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The map label for this gene is lplA [H]

Identifier: 62389963

GI number: 62389963

Start: 1123284

End: 1124342

Strand: Reverse

Name: lplA [H]

Synonym: cg1222

Alternate gene names: 62389963

Gene position: 1124342-1123284 (Counterclockwise)

Preceding gene: 62389965

Following gene: 62389962

Centisome position: 34.25

GC content: 52.88

Gene sequence:

>1059_bases
ATGAATAACCATTTTGAGCTCAAAGTACCTGGTGGAAAGCTTGTCGTCGTTGATGTGACCACCGATCTGGATTCCATTGC
TGACGTGAAGATTTCCGGCGATTTCTTCCTCGAACCCGATGAGGCATTCTTCGCCCTTGGCCGGGCGCTGCAGGGGGCGT
CGGTGGGTGATAACACTGATCGTTTGCAGGCAAAGTTGGATGCAGCGTTGGCGGAATATGATGACGTTGAGCTACACGGC
TTTAGCACTGCGGATATTGCTTTAGCTGTGCGTCGGGCAGTCACCGGCGCGCAAGATTTCACCGATTATGAATGGGAAAT
CCTGCACCCAGGGGTGCTTCCTACCCCACTTAACGTTGCGTTGGATGAGCTCCTTTTGGACCAAGTTGCCAGTGGTCAGC
GTGGCCCGACGATGCGCATTTGGGATTGGGATGATCGCGCCACAGTGATCGGTAGTTTCCAGTCATATGTCAATGAAATC
AACCAAGAAGGCGTTAATGAACATGGTGTGACCGTGGTACGACGCATGTCTGGTGGCGGTGCAATGTTTATGGAGGGCGG
CAACTGCATCACCTATTCCCTGTATGCACCGGAATCTCTCGTTGCTGGTTTGAGCTATGAGCAGTCCTATGAATATTTGG
ATCGTTGGGTGATTGCTGCGCTGAAGACACACGATGTTGACGCTTGGTACGTGCCTATCAATGACATCACCTCCACCGGC
GGAAAAATCGGTGGCGCTGCACAGAAACGTCGCAGTGGCGCAGTCCTCCACCACGTGACCATGTCCTATGACATCGATGC
GGACATGATGACCCAGGTGTTGCGCATTGGAAAGGTGAAGATTTCCGACAAGGGTCTTCGCAGCGCAAAGAAGCGCGTTG
ATCCTCTGCGCCGCCAAACAGGTGCATCACGTGAGCAAATCATCGACACCCTAAAGTCCACATTCAGTGCTAGGTACGGC
GCGCAAGAAGTAGAGCTCAGCGATGAAGATTTCGCGGCAGGCCACGACCTAGTAAAAACCAAATACGCCACCGAGGAGTG
GACTAAGCGAGTTCAATAG

Upstream 100 bases:

>100_bases
AGTCGCCGCTTTTCGACGCCCGCCTGCGGCGGGATGACTGTGATGGAGGGGCGCGTCGACAAGCAAAATCTCTTTAGCAA
ATTCGGTTACTGTGGGGCGC

Downstream 100 bases:

>100_bases
TTTCTATGGATCTGCACAAGGTAGCTGCAGCGCACGCGGCGACTCTTCCCCTGAGCACCAAAGAGTTTCCTTTCGGACCC
GAGCACGAAGTGTACAAAGT

Product: lipoate-protein ligase

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MNNHFELKVPGGKLVVVDVTTDLDSIADVKISGDFFLEPDEAFFALGRALQGASVGDNTDRLQAKLDAALAEYDDVELHG
FSTADIALAVRRAVTGAQDFTDYEWEILHPGVLPTPLNVALDELLLDQVASGQRGPTMRIWDWDDRATVIGSFQSYVNEI
NQEGVNEHGVTVVRRMSGGGAMFMEGGNCITYSLYAPESLVAGLSYEQSYEYLDRWVIAALKTHDVDAWYVPINDITSTG
GKIGGAAQKRRSGAVLHHVTMSYDIDADMMTQVLRIGKVKISDKGLRSAKKRVDPLRRQTGASREQIIDTLKSTFSARYG
AQEVELSDEDFAAGHDLVKTKYATEEWTKRVQ

Sequences:

>Translated_352_residues
MNNHFELKVPGGKLVVVDVTTDLDSIADVKISGDFFLEPDEAFFALGRALQGASVGDNTDRLQAKLDAALAEYDDVELHG
FSTADIALAVRRAVTGAQDFTDYEWEILHPGVLPTPLNVALDELLLDQVASGQRGPTMRIWDWDDRATVIGSFQSYVNEI
NQEGVNEHGVTVVRRMSGGGAMFMEGGNCITYSLYAPESLVAGLSYEQSYEYLDRWVIAALKTHDVDAWYVPINDITSTG
GKIGGAAQKRRSGAVLHHVTMSYDIDADMMTQVLRIGKVKISDKGLRSAKKRVDPLRRQTGASREQIIDTLKSTFSARYG
AQEVELSDEDFAAGHDLVKTKYATEEWTKRVQ
>Mature_352_residues
MNNHFELKVPGGKLVVVDVTTDLDSIADVKISGDFFLEPDEAFFALGRALQGASVGDNTDRLQAKLDAALAEYDDVELHG
FSTADIALAVRRAVTGAQDFTDYEWEILHPGVLPTPLNVALDELLLDQVASGQRGPTMRIWDWDDRATVIGSFQSYVNEI
NQEGVNEHGVTVVRRMSGGGAMFMEGGNCITYSLYAPESLVAGLSYEQSYEYLDRWVIAALKTHDVDAWYVPINDITSTG
GKIGGAAQKRRSGAVLHHVTMSYDIDADMMTQVLRIGKVKISDKGLRSAKKRVDPLRRQTGASREQIIDTLKSTFSARYG
AQEVELSDEDFAAGHDLVKTKYATEEWTKRVQ

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Escherichia coli, GI1790846, Length=210, Percent_Identity=30, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 38799; Mature: 38799

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNHFELKVPGGKLVVVDVTTDLDSIADVKISGDFFLEPDEAFFALGRALQGASVGDNTD
CCCCEEEEECCCEEEEEEECCCCCCCCEEEECCCEEECCHHHHHHHHHHHCCCCCCCCHH
RLQAKLDAALAEYDDVELHGFSTADIALAVRRAVTGAQDFTDYEWEILHPGVLPTPLNVA
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHH
LDELLLDQVASGQRGPTMRIWDWDDRATVIGSFQSYVNEINQEGVNEHGVTVVRRMSGGG
HHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
AMFMEGGNCITYSLYAPESLVAGLSYEQSYEYLDRWVIAALKTHDVDAWYVPINDITSTG
EEEEECCCEEEEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHCCC
GKIGGAAQKRRSGAVLHHVTMSYDIDADMMTQVLRIGKVKISDKGLRSAKKRVDPLRRQT
CCCCCHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHC
GASREQIIDTLKSTFSARYGAQEVELSDEDFAAGHDLVKTKYATEEWTKRVQ
CCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHCCCHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MNNHFELKVPGGKLVVVDVTTDLDSIADVKISGDFFLEPDEAFFALGRALQGASVGDNTD
CCCCEEEEECCCEEEEEEECCCCCCCCEEEECCCEEECCHHHHHHHHHHHCCCCCCCCHH
RLQAKLDAALAEYDDVELHGFSTADIALAVRRAVTGAQDFTDYEWEILHPGVLPTPLNVA
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHH
LDELLLDQVASGQRGPTMRIWDWDDRATVIGSFQSYVNEINQEGVNEHGVTVVRRMSGGG
HHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
AMFMEGGNCITYSLYAPESLVAGLSYEQSYEYLDRWVIAALKTHDVDAWYVPINDITSTG
EEEEECCCEEEEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHCCC
GKIGGAAQKRRSGAVLHHVTMSYDIDADMMTQVLRIGKVKISDKGLRSAKKRVDPLRRQT
CCCCCHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHC
GASREQIIDTLKSTFSARYGAQEVELSDEDFAAGHDLVKTKYATEEWTKRVQ
CCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHCCCHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]