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The map label for this gene is shiA [H]

Identifier: 62389180

GI number: 62389180

Start: 298329

End: 299684

Strand: Reverse

Name: shiA [H]

Synonym: cg0340

Alternate gene names: 62389180

Gene position: 299684-298329 (Counterclockwise)

Preceding gene: 62389181

Following gene: 62389176

Centisome position: 9.13

GC content: 56.27

Gene sequence:

>1356_bases
ATGACTACATCTTCCACAGCTTCGCCGATCGCTGAATTACAAAACCTCAGCCCGAAGCAAAGAAAATCAGAATCCCGGCG
CGCGATTATATCCAGCTACTTGGGCTCCACGATCGAGTTCTATGACTTCCTGTTATATGCCGCAGCCTCCGCGACGGTAT
TCCCCGCAGTGTTCTTTACCAATCTCGATCCGCTGGCTGGAACCATTGCCGCCTACGGAACCTTCGCCGCAGGTTATTTA
GCTAGGCCACTTGGTGGAGCAATCTTCGGACACTTCGGTGACCGTCTTGGACGCAAGAAGATGCTTGTGTTGTCCATGCT
CATCATGGGTGTGGCATCCACATGCATCGGCCTTGTCCCAAGCGCTGAAATGATCGGAAGCATGGGCGCGGTAATCCTGA
TCATGCTGCGCATCTGCCAAGGCATCGCTGTCGGTGGCGAATGGGGCGGAGCTGCCCTCATGGCGCTCGAGCACTCAGAT
TCCAAGAAGCGCGGATTCGCCGCCTCCTTCACCAACGCTGGTGCACCAACCGGCGCTGCACTGGGAACCTTCGCGCTCGG
TACCGCATCTGCTGTTCTCACCCAGGAGCAATTCCTTTCATGGGGTTGGCGCATCCCATTCCTGCTCTCTTTCGTTCTGC
TGATTGTCGGCTTGGTTATCCGCGCGAAAGTTAGCGAATCACCACTGTTCGCAGCTGCAGCGGCCGCCGAAAAAGCCAAG
CCAACCGAACGTAAAGTCCCCCTCTTGCAGGTTCTTCGCCGACCAAAGGCACTGATCTTGACCATGCTTGGCGGCGCATC
AGGATTCGGACTTCAAGTTCTCTTGTCCACCTTCTCCATCAGCTACGCAACACAATCCGGCATCGAAAGATCCAGCGTCC
TCTACGCCTTCGCAGTCGCCTCAGTGTTCTCTGTCTTCTTTGTGATCCTCTTCGGTCGCGTATCCGACCTCTTCGGACGC
CGACCCGTCATGATCATCGCGCTCGTACTGTTCGTGGCCTACCTGCCGGCATTCTTCCGGATGCTCACCTCAGACAACTG
GTTCATCCTGCTCTCGGCATTCACCATCGCGCTCGCACTCCACGCCATGCTTTACGGCCCACTAGCAGCGTTTATCTCCG
AACAATTCGGAACCTCCGCGCGCTACACAGGCGCATCCCTGGGTTACCAGTTGGCCACGCTCATCGGTGCAGGATTCACC
CCAACCATCCTGGCTAGCCTCTACGCGGGACCAGGCGGCGGAACCTCTGTCACCCCAGTCATCGTCTTCCTCGCAACGAT
GTCCCTAGTGTCCATCATCGCCATCGCAATCACCAGAGAATCAAAAGACCACGATCTTTCTACTTACGAACACTAA

Upstream 100 bases:

>100_bases
GCAACGCCACAGGCAAAATCCAAAAGAACATCCTGCGAGACTTCACCATCCCCGTTTCATAAACCCCCCAACGTCACTTT
GAAAACACTTGGAGAACGCA

Downstream 100 bases:

>100_bases
GGGGACGATAGGGTCAATTTAAAAATGCCGGTTTCGCACGAAGTGCGGAACCGGCATTTTGCGTCGAAAAGCGTGCTACT
GCGCGATCTCGACGATAACC

Product: integral membrane transport protein

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MTTSSTASPIAELQNLSPKQRKSESRRAIISSYLGSTIEFYDFLLYAAASATVFPAVFFTNLDPLAGTIAAYGTFAAGYL
ARPLGGAIFGHFGDRLGRKKMLVLSMLIMGVASTCIGLVPSAEMIGSMGAVILIMLRICQGIAVGGEWGGAALMALEHSD
SKKRGFAASFTNAGAPTGAALGTFALGTASAVLTQEQFLSWGWRIPFLLSFVLLIVGLVIRAKVSESPLFAAAAAAEKAK
PTERKVPLLQVLRRPKALILTMLGGASGFGLQVLLSTFSISYATQSGIERSSVLYAFAVASVFSVFFVILFGRVSDLFGR
RPVMIIALVLFVAYLPAFFRMLTSDNWFILLSAFTIALALHAMLYGPLAAFISEQFGTSARYTGASLGYQLATLIGAGFT
PTILASLYAGPGGGTSVTPVIVFLATMSLVSIIAIAITRESKDHDLSTYEH

Sequences:

>Translated_451_residues
MTTSSTASPIAELQNLSPKQRKSESRRAIISSYLGSTIEFYDFLLYAAASATVFPAVFFTNLDPLAGTIAAYGTFAAGYL
ARPLGGAIFGHFGDRLGRKKMLVLSMLIMGVASTCIGLVPSAEMIGSMGAVILIMLRICQGIAVGGEWGGAALMALEHSD
SKKRGFAASFTNAGAPTGAALGTFALGTASAVLTQEQFLSWGWRIPFLLSFVLLIVGLVIRAKVSESPLFAAAAAAEKAK
PTERKVPLLQVLRRPKALILTMLGGASGFGLQVLLSTFSISYATQSGIERSSVLYAFAVASVFSVFFVILFGRVSDLFGR
RPVMIIALVLFVAYLPAFFRMLTSDNWFILLSAFTIALALHAMLYGPLAAFISEQFGTSARYTGASLGYQLATLIGAGFT
PTILASLYAGPGGGTSVTPVIVFLATMSLVSIIAIAITRESKDHDLSTYEH
>Mature_450_residues
TTSSTASPIAELQNLSPKQRKSESRRAIISSYLGSTIEFYDFLLYAAASATVFPAVFFTNLDPLAGTIAAYGTFAAGYLA
RPLGGAIFGHFGDRLGRKKMLVLSMLIMGVASTCIGLVPSAEMIGSMGAVILIMLRICQGIAVGGEWGGAALMALEHSDS
KKRGFAASFTNAGAPTGAALGTFALGTASAVLTQEQFLSWGWRIPFLLSFVLLIVGLVIRAKVSESPLFAAAAAAEKAKP
TERKVPLLQVLRRPKALILTMLGGASGFGLQVLLSTFSISYATQSGIERSSVLYAFAVASVFSVFFVILFGRVSDLFGRR
PVMIIALVLFVAYLPAFFRMLTSDNWFILLSAFTIALALHAMLYGPLAAFISEQFGTSARYTGASLGYQLATLIGAGFTP
TILASLYAGPGGGTSVTPVIVFLATMSLVSIIAIAITRESKDHDLSTYEH

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=423, Percent_Identity=37.1158392434988, Blast_Score=265, Evalue=4e-72,
Organism=Escherichia coli, GI1789941, Length=454, Percent_Identity=38.1057268722467, Blast_Score=245, Evalue=3e-66,
Organism=Escherichia coli, GI1790550, Length=394, Percent_Identity=33.502538071066, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1788942, Length=400, Percent_Identity=28.75, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87082404, Length=387, Percent_Identity=24.2894056847545, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082231, Length=246, Percent_Identity=23.9837398373984, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47680; Mature: 47548

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSSTASPIAELQNLSPKQRKSESRRAIISSYLGSTIEFYDFLLYAAASATVFPAVFFT
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
NLDPLAGTIAAYGTFAAGYLARPLGGAIFGHFGDRLGRKKMLVLSMLIMGVASTCIGLVP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SAEMIGSMGAVILIMLRICQGIAVGGEWGGAALMALEHSDSKKRGFAASFTNAGAPTGAA
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCEECCCCCCCCCCHH
LGTFALGTASAVLTQEQFLSWGWRIPFLLSFVLLIVGLVIRAKVSESPLFAAAAAAEKAK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
PTERKVPLLQVLRRPKALILTMLGGASGFGLQVLLSTFSISYATQSGIERSSVLYAFAVA
CCCCCCHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SVFSVFFVILFGRVSDLFGRRPVMIIALVLFVAYLPAFFRMLTSDNWFILLSAFTIALAL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHH
HAMLYGPLAAFISEQFGTSARYTGASLGYQLATLIGAGFTPTILASLYAGPGGGTSVTPV
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHH
IVFLATMSLVSIIAIAITRESKDHDLSTYEH
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TTSSTASPIAELQNLSPKQRKSESRRAIISSYLGSTIEFYDFLLYAAASATVFPAVFFT
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
NLDPLAGTIAAYGTFAAGYLARPLGGAIFGHFGDRLGRKKMLVLSMLIMGVASTCIGLVP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SAEMIGSMGAVILIMLRICQGIAVGGEWGGAALMALEHSDSKKRGFAASFTNAGAPTGAA
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCEECCCCCCCCCCHH
LGTFALGTASAVLTQEQFLSWGWRIPFLLSFVLLIVGLVIRAKVSESPLFAAAAAAEKAK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
PTERKVPLLQVLRRPKALILTMLGGASGFGLQVLLSTFSISYATQSGIERSSVLYAFAVA
CCCCCCHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SVFSVFFVILFGRVSDLFGRRPVMIIALVLFVAYLPAFFRMLTSDNWFILLSAFTIALAL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHH
HAMLYGPLAAFISEQFGTSARYTGASLGYQLATLIGAGFTPTILASLYAGPGGGTSVTPV
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHH
IVFLATMSLVSIIAIAITRESKDHDLSTYEH
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]