGCACGATGGAAAAGAAAT
ACGTCCTTGCGATCGACCAG

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSRQLVGELISEAVAMFIIIALGDSVAAMYILYDPSPYQNAYWGVCIAWGLAVTLAIYVTGSVSGTHANPAVTLALALFR
DFSWKKVLPYWGAQVVGAFLGAAIVYQLYGPVIDHYNQVQQLTREAGGAAGVFFTAPGLAVTPMHALVDQIIITAFLIFG
IFAITERFNEAAPTANSGALMIGLLVATLGASMGYLEAWAMNPARDFGPRLFAYFAGWGEAALPAKDNYWWIPIVGPLIG
GPLGALAYQWLILPFLPARVLHLQKAATPPLDPP

Sequences:

>Translated_274_residues
MSRQLVGELISEAVAMFIIIALGDSVAAMYILYDPSPYQNAYWGVCIAWGLAVTLAIYVTGSVSGTHANPAVTLALALFR
DFSWKKVLPYWGAQVVGAFLGAAIVYQLYGPVIDHYNQVQQLTREAGGAAGVFFTAPGLAVTPMHALVDQIIITAFLIFG
IFAITERFNEAAPTANSGALMIGLLVATLGASMGYLEAWAMNPARDFGPRLFAYFAGWGEAALPAKDNYWWIPIVGPLIG
GPLGALAYQWLILPFLPARVLHLQKAATPPLDPP
>Mature_273_residues
SRQLVGELISEAVAMFIIIALGDSVAAMYILYDPSPYQNAYWGVCIAWGLAVTLAIYVTGSVSGTHANPAVTLALALFRD
FSWKKVLPYWGAQVVGAFLGAAIVYQLYGPVIDHYNQVQQLTREAGGAAGVFFTAPGLAVTPMHALVDQIIITAFLIFGI
FAITERFNEAAPTANSGALMIGLLVATLGASMGYLEAWAMNPARDFGPRLFAYFAGWGEAALPAKDNYWWIPIVGPLIGG
PLGALAYQWLILPFLPARVLHLQKAATPPLDPP

Specific function: Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=249, Percent_Identity=38.5542168674699, Blast_Score=167, Evalue=7e-42,
Organism=Homo sapiens, GI157266307, Length=255, Percent_Identity=38.8235294117647, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI4502187, Length=266, Percent_Identity=32.3308270676692, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI22538420, Length=248, Percent_Identity=33.8709677419355, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI310133356, Length=180, Percent_Identity=31.6666666666667, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI310114181, Length=180, Percent_Identity=31.6666666666667, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI4502181, Length=247, Percent_Identity=31.1740890688259, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI4755125, Length=247, Percent_Identity=31.1740890688259, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI6912506, Length=232, Percent_Identity=31.8965517241379, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4502179, Length=273, Percent_Identity=29.6703296703297, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI37694062, Length=257, Percent_Identity=29.5719844357977, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4502183, Length=234, Percent_Identity=33.7606837606838, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI86792455, Length=211, Percent_Identity=31.2796208530806, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1790362, Length=272, Percent_Identity=39.3382352941176, Blast_Score=174, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI17544068, Length=274, Percent_Identity=34.6715328467153, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI71992966, Length=260, Percent_Identity=35.3846153846154, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71994009, Length=259, Percent_Identity=28.957528957529, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17531431, Length=252, Percent_Identity=31.3492063492063, Blast_Score=120, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17531429, Length=252, Percent_Identity=31.3492063492063, Blast_Score=120, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71992961, Length=253, Percent_Identity=32.0158102766798, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17533613, Length=247, Percent_Identity=28.3400809716599, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32564052, Length=247, Percent_Identity=28.3400809716599, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321054, Length=183, Percent_Identity=39.344262295082, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6322985, Length=214, Percent_Identity=32.7102803738318, Blast_Score=101, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45550503, Length=249, Percent_Identity=28.9156626506024, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24762342, Length=247, Percent_Identity=29.1497975708502, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 29374; Mature: 29242

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQLVGELISEAVAMFIIIALGDSVAAMYILYDPSPYQNAYWGVCIAWGLAVTLAIYVT
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEE
GSVSGTHANPAVTLALALFRDFSWKKVLPYWGAQVVGAFLGAAIVYQLYGPVIDHYNQVQ
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLTREAGGAAGVFFTAPGLAVTPMHALVDQIIITAFLIFGIFAITERFNEAAPTANSGAL
HHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
MIGLLVATLGASMGYLEAWAMNPARDFGPRLFAYFAGWGEAALPAKDNYWWIPIVGPLIG
HHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHC
GPLGALAYQWLILPFLPARVLHLQKAATPPLDPP
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SRQLVGELISEAVAMFIIIALGDSVAAMYILYDPSPYQNAYWGVCIAWGLAVTLAIYVT
CHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEE
GSVSGTHANPAVTLALALFRDFSWKKVLPYWGAQVVGAFLGAAIVYQLYGPVIDHYNQVQ
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLTREAGGAAGVFFTAPGLAVTPMHALVDQIIITAFLIFGIFAITERFNEAAPTANSGAL
HHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
MIGLLVATLGASMGYLEAWAMNPARDFGPRLFAYFAGWGEAALPAKDNYWWIPIVGPLIG
HHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHC
GPLGALAYQWLILPFLPARVLHLQKAATPPLDPP
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]