Click here to switch to the map view.

The map label for this gene is proB [H]

Identifier: 66043969

GI number: 66043969

Start: 806935

End: 808053

Strand: Direct

Name: proB [H]

Synonym: Psyr_0704

Alternate gene names: 66043969

Gene position: 806935-808053 (Clockwise)

Preceding gene: 66043968

Following gene: 66043970

Centisome position: 13.24

GC content: 64.16

Gene sequence:

>1119_bases
ATGCGCAGCAAAGTGACGGGCGCCCAGCGCTGGGTTGTGAAGATTGGCAGTGCCTTGCTGACGGCGGATGGCAAGGGTCT
GGATCGCAACGCCATGGGTGTTTGGGTGGAGCAGATGGTGGCGCTGCACGAGGCGGGTGTCGAGCTGGTACTGGTGTCTT
CCGGGGCGGTGGCTGCCGGCATGAGCCGTCTTGGCTGGACGGCCCGACCCAGCGCCATGCATGAGCTCCAGGCTGCTGCG
GCGATTGGTCAGATGGGCTTGGTGCAGGCGTGGGAGTCGAGCTTCGCCGAGCATGGCCGACATACCGCGCAGATCCTTCT
GACTCACGATGACCTGTCGGACCGCAAGCGCTACCTGAATGCCCGTAGCACCCTGCGCACGCTGGTCGAGCTTGGGGTTG
TGCCGGTCATCAATGAAAACGACACAGTGGTCACCGACGAAATCCGTTTTGGCGATAACGACACGCTGGCGGCGTTGGTT
GCCAATCTGGTCGAGGCTGATCTGCTGGTCATCCTGACCGACCGTGACGGCATGTTCGACGCTGATCCGCGCAACAACCC
GGAGGCGCAGCTGATTTACGAAGCGCGTGCCGATGATCCGGCGCTGGATGCAGTGGCCGGTGGTACCGGCGGTGCGCTGG
GTCGCGGCGGCATGCAGACCAAGTTGCGTGCAGCGCGACTGGCGGCGCGTTCCGGTGCGCATACCGTCATCGTCGGCGGG
CGCATCGAGCGCGTTCTGGCGCGGCTCAAGGGAGGGGAGCGGTTGGGTACGCTGCTGTCCCCGGAGCGCGGCATGCTCGC
AGCACGCAAGCAGTGGCTGGCAGGTCATCTGCAGACCCGCGGTACGCTGGTGCTGGATGATGGCGCTGTTGCGGCGCTGG
CCAGGGATCAGAAAAGCCTGCTGCCGGTGGGCGTCAAACTGGTCCAGGGCAGCTTCCGGCGCGGCGAGATGGTGGTATGC
GTCGCCCAGGACGGTCGCGAGATCGCTCGTGGGCTGAGTAACTACAGTGCCATCGAAGCCCAGAAAATCATTGGTCACTC
TTCCGAATCCATCGTGCGCGAGCTGGGCTATATGGCCGAGCCCGAGCTGATTCATCGGGATAACCTGATCCTCGTCTGA

Upstream 100 bases:

>100_bases
TCCGTGACTGATCGCTTGAAGTAAACTTGAACGCCGCTACGATTGTTAGCGGCGTTTTTGTATCCCGAATTTGAAAATCT
CTGGCTAGGGTTGGAAGAAG

Downstream 100 bases:

>100_bases
CTCAAGGCTGCAAAAGGAATCGTTGATGCGCTTCATGAAAGGTTTATTGGCGGCCGCGTTGCTGGTGCCGGTGCTGGTCT
CGGCCGAAGAGATCGGTCAG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRSKVTGAQRWVVKIGSALLTADGKGLDRNAMGVWVEQMVALHEAGVELVLVSSGAVAAGMSRLGWTARPSAMHELQAAA
AIGQMGLVQAWESSFAEHGRHTAQILLTHDDLSDRKRYLNARSTLRTLVELGVVPVINENDTVVTDEIRFGDNDTLAALV
ANLVEADLLVILTDRDGMFDADPRNNPEAQLIYEARADDPALDAVAGGTGGALGRGGMQTKLRAARLAARSGAHTVIVGG
RIERVLARLKGGERLGTLLSPERGMLAARKQWLAGHLQTRGTLVLDDGAVAALARDQKSLLPVGVKLVQGSFRRGEMVVC
VAQDGREIARGLSNYSAIEAQKIIGHSSESIVRELGYMAEPELIHRDNLILV

Sequences:

