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The map label for this gene is puuD [H]

Identifier: 66045942

GI number: 66045942

Start: 3287200

End: 3287949

Strand: Reverse

Name: puuD [H]

Synonym: Psyr_2706

Alternate gene names: 66045942

Gene position: 3287949-3287200 (Counterclockwise)

Preceding gene: 66045946

Following gene: 66045941

Centisome position: 53.96

GC content: 64.67

Gene sequence:

>750_bases
ATGTCGCGACCTCCCTTGATCGGTGTCACCGCCTGCCGTCAGCAGTTGGGCAAGTATTCTTCTCACACCGCAGGCGACAA
GTACGTCGAGGCTGCAGCCTTCGCCGGCATGCCGGTGATTCTGCCTGCACTCGAGGTGCCCACCGAGCCTGCGCAACTGC
TCGCGTCGCTGGATGGCCTGCTGTTTACCGGCTCGCCGTCCAACGTCGAGCCGCGTCACTACAATGGACCGGCGAGCGTG
GAGGGCACCGCTCATGATGTATTCCGGGACCGCACCACACTGCCGCTGCTCAGGGCTGCCATCGCTCAGGGCGTGCCGGT
GTTGTGCATTTGCCGCGGTTTTCAGGAGCTGAATGTCGCTTTGGGCGGCAGCCTGCATCAGCGGGTTCAGGAGTTGCCCG
GCTATCTGGATCATCGTGAGCCGCAAAGCGAGGTGCTGGCGGTGCAGTACACGCCAAAGCATGCCGTGCAGGTGCAGCCT
GGCGGTTTGCTCGACTCGCTCGGGCTGGGCCCGGGCTTCGAGGTCAACAGCCTGCACAGTCAGGGTGTCGATCGTCTGGC
GTCGGACCTGCGCGCAGAAGCATTGGCCCCCGACGGTCTGGTCGAAGCGGTGTCCATGCCCGGCGCGCCAGGCTTTGTGC
TGGGTGTGCAGTGGCATCCGGAATGGGAGTTCATGGACAACCCCGTGTCGCTCAGCCTGTTCAAGGCCTTCCGCGAGGCC
TGCCAGAGCTATGCGCGCAACCGCCGCTAG

Upstream 100 bases:

>100_bases
CTATGTTTGTTGCGCAGATGACTATCCTAGACTGGTTTCATTCCCGCTGGATAGCGTCCAAATGGCGCTGACCATTCAGA
TTTCACACAGGATTCAAGCT

Downstream 100 bases:

>100_bases
AGCAATGAGCTGCTGAGTGTTGCGGGCAAACGTGATTTAATATCCGGCTTGACGGCTTCGGGTAATGGACGATCCATTGC
GCCGGGGCGCTGCCTCATTC

Product: glutamine amidotransferase

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSRPPLIGVTACRQQLGKYSSHTAGDKYVEAAAFAGMPVILPALEVPTEPAQLLASLDGLLFTGSPSNVEPRHYNGPASV
EGTAHDVFRDRTTLPLLRAAIAQGVPVLCICRGFQELNVALGGSLHQRVQELPGYLDHREPQSEVLAVQYTPKHAVQVQP
GGLLDSLGLGPGFEVNSLHSQGVDRLASDLRAEALAPDGLVEAVSMPGAPGFVLGVQWHPEWEFMDNPVSLSLFKAFREA
CQSYARNRR

Sequences:

>Translated_249_residues
MSRPPLIGVTACRQQLGKYSSHTAGDKYVEAAAFAGMPVILPALEVPTEPAQLLASLDGLLFTGSPSNVEPRHYNGPASV
EGTAHDVFRDRTTLPLLRAAIAQGVPVLCICRGFQELNVALGGSLHQRVQELPGYLDHREPQSEVLAVQYTPKHAVQVQP
GGLLDSLGLGPGFEVNSLHSQGVDRLASDLRAEALAPDGLVEAVSMPGAPGFVLGVQWHPEWEFMDNPVSLSLFKAFREA
CQSYARNRR
>Mature_248_residues
SRPPLIGVTACRQQLGKYSSHTAGDKYVEAAAFAGMPVILPALEVPTEPAQLLASLDGLLFTGSPSNVEPRHYNGPASVE
GTAHDVFRDRTTLPLLRAAIAQGVPVLCICRGFQELNVALGGSLHQRVQELPGYLDHREPQSEVLAVQYTPKHAVQVQPG
GLLDSLGLGPGFEVNSLHSQGVDRLASDLRAEALAPDGLVEAVSMPGAPGFVLGVQWHPEWEFMDNPVSLSLFKAFREAC
QSYARNRR

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=248, Percent_Identity=45.5645161290323, Blast_Score=188, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 26781; Mature: 26650

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPPLIGVTACRQQLGKYSSHTAGDKYVEAAAFAGMPVILPALEVPTEPAQLLASLDGL
CCCCCEECHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEHHHCCCCCHHHHHHHHCCE
LFTGSPSNVEPRHYNGPASVEGTAHDVFRDRTTLPLLRAAIAQGVPVLCICRGFQELNVA
EEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCEEEHHHCHHHHHHH
LGGSLHQRVQELPGYLDHREPQSEVLAVQYTPKHAVQVQPGGLLDSLGLGPGFEVNSLHS
HCCHHHHHHHHCCCHHCCCCCCCCEEEEEECCCCEEEECCCCCHHHCCCCCCCCCHHHHH
QGVDRLASDLRAEALAPDGLVEAVSMPGAPGFVLGVQWHPEWEFMDNPVSLSLFKAFREA
HHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHH
CQSYARNRR
HHHHHHCCC
>Mature Secondary Structure 
SRPPLIGVTACRQQLGKYSSHTAGDKYVEAAAFAGMPVILPALEVPTEPAQLLASLDGL
CCCCEECHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEHHHCCCCCHHHHHHHHCCE
LFTGSPSNVEPRHYNGPASVEGTAHDVFRDRTTLPLLRAAIAQGVPVLCICRGFQELNVA
EEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCEEEHHHCHHHHHHH
LGGSLHQRVQELPGYLDHREPQSEVLAVQYTPKHAVQVQPGGLLDSLGLGPGFEVNSLHS
HCCHHHHHHHHCCCHHCCCCCCCCEEEEEECCCCEEEECCCCCHHHCCCCCCCCCHHHHH
QGVDRLASDLRAEALAPDGLVEAVSMPGAPGFVLGVQWHPEWEFMDNPVSLSLFKAFREA
HHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHH
CQSYARNRR
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]