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The map label for this gene is dadA [C]

Identifier: 66043986

GI number: 66043986

Start: 824797

End: 825900

Strand: Direct

Name: dadA [C]

Synonym: Psyr_0721

Alternate gene names: 66043986

Gene position: 824797-825900 (Clockwise)

Preceding gene: 66043985

Following gene: 66043991

Centisome position: 13.54

GC content: 62.05

Gene sequence:

>1104_bases
ATGTCCAAGCAAAAAATAGTCATTGTCGGCGGTGGCGTCATAGGTCTGTTGACCGCCTTCAATCTGGCGTCCGAGCAGGT
CAGCGTCGTGCTGCTGGATCGTTCGGGCGTTGGTCAGGAGTCTTCCTGGGCGGGCGGCGGGATTGTCTCGCCGCTTTACC
CGTGGCGCTACAGCCCTGCGGTCACCGCGCTGGCGCACTGGTCGCAGGATTTCTACCCGCGCCTCGCCGAGCGTTTGCTC
GCACAGACCGGTATCGATCCTGAAGTGCACAAGACTGGCCTGTACTGGCTCGATCTTGATGACGAGCAGTCTGCGCTTGA
ATGGGCTGTCCGCGAAAAACGTCCGTTGAACCGCGTGGATATCTCGGCGGTAAATGATGCCGTGCCGGTGCTGGGTGAGG
GCTATTCGCGGGCGATCTACATGGCCGATGTGGCCAACGTACGCAATCCCCGCCTGGTCAAATCGCTCAAGGCCGCCTTG
CTGGCAATGCCCAATGTCGAGATTCGCGAGCAGTGTGAAGTCAGCGGCTTTACCCGTGAAGGCAGCAGGATTTCGGGTGT
GCAGACACCTGCAGGGGATATCACCGGTGATCGGGTGATTCTGGCGGCGGGTGCGTGGAGCGGCGAGTTGCTCAAGACTC
TGGGGCTGGAGCTGCCGGTCGAGCCGGTAAAGGGCCAGATGATTCTCTACAAGTGCGCATCGGACTTTCTGTCGAGCATG
GTGCTGGCGAAAGGGCGCTACGCGATCCCGCGCCGTGACGGGCATATCCTGATCGGCAGCACCCTGGAGCACGAAGGTTT
CGACAAAACCACCACGCAGGCCGCATTGGAAAGTTTGAAGGCATCGGCCGTCGAATTGCTTCCGCCATTGGCCCATGCCG
AGCCCGTCTCCCAGTGGGCAGGCCTGCGCCCTGGCTCGCCTGAGGGCATCCCGTTCATTGGACCGCTGGACGGTTTTGAC
GGCCTGTGGCTCAACTGCGGGCATTACCGCAACGGCCTGGTGCTGGCTCCGGCGTCCTGCCAGTTGATCACTGACCTGCT
GCTGGACCGCGAGCCGATCATCGATCCGGCACCCTACGCTCCTGCGGGGCGCCTCGCAGTCTAG

Upstream 100 bases:

>100_bases
ATTACTAACACCGGAGCGCGTACCAACACGGGCGCAACCAGCGGCGTCACTGTCAAAGACTGCTGGGGCCGCTAAACCGT
TTCCTTGTCACGAGACCAAG

Downstream 100 bases:

>100_bases
TCATGCTCTCGGGCACGAGGGTGTTCTCACGCGCACCTATCGTCCGCACGCTCCAGCGTGGGAACGCCTTTCGTGACACT
CTGCGTCACATAGTTGCTGC

Product: FAD dependent oxidoreductase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSKQKIVIVGGGVIGLLTAFNLASEQVSVVLLDRSGVGQESSWAGGGIVSPLYPWRYSPAVTALAHWSQDFYPRLAERLL
AQTGIDPEVHKTGLYWLDLDDEQSALEWAVREKRPLNRVDISAVNDAVPVLGEGYSRAIYMADVANVRNPRLVKSLKAAL
LAMPNVEIREQCEVSGFTREGSRISGVQTPAGDITGDRVILAAGAWSGELLKTLGLELPVEPVKGQMILYKCASDFLSSM
VLAKGRYAIPRRDGHILIGSTLEHEGFDKTTTQAALESLKASAVELLPPLAHAEPVSQWAGLRPGSPEGIPFIGPLDGFD
GLWLNCGHYRNGLVLAPASCQLITDLLLDREPIIDPAPYAPAGRLAV

Sequences:

>Translated_367_residues
MSKQKIVIVGGGVIGLLTAFNLASEQVSVVLLDRSGVGQESSWAGGGIVSPLYPWRYSPAVTALAHWSQDFYPRLAERLL
AQTGIDPEVHKTGLYWLDLDDEQSALEWAVREKRPLNRVDISAVNDAVPVLGEGYSRAIYMADVANVRNPRLVKSLKAAL
LAMPNVEIREQCEVSGFTREGSRISGVQTPAGDITGDRVILAAGAWSGELLKTLGLELPVEPVKGQMILYKCASDFLSSM
VLAKGRYAIPRRDGHILIGSTLEHEGFDKTTTQAALESLKASAVELLPPLAHAEPVSQWAGLRPGSPEGIPFIGPLDGFD
GLWLNCGHYRNGLVLAPASCQLITDLLLDREPIIDPAPYAPAGRLAV
>Mature_366_residues
SKQKIVIVGGGVIGLLTAFNLASEQVSVVLLDRSGVGQESSWAGGGIVSPLYPWRYSPAVTALAHWSQDFYPRLAERLLA
QTGIDPEVHKTGLYWLDLDDEQSALEWAVREKRPLNRVDISAVNDAVPVLGEGYSRAIYMADVANVRNPRLVKSLKAALL
AMPNVEIREQCEVSGFTREGSRISGVQTPAGDITGDRVILAAGAWSGELLKTLGLELPVEPVKGQMILYKCASDFLSSMV
LAKGRYAIPRRDGHILIGSTLEHEGFDKTTTQAALESLKASAVELLPPLAHAEPVSQWAGLRPGSPEGIPFIGPLDGFDG
LWLNCGHYRNGLVLAPASCQLITDLLLDREPIIDPAPYAPAGRLAV

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=411, Percent_Identity=27.7372262773723, Blast_Score=112, Evalue=5e-26,
Organism=Drosophila melanogaster, GI20130091, Length=363, Percent_Identity=22.8650137741047, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 39518; Mature: 39387

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQKIVIVGGGVIGLLTAFNLASEQVSVVLLDRSGVGQESSWAGGGIVSPLYPWRYSPA
CCCCEEEEECCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHH
VTALAHWSQDFYPRLAERLLAQTGIDPEVHKTGLYWLDLDDEQSALEWAVREKRPLNRVD
HHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHCEEEEEECCCCHHHHHHHHHHCCCCCCCC
ISAVNDAVPVLGEGYSRAIYMADVANVRNPRLVKSLKAALLAMPNVEIREQCEVSGFTRE
HHHHCCCCCCCCCCCCCEEEEEEHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCC
GSRISGVQTPAGDITGDRVILAAGAWSGELLKTLGLELPVEPVKGQMILYKCASDFLSSM
CCEECCCCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHH
VLAKGRYAIPRRDGHILIGSTLEHEGFDKTTTQAALESLKASAVELLPPLAHAEPVSQWA
HHHCCCEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHC
GLRPGSPEGIPFIGPLDGFDGLWLNCGHYRNGLVLAPASCQLITDLLLDREPIIDPAPYA
CCCCCCCCCCCEECCCCCCCCCEEECCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCC
PAGRLAV
CCCCCCC
>Mature Secondary Structure 
SKQKIVIVGGGVIGLLTAFNLASEQVSVVLLDRSGVGQESSWAGGGIVSPLYPWRYSPA
CCCEEEEECCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHH
VTALAHWSQDFYPRLAERLLAQTGIDPEVHKTGLYWLDLDDEQSALEWAVREKRPLNRVD
HHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHCEEEEEECCCCHHHHHHHHHHCCCCCCCC
ISAVNDAVPVLGEGYSRAIYMADVANVRNPRLVKSLKAALLAMPNVEIREQCEVSGFTRE
HHHHCCCCCCCCCCCCCEEEEEEHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCC
GSRISGVQTPAGDITGDRVILAAGAWSGELLKTLGLELPVEPVKGQMILYKCASDFLSSM
CCEECCCCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHH
VLAKGRYAIPRRDGHILIGSTLEHEGFDKTTTQAALESLKASAVELLPPLAHAEPVSQWA
HHHCCCEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHC
GLRPGSPEGIPFIGPLDGFDGLWLNCGHYRNGLVLAPASCQLITDLLLDREPIIDPAPYA
CCCCCCCCCCCEECCCCCCCCCEEECCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCC
PAGRLAV
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 11 {D-methionine}} 12 {D-alpha-aminobutyrate}} 6 {D-phenylalanine}} 14 {D-glutamine}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 19 {D-serine}} 0.15 {cytochrome} 29 {D-alanine}} 6.4

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8682785; 10984043; 8097014 [H]