>Translated_372_residues
MRSKVTGAQRWVVKIGSALLTADGKGLDRNAMGVWVEQMVALHEAGVELVLVSSGAVAAGMSRLGWTARPSAMHELQAAA
AIGQMGLVQAWESSFAEHGRHTAQILLTHDDLSDRKRYLNARSTLRTLVELGVVPVINENDTVVTDEIRFGDNDTLAALV
ANLVEADLLVILTDRDGMFDADPRNNPEAQLIYEARADDPALDAVAGGTGGALGRGGMQTKLRAARLAARSGAHTVIVGG
RIERVLARLKGGERLGTLLSPERGMLAARKQWLAGHLQTRGTLVLDDGAVAALARDQKSLLPVGVKLVQGSFRRGEMVVC
VAQDGREIARGLSNYSAIEAQKIIGHSSESIVRELGYMAEPELIHRDNLILV
>Mature_372_residues
MRSKVTGAQRWVVKIGSALLTADGKGLDRNAMGVWVEQMVALHEAGVELVLVSSGAVAAGMSRLGWTARPSAMHELQAAA
AIGQMGLVQAWESSFAEHGRHTAQILLTHDDLSDRKRYLNARSTLRTLVELGVVPVINENDTVVTDEIRFGDNDTLAALV
ANLVEADLLVILTDRDGMFDADPRNNPEAQLIYEARADDPALDAVAGGTGGALGRGGMQTKLRAARLAARSGAHTVIVGG
RIERVLARLKGGERLGTLLSPERGMLAARKQWLAGHLQTRGTLVLDDGAVAALARDQKSLLPVGVKLVQGSFRRGEMVVC
VAQDGREIARGLSNYSAIEAQKIIGHSSESIVRELGYMAEPELIHRDNLILV

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=293, Percent_Identity=34.8122866894198, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI21361368, Length=295, Percent_Identity=34.5762711864407, Blast_Score=136, Evalue=3e-32,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=43.9890710382514, Blast_Score=294, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI17569969, Length=279, Percent_Identity=33.3333333333333, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17569967, Length=279, Percent_Identity=33.3333333333333, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320506, Length=411, Percent_Identity=36.2530413625304, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6321822, Length=418, Percent_Identity=31.8181818181818, Blast_Score=202, Evalue=5e-53,
Organism=Drosophila melanogaster, GI21357643, Length=291, Percent_Identity=34.3642611683849, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39803; Mature: 39803

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSKVTGAQRWVVKIGSALLTADGKGLDRNAMGVWVEQMVALHEAGVELVLVSSGAVAAG
CCCCCCHHHHHHHHHCCHHEECCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCHHHH
MSRLGWTARPSAMHELQAAAAIGQMGLVQAWESSFAEHGRHTAQILLTHDDLSDRKRYLN
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHH
ARSTLRTLVELGVVPVINENDTVVTDEIRFGDNDTLAALVANLVEADLLVILTDRDGMFD
HHHHHHHHHHHCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCC
ADPRNNPEAQLIYEARADDPALDAVAGGTGGALGRGGMQTKLRAARLAARSGAHTVIVGG
CCCCCCCCEEEEEEECCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECC
RIERVLARLKGGERLGTLLSPERGMLAARKQWLAGHLQTRGTLVLDDGAVAALARDQKSL
HHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEHHHCCHHH
LPVGVKLVQGSFRRGEMVVCVAQDGREIARGLSNYSAIEAQKIIGHSSESIVRELGYMAE
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
PELIHRDNLILV
CCEEECCCEEEC
>Mature Secondary Structure
MRSKVTGAQRWVVKIGSALLTADGKGLDRNAMGVWVEQMVALHEAGVELVLVSSGAVAAG
CCCCCCHHHHHHHHHCCHHEECCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCHHHH
MSRLGWTARPSAMHELQAAAAIGQMGLVQAWESSFAEHGRHTAQILLTHDDLSDRKRYLN
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHH
ARSTLRTLVELGVVPVINENDTVVTDEIRFGDNDTLAALVANLVEADLLVILTDRDGMFD
HHHHHHHHHHHCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCC
ADPRNNPEAQLIYEARADDPALDAVAGGTGGALGRGGMQTKLRAARLAARSGAHTVIVGG
CCCCCCCCEEEEEEECCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECC
RIERVLARLKGGERLGTLLSPERGMLAARKQWLAGHLQTRGTLVLDDGAVAALARDQKSL
HHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEHHHCCHHH
LPVGVKLVQGSFRRGEMVVCVAQDGREIARGLSNYSAIEAQKIIGHSSESIVRELGYMAE
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
PELIHRDNLILV
CCEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA