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The map label for this gene is glpK [H]

Identifier: 66766713

GI number: 66766713

Start: 430240

End: 431739

Strand: Reverse

Name: glpK [H]

Synonym: XC_0370

Alternate gene names: 66766713

Gene position: 431739-430240 (Counterclockwise)

Preceding gene: 66766714

Following gene: 66766710

Centisome position: 8.39

GC content: 63.53

Gene sequence:

>1500_bases
ATGGAAAAGAAATACGTCCTTGCGATCGACCAGGGCACCACCAGCTCGCGCGCGATGTTGTTCGATCGCCAGGGCAAGGT
GGCCGGCGTGGCGCAGCGCGAATTCGGCCAGATCTTTCCGCAGCCGGGCTGGGTGGAACACAACCCACGCGAGATCATGA
CCAGCGTCTACACCACCATTACCGAGCTGCTCAACAACGCGCAGATCGACGCGCGCGAGATCTCAGGCATCGGCATCACC
AACCAGCGCGAGACCGCGGTGGTATGGGACAAGGCCACCGGCCAACCGATCTACAACGCCATCGTCTGGCAATCGCGGCA
GACCAAGGACATCTGCACCCAGCTCAAAGAGGCCGGCCATGAGCAGATGGTGCGCGACAAGACCGGGCTGCTGATCGATG
CGTATTTTTCCGGCACCAAGGTCAAGTGGATTCTCGACCACGTGGACGGCGCACGTGAACGCGCACGCAAGGGCGAGCTC
GCCTTCGGCACCATCGATAGCTGGCTGATCTGGAACCTCACCGGCGGCAAGGTGCACGTGACCGACTACACCAATGCCTC
GCGCACCATGATGTACAACATCCACACCCTGGAGTGGGATGCCGAGCTGCTGGAGATGCTGGACGTGCCCGCGCAGATGC
TGCCGGAAGTGCGCTCGTCCAGCGAGGTCTATGGCATGACCCAGACCCAGTATTTCTACGGGGAACAGGTGCCCATTGCC
GGCATTGCCGGCGACCAGCAGGCCGCATTGTTCGGGCAAGCGTGCTTTGAACCGGGCATCGCGAAGAACACCTATGGCAC
CGGCTGTTTCATGCTGATGAATACCGGCGACAAGGCCGTGGCCTCCAAGGCCGGCTTGCTGACCACCATCGCCTGGGGCA
TCGACGGCAAGGTGGAATACGCACTGGAAGGCGCCATCTTCGTGGCTGGCTCGGTGGTGCAGTGGCTGCGCGATGGGCTG
CGCATGTTCGGCAAGGCGAGCGATTCGCAAGCCTATGCGGAGCGTGCCGGCGACAACGACGGCGTGTATTTCGTCCCGGC
CTTCGTGGGCCTGGGCGCACCGTACTGGCGCAGCGATATCCGCGGTGCGGTGTTCGGCCTGACCCGCGGCACCAGCAAGG
AACACTTCGTGCGCGCGGCGGTGGAATCGATGGCGTATCAGACCCGCGATGTGCTCACCGCGATGCAGAGCGATTCGGGG
ATCGAACTGAAGGAGCTGCGCGCCGACGGTGGCGCGATCGCCAACGACTTCATGGCGCAATTCCAGAGCGACATTCTCAA
CGTGCCGGTGCTGCGTCCGGAGGTGGCCGAAACCACCGCGCTGGGCGCGGCCTACCTGGCGGGCCTGGCCACCGGGTTCT
GGAGCAGCCGCGAAGAGATCGCCAAACAATGGGCGGTGGATCGCCGCTTCGAGCCGAACATGCCCGAAGAGCGGCGCGAA
CAGTTGTATGCCGGCTGGCAGCAGGCAGTGGAAGCCACCATGGGCTTCCGCATCAGCTAA

Upstream 100 bases:

>100_bases
AAAGCCGCCACGCCGCCACTCGATCCGCCGTGATCGCACCGCTGCACTGACAGTCACGCCGTGCGCGCGGTGCGCCGGCC
TTTGTTTCGAGGAATGCACG

Downstream 100 bases:

>100_bases
GCACTAACACCGAAAAGACTGCGCCCACCATCCGGTGGGCGCAACGTTAGGTTGGCAACTCGCGTGAGTTACGCACTGCA
GTGCTACACGCTGCAGCCCT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MEKKYVLAIDQGTTSSRAMLFDRQGKVAGVAQREFGQIFPQPGWVEHNPREIMTSVYTTITELLNNAQIDAREISGIGIT
NQRETAVVWDKATGQPIYNAIVWQSRQTKDICTQLKEAGHEQMVRDKTGLLIDAYFSGTKVKWILDHVDGARERARKGEL
AFGTIDSWLIWNLTGGKVHVTDYTNASRTMMYNIHTLEWDAELLEMLDVPAQMLPEVRSSSEVYGMTQTQYFYGEQVPIA
GIAGDQQAALFGQACFEPGIAKNTYGTGCFMLMNTGDKAVASKAGLLTTIAWGIDGKVEYALEGAIFVAGSVVQWLRDGL
RMFGKASDSQAYAERAGDNDGVYFVPAFVGLGAPYWRSDIRGAVFGLTRGTSKEHFVRAAVESMAYQTRDVLTAMQSDSG
IELKELRADGGAIANDFMAQFQSDILNVPVLRPEVAETTALGAAYLAGLATGFWSSREEIAKQWAVDRRFEPNMPEERRE
QLYAGWQQAVEATMGFRIS

Sequences:

>Translated_499_residues
MEKKYVLAIDQGTTSSRAMLFDRQGKVAGVAQREFGQIFPQPGWVEHNPREIMTSVYTTITELLNNAQIDAREISGIGIT
NQRETAVVWDKATGQPIYNAIVWQSRQTKDICTQLKEAGHEQMVRDKTGLLIDAYFSGTKVKWILDHVDGARERARKGEL
AFGTIDSWLIWNLTGGKVHVTDYTNASRTMMYNIHTLEWDAELLEMLDVPAQMLPEVRSSSEVYGMTQTQYFYGEQVPIA
GIAGDQQAALFGQACFEPGIAKNTYGTGCFMLMNTGDKAVASKAGLLTTIAWGIDGKVEYALEGAIFVAGSVVQWLRDGL
RMFGKASDSQAYAERAGDNDGVYFVPAFVGLGAPYWRSDIRGAVFGLTRGTSKEHFVRAAVESMAYQTRDVLTAMQSDSG
IELKELRADGGAIANDFMAQFQSDILNVPVLRPEVAETTALGAAYLAGLATGFWSSREEIAKQWAVDRRFEPNMPEERRE
QLYAGWQQAVEATMGFRIS
>Mature_499_residues
MEKKYVLAIDQGTTSSRAMLFDRQGKVAGVAQREFGQIFPQPGWVEHNPREIMTSVYTTITELLNNAQIDAREISGIGIT
NQRETAVVWDKATGQPIYNAIVWQSRQTKDICTQLKEAGHEQMVRDKTGLLIDAYFSGTKVKWILDHVDGARERARKGEL
AFGTIDSWLIWNLTGGKVHVTDYTNASRTMMYNIHTLEWDAELLEMLDVPAQMLPEVRSSSEVYGMTQTQYFYGEQVPIA
GIAGDQQAALFGQACFEPGIAKNTYGTGCFMLMNTGDKAVASKAGLLTTIAWGIDGKVEYALEGAIFVAGSVVQWLRDGL
RMFGKASDSQAYAERAGDNDGVYFVPAFVGLGAPYWRSDIRGAVFGLTRGTSKEHFVRAAVESMAYQTRDVLTAMQSDSG
IELKELRADGGAIANDFMAQFQSDILNVPVLRPEVAETTALGAAYLAGLATGFWSSREEIAKQWAVDRRFEPNMPEERRE
QLYAGWQQAVEATMGFRIS

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=512, Percent_Identity=46.6796875, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI4504007, Length=512, Percent_Identity=46.6796875, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI42794763, Length=516, Percent_Identity=46.3178294573643, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI41393575, Length=505, Percent_Identity=45.7425742574257, Blast_Score=448, Evalue=1e-126,
Organism=Homo sapiens, GI88196792, Length=509, Percent_Identity=32.2200392927308, Blast_Score=301, Evalue=1e-81,
Organism=Escherichia coli, GI1790361, Length=495, Percent_Identity=61.6161616161616, Blast_Score=658, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=524, Percent_Identity=23.6641221374046, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1789168, Length=495, Percent_Identity=23.8383838383838, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1787789, Length=524, Percent_Identity=23.8549618320611, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1789987, Length=492, Percent_Identity=23.780487804878, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535599, Length=500, Percent_Identity=40.8, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71986140, Length=526, Percent_Identity=24.9049429657795, Blast_Score=185, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI71986147, Length=267, Percent_Identity=31.0861423220974, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321755, Length=549, Percent_Identity=36.247723132969, Blast_Score=305, Evalue=9e-84,
Organism=Drosophila melanogaster, GI24655555, Length=504, Percent_Identity=40.2777777777778, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655560, Length=504, Percent_Identity=40.2777777777778, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655570, Length=504, Percent_Identity=40.2777777777778, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655574, Length=504, Percent_Identity=40.2777777777778, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655565, Length=504, Percent_Identity=40.2777777777778, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17864214, Length=514, Percent_Identity=41.8287937743191, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24654573, Length=437, Percent_Identity=43.4782608695652, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI19922058, Length=525, Percent_Identity=34.0952380952381, Blast_Score=295, Evalue=5e-80,
Organism=Drosophila melanogaster, GI24652871, Length=491, Percent_Identity=34.826883910387, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI24656367, Length=492, Percent_Identity=29.6747967479675, Blast_Score=231, Evalue=7e-61,
Organism=Drosophila melanogaster, GI21357867, Length=507, Percent_Identity=28.2051282051282, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24656372, Length=331, Percent_Identity=31.7220543806647, Blast_Score=175, Evalue=8e-44,
Organism=Drosophila melanogaster, GI45552909, Length=292, Percent_Identity=29.1095890410959, Blast_Score=132, Evalue=5e-31,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55230; Mature: 55230

