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The map label for this gene is putP [H]

Identifier: 66043774

GI number: 66043774

Start: 562015

End: 563574

Strand: Direct

Name: putP [H]

Synonym: Psyr_0507

Alternate gene names: 66043774

Gene position: 562015-563574 (Clockwise)

Preceding gene: 66043771

Following gene: 66043776

Centisome position: 9.22

GC content: 59.29

Gene sequence:

>1560_bases
ATGTGCCGAATATCGGTCGCTATTCCCTTTGGCATCGGGTGCCAACATAAAAACAATGCCAAGGCAGAACTCATGAGTGT
CAGCAACCCTACCTTGATCACGTTCGTGATCTATATAGCGGCAATGGTATTGATCGGCCTGATGGCCTATCGCTCTACCA
ACAACCTTTCGGATTACATTCTCGGCGGACGCAGCCTCGGCAGCGTGGTCACCGCTCTTTCAGCAGGAGCCTCGGACATG
AGCGGCTGGCTGTTGATGGGGTTGCCCGGTGCCATCTATATGTCCGGCCTGTCGGAAAGCTGGATCGCCATCGGCCTGAT
CATCGGCGCCTACCTGAACTGGCTGTTCGTGGCCGGCCGTCTGCGTGTGCAGACAGAGCACAACGGTGACGCACTGACCC
TGCCGGATTACTTCAGCAGCCGTTTCGAAGACAACAGTGGCCTGCTGCGGATCATTTCGGCCATCGTGATTCTGGTGTTC
TTCACCATTTATTGCGCCTCGGGCATCGTTGCGGGCGCGCGTCTGTTTGAAAGCACCTTCGGCATGTCCTATGAAACCGC
CCTGTGGGCCGGCGCTGCGGCGACCATCGCCTACACCTTCGTCGGTGGTTTCCTGGCGGTGAGCTGGACAGACACCGTGC
AGGCCACGCTGATGATCTTCGCCCTTATCCTGACGCCGGTCATCGTTCTGCTGGCAACGGGCGGCGTCGACACCACCTTT
CTGGCGATCGAAGCGAAAGACCCGACCAGCTTCGACATGTTCAAGAACACCACATTCATCGGCATCATTTCCCTGCTCGG
CTGGGGCCTGGGTTATTTCGGTCAGCCACATATCCTGGCGCGCTTCATGGCGGCCGATTCGGTCAAGTCGATTGCCAGCG
CCCGACGCATCTCCATGGCCTGGATGATCCTGTGCCTGGGCGGCACCGTGGCGGTGGGTTTCTTCGGCATCGCTTATTTT
TCCGCGCACCCTGAGGTCGCCGGGCCGGTTACCGAGAACCCGGAGCGGGTGTTCATCGAGCTGGCCAAGCTGCTGTTCAA
TCCGTGGATTGCCGGCGTGCTGTTGTCGGCCATTCTGGCTGCGGTGATGAGTACGCTGAGCTGCCAGTTGCTGGTGTGCT
CCAGCGCGTTGACCGAGGACTTCTACAAGGCTTATCTGCGCAAGGGCGCTTCGCAGGTCGAGCTGGTCTGGGTAGGTCGG
GCAATGGTGCTGCTGATCGCGATCATTGCCATTCTGCTGGCGGCCAATCCTGAAAACCGCGTGCTGGGCCTGGTGAGTTA
CGCCTGGGCCGGTTTCGGCGCAGCCTTCGGGCCTGTGGTACTGATTTCGGTACTGTGGAAGGGCATGACTCGCAACGGCG
CTCTGGCCGGGGTCATTATGGGTGCGGCCACCGTGGTGCTCTGGAAGCAATTCAGTACCATGGGGCTGTATGAAATCATC
CCCGGTTTCATCCTGGCCAGCATCTCCATCGTGGTATTCAGCCTGATCGGCAAGCCTGCTTCTGCGTCCATGCAGACCCG
TTTCCTGGCCGCCGAACAGGATTTCAAAGCCAACCGCTGA

Upstream 100 bases:

>100_bases
ATTGTTTAATAGGATTCGTTCCCGCGGTGCAACCGCCAGTCATTGATTGCCGATCCTTGCGGAGCGATCAGTCAACGCGG
TGTCGGGTGCAAAAGCAGGG

Downstream 100 bases:

>100_bases
CAGGTCTGACTGAAACACAAACGGCCCGTTCTTCTTCGTGAAGCGCGGGCCGTTGTGTATTCAGGCTGAGAGCTGCGTGC
CGCTCAGCTCTCGTTGTGAT

Product: Na+/solute symporter

Products: NA

Alternate protein names: Proline permease; Propionate transporter [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MCRISVAIPFGIGCQHKNNAKAELMSVSNPTLITFVIYIAAMVLIGLMAYRSTNNLSDYILGGRSLGSVVTALSAGASDM
SGWLLMGLPGAIYMSGLSESWIAIGLIIGAYLNWLFVAGRLRVQTEHNGDALTLPDYFSSRFEDNSGLLRIISAIVILVF
FTIYCASGIVAGARLFESTFGMSYETALWAGAAATIAYTFVGGFLAVSWTDTVQATLMIFALILTPVIVLLATGGVDTTF
LAIEAKDPTSFDMFKNTTFIGIISLLGWGLGYFGQPHILARFMAADSVKSIASARRISMAWMILCLGGTVAVGFFGIAYF
SAHPEVAGPVTENPERVFIELAKLLFNPWIAGVLLSAILAAVMSTLSCQLLVCSSALTEDFYKAYLRKGASQVELVWVGR
AMVLLIAIIAILLAANPENRVLGLVSYAWAGFGAAFGPVVLISVLWKGMTRNGALAGVIMGAATVVLWKQFSTMGLYEII
PGFILASISIVVFSLIGKPASASMQTRFLAAEQDFKANR

Sequences:

>Translated_519_residues
MCRISVAIPFGIGCQHKNNAKAELMSVSNPTLITFVIYIAAMVLIGLMAYRSTNNLSDYILGGRSLGSVVTALSAGASDM
SGWLLMGLPGAIYMSGLSESWIAIGLIIGAYLNWLFVAGRLRVQTEHNGDALTLPDYFSSRFEDNSGLLRIISAIVILVF
FTIYCASGIVAGARLFESTFGMSYETALWAGAAATIAYTFVGGFLAVSWTDTVQATLMIFALILTPVIVLLATGGVDTTF
LAIEAKDPTSFDMFKNTTFIGIISLLGWGLGYFGQPHILARFMAADSVKSIASARRISMAWMILCLGGTVAVGFFGIAYF
SAHPEVAGPVTENPERVFIELAKLLFNPWIAGVLLSAILAAVMSTLSCQLLVCSSALTEDFYKAYLRKGASQVELVWVGR
AMVLLIAIIAILLAANPENRVLGLVSYAWAGFGAAFGPVVLISVLWKGMTRNGALAGVIMGAATVVLWKQFSTMGLYEII
PGFILASISIVVFSLIGKPASASMQTRFLAAEQDFKANR
>Mature_519_residues
MCRISVAIPFGIGCQHKNNAKAELMSVSNPTLITFVIYIAAMVLIGLMAYRSTNNLSDYILGGRSLGSVVTALSAGASDM
SGWLLMGLPGAIYMSGLSESWIAIGLIIGAYLNWLFVAGRLRVQTEHNGDALTLPDYFSSRFEDNSGLLRIISAIVILVF
FTIYCASGIVAGARLFESTFGMSYETALWAGAAATIAYTFVGGFLAVSWTDTVQATLMIFALILTPVIVLLATGGVDTTF
LAIEAKDPTSFDMFKNTTFIGIISLLGWGLGYFGQPHILARFMAADSVKSIASARRISMAWMILCLGGTVAVGFFGIAYF
SAHPEVAGPVTENPERVFIELAKLLFNPWIAGVLLSAILAAVMSTLSCQLLVCSSALTEDFYKAYLRKGASQVELVWVGR
AMVLLIAIIAILLAANPENRVLGLVSYAWAGFGAAFGPVVLISVLWKGMTRNGALAGVIMGAATVVLWKQFSTMGLYEII
PGFILASISIVVFSLIGKPASASMQTRFLAAEQDFKANR

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=511, Percent_Identity=46.7710371819961, Blast_Score=441, Evalue=1e-123,
Organism=Homo sapiens, GI110835708, Length=485, Percent_Identity=23.2989690721649, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI14140236, Length=510, Percent_Identity=22.7450980392157, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1787251, Length=493, Percent_Identity=74.8478701825558, Blast_Score=733, Evalue=0.0,
Organism=Escherichia coli, GI1790503, Length=481, Percent_Identity=25.7796257796258, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI87082237, Length=449, Percent_Identity=27.6169265033408, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790113, Length=401, Percent_Identity=24.6882793017456, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI115533094, Length=421, Percent_Identity=26.1282660332542, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24645928, Length=404, Percent_Identity=26.2376237623762, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI221459584, Length=380, Percent_Identity=24.2105263157895, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI221459588, Length=372, Percent_Identity=24.7311827956989, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24640370, Length=414, Percent_Identity=21.256038647343, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 55431; Mature: 55431

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRISVAIPFGIGCQHKNNAKAELMSVSNPTLITFVIYIAAMVLIGLMAYRSTNNLSDYI
CEEEEEEEECCCCCCCCCCCCHHEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LGGRSLGSVVTALSAGASDMSGWLLMGLPGAIYMSGLSESWIAIGLIIGAYLNWLFVAGR
CCCCHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCE
LRVQTEHNGDALTLPDYFSSRFEDNSGLLRIISAIVILVFFTIYCASGIVAGARLFESTF
EEEEECCCCCEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GMSYETALWAGAAATIAYTFVGGFLAVSWTDTVQATLMIFALILTPVIVLLATGGVDTTF
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LAIEAKDPTSFDMFKNTTFIGIISLLGWGLGYFGQPHILARFMAADSVKSIASARRISMA
EEEECCCCCCHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
WMILCLGGTVAVGFFGIAYFSAHPEVAGPVTENPERVFIELAKLLFNPWIAGVLLSAILA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVMSTLSCQLLVCSSALTEDFYKAYLRKGASQVELVWVGRAMVLLIAIIAILLAANPENR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCC
VLGLVSYAWAGFGAAFGPVVLISVLWKGMTRNGALAGVIMGAATVVLWKQFSTMGLYEII
EEHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
PGFILASISIVVFSLIGKPASASMQTRFLAAEQDFKANR
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MCRISVAIPFGIGCQHKNNAKAELMSVSNPTLITFVIYIAAMVLIGLMAYRSTNNLSDYI
CEEEEEEEECCCCCCCCCCCCHHEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LGGRSLGSVVTALSAGASDMSGWLLMGLPGAIYMSGLSESWIAIGLIIGAYLNWLFVAGR
CCCCHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCE
LRVQTEHNGDALTLPDYFSSRFEDNSGLLRIISAIVILVFFTIYCASGIVAGARLFESTF
EEEEECCCCCEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GMSYETALWAGAAATIAYTFVGGFLAVSWTDTVQATLMIFALILTPVIVLLATGGVDTTF
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LAIEAKDPTSFDMFKNTTFIGIISLLGWGLGYFGQPHILARFMAADSVKSIASARRISMA
EEEECCCCCCHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
WMILCLGGTVAVGFFGIAYFSAHPEVAGPVTENPERVFIELAKLLFNPWIAGVLLSAILA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVMSTLSCQLLVCSSALTEDFYKAYLRKGASQVELVWVGRAMVLLIAIIAILLAANPENR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCC
VLGLVSYAWAGFGAAFGPVVLISVLWKGMTRNGALAGVIMGAATVVLWKQFSTMGLYEII
EEHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
PGFILASISIVVFSLIGKPASASMQTRFLAAEQDFKANR
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3302614; 8905232; 9278503; 1400239; 3325781; 3053687; 3053649; 1567896; 8119910 [H]