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The map label for this gene is rfaF [H]

Identifier: 66043787

GI number: 66043787

Start: 582398

End: 583432

Strand: Direct

Name: rfaF [H]

Synonym: Psyr_0520

Alternate gene names: 66043787

Gene position: 582398-583432 (Clockwise)

Preceding gene: 66043786

Following gene: 66043788

Centisome position: 9.56

GC content: 63.96

Gene sequence:

>1035_bases
ATGAAAATTCTGATCGTTGGGCCTAGCTGGGTCGGTGACATGGTGATGGCACAAACGCTGTTCCAGTGTCTGAAACAGCG
TCATCCCGAGTGCGAAATCGACGTGCTGGCCCCTGAATGGAGCCGTCCGATACTGGAGCGCATGCCGCAGGTTCGCGCTG
CGCTGAGTTTTCCGCTGGGGCACGGTGCCCTGGAACTGGCGACCCGTCGCCGGATCGGCAAGTCCCTGGCTGGCCAATAC
GATCAGGCTATCCTGCTGCCCAATTCGCTGAAATCCGCGCTGGTGCCGTTTTTCGCCGGTATCCCGAAACGTACCGGCTG
GCGCGGCGAGTTTCGCTACGGCCTGCTCAACGACGTGCGCATCCTCGACAAGCAGCGCTACCCGCTGATGATCGAACGTT
TCATGGCCCTGGCCTTCGACAAGGGCGCCGAGTTGCCGCGTCCTTACCCGCGACCGAGCCTGCGCATCGACTCGACCACG
CGCGATGCCGCACTGAGCAAATTCGGCCTCACCCTGGATCGCCCGGTACTGGCGCTGTGCCCCGGCGCAGAGTTCGGCGA
GTCCAAGCGCTGGCCTGCCGAACATTACGCACAGGTCGCTGACGCGAGCATTCGCGAAGGCTGGCAGGTCTGGCTGTTTG
GCTCGAAAAAGGATCATCCGGTCGGTGAGAGCATTCGCCAGGAGCTGATTCCCGGCCTGCGTGAGGAGTCCGTCAACCTC
AGTGGCGAGACCTCGCTGGCCGAAGCCATTGACCTGCTGTCGTGCGCCGACGCGGTGGTCTCCAACGATTCCGGCCTGAT
GCATGTGGCCGCCGCGCTGAACCGTCCGCTGGTGGCTGTCTACGGCTCGACCTCGCCGGGCTTCACGCCGCCGCTGGCCG
ACGCAGTGGAGGTGGTGCGACTGGGCATCGAGTGCAGCCCGTGCTTTGAGCGCACCTGCCGTTTCGGCCATTACAACTGC
ATGCGTCTGCTTGAGCCGGACGCCGTGACTCAGGCGCTGAGCCGTCTGAGTAGCACGCCGGTGGAGGTTGCCTGA

Upstream 100 bases:

>100_bases
ACACTCTATAAAGCTATGACAGGGAGGCGACAGGTTGTGTGGGAACGTCACGCAGTCTGATCGGCCTCCCTGATCGTTTC
TGGTCGTTTTTGGATAGAAC

Downstream 100 bases:

>100_bases
TTTTGCGGGTACTGCTGATCAAGACGTCTTCACTGGGTGATGTGATCCATGCCTTGCCCGCGCTGACCGACGCGGCGCGC
GCGCTGCCCGGTATTCGCTT

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKILIVGPSWVGDMVMAQTLFQCLKQRHPECEIDVLAPEWSRPILERMPQVRAALSFPLGHGALELATRRRIGKSLAGQY
DQAILLPNSLKSALVPFFAGIPKRTGWRGEFRYGLLNDVRILDKQRYPLMIERFMALAFDKGAELPRPYPRPSLRIDSTT
RDAALSKFGLTLDRPVLALCPGAEFGESKRWPAEHYAQVADASIREGWQVWLFGSKKDHPVGESIRQELIPGLREESVNL
SGETSLAEAIDLLSCADAVVSNDSGLMHVAAALNRPLVAVYGSTSPGFTPPLADAVEVVRLGIECSPCFERTCRFGHYNC
MRLLEPDAVTQALSRLSSTPVEVA

Sequences:

>Translated_344_residues
MKILIVGPSWVGDMVMAQTLFQCLKQRHPECEIDVLAPEWSRPILERMPQVRAALSFPLGHGALELATRRRIGKSLAGQY
DQAILLPNSLKSALVPFFAGIPKRTGWRGEFRYGLLNDVRILDKQRYPLMIERFMALAFDKGAELPRPYPRPSLRIDSTT
RDAALSKFGLTLDRPVLALCPGAEFGESKRWPAEHYAQVADASIREGWQVWLFGSKKDHPVGESIRQELIPGLREESVNL
SGETSLAEAIDLLSCADAVVSNDSGLMHVAAALNRPLVAVYGSTSPGFTPPLADAVEVVRLGIECSPCFERTCRFGHYNC
MRLLEPDAVTQALSRLSSTPVEVA
>Mature_344_residues
MKILIVGPSWVGDMVMAQTLFQCLKQRHPECEIDVLAPEWSRPILERMPQVRAALSFPLGHGALELATRRRIGKSLAGQY
DQAILLPNSLKSALVPFFAGIPKRTGWRGEFRYGLLNDVRILDKQRYPLMIERFMALAFDKGAELPRPYPRPSLRIDSTT
RDAALSKFGLTLDRPVLALCPGAEFGESKRWPAEHYAQVADASIREGWQVWLFGSKKDHPVGESIRQELIPGLREESVNL
SGETSLAEAIDLLSCADAVVSNDSGLMHVAAALNRPLVAVYGSTSPGFTPPLADAVEVVRLGIECSPCFERTCRFGHYNC
MRLLEPDAVTQALSRLSSTPVEVA

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=342, Percent_Identity=52.046783625731, Blast_Score=363, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 38008; Mature: 38008

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIVGPSWVGDMVMAQTLFQCLKQRHPECEIDVLAPEWSRPILERMPQVRAALSFPLG
CEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCC
HGALELATRRRIGKSLAGQYDQAILLPNSLKSALVPFFAGIPKRTGWRGEFRYGLLNDVR
CCHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHH
ILDKQRYPLMIERFMALAFDKGAELPRPYPRPSLRIDSTTRDAALSKFGLTLDRPVLALC
HHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCCCCEEEC
PGAEFGESKRWPAEHYAQVADASIREGWQVWLFGSKKDHPVGESIRQELIPGLREESVNL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHCCCC
SGETSLAEAIDLLSCADAVVSNDSGLMHVAAALNRPLVAVYGSTSPGFTPPLADAVEVVR
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHH
LGIECSPCFERTCRFGHYNCMRLLEPDAVTQALSRLSSTPVEVA
HCCCCCHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKILIVGPSWVGDMVMAQTLFQCLKQRHPECEIDVLAPEWSRPILERMPQVRAALSFPLG
CEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCC
HGALELATRRRIGKSLAGQYDQAILLPNSLKSALVPFFAGIPKRTGWRGEFRYGLLNDVR
CCHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHH
ILDKQRYPLMIERFMALAFDKGAELPRPYPRPSLRIDSTTRDAALSKFGLTLDRPVLALC
HHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCCCCEEEC
PGAEFGESKRWPAEHYAQVADASIREGWQVWLFGSKKDHPVGESIRQELIPGLREESVNL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHCCCC
SGETSLAEAIDLLSCADAVVSNDSGLMHVAAALNRPLVAVYGSTSPGFTPPLADAVEVVR
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHH
LGIECSPCFERTCRFGHYNCMRLLEPDAVTQALSRLSSTPVEVA
HCCCCCHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]