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The map label for this gene is gatA [H]

Identifier: 66044421

GI number: 66044421

Start: 1323843

End: 1325663

Strand: Reverse

Name: gatA [H]

Synonym: Psyr_1170

Alternate gene names: 66044421

Gene position: 1325663-1323843 (Counterclockwise)

Preceding gene: 66044422

Following gene: 66044420

Centisome position: 21.75

GC content: 64.03

Gene sequence:

>1821_bases
ATGAACACCACCATGACCGCACTCGATATTCCTGAACTTCAGTCGGCGCTCCGCTCCGGCGAAACCACGCTGACGACTCT
GGTGCATACCCTGACTGCGCATATTGATGCGGATGACCGGGCAGAGGTCTGGATTCATCGCGTTCCCCTGGCACAGCTTG
TCGAGCGGGCAAAGGCGCTGGAGGGCATCGCCGAAGAAATGGGCGATGCGCTGTATGACCGGTTGCCGCTGTTCGGCGTG
CCCTTTGCGGTCAAGGACAATTTCGACGTGGCGCAGATGCCGACCACGGCTGCCTGCCCGACGTTCGCCTACGTACCCGA
GGACAATGCTCACGTTGTGCAGCTGCTGCTCGACAGCGGCGCAATCCTGATGGGCAAGACCAATCTCGATCAATTCGCCA
CAGGCCTGGTGGGCGTCCGCTCGCCTTACGGCGCGGTACGCAATGCGCATGATCCGGCCTACGTCAGTGGCGGTTCGAGT
TCCGGTTCGGCAGTGGCGGTGGCGCGTGGCTACGTCAGTTTTGCACTGGGCACGGACACCGCAGGTTCAGGCCGCGTGCC
AGCCGGCTTCAACGGCATCGTCGGGCTCAAGCCCAGCCTTGGCCTGTTCAGCAGCCGCGGGGTCGTGCCTGCCTGCCGCA
CGCTGGACTGCCCATCCATCTTTGCCAACGATGTGATGCAAGCCTGGCAGGTGGCGCAGGTCATTGCAGCTTACGACCCG
CTGGATGCGTCCAGTGTGGCCGTGCAGGCATTGCCGGTGCAGCGCAGGGTTCGGCGTGTCGCCGTCGCGCGGCACTGCGA
GTTCTTCGGCGATGAGCAGGCACAGGCGGCTTACCTCAAAACTCTGGAAGCCCTGCAAAACGACCCGCTGGTGACGGTCA
GCGCTATCGAGTTCGACGTGTTCGCCGAGGCAGCTGCCCTGCTGTATCAAGGCCCGTGGGTGGCGGAACGCCGCGCTGCC
ATCGGCGAATTCTTCGCCAGCAACGCGGCCGACATTCATCCCGTGGTGCGCGGCATTGTGCAGAGCGCTGATTCATTCGA
CGCCGTGGACACGTTCAACGCGCGCTATCGCCTGGCAGAGCTGACCCGCGCTGCGCAGCAACTGCTGACCGACATCGATG
TGCTGGTGGTGCCAACCGCGCCGTGCATGCCGACCATCGAAGCGGTGCTCGGCAACCCGGTCGCGCTCAACTCACAGCTC
GGCTATTACACCAACTTCGTCAACCTGATGAACATGTGCGCCATCGCCATCCCGACAGATCGTCGCGCGGACGGGCTGCC
TGCGGGTATCACGCTGATTGGCCCGGCCGGTGCCGATCAACGCCTGGCAGAGATCGCCGCCGCCTGGCAGTCGCTGTTCG
GCCAGGCTGACCAGCGTGACGCCGTGGCAATGGCTCCCCTGCCCTATAACAGCCCGACCGTACAGGTTGCGGTCGTCGGC
GCGCATCTGGTCGGCCAACCGCTCAACTGGCAACTGCTTGAAGGTGGCGCTCGCTTGCTGCGCAGCACAACCACCAGCGC
GGATTACCGCCTGTATGCGCTGGCAGAAACGTCGCCGCCGAAGCCGGGACTGGTGCGCGTCGCTGAACAGGGCGCGAGCA
TCGAGATAGAAGTCTGGGAAATGCCGCTGAACCAGTTCGGCGCTTTCGTCGCAGCCATTCCCGCGCCACTGGGCATCGGC
TCGCTGCAATTGGCCGACGGCCAGTGGGTCAAGGGTTTCATCTGTGAGCCTGGCGGCCTTGAAGGCGCGCTGGACATCAC
TGACTACAAAGGCTGGCGCGCCTACCGCGCCGCGCAAACACCGTCTTCCATCGCTCATTGA

Upstream 100 bases:

>100_bases
CGCAACTCACCGCCAATAGCCAGTAATCCCGCCGTAAATCGCCCTCCGGCCGACGTTGGCCCATAACGTGCAAACGCGTC
AGTTCATCTTGTATACATGA

Downstream 100 bases:

>100_bases
TCAGGGGTAGCAGCATGTCGAACGTTGATCGTCGCAGTTTCATCAAGTTGGCGGGCATTATCGGCCTGAGCGCAGCGCTG
CCTTTCGGTCGCGCCTATTC

Product: allophanate hydrolase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 606; Mature: 606

Protein sequence:

>606_residues
MNTTMTALDIPELQSALRSGETTLTTLVHTLTAHIDADDRAEVWIHRVPLAQLVERAKALEGIAEEMGDALYDRLPLFGV
PFAVKDNFDVAQMPTTAACPTFAYVPEDNAHVVQLLLDSGAILMGKTNLDQFATGLVGVRSPYGAVRNAHDPAYVSGGSS
SGSAVAVARGYVSFALGTDTAGSGRVPAGFNGIVGLKPSLGLFSSRGVVPACRTLDCPSIFANDVMQAWQVAQVIAAYDP
LDASSVAVQALPVQRRVRRVAVARHCEFFGDEQAQAAYLKTLEALQNDPLVTVSAIEFDVFAEAAALLYQGPWVAERRAA
IGEFFASNAADIHPVVRGIVQSADSFDAVDTFNARYRLAELTRAAQQLLTDIDVLVVPTAPCMPTIEAVLGNPVALNSQL
GYYTNFVNLMNMCAIAIPTDRRADGLPAGITLIGPAGADQRLAEIAAAWQSLFGQADQRDAVAMAPLPYNSPTVQVAVVG
AHLVGQPLNWQLLEGGARLLRSTTTSADYRLYALAETSPPKPGLVRVAEQGASIEIEVWEMPLNQFGAFVAAIPAPLGIG
SLQLADGQWVKGFICEPGGLEGALDITDYKGWRAYRAAQTPSSIAH

Sequences:

>Translated_606_residues
MNTTMTALDIPELQSALRSGETTLTTLVHTLTAHIDADDRAEVWIHRVPLAQLVERAKALEGIAEEMGDALYDRLPLFGV
PFAVKDNFDVAQMPTTAACPTFAYVPEDNAHVVQLLLDSGAILMGKTNLDQFATGLVGVRSPYGAVRNAHDPAYVSGGSS
SGSAVAVARGYVSFALGTDTAGSGRVPAGFNGIVGLKPSLGLFSSRGVVPACRTLDCPSIFANDVMQAWQVAQVIAAYDP
LDASSVAVQALPVQRRVRRVAVARHCEFFGDEQAQAAYLKTLEALQNDPLVTVSAIEFDVFAEAAALLYQGPWVAERRAA
IGEFFASNAADIHPVVRGIVQSADSFDAVDTFNARYRLAELTRAAQQLLTDIDVLVVPTAPCMPTIEAVLGNPVALNSQL
GYYTNFVNLMNMCAIAIPTDRRADGLPAGITLIGPAGADQRLAEIAAAWQSLFGQADQRDAVAMAPLPYNSPTVQVAVVG
AHLVGQPLNWQLLEGGARLLRSTTTSADYRLYALAETSPPKPGLVRVAEQGASIEIEVWEMPLNQFGAFVAAIPAPLGIG
SLQLADGQWVKGFICEPGGLEGALDITDYKGWRAYRAAQTPSSIAH
>Mature_606_residues
MNTTMTALDIPELQSALRSGETTLTTLVHTLTAHIDADDRAEVWIHRVPLAQLVERAKALEGIAEEMGDALYDRLPLFGV
PFAVKDNFDVAQMPTTAACPTFAYVPEDNAHVVQLLLDSGAILMGKTNLDQFATGLVGVRSPYGAVRNAHDPAYVSGGSS
SGSAVAVARGYVSFALGTDTAGSGRVPAGFNGIVGLKPSLGLFSSRGVVPACRTLDCPSIFANDVMQAWQVAQVIAAYDP
LDASSVAVQALPVQRRVRRVAVARHCEFFGDEQAQAAYLKTLEALQNDPLVTVSAIEFDVFAEAAALLYQGPWVAERRAA
IGEFFASNAADIHPVVRGIVQSADSFDAVDTFNARYRLAELTRAAQQLLTDIDVLVVPTAPCMPTIEAVLGNPVALNSQL
GYYTNFVNLMNMCAIAIPTDRRADGLPAGITLIGPAGADQRLAEIAAAWQSLFGQADQRDAVAMAPLPYNSPTVQVAVVG
AHLVGQPLNWQLLEGGARLLRSTTTSADYRLYALAETSPPKPGLVRVAEQGASIEIEVWEMPLNQFGAFVAAIPAPLGIG
SLQLADGQWVKGFICEPGGLEGALDITDYKGWRAYRAAQTPSSIAH

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=504, Percent_Identity=27.9761904761905, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI195972892, Length=153, Percent_Identity=33.9869281045752, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=421, Percent_Identity=28.2660332541568, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17537465, Length=479, Percent_Identity=25.4697286012526, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17556264, Length=209, Percent_Identity=31.5789473684211, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=212, Percent_Identity=27.3584905660377, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=200, Percent_Identity=30.5, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=205, Percent_Identity=31.7073170731707, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=205, Percent_Identity=31.7073170731707, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=590, Percent_Identity=43.3898305084746, Blast_Score=425, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6323950, Length=358, Percent_Identity=29.608938547486, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648113, Length=177, Percent_Identity=42.3728813559322, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356731, Length=484, Percent_Identity=23.5537190082645, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652985, Length=438, Percent_Identity=24.6575342465753, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19922090, Length=438, Percent_Identity=24.6575342465753, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24652981, Length=438, Percent_Identity=24.6575342465753, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24652983, Length=438, Percent_Identity=24.6575342465753, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24644968, Length=499, Percent_Identity=24.248496993988, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 64340; Mature: 64340

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTMTALDIPELQSALRSGETTLTTLVHTLTAHIDADDRAEVWIHRVPLAQLVERAKAL
CCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHEEEECCHHHHHHHHHHH
EGIAEEMGDALYDRLPLFGVPFAVKDNFDVAQMPTTAACPTFAYVPEDNAHVVQLLLDSG
HHHHHHHHHHHHHCCCCCCCCEEEECCCCHHCCCCCCCCCEEEECCCCCCCEEEEEHHCC
AILMGKTNLDQFATGLVGVRSPYGAVRNAHDPAYVSGGSSSGSAVAVARGYVSFALGTDT
EEEEECCCHHHHHHHHHHCCCCHHHHCCCCCCCEEECCCCCCCEEEEEECEEEEEECCCC
AGSGRVPAGFNGIVGLKPSLGLFSSRGVVPACRTLDCPSIFANDVMQAWQVAQVIAAYDP
CCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
LDASSVAVQALPVQRRVRRVAVARHCEFFGDEQAQAAYLKTLEALQNDPLVTVSAIEFDV
CCCCHHHEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEHHH
FAEAAALLYQGPWVAERRAAIGEFFASNAADIHPVVRGIVQSADSFDAVDTFNARYRLAE
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHCCHHHHHHH
LTRAAQQLLTDIDVLVVPTAPCMPTIEAVLGNPVALNSQLGYYTNFVNLMNMCAIAIPTD
HHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHEEECCCC
RRADGLPAGITLIGPAGADQRLAEIAAAWQSLFGQADQRDAVAMAPLPYNSPTVQVAVVG
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCEEEEEEEE
AHLVGQPLNWQLLEGGARLLRSTTTSADYRLYALAETSPPKPGLVRVAEQGASIEIEVWE
HHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEECCCCEEEEEEEE
MPLNQFGAFVAAIPAPLGIGSLQLADGQWVKGFICEPGGLEGALDITDYKGWRAYRAAQT
CCHHHHHHHHHHHCCCCCCCCEEECCCCEEEEEEECCCCCCCEEEECCCCCCHHHCCCCC
PSSIAH
CHHCCC
>Mature Secondary Structure
MNTTMTALDIPELQSALRSGETTLTTLVHTLTAHIDADDRAEVWIHRVPLAQLVERAKAL
CCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHEEEECCHHHHHHHHHHH
EGIAEEMGDALYDRLPLFGVPFAVKDNFDVAQMPTTAACPTFAYVPEDNAHVVQLLLDSG
HHHHHHHHHHHHHCCCCCCCCEEEECCCCHHCCCCCCCCCEEEECCCCCCCEEEEEHHCC
AILMGKTNLDQFATGLVGVRSPYGAVRNAHDPAYVSGGSSSGSAVAVARGYVSFALGTDT
EEEEECCCHHHHHHHHHHCCCCHHHHCCCCCCCEEECCCCCCCEEEEEECEEEEEECCCC
AGSGRVPAGFNGIVGLKPSLGLFSSRGVVPACRTLDCPSIFANDVMQAWQVAQVIAAYDP
CCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
LDASSVAVQALPVQRRVRRVAVARHCEFFGDEQAQAAYLKTLEALQNDPLVTVSAIEFDV
CCCCHHHEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEHHH
FAEAAALLYQGPWVAERRAAIGEFFASNAADIHPVVRGIVQSADSFDAVDTFNARYRLAE
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHCCHHHHHHH
LTRAAQQLLTDIDVLVVPTAPCMPTIEAVLGNPVALNSQLGYYTNFVNLMNMCAIAIPTD
HHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHEEECCCC
RRADGLPAGITLIGPAGADQRLAEIAAAWQSLFGQADQRDAVAMAPLPYNSPTVQVAVVG
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCEEEEEEEE
AHLVGQPLNWQLLEGGARLLRSTTTSADYRLYALAETSPPKPGLVRVAEQGASIEIEVWE
HHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEECCCCEEEEEEEE
MPLNQFGAFVAAIPAPLGIGSLQLADGQWVKGFICEPGGLEGALDITDYKGWRAYRAAQT
CCHHHHHHHHHHHCCCCCCCCEEECCCCEEEEEEECCCCCCCEEEECCCCCCHHHCCCCC
PSSIAH
CHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA