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The map label for this gene is ponA [H]

Identifier: 62389176

GI number: 62389176

Start: 294001

End: 296388

Strand: Reverse

Name: ponA [H]

Synonym: cg0336

Alternate gene names: 62389176

Gene position: 296388-294001 (Counterclockwise)

Preceding gene: 62389180

Following gene: 62389174

Centisome position: 9.03

GC content: 55.65

Gene sequence:

>2388_bases
GTGTCCACCACGAATTCTCTGACAAAGCTCGTTGCATCTACAGTCGCCGCTGGCGTCCTTGGTGCGCTCGCACTTGTGCC
TTTCGCTAGTCTTTCTGGCGTTGCGGTTGCGCGTACCAATGACACGATGCAGACCAACCTTTCAGATCTGACGGATGGTC
GCGGGCCGGGCGTCACGACGATTACTGATTCCACTGACCAGCCGATTGCTTATATTTATGCGCAGCGGCGGTTTGAGGTT
GGGGGTGATCAGATTTCTACGTCGATGAAGGATGCGATCGTTTCGATTGAGGATCGCAGGTTCTATGAGCATGATGGTGT
GGATTTGCAGGGCTTTGGTCGTGCAATCCTGACGAACCTGGCTGCGGGTGGCGTGGAGCAGGGTGCTTCGACGATTAACC
AGCAGTATGTGAAGAACTTCTTGCTGTTGGTGGAAGCTGATGATGAGGCGGAGCAGGCTGCTGCTGTGGAAACCTCCATC
CCTCGTAAGCTCCGTGAGATGAAGATGGCGTCTGATTTGGAAAAGACGTTGTCGAAGGATGAGATTCTGACTCGTTATCT
CAACATTGTTCCTTTTGGTAATGGTGCTTATGGTGTTGAGGCTGCGGCGCGGACGTATTTCGGTACGTCGGCTGCCGAGT
TAACCATTCCACAGTCTGCGATGCTCGCGGGCATTGTGCAGTCTTCGTCTTATCTCAATCCATACACCAATCACGATGCT
GTGTTTGAGCGTCGTAATACTGTTTTGGGCGCTATGGCTGATGCTGGCGCGATTTCCCCAGACGAGGCTTCGGCTTTCCA
GCAGGAACCTTTGGGTGTCCTGGAAACCCCGCAAGGCTTATCCAATGGTTGTATCGGCGCTGGCGATCGTGGTTTCTTCT
GCGATTACGCTCTGCAATATCTTTCTGAGCAGGGAATCACCCAAGATATGCTGGCGAAGGACTCCTACACCATCAAATTG
ACTTTGGATCCAGATGTTCAGGATGCAGCGCACAATGCGGTGTCCTCCCACGTTGATCCAACCACCCCAGGTGTCGCTGA
AGTTGTGAACGTCATTGAGCCTGGCGAGAACTCCCGCGATATTTTGGCTATTACTTCTTCCCGCAACTACGGCCTTGACC
TGGATGCTGGTGAAACGATGCTGCCTCAGGCAACGTCCCGTGTGGGTAATGGTGCCGGTTCCATTTTCAAGATCTTTACC
GCCGCTGCAGCCATTCAGCAGGGCGCTGGCCTAGACACCATGTTGGATGTTCCTTCTCGATATGAGGTCAAGGGCATGGG
CTCCGGCGGTGCCGCGAACTGTCCCGCAAATACTTACTGCGTGGAAAACGCAGGATCCTACGCGCCTCGCATGACTCTGC
AGGACGCTCTCGCGCAGTCCCCCAACACTGCATTCGTTGAAATGATCGAGCAGGTTGGCGTGGACACCGTTGTGGATCTT
TCAGTAAAGCTGGGCCTGCGAAGCTACACCGATGAAGGTTCCTTCGACGGCGAAAGCTCAATCGCGGACTACATGAAGGA
CAACAACCTCGGTTCTTACACTCTTGGACCTACCGCTGTTAACCCTCTTGAATTGTCCAATGTTGCTGCAACCATTGCAT
CCGGTGGCATGTGGTGCGAACCCAATCCCATCGCCAGCGTCCATGACCGTGAAGGCAACGAAGTCTACATTGACCGCCCT
GCATGTGAGCGCGCCATCGATGCCGAAACGGCTTCAGCTTTGGCCGTCGGCATGAGCAAGGATACGGTCAGCGGAACTGC
GGCCTCTGCAGCCAGCATGTACGGATGGTCCTTGCCAACCGCAGCGAAGACCGGTACCACCGAGTCCAACCAGTCCTCAG
CATTTATGGGCTTCAACAGCAACTTTGCCGCAGCTCCATACATCTACAATGACGGCACCTCCACCACCCCACTGTGCAGC
GGCCCCGTCCGCCAGTGCAGCAGCGGTAACCTCTTCGGCGGTAACGAACCAGCTCAAACATGGTTTAACATGGCAAGCAA
CGTCCCCGCAGCTTCGCAAGGAACACTGCCATCCAGCAGCGATTCATTCCGCCTCGGCACTTCCGGCGAACTCCTCAACC
AGGTTGTCGGCCAAAGCGAAGCCTCCGCTCGACGCACCCTCGAAGCCAAAGGCTACAAGGTCACCACGCGTTCAGTCTCC
GGCGCCGGCAGCGCGCGCGGCACCGTAGTCAGCGCAACCCCTCAGGGTGCAGTGCTTATCGACGGTGGAACCGTCATTTT
GGACATCTCCGACGGCACAAGCCCTGCCCCCGCTGCCACCAACAATGATGACAGCGACGATGGAGACACCCCTGCTCCAT
CAACAAACAACCGCGGAACAACCATTGAAGACGCCATCAATGACGCCATCAACCAGTTCTTCCGCTAG

Upstream 100 bases:

>100_bases
CCGACTGTCAGCAGTAACGCACAGTCGAATAAATAAAGATTGGCTGACATTGTTGGAGTCTTGGGTTCACGATTCCCGGG
GTTATCGCTAGGCTGTTGGA

Downstream 100 bases:

>100_bases
AAATACCTAGTTGCTCAAACGGCTCTTCACTGCGGTGGAGAGTCGTTTTCCATCTGCACGGCCAGCAGCCTTAGCGGTAG
CAGCCTTCATGATCTGGCCC

Product: penicillin-binding protein 1B

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 795; Mature: 794

Protein sequence:

>795_residues
MSTTNSLTKLVASTVAAGVLGALALVPFASLSGVAVARTNDTMQTNLSDLTDGRGPGVTTITDSTDQPIAYIYAQRRFEV
GGDQISTSMKDAIVSIEDRRFYEHDGVDLQGFGRAILTNLAAGGVEQGASTINQQYVKNFLLLVEADDEAEQAAAVETSI
PRKLREMKMASDLEKTLSKDEILTRYLNIVPFGNGAYGVEAAARTYFGTSAAELTIPQSAMLAGIVQSSSYLNPYTNHDA
VFERRNTVLGAMADAGAISPDEASAFQQEPLGVLETPQGLSNGCIGAGDRGFFCDYALQYLSEQGITQDMLAKDSYTIKL
TLDPDVQDAAHNAVSSHVDPTTPGVAEVVNVIEPGENSRDILAITSSRNYGLDLDAGETMLPQATSRVGNGAGSIFKIFT
AAAAIQQGAGLDTMLDVPSRYEVKGMGSGGAANCPANTYCVENAGSYAPRMTLQDALAQSPNTAFVEMIEQVGVDTVVDL
SVKLGLRSYTDEGSFDGESSIADYMKDNNLGSYTLGPTAVNPLELSNVAATIASGGMWCEPNPIASVHDREGNEVYIDRP
ACERAIDAETASALAVGMSKDTVSGTAASAASMYGWSLPTAAKTGTTESNQSSAFMGFNSNFAAAPYIYNDGTSTTPLCS
GPVRQCSSGNLFGGNEPAQTWFNMASNVPAASQGTLPSSSDSFRLGTSGELLNQVVGQSEASARRTLEAKGYKVTTRSVS
GAGSARGTVVSATPQGAVLIDGGTVILDISDGTSPAPAATNNDDSDDGDTPAPSTNNRGTTIEDAINDAINQFFR

Sequences:

>Translated_795_residues
MSTTNSLTKLVASTVAAGVLGALALVPFASLSGVAVARTNDTMQTNLSDLTDGRGPGVTTITDSTDQPIAYIYAQRRFEV
GGDQISTSMKDAIVSIEDRRFYEHDGVDLQGFGRAILTNLAAGGVEQGASTINQQYVKNFLLLVEADDEAEQAAAVETSI
PRKLREMKMASDLEKTLSKDEILTRYLNIVPFGNGAYGVEAAARTYFGTSAAELTIPQSAMLAGIVQSSSYLNPYTNHDA
VFERRNTVLGAMADAGAISPDEASAFQQEPLGVLETPQGLSNGCIGAGDRGFFCDYALQYLSEQGITQDMLAKDSYTIKL
TLDPDVQDAAHNAVSSHVDPTTPGVAEVVNVIEPGENSRDILAITSSRNYGLDLDAGETMLPQATSRVGNGAGSIFKIFT
AAAAIQQGAGLDTMLDVPSRYEVKGMGSGGAANCPANTYCVENAGSYAPRMTLQDALAQSPNTAFVEMIEQVGVDTVVDL
SVKLGLRSYTDEGSFDGESSIADYMKDNNLGSYTLGPTAVNPLELSNVAATIASGGMWCEPNPIASVHDREGNEVYIDRP
ACERAIDAETASALAVGMSKDTVSGTAASAASMYGWSLPTAAKTGTTESNQSSAFMGFNSNFAAAPYIYNDGTSTTPLCS
GPVRQCSSGNLFGGNEPAQTWFNMASNVPAASQGTLPSSSDSFRLGTSGELLNQVVGQSEASARRTLEAKGYKVTTRSVS
GAGSARGTVVSATPQGAVLIDGGTVILDISDGTSPAPAATNNDDSDDGDTPAPSTNNRGTTIEDAINDAINQFFR
>Mature_794_residues
STTNSLTKLVASTVAAGVLGALALVPFASLSGVAVARTNDTMQTNLSDLTDGRGPGVTTITDSTDQPIAYIYAQRRFEVG
GDQISTSMKDAIVSIEDRRFYEHDGVDLQGFGRAILTNLAAGGVEQGASTINQQYVKNFLLLVEADDEAEQAAAVETSIP
RKLREMKMASDLEKTLSKDEILTRYLNIVPFGNGAYGVEAAARTYFGTSAAELTIPQSAMLAGIVQSSSYLNPYTNHDAV
FERRNTVLGAMADAGAISPDEASAFQQEPLGVLETPQGLSNGCIGAGDRGFFCDYALQYLSEQGITQDMLAKDSYTIKLT
LDPDVQDAAHNAVSSHVDPTTPGVAEVVNVIEPGENSRDILAITSSRNYGLDLDAGETMLPQATSRVGNGAGSIFKIFTA
AAAIQQGAGLDTMLDVPSRYEVKGMGSGGAANCPANTYCVENAGSYAPRMTLQDALAQSPNTAFVEMIEQVGVDTVVDLS
VKLGLRSYTDEGSFDGESSIADYMKDNNLGSYTLGPTAVNPLELSNVAATIASGGMWCEPNPIASVHDREGNEVYIDRPA
CERAIDAETASALAVGMSKDTVSGTAASAASMYGWSLPTAAKTGTTESNQSSAFMGFNSNFAAAPYIYNDGTSTTPLCSG
PVRQCSSGNLFGGNEPAQTWFNMASNVPAASQGTLPSSSDSFRLGTSGELLNQVVGQSEASARRTLEAKGYKVTTRSVSG
AGSARGTVVSATPQGAVLIDGGTVILDISDGTSPAPAATNNDDSDDGDTPAPSTNNRGTTIEDAINDAINQFFR

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=301, Percent_Identity=34.8837209302326, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1786343, Length=565, Percent_Identity=29.0265486725664, Blast_Score=147, Evalue=3e-36,
Organism=Escherichia coli, GI1788867, Length=197, Percent_Identity=32.994923857868, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1789601, Length=178, Percent_Identity=35.3932584269663, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 82954; Mature: 82823

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTNSLTKLVASTVAAGVLGALALVPFASLSGVAVARTNDTMQTNLSDLTDGRGPGVTT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHCCHHHHCCCCCCCEEE
ITDSTDQPIAYIYAQRRFEVGGDQISTSMKDAIVSIEDRRFYEHDGVDLQGFGRAILTNL
EECCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHEEECCCEEHHCCCCCHHHHHHHHHHHH
AAGGVEQGASTINQQYVKNFLLLVEADDEAEQAAAVETSIPRKLREMKMASDLEKTLSKD
HCCCHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EILTRYLNIVPFGNGAYGVEAAARTYFGTSAAELTIPQSAMLAGIVQSSSYLNPYTNHDA
HHHHHHHHHEECCCCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCHH
VFERRNTVLGAMADAGAISPDEASAFQQEPLGVLETPQGLSNGCIGAGDRGFFCDYALQY
HHHHHHHHHEEHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LSEQGITQDMLAKDSYTIKLTLDPDVQDAAHNAVSSHVDPTTPGVAEVVNVIEPGENSRD
HHHCCCCHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCE
ILAITSSRNYGLDLDAGETMLPQATSRVGNGAGSIFKIFTAAAAIQQGAGLDTMLDVPSR
EEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCC
YEVKGMGSGGAANCPANTYCVENAGSYAPRMTLQDALAQSPNTAFVEMIEQVGVDTVVDL
EEEEECCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCEEEEH
SVKLGLRSYTDEGSFDGESSIADYMKDNNLGSYTLGPTAVNPLELSNVAATIASGGMWCE
HHHCCCHHCCCCCCCCCHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHCCCEECC
PNPIASVHDREGNEVYIDRPACERAIDAETASALAVGMSKDTVSGTAASAASMYGWSLPT
CCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHCCCCCCC
AAKTGTTESNQSSAFMGFNSNFAAAPYIYNDGTSTTPLCSGPVRQCSSGNLFGGNEPAQT
CCCCCCCCCCCCCEEEECCCCCEECCEEECCCCCCCCCCCCHHHHCCCCCCCCCCCHHHH
WFNMASNVPAASQGTLPSSSDSFRLGTSGELLNQVVGQSEASARRTLEAKGYKVTTRSVS
HHHHHHCCCCCCCCCCCCCCCCEECCCCHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECC
GAGSARGTVVSATPQGAVLIDGGTVILDISDGTSPAPAATNNDDSDDGDTPAPSTNNRGT
CCCCCCCEEEEECCCCEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TIEDAINDAINQFFR
CHHHHHHHHHHHHCC
>Mature Secondary Structure 
STTNSLTKLVASTVAAGVLGALALVPFASLSGVAVARTNDTMQTNLSDLTDGRGPGVTT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHCCHHHHCCCCCCCEEE
ITDSTDQPIAYIYAQRRFEVGGDQISTSMKDAIVSIEDRRFYEHDGVDLQGFGRAILTNL
EECCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHEEECCCEEHHCCCCCHHHHHHHHHHHH
AAGGVEQGASTINQQYVKNFLLLVEADDEAEQAAAVETSIPRKLREMKMASDLEKTLSKD
HCCCHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EILTRYLNIVPFGNGAYGVEAAARTYFGTSAAELTIPQSAMLAGIVQSSSYLNPYTNHDA
HHHHHHHHHEECCCCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCHH
VFERRNTVLGAMADAGAISPDEASAFQQEPLGVLETPQGLSNGCIGAGDRGFFCDYALQY
HHHHHHHHHEEHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LSEQGITQDMLAKDSYTIKLTLDPDVQDAAHNAVSSHVDPTTPGVAEVVNVIEPGENSRD
HHHCCCCHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCE
ILAITSSRNYGLDLDAGETMLPQATSRVGNGAGSIFKIFTAAAAIQQGAGLDTMLDVPSR
EEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCC
YEVKGMGSGGAANCPANTYCVENAGSYAPRMTLQDALAQSPNTAFVEMIEQVGVDTVVDL
EEEEECCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCEEEEH
SVKLGLRSYTDEGSFDGESSIADYMKDNNLGSYTLGPTAVNPLELSNVAATIASGGMWCE
HHHCCCHHCCCCCCCCCHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHCCCEECC
PNPIASVHDREGNEVYIDRPACERAIDAETASALAVGMSKDTVSGTAASAASMYGWSLPT
CCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHCCCCCCC
AAKTGTTESNQSSAFMGFNSNFAAAPYIYNDGTSTTPLCSGPVRQCSSGNLFGGNEPAQT
CCCCCCCCCCCCCEEEECCCCCEECCEEECCCCCCCCCCCCHHHHCCCCCCCCCCCHHHH
WFNMASNVPAASQGTLPSSSDSFRLGTSGELLNQVVGQSEASARRTLEAKGYKVTTRSVS
HHHHHHCCCCCCCCCCCCCCCCEECCCCHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECC
GAGSARGTVVSATPQGAVLIDGGTVILDISDGTSPAPAATNNDDSDDGDTPAPSTNNRGT
CCCCCCCEEEEECCCCEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TIEDAINDAINQFFR
CHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]