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The map label for this gene is purA [H]

Identifier: 66043844

GI number: 66043844

Start: 655874

End: 657166

Strand: Direct

Name: purA [H]

Synonym: Psyr_0577

Alternate gene names: 66043844

Gene position: 655874-657166 (Clockwise)

Preceding gene: 66043843

Following gene: 66043845

Centisome position: 10.76

GC content: 60.56

Gene sequence:

>1293_bases
ATGGGTAAGAATGTCGTAGTCCTGGGCACTCAATGGGGTGATGAGGGCAAAGGCAAGATCGTTGATCTGCTGACCGAACA
TGCTACCGCCGTAGTTCGCTATCAGGGTGGCCACAACGCGGGCCACACGCTGGTGATCGACGGTGAGAAAACCGTTTTGC
ACCTGATTCCGTCCGGTGTGCTGCGCGAAGGCGTGCAGTGCCTGATCGGTAACGGTGTGGTGGTTGCTCCCGACGCGCTC
CTGCGCGAAATCGTCAAGCTGGAAGAGAAAGGCATTCCGGTGCGCGAGCGTCTGCGCATCAGCCCGTCCTGCCCGCTGAT
CCTGTCGTACCACGTGGCACTCGACCAGGCGCGTGAAAAAGCCCGCGGCGAGTTCAAGATCGGCACCACCGGTCGTGGTA
TCGGCCCGGCCTACGAAGACAAGGTCGCACGTCGCGGTCTGCGCATCGGTGACCTGTTCCATCGCGAGCGTTTCGCCGCC
AAGCTGGGCGAACTGCTCGACTATCACAACTTCGTGCTGGTCAATTACTACAAAGAGCCTGCCATCGACTTCCAGAAGAC
GCTCGACGAGTGCATGGAATACGCTGACATGCTCAAGCCGCTGATGCTCGACGTCACCGCTGCGCTGCATGAGATGCGTC
GCGACGGCAAGGACATCATGTTCGAAGGCGCCCAGGGTTCGCTGCTGGACATCGACCACGGTACTTACCCGTACGTCACC
AGCTCCAACACCACGGCGGGCGGCATTGCCACCGGCTCCGGTTTTGGCCCGATGTACCTGGATTACATCCTGGGCATCAC
CAAGGCTTACACCACACGTGTGGGTTCGGGTCCGTTCCCGACAGAACTGTTCGACGACGTCGGTGCGTTCCTGGCCAAGC
GTGGTCATGAGTTCGGTGCCACCACCGGTCGTGCACGCCGTTGTGGCTGGTTCGATGCCGTCATCCTGCGTCGCGCCATC
GAAATCAACAGCATCTCGGGCCTGTGCCTGACCAAGCTGGACGTGCTCGACGGTCTGGAAACCATCAACATCTGCGTGGG
CTACGAGAACGAAGAGGGTGCAGTCATTGACGCGCCTACCGACGCTGACAGCTATCTGGGTCTGCGTCCGGTCTACGAGC
AGATGCCAGGCTGGAGCGAATCCACGCTGGGTGCCAAGACCCTCGAAGAGCTGCCTGCTGCCGCTCGCGCTTACATCAAG
CGTGTCGAAGAGCTGGTGGGTGCGCCTATCGACATCATTTCGACCGGTCCTGACCGCAACGAAACCATCGTGCTGCGTCA
TCCGTTCGGCTGA

Upstream 100 bases:

>100_bases
GATTCAGCATGGCGAGCACTGGCAGGTAATGCCGCTGGCTTCTTGAGTATTCCTGCCGGCCGCCGCCGGCACCAAGTTTG
CGTGAATGAGGACAAGTGTT

Downstream 100 bases:

>100_bases
TACGTCACCGGGCTGTGCCAAAAAGGGTCGCCTGTGCGGCCCTTTTTTATTTTCGCGCCGAGGGCAGGGAAGCGCACAAA
AGTGAGACTTGTGTCACGGC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MGKNVVVLGTQWGDEGKGKIVDLLTEHATAVVRYQGGHNAGHTLVIDGEKTVLHLIPSGVLREGVQCLIGNGVVVAPDAL
LREIVKLEEKGIPVRERLRISPSCPLILSYHVALDQAREKARGEFKIGTTGRGIGPAYEDKVARRGLRIGDLFHRERFAA
KLGELLDYHNFVLVNYYKEPAIDFQKTLDECMEYADMLKPLMLDVTAALHEMRRDGKDIMFEGAQGSLLDIDHGTYPYVT
SSNTTAGGIATGSGFGPMYLDYILGITKAYTTRVGSGPFPTELFDDVGAFLAKRGHEFGATTGRARRCGWFDAVILRRAI
EINSISGLCLTKLDVLDGLETINICVGYENEEGAVIDAPTDADSYLGLRPVYEQMPGWSESTLGAKTLEELPAAARAYIK
RVEELVGAPIDIISTGPDRNETIVLRHPFG

Sequences:

>Translated_430_residues
MGKNVVVLGTQWGDEGKGKIVDLLTEHATAVVRYQGGHNAGHTLVIDGEKTVLHLIPSGVLREGVQCLIGNGVVVAPDAL
LREIVKLEEKGIPVRERLRISPSCPLILSYHVALDQAREKARGEFKIGTTGRGIGPAYEDKVARRGLRIGDLFHRERFAA
KLGELLDYHNFVLVNYYKEPAIDFQKTLDECMEYADMLKPLMLDVTAALHEMRRDGKDIMFEGAQGSLLDIDHGTYPYVT
SSNTTAGGIATGSGFGPMYLDYILGITKAYTTRVGSGPFPTELFDDVGAFLAKRGHEFGATTGRARRCGWFDAVILRRAI
EINSISGLCLTKLDVLDGLETINICVGYENEEGAVIDAPTDADSYLGLRPVYEQMPGWSESTLGAKTLEELPAAARAYIK
RVEELVGAPIDIISTGPDRNETIVLRHPFG
>Mature_429_residues
GKNVVVLGTQWGDEGKGKIVDLLTEHATAVVRYQGGHNAGHTLVIDGEKTVLHLIPSGVLREGVQCLIGNGVVVAPDALL
REIVKLEEKGIPVRERLRISPSCPLILSYHVALDQAREKARGEFKIGTTGRGIGPAYEDKVARRGLRIGDLFHRERFAAK
LGELLDYHNFVLVNYYKEPAIDFQKTLDECMEYADMLKPLMLDVTAALHEMRRDGKDIMFEGAQGSLLDIDHGTYPYVTS
SNTTAGGIATGSGFGPMYLDYILGITKAYTTRVGSGPFPTELFDDVGAFLAKRGHEFGATTGRARRCGWFDAVILRRAIE
INSISGLCLTKLDVLDGLETINICVGYENEEGAVIDAPTDADSYLGLRPVYEQMPGWSESTLGAKTLEELPAAARAYIKR
VEELVGAPIDIISTGPDRNETIVLRHPFG

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=42.4882629107981, Blast_Score=328, Evalue=5e-90,
Organism=Homo sapiens, GI34577063, Length=436, Percent_Identity=41.743119266055, Blast_Score=315, Evalue=5e-86,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=40.5541561712846, Blast_Score=288, Evalue=6e-78,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=69.5348837209302, Blast_Score=627, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25146553, Length=431, Percent_Identity=41.9953596287703, Blast_Score=335, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI25146555, Length=431, Percent_Identity=41.9953596287703, Blast_Score=334, Evalue=5e-92,
Organism=Saccharomyces cerevisiae, GI6324109, Length=429, Percent_Identity=41.7249417249417, Blast_Score=331, Evalue=1e-91,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=42.1176470588235, Blast_Score=336, Evalue=2e-92,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46975; Mature: 46844

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNVVVLGTQWGDEGKGKIVDLLTEHATAVVRYQGGHNAGHTLVIDGEKTVLHLIPSGV
CCCCEEEEEECCCCCCCCHHHHHHHHHCEEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LREGVQCLIGNGVVVAPDALLREIVKLEEKGIPVRERLRISPSCPLILSYHVALDQAREK
HHHHHHEEECCCEEECCHHHHHHHHHHHHCCCCHHHHEECCCCCCEEEHHHHHHHHHHHH
ARGEFKIGTTGRGIGPAYEDKVARRGLRIGDLFHRERFAAKLGELLDYHNFVLVNYYKEP
HCCCEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
AIDFQKTLDECMEYADMLKPLMLDVTAALHEMRRDGKDIMFEGAQGSLLDIDHGTYPYVT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSNTTAGGIATGSGFGPMYLDYILGITKAYTTRVGSGPFPTELFDDVGAFLAKRGHEFGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCC
TTGRARRCGWFDAVILRRAIEINSISGLCLTKLDVLDGLETINICVGYENEEGAVIDAPT
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCEEECCC
DADSYLGLRPVYEQMPGWSESTLGAKTLEELPAAARAYIKRVEELVGAPIDIISTGPDRN
CCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
ETIVLRHPFG
CEEEEECCCC
>Mature Secondary Structure 
GKNVVVLGTQWGDEGKGKIVDLLTEHATAVVRYQGGHNAGHTLVIDGEKTVLHLIPSGV
CCCEEEEEECCCCCCCCHHHHHHHHHCEEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LREGVQCLIGNGVVVAPDALLREIVKLEEKGIPVRERLRISPSCPLILSYHVALDQAREK
HHHHHHEEECCCEEECCHHHHHHHHHHHHCCCCHHHHEECCCCCCEEEHHHHHHHHHHHH
ARGEFKIGTTGRGIGPAYEDKVARRGLRIGDLFHRERFAAKLGELLDYHNFVLVNYYKEP
HCCCEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
AIDFQKTLDECMEYADMLKPLMLDVTAALHEMRRDGKDIMFEGAQGSLLDIDHGTYPYVT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSNTTAGGIATGSGFGPMYLDYILGITKAYTTRVGSGPFPTELFDDVGAFLAKRGHEFGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCC
TTGRARRCGWFDAVILRRAIEINSISGLCLTKLDVLDGLETINICVGYENEEGAVIDAPT
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCEEECCC
DADSYLGLRPVYEQMPGWSESTLGAKTLEELPAAARAYIKRVEELVGAPIDIISTGPDRN
CCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
ETIVLRHPFG
CEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA