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The map label for this gene is amiF [H]

Identifier: 66044417

GI number: 66044417

Start: 1319643

End: 1320659

Strand: Reverse

Name: amiF [H]

Synonym: Psyr_1166

Alternate gene names: 66044417

Gene position: 1320659-1319643 (Counterclockwise)

Preceding gene: 66044418

Following gene: 66044416

Centisome position: 21.67

GC content: 63.23

Gene sequence:

>1017_bases
ATGGCCAGTGGTCTTGGTGGTTTGAACAAGTCGCCCAATGGCGTGGTGATCGGCCTGGCGCAACTGGCGCTGCCCGATCC
GCATACGCGCGAGGCGTTGTGGATGCAGACGCAGAAAGTGGTCGGCATGGTTGCCAAGGCTCGGCGCAGCAATCCGGGCA
TGGATCTGATCGTATTCCCCGAATACTCGCTGCACGGCCTGTCGATGAGCACGGCGCCGGAGATCATGTGCAGCCTCGAC
GGCCCGGAAGTCATGGCACTGCGCGAGGCGTGCAAGACGCACCGGATATGGGGCTGCTTCTCGATCATGGAAGCCAACCC
GCACGGCAACCCGTTCAACAGTGGCCTGATCGTCGATGACCTTGGAGAGATCCGCCTGTACTACCGCAAGCTGCACCCGT
GGGTGCCGGTCGAACCGTGGGAGCCGGGCGATCTGGGTATTCCGGTCTGCGACGGACCGCGCGGTAGCAAGCTGGCGCTG
ATCATCTGCCATGACGGCATGTTCCCGGAAATGGCCCGCGAGTGCGCCTACAAGGGCGCCGACATCATGCTGCGCACCGC
GGGTTACACCGCACCGATCCGCCACTCGTGGAAAATCACCAATCAGAGCAACGCCTTCACCAACCTGATGCAGACCGCAA
GCGTCTGCATGTGCGGCTCCGACGGCACGTTCGACTCCATGGGCGAGGCGATGTTTGTCGACTTCGACGGCACGATCATG
GCCGAAGGGGGCGGCCGGGCCGACGAAATCGTCTGCTGCGAACTGCGCCCCGACCTGGTCCGCGAAGCCCGTGTGCACTG
GGGCGTGGAAAACAACATCTATCAATTCGGGCATCGCGGCTATGTGGCGGTCAAGGGCGGCGCACGCGACTGCCCTTACA
CCTACATGCACGATCTGAGCGCAGGCCGATACCGTCTGCCGTGGGAAGACGATGTCGTGCATACCGATGGCAGCTCGTGC
GGTTTTGCGGCCCCCGAGCGCGACTTCAAACCCACCCCCGGCAGCTGGAAGGAGTAA

Upstream 100 bases:

>100_bases
GGTGATGTACGCCACCTGTCGCCGTGGTCGCACTTTGAACGGCGCGCCGGGCGAACTCAGCCTGACGCGATGAACTGATC
TTTTTTCGAGGAGACATTGC

Downstream 100 bases:

>100_bases
CGCCATGAGCGAACGTCATATTGCATCGGCGCCCTACCCCTGGCCCTGGAACGGGCAATTGCATGCGCACAACACGGCCT
TGATCGTGATCGACATGCAG

Product: formamidase

Products: NA

Alternate protein names: Formamide amidohydrolase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MASGLGGLNKSPNGVVIGLAQLALPDPHTREALWMQTQKVVGMVAKARRSNPGMDLIVFPEYSLHGLSMSTAPEIMCSLD
GPEVMALREACKTHRIWGCFSIMEANPHGNPFNSGLIVDDLGEIRLYYRKLHPWVPVEPWEPGDLGIPVCDGPRGSKLAL
IICHDGMFPEMARECAYKGADIMLRTAGYTAPIRHSWKITNQSNAFTNLMQTASVCMCGSDGTFDSMGEAMFVDFDGTIM
AEGGGRADEIVCCELRPDLVREARVHWGVENNIYQFGHRGYVAVKGGARDCPYTYMHDLSAGRYRLPWEDDVVHTDGSSC
GFAAPERDFKPTPGSWKE

Sequences:

>Translated_338_residues
MASGLGGLNKSPNGVVIGLAQLALPDPHTREALWMQTQKVVGMVAKARRSNPGMDLIVFPEYSLHGLSMSTAPEIMCSLD
GPEVMALREACKTHRIWGCFSIMEANPHGNPFNSGLIVDDLGEIRLYYRKLHPWVPVEPWEPGDLGIPVCDGPRGSKLAL
IICHDGMFPEMARECAYKGADIMLRTAGYTAPIRHSWKITNQSNAFTNLMQTASVCMCGSDGTFDSMGEAMFVDFDGTIM
AEGGGRADEIVCCELRPDLVREARVHWGVENNIYQFGHRGYVAVKGGARDCPYTYMHDLSAGRYRLPWEDDVVHTDGSSC
GFAAPERDFKPTPGSWKE
>Mature_337_residues
ASGLGGLNKSPNGVVIGLAQLALPDPHTREALWMQTQKVVGMVAKARRSNPGMDLIVFPEYSLHGLSMSTAPEIMCSLDG
PEVMALREACKTHRIWGCFSIMEANPHGNPFNSGLIVDDLGEIRLYYRKLHPWVPVEPWEPGDLGIPVCDGPRGSKLALI
ICHDGMFPEMARECAYKGADIMLRTAGYTAPIRHSWKITNQSNAFTNLMQTASVCMCGSDGTFDSMGEAMFVDFDGTIMA
EGGGRADEIVCCELRPDLVREARVHWGVENNIYQFGHRGYVAVKGGARDCPYTYMHDLSAGRYRLPWEDDVVHTDGSSCG
FAAPERDFKPTPGSWKE

Specific function: Is an aliphatic amidase with a restricted substrate specificity, as it only hydrolyzes formamide [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022843
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.49 [H]

Molecular weight: Translated: 37177; Mature: 37046

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
8.6 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGLGGLNKSPNGVVIGLAQLALPDPHTREALWMQTQKVVGMVAKARRSNPGMDLIVFP
CCCCCCCCCCCCCEEEEEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
EYSLHGLSMSTAPEIMCSLDGPEVMALREACKTHRIWGCFSIMEANPHGNPFNSGLIVDD
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCEEEEC
LGEIRLYYRKLHPWVPVEPWEPGDLGIPVCDGPRGSKLALIICHDGMFPEMARECAYKGA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCC
DIMLRTAGYTAPIRHSWKITNQSNAFTNLMQTASVCMCGSDGTFDSMGEAMFVDFDGTIM
CEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHEEEECCCCCHHHCCCEEEEEECCEEE
AEGGGRADEIVCCELRPDLVREARVHWGVENNIYQFGHRGYVAVKGGARDCPYTYMHDLS
ECCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCEEECCCCEEEECCCCCCCCHHHHHHCC
AGRYRLPWEDDVVHTDGSSCGFAAPERDFKPTPGSWKE
CCCEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASGLGGLNKSPNGVVIGLAQLALPDPHTREALWMQTQKVVGMVAKARRSNPGMDLIVFP
CCCCCCCCCCCCEEEEEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
EYSLHGLSMSTAPEIMCSLDGPEVMALREACKTHRIWGCFSIMEANPHGNPFNSGLIVDD
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCEEEEC
LGEIRLYYRKLHPWVPVEPWEPGDLGIPVCDGPRGSKLALIICHDGMFPEMARECAYKGA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCC
DIMLRTAGYTAPIRHSWKITNQSNAFTNLMQTASVCMCGSDGTFDSMGEAMFVDFDGTIM
CEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHEEEECCCCCHHHCCCEEEEEECCEEE
AEGGGRADEIVCCELRPDLVREARVHWGVENNIYQFGHRGYVAVKGGARDCPYTYMHDLS
ECCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCEEECCCCEEEECCCCCCCCHHHHHHCC
AGRYRLPWEDDVVHTDGSSCGFAAPERDFKPTPGSWKE
CCCEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]