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The map label for this gene is cysB [H]

Identifier: 66045317

GI number: 66045317

Start: 2422181

End: 2423155

Strand: Direct

Name: cysB [H]

Synonym: Psyr_2077

Alternate gene names: 66045317

Gene position: 2422181-2423155 (Clockwise)

Preceding gene: 66045316

Following gene: 66045319

Centisome position: 39.75

GC content: 59.18

Gene sequence:

>975_bases
ATGAAGCTCCAACAACTGCGCTACATTTGGGAAGTGGCGCACCACGACCTCAACGTTTCCGCGACGGCCCAGAGCCTTTA
CACCTCCCAGCCCGGCATCAGCAAGCAGATCCGTCTGCTTGAAGATGAACTGGGCGTCGAGGTTTTCGCCCGCAGCGGCA
AGCACCTGACGCGTGTGACACCTGCAGGCGAGCGCATCATCACGACAGCGGGCGAGATCCTGCGCAAGGTCGAGAGCATC
AAGCAGATTGCCCAGGAGTTCTCCAACGAGAAGAAGGGCACGCTGTCGATCGCCACCACCCACACCCAGGCGCGCTATGC
CTTGCCGTCGGTGATCAGCAACTTCATCAAGCAATACCCTGATGTTGCGCTGCACATGCACCAGGGCTCACCGATGCAGA
TCGCCGAAATGGCCGCTGACGGGACGGTCGATTTCGCTATCGCCACCGAGGCGCTCGAGCAGTTCAGCGATCTGGTGATG
ATGCCGTGCTATCGCTGGAACCGTTGCGTTGTCGTGCCACAGGGCCACCCGTTGACCAAGGCACCCAAGCTGACCCTGGA
ACTGCTGGCCGAATACCCGATCGTGACTTACGTCTTCGGTTTCACCGGTCGTTCCAAGCTGGATGAGGCGTTCAGCCATC
GCGGGCTGGTGCCCAAGGTGGTGTTTACGGCGGCGGACGCCGACGTGATCAAGACCTACGTCAGGCTGAATCTGGGTGTG
GGTATCGTGGCGAGAATGGCGGTAGACGAGGAAGTGGACAGCGACCTGGTGGTGCTCGATGCCAGTGATCTGTTCGAGTC
CAGCGTTACAAAAATCGCTTTCCGTCGCGGCACTTTCCTGCGGGGCTTCATGTGCGATTTCATCGAGAAATTCGCGCCGC
ACCTGACCCGCGAAGTCATGGCCAAGGCGATTCAGTGCCATAACAAGCAGGAAATGGAAGAGCTGTTTGCCAATGTCGAG
CTGCCGGTGCATTAG

Upstream 100 bases:

>100_bases
ACAAAGGTGATAAAAAGTTCTAGAATTATTCGTCTCACCATTAATCCAGTTATATACCGTCGCCGATACCCATAGGGTTT
ATCTGCTTTGAGGGATACAT

Downstream 100 bases:

>100_bases
AGGCAGTTTTGAGCTACAAGACAAGCTACAAGCTACAAGCTACAAGCTACAAGCTACAAGCCAGAGCCCTACGCGCTTTG
ACTTGAAGCTTGAAGCTTGA

Product: transcriptional regulator CysB

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKLQQLRYIWEVAHHDLNVSATAQSLYTSQPGISKQIRLLEDELGVEVFARSGKHLTRVTPAGERIITTAGEILRKVESI
KQIAQEFSNEKKGTLSIATTHTQARYALPSVISNFIKQYPDVALHMHQGSPMQIAEMAADGTVDFAIATEALEQFSDLVM
MPCYRWNRCVVVPQGHPLTKAPKLTLELLAEYPIVTYVFGFTGRSKLDEAFSHRGLVPKVVFTAADADVIKTYVRLNLGV
GIVARMAVDEEVDSDLVVLDASDLFESSVTKIAFRRGTFLRGFMCDFIEKFAPHLTREVMAKAIQCHNKQEMEELFANVE
LPVH

Sequences:

>Translated_324_residues
MKLQQLRYIWEVAHHDLNVSATAQSLYTSQPGISKQIRLLEDELGVEVFARSGKHLTRVTPAGERIITTAGEILRKVESI
KQIAQEFSNEKKGTLSIATTHTQARYALPSVISNFIKQYPDVALHMHQGSPMQIAEMAADGTVDFAIATEALEQFSDLVM
MPCYRWNRCVVVPQGHPLTKAPKLTLELLAEYPIVTYVFGFTGRSKLDEAFSHRGLVPKVVFTAADADVIKTYVRLNLGV
GIVARMAVDEEVDSDLVVLDASDLFESSVTKIAFRRGTFLRGFMCDFIEKFAPHLTREVMAKAIQCHNKQEMEELFANVE
LPVH
>Mature_324_residues
MKLQQLRYIWEVAHHDLNVSATAQSLYTSQPGISKQIRLLEDELGVEVFARSGKHLTRVTPAGERIITTAGEILRKVESI
KQIAQEFSNEKKGTLSIATTHTQARYALPSVISNFIKQYPDVALHMHQGSPMQIAEMAADGTVDFAIATEALEQFSDLVM
MPCYRWNRCVVVPQGHPLTKAPKLTLELLAEYPIVTYVFGFTGRSKLDEAFSHRGLVPKVVFTAADADVIKTYVRLNLGV
GIVARMAVDEEVDSDLVVLDASDLFESSVTKIAFRRGTFLRGFMCDFIEKFAPHLTREVMAKAIQCHNKQEMEELFANVE
LPVH

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=322, Percent_Identity=63.0434782608696, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1788296, Length=313, Percent_Identity=40.8945686900958, Blast_Score=267, Evalue=8e-73,
Organism=Escherichia coli, GI157672245, Length=235, Percent_Identity=29.3617021276596, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790399, Length=282, Percent_Identity=24.468085106383, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787879, Length=253, Percent_Identity=28.4584980237154, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788481, Length=260, Percent_Identity=26.5384615384615, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787601, Length=149, Percent_Identity=29.5302013422819, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788887, Length=245, Percent_Identity=26.9387755102041, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1789204, Length=262, Percent_Identity=24.8091603053435, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790262, Length=192, Percent_Identity=27.6041666666667, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693105, Length=177, Percent_Identity=24.2937853107345, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36252; Mature: 36252

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQQLRYIWEVAHHDLNVSATAQSLYTSQPGISKQIRLLEDELGVEVFARSGKHLTRVT
CCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHCCCCCEEEEC
PAGERIITTAGEILRKVESIKQIAQEFSNEKKGTLSIATTHTQARYALPSVISNFIKQYP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCC
DVALHMHQGSPMQIAEMAADGTVDFAIATEALEQFSDLVMMPCYRWNRCVVVPQGHPLTK
CEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCC
APKLTLELLAEYPIVTYVFGFTGRSKLDEAFSHRGLVPKVVFTAADADVIKTYVRLNLGV
CHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHCCCCHHHHEECCCHHHHHHHHHHHCCH
GIVARMAVDEEVDSDLVVLDASDLFESSVTKIAFRRGTFLRGFMCDFIEKFAPHLTREVM
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
AKAIQCHNKQEMEELFANVELPVH
HHHHHHCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKLQQLRYIWEVAHHDLNVSATAQSLYTSQPGISKQIRLLEDELGVEVFARSGKHLTRVT
CCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHCCCCCEEEEC
PAGERIITTAGEILRKVESIKQIAQEFSNEKKGTLSIATTHTQARYALPSVISNFIKQYP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCC
DVALHMHQGSPMQIAEMAADGTVDFAIATEALEQFSDLVMMPCYRWNRCVVVPQGHPLTK
CEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCC
APKLTLELLAEYPIVTYVFGFTGRSKLDEAFSHRGLVPKVVFTAADADVIKTYVRLNLGV
CHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHCCCCHHHHEECCCHHHHHHHHHHHCCH
GIVARMAVDEEVDSDLVVLDASDLFESSVTKIAFRRGTFLRGFMCDFIEKFAPHLTREVM
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
AKAIQCHNKQEMEELFANVELPVH
HHHHHHCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]