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The map label for this gene is rlmL [H]

Identifier: 66045348

GI number: 66045348

Start: 2460168

End: 2462420

Strand: Direct

Name: rlmL [H]

Synonym: Psyr_2108

Alternate gene names: 66045348

Gene position: 2460168-2462420 (Clockwise)

Preceding gene: 66045346

Following gene: 66045349

Centisome position: 40.37

GC content: 61.56

Gene sequence:

>2253_bases
ATGTCGGATCGCTACGAACTTTTCCTCACTTGCCCCAAAGGCCTTGAAGGCCTGCTTGCCGAGGAAGCCACTGCGCTGGG
TCTGCAAGAGACCCGTGAACACACCTCAGCCATTCGCGGCTCGGCGGACATGGAGACCGCTTACCGGCTGTGTCTCTGGT
CGCGCCTGGCCAACCGCGTGTTGCTGGTGCTCAAGCGCTTCCCGATGAAGGATGCCGAAGACCTTTACCACGGCGTGCTG
GATGTCGAGTGGCAGGACCATCTCGAATCCGACGGCACCATTGCGGTGGAATTCAGTGGTCATGGTTCGGGCATCGACAA
CACCCATTTCGGTGCACTGAAGGTCAAGGATGCGATCGTCGACAAGCTGCGCACCCCGGATGGCGAGCGTCCGTCGGTCG
ACAAGATCAACCCCGATCTGCGCGTGCACCTGCGTCTGGATCGCGGCGAGGCCATTCTGTCCCTCGACCTTTCCGGGCAC
AGCCTGCACCAGCGCGGTTACCGTCTGCAGCAGGGCGCTGCACCGCTGAAGGAAAACCTCGCCGCCGCCATCCTGATCCG
CGCTGGCTGGCCGCGTATTGCTGCCGAGGGCGGCGCGCTGGCTGACCCGATGTGCGGTGTCGGTACCTTTCTGGTCGAGG
CCGGGATGATCGCCGCCGACATCGCGCCCAACATCAAGCGTGAACGCTGGGGCTTCTCGGCCTGGCTGGGCCACGTCCCG
GCGCTGTGGCGCAAGCTGCACGACGAAGCGCTGGCCCGCGCCGAGGCCGGGTTGGCCAAAACACCTTCGTGGATTCGCGG
TTACGAAGCCGATCCTCGACTGATCCAGCCTGGGCGCAATAATATCGAGCGTGCCGGCCTGAGCGACTGGATCAAGGTCT
ATCAGGGCGAAGTCGCGACCTTCGAGCCGCGTCCCGATCAGAATCAGAAAGGCCTGGTGATCTGCAACCCTCCGTACGGC
GAGCGTCTGGGAGATGAAGCCAGTCTGCTGTATCTCTACCAGAACCTTGGCGAGCGCCTGCGTCAGGCGTGCCTTAACTG
GGAAGCTGCGGTGTTCACCGGCGCGCCGGACCTGGGCAAGCGCATGGGCATTCGCAGCCACAAACAGTATTCGTTCTGGA
ACGGCGCCTTGCCGTGCAAGCTGCTGCTGATCAAGGTCACGCCTGATCAGTTCGTGACCGGCGAGCGACGCACCCCCGAG
CAGCGTCAGATCGAACGCGAAAACCCTGTCGAGGTTGAAGTCGTTGAGCGCAAACTCAACAAGAACGGCAACCCGATCAA
GCCGGAACCGGTGGTGGTCGAGCAGGCGCGCCTGAGCGAAGGCGGGCAGATGTTTGCCAATCGTCTGCAAAAGAACCTCA
AGCTGATGGGCAAGTGGGTGCGTCGCGAAGGGATCGATTGCTACCGCGTGTATGACGCCGACATGCCTGAATACTCGCTG
GCGATCGATCTGTATCACGACTGGGTGCATGTTCAGGAATACGCAGCACCCAAGTCCATTGATCCGGAAAAGGCGTCAGC
GCGGCTGTTCGATGCGCTGGCGGCCATTCCGCAAGCACTGAACATCGACAAGAACCGCGTGGTGATCAAGCGTCGCGAGC
GTCAGAGCGGCACCAAGCAGTACGAGCGTCAAAGCGCCCAGGGCCAGTTTCTGGAAGTCAGCGAAGGCGGCGTCAAGCTG
CTGGTCAATCTGACCGACTACCTGGACACCGGGCTGTTTCTCGATCACCGGCCGATGCGCATGCGTATTCAGCGCGAGGC
GTCCGGCAAGCGCTTCCTGAACCTGTTTGCCTATACGGCGACGGCCAGTGTGCATGCTGCCAAGGGCGGTGCGCGCAGCA
CCACCAGCGTCGACCTGTCGCGGACCTATCTGGACTGGGCGCGTCGTAACCTGTCGCTGAACGGTTTCTCCGACAAGAAT
CGTCTGGAGCAGGGCGACGTGATGGCCTGGCTGCAGGCCAACCGCGACGAGTACGACCTGATCTTCATCGACCCGCCGAC
GTTCTCCAACTCCAAGCGCATGGAAGGCATTTTTGATGTCCAGCGTGATCAGGTCGAGCTGATCGATCTGGCCATGGCGC
GTCTGGCGCCGGGTGGCGTGTTGTACTTCTCCAACAACTTCCGCAAGTTTGTGCTCGATGAAAACCTGAGTCAGCGTTAT
GCGGTCGAGGACATTACCGCGCACACCATCGACCAGGACTTTGCCCGTAACGGCAAGATCCACCGCGCCTGGAAAATCAT
GGCGCGTAGCTGA

Upstream 100 bases:

>100_bases
ACATCACGTGCCACGCAGGCGTTCTCCATGAAAGACGCCTGTTCAAATCTGGCAGGGCAATGCGTTGCCCGCCGCAGACG
GCTCAGGCCCTGGAATACAC

Downstream 100 bases:

>100_bases
TCCGGTGCGCGATGAACCGATCGGTAACGGTCCGGTCATCGCGCCTCGCGACACTTCCTGCACTCGTTTGCATGCCCGTG
TGCTCAAACGCGTTTGAGCC

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 750; Mature: 749

Protein sequence:

>750_residues
MSDRYELFLTCPKGLEGLLAEEATALGLQETREHTSAIRGSADMETAYRLCLWSRLANRVLLVLKRFPMKDAEDLYHGVL
DVEWQDHLESDGTIAVEFSGHGSGIDNTHFGALKVKDAIVDKLRTPDGERPSVDKINPDLRVHLRLDRGEAILSLDLSGH
SLHQRGYRLQQGAAPLKENLAAAILIRAGWPRIAAEGGALADPMCGVGTFLVEAGMIAADIAPNIKRERWGFSAWLGHVP
ALWRKLHDEALARAEAGLAKTPSWIRGYEADPRLIQPGRNNIERAGLSDWIKVYQGEVATFEPRPDQNQKGLVICNPPYG
ERLGDEASLLYLYQNLGERLRQACLNWEAAVFTGAPDLGKRMGIRSHKQYSFWNGALPCKLLLIKVTPDQFVTGERRTPE
QRQIERENPVEVEVVERKLNKNGNPIKPEPVVVEQARLSEGGQMFANRLQKNLKLMGKWVRREGIDCYRVYDADMPEYSL
AIDLYHDWVHVQEYAAPKSIDPEKASARLFDALAAIPQALNIDKNRVVIKRRERQSGTKQYERQSAQGQFLEVSEGGVKL
LVNLTDYLDTGLFLDHRPMRMRIQREASGKRFLNLFAYTATASVHAAKGGARSTTSVDLSRTYLDWARRNLSLNGFSDKN
RLEQGDVMAWLQANRDEYDLIFIDPPTFSNSKRMEGIFDVQRDQVELIDLAMARLAPGGVLYFSNNFRKFVLDENLSQRY
AVEDITAHTIDQDFARNGKIHRAWKIMARS

Sequences:

>Translated_750_residues
MSDRYELFLTCPKGLEGLLAEEATALGLQETREHTSAIRGSADMETAYRLCLWSRLANRVLLVLKRFPMKDAEDLYHGVL
DVEWQDHLESDGTIAVEFSGHGSGIDNTHFGALKVKDAIVDKLRTPDGERPSVDKINPDLRVHLRLDRGEAILSLDLSGH
SLHQRGYRLQQGAAPLKENLAAAILIRAGWPRIAAEGGALADPMCGVGTFLVEAGMIAADIAPNIKRERWGFSAWLGHVP
ALWRKLHDEALARAEAGLAKTPSWIRGYEADPRLIQPGRNNIERAGLSDWIKVYQGEVATFEPRPDQNQKGLVICNPPYG
ERLGDEASLLYLYQNLGERLRQACLNWEAAVFTGAPDLGKRMGIRSHKQYSFWNGALPCKLLLIKVTPDQFVTGERRTPE
QRQIERENPVEVEVVERKLNKNGNPIKPEPVVVEQARLSEGGQMFANRLQKNLKLMGKWVRREGIDCYRVYDADMPEYSL
AIDLYHDWVHVQEYAAPKSIDPEKASARLFDALAAIPQALNIDKNRVVIKRRERQSGTKQYERQSAQGQFLEVSEGGVKL
LVNLTDYLDTGLFLDHRPMRMRIQREASGKRFLNLFAYTATASVHAAKGGARSTTSVDLSRTYLDWARRNLSLNGFSDKN
RLEQGDVMAWLQANRDEYDLIFIDPPTFSNSKRMEGIFDVQRDQVELIDLAMARLAPGGVLYFSNNFRKFVLDENLSQRY
AVEDITAHTIDQDFARNGKIHRAWKIMARS
>Mature_749_residues
SDRYELFLTCPKGLEGLLAEEATALGLQETREHTSAIRGSADMETAYRLCLWSRLANRVLLVLKRFPMKDAEDLYHGVLD
VEWQDHLESDGTIAVEFSGHGSGIDNTHFGALKVKDAIVDKLRTPDGERPSVDKINPDLRVHLRLDRGEAILSLDLSGHS
LHQRGYRLQQGAAPLKENLAAAILIRAGWPRIAAEGGALADPMCGVGTFLVEAGMIAADIAPNIKRERWGFSAWLGHVPA
LWRKLHDEALARAEAGLAKTPSWIRGYEADPRLIQPGRNNIERAGLSDWIKVYQGEVATFEPRPDQNQKGLVICNPPYGE
RLGDEASLLYLYQNLGERLRQACLNWEAAVFTGAPDLGKRMGIRSHKQYSFWNGALPCKLLLIKVTPDQFVTGERRTPEQ
RQIERENPVEVEVVERKLNKNGNPIKPEPVVVEQARLSEGGQMFANRLQKNLKLMGKWVRREGIDCYRVYDADMPEYSLA
IDLYHDWVHVQEYAAPKSIDPEKASARLFDALAAIPQALNIDKNRVVIKRRERQSGTKQYERQSAQGQFLEVSEGGVKLL
VNLTDYLDTGLFLDHRPMRMRIQREASGKRFLNLFAYTATASVHAAKGGARSTTSVDLSRTYLDWARRNLSLNGFSDKNR
LEQGDVMAWLQANRDEYDLIFIDPPTFSNSKRMEGIFDVQRDQVELIDLAMARLAPGGVLYFSNNFRKFVLDENLSQRYA
VEDITAHTIDQDFARNGKIHRAWKIMARS

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=746, Percent_Identity=44.5040214477212, Blast_Score=593, Evalue=1e-171,
Organism=Escherichia coli, GI145693111, Length=286, Percent_Identity=29.3706293706294, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 84694; Mature: 84563

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRYELFLTCPKGLEGLLAEEATALGLQETREHTSAIRGSADMETAYRLCLWSRLANRV
CCCCEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LLVLKRFPMKDAEDLYHGVLDVEWQDHLESDGTIAVEFSGHGSGIDNTHFGALKVKDAIV
HHHHHHCCCCCHHHHHHCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEHHHHHH
DKLRTPDGERPSVDKINPDLRVHLRLDRGEAILSLDLSGHSLHQRGYRLQQGAAPLKENL
HHHCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHCCCCHHCCCCCHHHCC
AAAILIRAGWPRIAAEGGALADPMCGVGTFLVEAGMIAADIAPNIKRERWGFSAWLGHVP
EEEEEEECCCCCEECCCCCCCCCHHHHHHHHHHCCCCEECCCCCCHHHHCCHHHHHHHHH
ALWRKLHDEALARAEAGLAKTPSWIRGYEADPRLIQPGRNNIERAGLSDWIKVYQGEVAT
HHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCEECCCCCCHHHCCHHHHHHHHCCCEEE
FEPRPDQNQKGLVICNPPYGERLGDEASLLYLYQNLGERLRQACLNWEAAVFTGAPDLGK
ECCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
RMGIRSHKQYSFWNGALPCKLLLIKVTPDQFVTGERRTPEQRQIERENPVEVEVVERKLN
HCCCCCCCCCCCCCCCCCEEEEEEEECCCHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHC
KNGNPIKPEPVVVEQARLSEGGQMFANRLQKNLKLMGKWVRREGIDCYRVYDADMPEYSL
CCCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEE
AIDLYHDWVHVQEYAAPKSIDPEKASARLFDALAAIPQALNIDKNRVVIKRRERQSGTKQ
EEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCHHHH
YERQSAQGQFLEVSEGGVKLLVNLTDYLDTGLFLDHRPMRMRIQREASGKRFLNLFAYTA
HHHHCCCCCEEEECCCCEEEEEEEHHHHCCCCEECCCCHHEEEEHHCCCHHHHHHHHHHH
TASVHAAKGGARSTTSVDLSRTYLDWARRNLSLNGFSDKNRLEQGDVMAWLQANRDEYDL
HCEEEECCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCEEE
IFIDPPTFSNSKRMEGIFDVQRDQVELIDLAMARLAPGGVLYFSNNFRKFVLDENLSQRY
EEECCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCHHH
AVEDITAHTIDQDFARNGKIHRAWKIMARS
HHHHHHHHHHHHHHHHCCCEEEEEEEEECC
>Mature Secondary Structure 
SDRYELFLTCPKGLEGLLAEEATALGLQETREHTSAIRGSADMETAYRLCLWSRLANRV
CCCEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LLVLKRFPMKDAEDLYHGVLDVEWQDHLESDGTIAVEFSGHGSGIDNTHFGALKVKDAIV
HHHHHHCCCCCHHHHHHCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEHHHHHH
DKLRTPDGERPSVDKINPDLRVHLRLDRGEAILSLDLSGHSLHQRGYRLQQGAAPLKENL
HHHCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHCCCCHHCCCCCHHHCC
AAAILIRAGWPRIAAEGGALADPMCGVGTFLVEAGMIAADIAPNIKRERWGFSAWLGHVP
EEEEEEECCCCCEECCCCCCCCCHHHHHHHHHHCCCCEECCCCCCHHHHCCHHHHHHHHH
ALWRKLHDEALARAEAGLAKTPSWIRGYEADPRLIQPGRNNIERAGLSDWIKVYQGEVAT
HHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCEECCCCCCHHHCCHHHHHHHHCCCEEE
FEPRPDQNQKGLVICNPPYGERLGDEASLLYLYQNLGERLRQACLNWEAAVFTGAPDLGK
ECCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
RMGIRSHKQYSFWNGALPCKLLLIKVTPDQFVTGERRTPEQRQIERENPVEVEVVERKLN
HCCCCCCCCCCCCCCCCCEEEEEEEECCCHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHC
KNGNPIKPEPVVVEQARLSEGGQMFANRLQKNLKLMGKWVRREGIDCYRVYDADMPEYSL
CCCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEE
AIDLYHDWVHVQEYAAPKSIDPEKASARLFDALAAIPQALNIDKNRVVIKRRERQSGTKQ
EEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCHHHH
YERQSAQGQFLEVSEGGVKLLVNLTDYLDTGLFLDHRPMRMRIQREASGKRFLNLFAYTA
HHHHCCCCCEEEECCCCEEEEEEEHHHHCCCCEECCCCHHEEEEHHCCCHHHHHHHHHHH
TASVHAAKGGARSTTSVDLSRTYLDWARRNLSLNGFSDKNRLEQGDVMAWLQANRDEYDL
HCEEEECCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCEEE
IFIDPPTFSNSKRMEGIFDVQRDQVELIDLAMARLAPGGVLYFSNNFRKFVLDENLSQRY
EEECCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCHHH
AVEDITAHTIDQDFARNGKIHRAWKIMARS
HHHHHHHHHHHHHHHHCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA