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The map label for this gene is cheB2

Identifier: 66044556

GI number: 66044556

Start: 1484946

End: 1485956

Strand: Direct

Name: cheB2

Synonym: Psyr_1308

Alternate gene names: 66044556

Gene position: 1484946-1485956 (Clockwise)

Preceding gene: 66044555

Following gene: 66044557

Centisome position: 24.37

GC content: 62.91

Gene sequence:

>1011_bases
ATGAAGATTGCCATCGTCAACGATATGCCCATGGCCATCGAAGCCCTGCGCCGTGCGCTGGCCTTCGAGCCCGCGCATCA
GATCATCTGGGTGGCGTCCAATGGTGCCGACGCCGTGCAGCGTTGCGTCGAGCAGACCCCGGACCTGATCCTGATGGACC
TGATCATGCCGGTGATGGATGGCGTGGAAGCCACGCGCCGGATCATGGCCGAAACACCCTGCGCCATCGTCATCGTGACC
GTCGACCACGAGCAGAACATGCGTCGCGTGTTCGAAGCGATGGGGCACGGCGCGCTGGATGTGGTGGACACGCCGGCCAT
TGGTGGTCCCAATCCAAAAGAGGCGGCGGCGCCACTGCTGCGCAAGATCCTCAATATCGACTGGCTGATCGGCCAGCGTG
TCGGCCTCGAACGGGTTGCGGCTACACCGCGCGCCGCGCCCTCGAGACGCGATCGGCTTGTGGCGATCGGCTCCTCGGCA
GGCGGGCCTGCCGCGCTGGAGATTCTGCTCAAGGCGCTTCCGGTGAGCTTTCCTGCAGCGATCGTCCTTGTGCAGCACGT
TGATCAGGTCTTCGCCGCCGGTATGGCCGACTGGTTGAGTTCCGCCTCCGGCCTGCCGGTACGGCTGGCAAGGGAAGGCG
AAACGCCACAGCCGGGTGTGGTGTTGCTGGCAGGCACCAATCATCATATCCGCTTGCTCAAGGACGGCACGCTGGCGTAC
ACGGCGGAGCCGGTGAATGAAGTTTACAGGCCCTCGATTGATGTGTTTTTCGAGAGCGTAACCCGTTATTGGAGTGGCGA
GGCGATCGGCGTTCTGCTGACCGGGATGGGGCGCGACGGTGCACAGGGACTCAAGGCCATGCGTGAACGGGGGTTTCTGA
CCATCGCTCAGGATCAGGCCAGTTCTGCTGTGTATGGCATGCCCAAGGCCGCCGCAGCCATCGATGCTGCCGTGGAAATC
CGTCCGTTGCCCGCCATAGCACCTCGCTTGATCGAGGTATTTACTCAATGA

Upstream 100 bases:

>100_bases
GCGGCCTGGACGCCGGCGCCGATTATTACCTGGCCAAGGCCAGTTTCCACGATGATGCCTTGCTCGACGCTGTCGTCGAA
TTGATAGGAGACGCGCAAGG

Downstream 100 bases:

>100_bases
CTGCTCTGCTGAACCCAGCGGCAGTCGGCGATCAGGAGAAAGATCATGAATGACCTGCAAACGGATGAGCTCAAGACACC
GGATGAAAACAGCGCAATGG

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKIAIVNDMPMAIEALRRALAFEPAHQIIWVASNGADAVQRCVEQTPDLILMDLIMPVMDGVEATRRIMAETPCAIVIVT
VDHEQNMRRVFEAMGHGALDVVDTPAIGGPNPKEAAAPLLRKILNIDWLIGQRVGLERVAATPRAAPSRRDRLVAIGSSA
GGPAALEILLKALPVSFPAAIVLVQHVDQVFAAGMADWLSSASGLPVRLAREGETPQPGVVLLAGTNHHIRLLKDGTLAY
TAEPVNEVYRPSIDVFFESVTRYWSGEAIGVLLTGMGRDGAQGLKAMRERGFLTIAQDQASSAVYGMPKAAAAIDAAVEI
RPLPAIAPRLIEVFTQ

Sequences:

>Translated_336_residues
MKIAIVNDMPMAIEALRRALAFEPAHQIIWVASNGADAVQRCVEQTPDLILMDLIMPVMDGVEATRRIMAETPCAIVIVT
VDHEQNMRRVFEAMGHGALDVVDTPAIGGPNPKEAAAPLLRKILNIDWLIGQRVGLERVAATPRAAPSRRDRLVAIGSSA
GGPAALEILLKALPVSFPAAIVLVQHVDQVFAAGMADWLSSASGLPVRLAREGETPQPGVVLLAGTNHHIRLLKDGTLAY
TAEPVNEVYRPSIDVFFESVTRYWSGEAIGVLLTGMGRDGAQGLKAMRERGFLTIAQDQASSAVYGMPKAAAAIDAAVEI
RPLPAIAPRLIEVFTQ
>Mature_336_residues
MKIAIVNDMPMAIEALRRALAFEPAHQIIWVASNGADAVQRCVEQTPDLILMDLIMPVMDGVEATRRIMAETPCAIVIVT
VDHEQNMRRVFEAMGHGALDVVDTPAIGGPNPKEAAAPLLRKILNIDWLIGQRVGLERVAATPRAAPSRRDRLVAIGSSA
GGPAALEILLKALPVSFPAAIVLVQHVDQVFAAGMADWLSSASGLPVRLAREGETPQPGVVLLAGTNHHIRLLKDGTLAY
TAEPVNEVYRPSIDVFFESVTRYWSGEAIGVLLTGMGRDGAQGLKAMRERGFLTIAQDQASSAVYGMPKAAAAIDAAVEI
RPLPAIAPRLIEVFTQ

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=304, Percent_Identity=31.25, Blast_Score=142, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB2_PSEU2 (Q4ZWW3)

Other databases:

- EMBL:   CP000075
- RefSeq:   YP_234397.1
- ProteinModelPortal:   Q4ZWW3
- STRING:   Q4ZWW3
- GeneID:   3366803
- GenomeReviews:   CP000075_GR
- KEGG:   psb:Psyr_1308
- NMPDR:   fig|205918.4.peg.1622
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   FFESVVE
- ProtClustDB:   PRK12555
- BioCyc:   PSYR205918:PSYR_1308-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 35962; Mature: 35962

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 159-159 ACT_SITE 186-186 ACT_SITE 279-279

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAIVNDMPMAIEALRRALAFEPAHQIIWVASNGADAVQRCVEQTPDLILMDLIMPVMD
CEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHC
GVEATRRIMAETPCAIVIVTVDHEQNMRRVFEAMGHGALDVVDTPAIGGPNPKEAAAPLL
CHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHH
RKILNIDWLIGQRVGLERVAATPRAAPSRRDRLVAIGSSAGGPAALEILLKALPVSFPAA
HHHHCCHHHHHCCCCHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHH
IVLVQHVDQVFAAGMADWLSSASGLPVRLAREGETPQPGVVLLAGTNHHIRLLKDGTLAY
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEECCCCEEEEEECCCEEE
TAEPVNEVYRPSIDVFFESVTRYWSGEAIGVLLTGMGRDGAQGLKAMRERGFLTIAQDQA
ECCHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCC
SSAVYGMPKAAAAIDAAVEIRPLPAIAPRLIEVFTQ
CCHHCCCCHHHHHHHHHHEECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKIAIVNDMPMAIEALRRALAFEPAHQIIWVASNGADAVQRCVEQTPDLILMDLIMPVMD
CEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHC
GVEATRRIMAETPCAIVIVTVDHEQNMRRVFEAMGHGALDVVDTPAIGGPNPKEAAAPLL
CHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHH
RKILNIDWLIGQRVGLERVAATPRAAPSRRDRLVAIGSSAGGPAALEILLKALPVSFPAA
HHHHCCHHHHHCCCCHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHH
IVLVQHVDQVFAAGMADWLSSASGLPVRLAREGETPQPGVVLLAGTNHHIRLLKDGTLAY
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEECCCCEEEEEECCCEEE
TAEPVNEVYRPSIDVFFESVTRYWSGEAIGVLLTGMGRDGAQGLKAMRERGFLTIAQDQA
ECCHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCC
SSAVYGMPKAAAAIDAAVEIRPLPAIAPRLIEVFTQ
CCHHCCCCHHHHHHHHHHEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA