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The map label for this gene is pcaQ [H]

Identifier: 66045361

GI number: 66045361

Start: 2478901

End: 2479821

Strand: Direct

Name: pcaQ [H]

Synonym: Psyr_2121

Alternate gene names: 66045361

Gene position: 2478901-2479821 (Clockwise)

Preceding gene: 66045360

Following gene: 66045362

Centisome position: 40.68

GC content: 62.21

Gene sequence:

>921_bases
GTGAATATCGATACACGCATCAAATTTCGCCATCTGGTCTGCTTTCTCGAAGTGGCCCGGCAGGGCAGCCTGGCGCGTGC
CTCCGACGTTCTGGCGATCAGTCAGCCTGCGTTGTCGAAAAGTCTCAAAGAGCTGGAAACCCTGCTCGACACCACGTTGT
TCGTGCGCAGCAAGAGCGGTGCGGCACTTACCGAAGCGGGTGTGGCGTTCATGCGCTTTGCCGGGCCGAGCGTTCAGGCC
CTGCGCGAGGGTGTCAGCAGCCTGAGATCCGGTGAACATGACACCGTTACTGCACGGCTGGGCGTACTGTCGACGGCCGA
AAGCCTGCTGGTGCCTGAGGTCGTTCATCGCCTGCACCAGCGGCACCCGGCGCTGATCGTCAGCGTGATGACCGGGCCCA
GTGCCTATCTGCTGTCGCAATTGAGGGTAGGGGAGCTGGATCTGGTGGTCGGGCGCATGACTGACAGCCCGCAGATTCAG
GGCCTGACGTTCGAGCACCTGTACAACGAGTCGATGACACTGGTGGTGCGCAGCGATCACCCGCTGCTGGCTGCGCCGCT
CAAGCCCGAAAGCCTGGAGCAATTCCCGCTGGTGTTGCCGCTGGCAGGCACGACCATCCGCAAGTTTGCCGACAGCCTGT
TCGTGCAGTGCGGTATTCGCATGCCGCGGCAACGCCTGGAAACCCTCTCGCTTACCCTCAGTCGGCGCTATGTGCAATGC
AGCGATGCGGTGTGGATCGCGCCACTGGATGCCGTGTCGCTGGAGTTGAAAGGTGGCACGCTGGTTGAACTGGACATGGG
TATTCGTGAGCCGGGCGGCTCGGTCGGTTTGTGCAGCAACCCGGCCTTGCCACTCACACGCGCAGCACAGTGGTGCGTGG
ACGAGCTGCGCAGTCTTGGAGAAGCCTGTCGCAAAGTCTAG

Upstream 100 bases:

>100_bases
GAATGGCCATTTGACGCGCCTCAAGTGGCGTCGTTTCTGAGTCGCTGAGCTGTCTTGTCGTTGAGTTGCATTCCCTTTCA
TTTACCGGACACGACGCGCA

Downstream 100 bases:

>100_bases
CATCCATAACCAAATGGTTATGGATCATGGGGTTTATTTCAGTTCTCTATAAGCATGGATCGCGCGACACTTCGCCGCAG
CCTGTAGCGCATGTGATCGA

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: Pca operon transcriptional activator [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MNIDTRIKFRHLVCFLEVARQGSLARASDVLAISQPALSKSLKELETLLDTTLFVRSKSGAALTEAGVAFMRFAGPSVQA
LREGVSSLRSGEHDTVTARLGVLSTAESLLVPEVVHRLHQRHPALIVSVMTGPSAYLLSQLRVGELDLVVGRMTDSPQIQ
GLTFEHLYNESMTLVVRSDHPLLAAPLKPESLEQFPLVLPLAGTTIRKFADSLFVQCGIRMPRQRLETLSLTLSRRYVQC
SDAVWIAPLDAVSLELKGGTLVELDMGIREPGGSVGLCSNPALPLTRAAQWCVDELRSLGEACRKV

Sequences:

>Translated_306_residues
MNIDTRIKFRHLVCFLEVARQGSLARASDVLAISQPALSKSLKELETLLDTTLFVRSKSGAALTEAGVAFMRFAGPSVQA
LREGVSSLRSGEHDTVTARLGVLSTAESLLVPEVVHRLHQRHPALIVSVMTGPSAYLLSQLRVGELDLVVGRMTDSPQIQ
GLTFEHLYNESMTLVVRSDHPLLAAPLKPESLEQFPLVLPLAGTTIRKFADSLFVQCGIRMPRQRLETLSLTLSRRYVQC
SDAVWIAPLDAVSLELKGGTLVELDMGIREPGGSVGLCSNPALPLTRAAQWCVDELRSLGEACRKV
>Mature_306_residues
MNIDTRIKFRHLVCFLEVARQGSLARASDVLAISQPALSKSLKELETLLDTTLFVRSKSGAALTEAGVAFMRFAGPSVQA
LREGVSSLRSGEHDTVTARLGVLSTAESLLVPEVVHRLHQRHPALIVSVMTGPSAYLLSQLRVGELDLVVGRMTDSPQIQ
GLTFEHLYNESMTLVVRSDHPLLAAPLKPESLEQFPLVLPLAGTTIRKFADSLFVQCGIRMPRQRLETLSLTLSRRYVQC
SDAVWIAPLDAVSLELKGGTLVELDMGIREPGGSVGLCSNPALPLTRAAQWCVDELRSLGEACRKV

Specific function: Activates transcription of the pcaDCHGB operon for the catabolism of the phenolic compound protocatechuate [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081904, Length=293, Percent_Identity=35.8361774744027, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1787601, Length=181, Percent_Identity=35.3591160220994, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1788887, Length=187, Percent_Identity=29.9465240641711, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI157672245, Length=179, Percent_Identity=30.7262569832402, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1790399, Length=296, Percent_Identity=25.6756756756757, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789506, Length=194, Percent_Identity=27.319587628866, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788748, Length=217, Percent_Identity=26.2672811059908, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788296, Length=223, Percent_Identity=26.9058295964126, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788297, Length=214, Percent_Identity=26.6355140186916, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR012787
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33330; Mature: 33330

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIDTRIKFRHLVCFLEVARQGSLARASDVLAISQPALSKSLKELETLLDTTLFVRSKSG
CCCCCHHHHHHHHHHHHHHHCCCCHHHHCHHEECCHHHHHHHHHHHHHHHHHHEEECCCC
AALTEAGVAFMRFAGPSVQALREGVSSLRSGEHDTVTARLGVLSTAESLLVPEVVHRLHQ
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHC
RHPALIVSVMTGPSAYLLSQLRVGELDLVVGRMTDSPQIQGLTFEHLYNESMTLVVRSDH
CCCEEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCCCEEHHHHHCCCEEEEEECCC
PLLAAPLKPESLEQFPLVLPLAGTTIRKFADSLFVQCGIRMPRQRLETLSLTLSRRYVQC
CEEECCCCCCCHHHCCEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEE
SDAVWIAPLDAVSLELKGGTLVELDMGIREPGGSVGLCSNPALPLTRAAQWCVDELRSLG
CCCEEEEECCEEEEEECCCEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHH
EACRKV
HHHHCC
>Mature Secondary Structure
MNIDTRIKFRHLVCFLEVARQGSLARASDVLAISQPALSKSLKELETLLDTTLFVRSKSG
CCCCCHHHHHHHHHHHHHHHCCCCHHHHCHHEECCHHHHHHHHHHHHHHHHHHEEECCCC
AALTEAGVAFMRFAGPSVQALREGVSSLRSGEHDTVTARLGVLSTAESLLVPEVVHRLHQ
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHC
RHPALIVSVMTGPSAYLLSQLRVGELDLVVGRMTDSPQIQGLTFEHLYNESMTLVVRSDH
CCCEEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCCCEEHHHHHCCCEEEEEECCC
PLLAAPLKPESLEQFPLVLPLAGTTIRKFADSLFVQCGIRMPRQRLETLSLTLSRRYVQC
CEEECCCCCCCHHHCCEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEE
SDAVWIAPLDAVSLELKGGTLVELDMGIREPGGSVGLCSNPALPLTRAAQWCVDELRSLG
CCCEEEEECCEEEEEECCCEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHH
EACRKV
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]