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKYVLAIDQGTTSSRAMLFDRQGKVAGVAQREFGQIFPQPGWVEHNPREIMTSVYTTI
CCCCEEEEEECCCCCCCEEEEECCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
TELLNNAQIDAREISGIGITNQRETAVVWDKATGQPIYNAIVWQSRQTKDICTQLKEAGH
HHHHCCCCCCHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHCCH
EQMVRDKTGLLIDAYFSGTKVKWILDHVDGARERARKGELAFGTIDSWLIWNLTGGKVHV
HHHHHHCCCCEEEEEECCCEEEEEEHHCCCHHHHHHCCCEEEEECCCEEEEEECCCEEEE
TDYTNASRTMMYNIHTLEWDAELLEMLDVPAQMLPEVRSSSEVYGMTQTQYFYGEQVPIA
EECCCCCCEEEEEEEEECCCHHHHHHHCCCHHHHHHHHCCCCEECCEEEEEECCCCCCEE
GIAGDQQAALFGQACFEPGIAKNTYGTGCFMLMNTGDKAVASKAGLLTTIAWGIDGKVEY
EECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHCCHHEEEEECCCCEEEE
ALEGAIFVAGSVVQWLRDGLRMFGKASDSQAYAERAGDNDGVYFVPAFVGLGAPYWRSDI
EECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEHHHHCCCHHHHHHC
RGAVFGLTRGTSKEHFVRAAVESMAYQTRDVLTAMQSDSGIELKELRADGGAIANDFMAQ
CHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHH
FQSDILNVPVLRPEVAETTALGAAYLAGLATGFWSSREEIAKQWAVDRRFEPNMPEERRE
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHH
QLYAGWQQAVEATMGFRIS
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEKKYVLAIDQGTTSSRAMLFDRQGKVAGVAQREFGQIFPQPGWVEHNPREIMTSVYTTI
CCCCEEEEEECCCCCCCEEEEECCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
TELLNNAQIDAREISGIGITNQRETAVVWDKATGQPIYNAIVWQSRQTKDICTQLKEAGH
HHHHCCCCCCHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHCCH
EQMVRDKTGLLIDAYFSGTKVKWILDHVDGARERARKGELAFGTIDSWLIWNLTGGKVHV
HHHHHHCCCCEEEEEECCCEEEEEEHHCCCHHHHHHCCCEEEEECCCEEEEEECCCEEEE
TDYTNASRTMMYNIHTLEWDAELLEMLDVPAQMLPEVRSSSEVYGMTQTQYFYGEQVPIA
EECCCCCCEEEEEEEEECCCHHHHHHHCCCHHHHHHHHCCCCEECCEEEEEECCCCCCEE
GIAGDQQAALFGQACFEPGIAKNTYGTGCFMLMNTGDKAVASKAGLLTTIAWGIDGKVEY
EECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHCCHHEEEEECCCCEEEE
ALEGAIFVAGSVVQWLRDGLRMFGKASDSQAYAERAGDNDGVYFVPAFVGLGAPYWRSDI
EECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEHHHHCCCHHHHHHC
RGAVFGLTRGTSKEHFVRAAVESMAYQTRDVLTAMQSDSGIELKELRADGGAIANDFMAQ
CHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHH
FQSDILNVPVLRPEVAETTALGAAYLAGLATGFWSSREEIAKQWAVDRRFEPNMPEERRE
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHH
QLYAGWQQAVEATMGFRIS
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]