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The map label for this gene is rfaP [H]

Identifier: 66043790

GI number: 66043790

Start: 585620

End: 586426

Strand: Direct

Name: rfaP [H]

Synonym: Psyr_0523

Alternate gene names: 66043790

Gene position: 585620-586426 (Clockwise)

Preceding gene: 66043789

Following gene: 66043791

Centisome position: 9.61

GC content: 61.59

Gene sequence:

>807_bases
ATGAAGCTGTTCCTTGCTGAGCCGTTCAAGAGCCTGTGGGCGGAGCGCGATGCGTTCGTCGAGGTCGAAGGCCTGAGCGG
TGAGGTCTACCGCGAACTGGAAGGGCGCCGCACGCTGCGCACCGAGGTCGACGGGCGCGGTTACTTCGTCAAGATTCACC
GCGGCATTGGCTGGGGCGAGATCGCCAAGAACCTGGCAACCGCCAAGCTGCCGGTTCTGGGCGCGGGCAAGGAATGGGAC
GCCATCGAGCGTCTGCATGAAGTCGGCGTCCCGACCATGACCGCCGTGGCCTATGGTGAGCGTGGCAGCAACCCTGCCGC
GCAGCACTCGTTCATCGTCACTGAAGAACTGGCACCGACCATCAGCCTTGAAGACATCAGCCTCAACTGGCGCAGCGAGC
CGCCCGAGCCGCGTCTGAAAAGAGCCTTCATTGCCGAAGTCGCCCGCATGGTCGGCATGATGCACCGCGCAGGCGTCAAT
CATCGCGACTGCTACATCTGCCATTTTCTGCTGCACACCGACAAGCCAGTGACCGCTGACGATTTCAAACTGTCGGTCAT
CGACCTGCACCGTGCCCAGACCCGCCGTGCGATCACGCCGCGCTGGCGCAACAAGGACCTGGCCGCGCTGTATTTCTCCG
CGCTGGACATCGGCCTGACCCGCCGCGACAAACTGCGTTTTCTCAAGGGTTATTTCCAGAAGCCGTTGCGCGAAATCCTG
CTCAAGGAAGCGACGTTGCTGACCTGGCTGGATAAAAAAGCCGACAAGTTGTATCAGCGCAAAGTGCGCTACGGAGACGC
TCTCTGA

Upstream 100 bases:

>100_bases
GCAGCGCGTGGTCGCGCAACGGCCTGACCTTTGCCGACACGGCAGACCTTTACAGCATGCCGCAACACGCTGCGGATGTG
ATTCTGGCGGAGCACTATTG

Downstream 100 bases:

>100_bases
TGGCGGGCTGGAAGCTCGAGCCCGAGTACGCGTTCCTGGAACAGGAATTCGGTAGCCTGGATGCCGTATTTGCCCTGCAG
GGACAGCAGTTGACCCGCGA

Product: lipopolysaccharide kinase

Products: NA

Alternate protein names: Lipopolysaccharide kinase WaaP [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKLFLAEPFKSLWAERDAFVEVEGLSGEVYRELEGRRTLRTEVDGRGYFVKIHRGIGWGEIAKNLATAKLPVLGAGKEWD
AIERLHEVGVPTMTAVAYGERGSNPAAQHSFIVTEELAPTISLEDISLNWRSEPPEPRLKRAFIAEVARMVGMMHRAGVN
HRDCYICHFLLHTDKPVTADDFKLSVIDLHRAQTRRAITPRWRNKDLAALYFSALDIGLTRRDKLRFLKGYFQKPLREIL
LKEATLLTWLDKKADKLYQRKVRYGDAL

Sequences:

>Translated_268_residues
MKLFLAEPFKSLWAERDAFVEVEGLSGEVYRELEGRRTLRTEVDGRGYFVKIHRGIGWGEIAKNLATAKLPVLGAGKEWD
AIERLHEVGVPTMTAVAYGERGSNPAAQHSFIVTEELAPTISLEDISLNWRSEPPEPRLKRAFIAEVARMVGMMHRAGVN
HRDCYICHFLLHTDKPVTADDFKLSVIDLHRAQTRRAITPRWRNKDLAALYFSALDIGLTRRDKLRFLKGYFQKPLREIL
LKEATLLTWLDKKADKLYQRKVRYGDAL
>Mature_268_residues
MKLFLAEPFKSLWAERDAFVEVEGLSGEVYRELEGRRTLRTEVDGRGYFVKIHRGIGWGEIAKNLATAKLPVLGAGKEWD
AIERLHEVGVPTMTAVAYGERGSNPAAQHSFIVTEELAPTISLEDISLNWRSEPPEPRLKRAFIAEVARMVGMMHRAGVN
HRDCYICHFLLHTDKPVTADDFKLSVIDLHRAQTRRAITPRWRNKDLAALYFSALDIGLTRRDKLRFLKGYFQKPLREIL
LKEATLLTWLDKKADKLYQRKVRYGDAL

Specific function: Catalyzes the phosphorylation of heptose(I) of the outer membrane lipopolysaccharide core. The phosphorylation of the lipopolysaccharide core seems to occur prior to translocation to the periplasm and attachment of O-antigen. Also has protein- tyrosine ki

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the protein kinase superfamily. KdkA/rfaP family [H]

Homologues:

Organism=Escherichia coli, GI1790060, Length=261, Percent_Identity=55.1724137931034, Blast_Score=296, Evalue=1e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR017172
- InterPro:   IPR010440 [H]

Pfam domain/function: PF06293 Kdo [H]

EC number: =2.7.10.2 [H]

Molecular weight: Translated: 30729; Mature: 30729

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFLAEPFKSLWAERDAFVEVEGLSGEVYRELEGRRTLRTEVDGRGYFVKIHRGIGWGE
CCEEECCHHHHHHCCCCCEEEEECCCHHHHHHHHCCHHHHEEECCCEEEEEEECCCCHHH
IAKNLATAKLPVLGAGKEWDAIERLHEVGVPTMTAVAYGERGSNPAAQHSFIVTEELAPT
HHHHHHHHHCCEEECCCCHHHHHHHHHCCCCCEEHEEECCCCCCCCHHCEEEEEHHCCCE
ISLEDISLNWRSEPPEPRLKRAFIAEVARMVGMMHRAGVNHRDCYICHFLLHTDKPVTAD
EEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCC
DFKLSVIDLHRAQTRRAITPRWRNKDLAALYFSALDIGLTRRDKLRFLKGYFQKPLREIL
CCEEEEEEHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LKEATLLTWLDKKADKLYQRKVRYGDAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLFLAEPFKSLWAERDAFVEVEGLSGEVYRELEGRRTLRTEVDGRGYFVKIHRGIGWGE
CCEEECCHHHHHHCCCCCEEEEECCCHHHHHHHHCCHHHHEEECCCEEEEEEECCCCHHH
IAKNLATAKLPVLGAGKEWDAIERLHEVGVPTMTAVAYGERGSNPAAQHSFIVTEELAPT
HHHHHHHHHCCEEECCCCHHHHHHHHHCCCCCEEHEEECCCCCCCCHHCEEEEEHHCCCE
ISLEDISLNWRSEPPEPRLKRAFIAEVARMVGMMHRAGVNHRDCYICHFLLHTDKPVTAD
EEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCC
DFKLSVIDLHRAQTRRAITPRWRNKDLAALYFSALDIGLTRRDKLRFLKGYFQKPLREIL
CCEEEEEEHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LKEATLLTWLDKKADKLYQRKVRYGDAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]