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The map label for this gene is purR [H]

Identifier: 62389064

GI number: 62389064

Start: 181685

End: 182680

Strand: Reverse

Name: purR [H]

Synonym: cg0210

Alternate gene names: 62389064

Gene position: 182680-181685 (Counterclockwise)

Preceding gene: 62389073

Following gene: 62389051

Centisome position: 5.56

GC content: 57.33

Gene sequence:

>996_bases
ATGAGCACATCCCGCCCCACAATTTATGACGTCGCCAAAGCCGCAGGCGTCTCCAAATCCTTGGTTTCTCTCGTGCTTCG
CGGCTCCCCCAACGTGAGCAAAGAATCCGAAGCCGCGGTCAAGACCGCGATAAAAAAGCTCAACTACCAGCCAAATCGCG
CCGCATCAGACCTTGCGGCCAAGCGCACGCAGCTCATTGCAGTGCTTATCGACGACTACTCCAACCCGTGGTTCATCGAC
CTGATTCAAAGCCTCAGCGATGTGCTCACCCCCAAGGGGTACCGACTGTCCGTCATTGACTCATTAACCTCTCAAGCCGG
CACCGATCCCATTACCAGTGCACTATCAATGCGCCCCGATGGAATCATCATCGCCCAAGACATCCCCGATTTCACTGTCC
CCGATTCCCTACCCCCATTTGTCATCGCAGGCACCAGAATCACCCAAGCCAGCACCCATGATTCAGTGGCCAACGATGAC
TTCCGGGGCGCAGAAATAGCCACAAAACACCTCATCGATCTTGGACACACCCACATCGCCCACCTACGCGTGGGAAGCGG
CGCTGGCTTACGACGCTTCGAAAGCTTTGAGGCAACCATGCGTGCACATGGCCTGGAGCCGCTTTCCAACGATTACCTCG
GCCCCGCCGTTGAGCACGCCGGGTACACCGAAACCCTCGCACTACTCAAAGAGCACCCGGAGGTCACCGCCATTTTCTCC
TCAAACGACATCACCGCCATCGGAGCACTCGGTGCCGCCCGTGAACTAGGTTTACGCGTACCTGAAGATCTATCAATAAT
CGGATATGACAACACTCCCCTCGCCCAAACCCGACTGATCAACCTCACCACCATCGACGACAACAGCATCGGCGTCGGCT
ACAACGCCGCTCTCTTGTTGCTGAGCATGCTTGATCCCGAGGCACCCCACCCGGAGATCATGCATACGTTGCAGCCCTCG
CTGATTGAACGGGGCACGTGCGCGCCACGTGGATAG

Upstream 100 bases:

>100_bases
GTGGATCCGGTATTGTGATCCACTACGCAATTGGAGCGCTCCAACACAAGCTATATTTGTTTAAATGTCCTGTCAATAGT
TCAAGAGAAAATCACAGAAG

Downstream 100 bases:

>100_bases
CTACCCCAAATACTTGGACTTCCTAATGAGGGATTACTTCGTCTCATTAGGATGATGGGTATGGACCTGCTCGAGGAACA
CAGTGTTCTTCTCAACATAA

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSTSRPTIYDVAKAAGVSKSLVSLVLRGSPNVSKESEAAVKTAIKKLNYQPNRAASDLAAKRTQLIAVLIDDYSNPWFID
LIQSLSDVLTPKGYRLSVIDSLTSQAGTDPITSALSMRPDGIIIAQDIPDFTVPDSLPPFVIAGTRITQASTHDSVANDD
FRGAEIATKHLIDLGHTHIAHLRVGSGAGLRRFESFEATMRAHGLEPLSNDYLGPAVEHAGYTETLALLKEHPEVTAIFS
SNDITAIGALGAARELGLRVPEDLSIIGYDNTPLAQTRLINLTTIDDNSIGVGYNAALLLLSMLDPEAPHPEIMHTLQPS
LIERGTCAPRG

Sequences:

>Translated_331_residues
MSTSRPTIYDVAKAAGVSKSLVSLVLRGSPNVSKESEAAVKTAIKKLNYQPNRAASDLAAKRTQLIAVLIDDYSNPWFID
LIQSLSDVLTPKGYRLSVIDSLTSQAGTDPITSALSMRPDGIIIAQDIPDFTVPDSLPPFVIAGTRITQASTHDSVANDD
FRGAEIATKHLIDLGHTHIAHLRVGSGAGLRRFESFEATMRAHGLEPLSNDYLGPAVEHAGYTETLALLKEHPEVTAIFS
SNDITAIGALGAARELGLRVPEDLSIIGYDNTPLAQTRLINLTTIDDNSIGVGYNAALLLLSMLDPEAPHPEIMHTLQPS
LIERGTCAPRG
>Mature_330_residues
STSRPTIYDVAKAAGVSKSLVSLVLRGSPNVSKESEAAVKTAIKKLNYQPNRAASDLAAKRTQLIAVLIDDYSNPWFIDL
IQSLSDVLTPKGYRLSVIDSLTSQAGTDPITSALSMRPDGIIIAQDIPDFTVPDSLPPFVIAGTRITQASTHDSVANDDF
RGAEIATKHLIDLGHTHIAHLRVGSGAGLRRFESFEATMRAHGLEPLSNDYLGPAVEHAGYTETLALLKEHPEVTAIFSS
NDITAIGALGAARELGLRVPEDLSIIGYDNTPLAQTRLINLTTIDDNSIGVGYNAALLLLSMLDPEAPHPEIMHTLQPSL
IERGTCAPRG

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=31.2312312312312, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1789202, Length=327, Percent_Identity=30.2752293577982, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1790194, Length=339, Percent_Identity=29.4985250737463, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788474, Length=339, Percent_Identity=29.2035398230088, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789068, Length=286, Percent_Identity=30.7692307692308, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI48994940, Length=344, Percent_Identity=27.906976744186, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=28.3582089552239, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790369, Length=312, Percent_Identity=28.8461538461538, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787906, Length=279, Percent_Identity=30.8243727598566, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1790715, Length=308, Percent_Identity=24.6753246753247, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787580, Length=288, Percent_Identity=27.4305555555556, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790689, Length=307, Percent_Identity=25.4071661237785, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790192, Length=221, Percent_Identity=25.3393665158371, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35419; Mature: 35287

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSRPTIYDVAKAAGVSKSLVSLVLRGSPNVSKESEAAVKTAIKKLNYQPNRAASDLAA
CCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
KRTQLIAVLIDDYSNPWFIDLIQSLSDVLTPKGYRLSVIDSLTSQAGTDPITSALSMRPD
HHHHEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCHHHHHHHCCCC
GIIIAQDIPDFTVPDSLPPFVIAGTRITQASTHDSVANDDFRGAEIATKHLIDLGHTHIA
CEEEECCCCCCCCCCCCCCEEEECCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEE
HLRVGSGAGLRRFESFEATMRAHGLEPLSNDYLGPAVEHAGYTETLALLKEHPEVTAIFS
EEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCCEEEEEC
SNDITAIGALGAARELGLRVPEDLSIIGYDNTPLAQTRLINLTTIDDNSIGVGYNAALLL
CCCEEEEHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEEEEECCCCCCCCHHHHHHH
LSMLDPEAPHPEIMHTLQPSLIERGTCAPRG
HHHHCCCCCCHHHHHHHCHHHHHCCCCCCCC
>Mature Secondary Structure 
STSRPTIYDVAKAAGVSKSLVSLVLRGSPNVSKESEAAVKTAIKKLNYQPNRAASDLAA
CCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
KRTQLIAVLIDDYSNPWFIDLIQSLSDVLTPKGYRLSVIDSLTSQAGTDPITSALSMRPD
HHHHEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCHHHHHHHCCCC
GIIIAQDIPDFTVPDSLPPFVIAGTRITQASTHDSVANDDFRGAEIATKHLIDLGHTHIA
CEEEECCCCCCCCCCCCCCEEEECCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEE
HLRVGSGAGLRRFESFEATMRAHGLEPLSNDYLGPAVEHAGYTETLALLKEHPEVTAIFS
EEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCCEEEEEC
SNDITAIGALGAARELGLRVPEDLSIIGYDNTPLAQTRLINLTTIDDNSIGVGYNAALLL
CCCEEEEHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEEEEECCCCCCCCHHHHHHH
LSMLDPEAPHPEIMHTLQPSLIERGTCAPRG
HHHHCCCCCCHHHHHHHCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA