Definition Pseudomonas syringae pv. syringae B728a, complete genome.
Accession NC_007005
Length 6,093,698

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The map label for this gene is selU [H]

Identifier: 66043964

GI number: 66043964

Start: 801532

End: 802632

Strand: Direct

Name: selU [H]

Synonym: Psyr_0697

Alternate gene names: 66043964

Gene position: 801532-802632 (Clockwise)

Preceding gene: 66043963

Following gene: 66043966

Centisome position: 13.15

GC content: 60.58

Gene sequence:

>1101_bases
ATGGCCGACAACAGCAGCGACTACCGCGCACTGTTTCTCAACGACGTGCCGATGATGGACGCTCGGGCTCCGGTGGAGTT
TTCCAAGGGGGCTTTCCCAGGCGTGATCAACCTGCCGCTGATGAATGACATTGAGCGGCAAAAGGTCGGCACCTGCTACA
AGCAACACGGTCAGGACGCCGCTATCCAGCTCGGGCATCAATTGGTCTGCGGTCAGGTCAAGGATGAACGCGTCAATGCG
TGGATCGAGTTCGCCAAAGCCAATCCGGATGGCTACCTGTACTGCTTTCGCGGCGGGCTGCGCTCGCAGACCGTGCAGCG
CTGGCTGAAGGATGCCGGGGTCGACTACCCGCGGGTTCTTGGCGGCTACAAGGCGATGCGCACGTTCCTGCTGGATACCC
TGCACGAGGCGGTGAGCGAATGCAGTATCGTCGTGCTCGGCGGGATGACCGGCACGGGCAAGACCGAAGTATTGACGCAA
CTGCGCAACAGCGTGGACCTGGAAGGCATCGCCAATCACCGCGGCTCCAGCTTCGGCAAGCGTGCCACCGGGCAGCCGGC
GCAGATCGACTTCGAGAACCGCCTGGCCATCGACCTGCTCAAGAAGCGCGCAGCCGGTATCGAGCAATTCGTTGTGGAAG
ATGAAAGCCGTCTGGTTGGCAGTTGCAACGTGCCGCTGGAACTGCACCAGGCGATGCAGGGCTGCCCGATGGTCTGGCTG
GAAGACAGCTTCGAGAACCGCGTCGAGCGCATTCTCGAGGACTACGTGATCAACCTCTGCGCAGAGTTCATCACCGTCAA
GGGCGAAGAGCAGGGCTTTGGCCTGTTCGCCGAGCGTTTGTTGCAAAGCCTGAACAATATTCACAAACGTCTGGGCGGCG
AGCGTCATCAGCGCCTGTCGGACCTGATGCAGGCGGCGCTGGAAGAACAGCAGCGTAGCGGTGCGGTTGACCTGCACCGA
GGCTGGATCGAAGGGTTGCTCGGCGAATATTACGACCCGATGTACGCTTACCAGCGCGAACACAAAGCGGCGCGTATCGA
ATTCGCAGGTGATCAAACGCAAGTGCTGGCCTATCTGCGCGAGCGCTCTGAGCAACGTTAA

Upstream 100 bases:

>100_bases
GTGAAGCCGAGTTTCTGATCGTGGCCGCCGAACTGGGGCTGCTGCTCACCCCTATCGGCACCCTGCTCGAGCGACAGACC
CACGCCGTCGAGGTGTTCTG

Downstream 100 bases:

>100_bases
GCCTCGACCTCTATCCATCTGGCAAGGGACGCCATGCACTCGATTCTCGACTTCTATCAAACCCTGGGCTGGGGCCTTTC
GGCGCTGGTGATCGGCACCT

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MADNSSDYRALFLNDVPMMDARAPVEFSKGAFPGVINLPLMNDIERQKVGTCYKQHGQDAAIQLGHQLVCGQVKDERVNA
WIEFAKANPDGYLYCFRGGLRSQTVQRWLKDAGVDYPRVLGGYKAMRTFLLDTLHEAVSECSIVVLGGMTGTGKTEVLTQ
LRNSVDLEGIANHRGSSFGKRATGQPAQIDFENRLAIDLLKKRAAGIEQFVVEDESRLVGSCNVPLELHQAMQGCPMVWL
EDSFENRVERILEDYVINLCAEFITVKGEEQGFGLFAERLLQSLNNIHKRLGGERHQRLSDLMQAALEEQQRSGAVDLHR
GWIEGLLGEYYDPMYAYQREHKAARIEFAGDQTQVLAYLRERSEQR

Sequences:

>Translated_366_residues
MADNSSDYRALFLNDVPMMDARAPVEFSKGAFPGVINLPLMNDIERQKVGTCYKQHGQDAAIQLGHQLVCGQVKDERVNA
WIEFAKANPDGYLYCFRGGLRSQTVQRWLKDAGVDYPRVLGGYKAMRTFLLDTLHEAVSECSIVVLGGMTGTGKTEVLTQ
LRNSVDLEGIANHRGSSFGKRATGQPAQIDFENRLAIDLLKKRAAGIEQFVVEDESRLVGSCNVPLELHQAMQGCPMVWL
EDSFENRVERILEDYVINLCAEFITVKGEEQGFGLFAERLLQSLNNIHKRLGGERHQRLSDLMQAALEEQQRSGAVDLHR
GWIEGLLGEYYDPMYAYQREHKAARIEFAGDQTQVLAYLRERSEQR
>Mature_365_residues
ADNSSDYRALFLNDVPMMDARAPVEFSKGAFPGVINLPLMNDIERQKVGTCYKQHGQDAAIQLGHQLVCGQVKDERVNAW
IEFAKANPDGYLYCFRGGLRSQTVQRWLKDAGVDYPRVLGGYKAMRTFLLDTLHEAVSECSIVVLGGMTGTGKTEVLTQL
RNSVDLEGIANHRGSSFGKRATGQPAQIDFENRLAIDLLKKRAAGIEQFVVEDESRLVGSCNVPLELHQAMQGCPMVWLE
DSFENRVERILEDYVINLCAEFITVKGEEQGFGLFAERLLQSLNNIHKRLGGERHQRLSDLMQAALEEQQRSGAVDLHRG
WIEGLLGEYYDPMYAYQREHKAARIEFAGDQTQVLAYLRERSEQR

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=360, Percent_Identity=45.8333333333333, Blast_Score=323, Evalue=8e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 41261; Mature: 41130

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNSSDYRALFLNDVPMMDARAPVEFSKGAFPGVINLPLMNDIERQKVGTCYKQHGQDA
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCHH
AIQLGHQLVCGQVKDERVNAWIEFAKANPDGYLYCFRGGLRSQTVQRWLKDAGVDYPRVL
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHH
GGYKAMRTFLLDTLHEAVSECSIVVLGGMTGTGKTEVLTQLRNSVDLEGIANHRGSSFGK
HHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCC
RATGQPAQIDFENRLAIDLLKKRAAGIEQFVVEDESRLVGSCNVPLELHQAMQGCPMVWL
CCCCCCCEECCCHHHHHHHHHHHHCCHHHHHHCCCHHHEECCCCCHHHHHHHCCCCEEEE
EDSFENRVERILEDYVINLCAEFITVKGEEQGFGLFAERLLQSLNNIHKRLGGERHQRLS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
DLMQAALEEQQRSGAVDLHRGWIEGLLGEYYDPMYAYQREHKAARIEFAGDQTQVLAYLR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCHHHHHHHHH
ERSEQR
HHHCCC
>Mature Secondary Structure 
ADNSSDYRALFLNDVPMMDARAPVEFSKGAFPGVINLPLMNDIERQKVGTCYKQHGQDA
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCHH
AIQLGHQLVCGQVKDERVNAWIEFAKANPDGYLYCFRGGLRSQTVQRWLKDAGVDYPRVL
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHH
GGYKAMRTFLLDTLHEAVSECSIVVLGGMTGTGKTEVLTQLRNSVDLEGIANHRGSSFGK
HHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCC
RATGQPAQIDFENRLAIDLLKKRAAGIEQFVVEDESRLVGSCNVPLELHQAMQGCPMVWL
CCCCCCCEECCCHHHHHHHHHHHHCCHHHHHHCCCHHHEECCCCCHHHHHHHCCCCEEEE
EDSFENRVERILEDYVINLCAEFITVKGEEQGFGLFAERLLQSLNNIHKRLGGERHQRLS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
DLMQAALEEQQRSGAVDLHRGWIEGLLGEYYDPMYAYQREHKAARIEFAGDQTQVLAYLR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCHHHHHHHHH
ERSEQR
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA