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The map label for this gene is hsrA [H]

Identifier: 62389060

GI number: 62389060

Start: 178286

End: 179662

Strand: Direct

Name: hsrA [H]

Synonym: cg0206

Alternate gene names: 62389060

Gene position: 178286-179662 (Clockwise)

Preceding gene: 62389059

Following gene: 62389061

Centisome position: 5.43

GC content: 54.39

Gene sequence:

>1377_bases
TTGGACATCGACGCCATGCGCAATGATCGGTCCTTTAGCGTTCCCATTGCGCTACTTGCCGCGGGAGCACTGTTTCTAGA
AATCCTCGACGGCACCATCCTGACAACCGCAGTGCCAGCTATTGCTCGTGACTTCGGTATTGACGCCGTGGATGTCAGCA
TTGCACTGGTTGCTTACTTGGCAGCCGCAGCAGCTGGCATTCCGGCTGCAGGGTGGCTAGCGGATCGATTTGGTGTGCGC
AAAGTCCTGTTGCTGGCTTTAGCGGTGTTTACTGCAGCCTCCTTGGTTTGTGCAGTGGCGCCGGGGCTGACTGTGCTTAC
CGGTGCTCGCGTTATCCAAGGTCTTGGTGGTGCACTACTCGTCCCAGTCGGGCGATTAGCAGTAATCCGGGGAACGGATC
CCAAAGATCTCCTTGATGCCATCGCGTTTTTAACCTGGCCAGCTCTGGTTGCCCCAGTGATCGCCCCACTTCTGGGAGGT
CTTCTTGCCGATACCATTGGTTGGCGATGGATCTTCCTCCTCAACGTGCCCTTAGGAATCATCGCGATCATTGCTGGACT
ATTCATCCTGCCCAAGAACACTGCCGTGAATGTGAAACGATTTGATCTTCCAGGTTTCCTCGGCGCAATGCTGGTGATGG
TGGCGCTGACCGTGGCTGCGGAGTTAATTTCCAGGGGAAGTTCGGCTGAACTTACTATCGCTGCATGCCTCGTCTTAAGT
GCTGCGGTGGTATGCGGTTTTGTAGTGCGCTGGCTGCGAGTTCCAGGCCGACTTTTTGATCTCAGCATCATGCGCATCCC
AGGTTTCCGAGTGGGTAATTCCTCCGGAAGTATCTACCGCTTGGTAATCACCGCAGCACCATTCATGTTCACTTTGCTCT
TCCAAGTGGCGTTTGGGTGGTCTGCAACATTAGCGGGTGCCATGGTGGTCGCACTATTCGCAGGCAACGTGGCAATCAAA
CCTTTCACCACGCCGATCATTAAACGCTGGAATTTCAAACCAGTACTGGTCTTTTCTAACGCTGCTGGCGCCTTGGTATT
GGCAACTTTTTTGTTCGTTCGTGCAGATACCCCACTGGTTCTCATCGTGCTGCTGCTCTTTGTTTCGGGCGCATTAAGGT
CCCTCGGTTTCAGCGCCTACAACACCTTGCAGTTTGTCGATATCTCACCAGAACAAACCAGCAACGCCAACGTGTTATCA
GCAACCCTGCACCAACTAGGCATGTCTTTGGGTATTGCGGTAGCAGTCATCGCCATGTCCCTTGCACCCACCGCCAACTG
GGCATTCCCACTGGCAGCAGCGTTGTTCCTCATTCCTCTAATCGGCGCACTATCTTTGCCTCGCGACGGCGGTGCCCGAG
CCTTTTCCTCCTCTTAG

Upstream 100 bases:

>100_bases
CTCACTCATCGAAAACGCATAGAGTTATCTCGAAACTACCAAGACGGTTTCTTTTCTTTAGATCTAAGGAGGAGAGACCG
TCTTTCACCCTTTCATCTGA

Downstream 100 bases:

>100_bases
AAACCCACTTCTGAAAGGTATAAAAACATGACTATTCGAATCGGACTCGTTGGCTACGGTGTCGGCGGCAGGCTCTTTCA
CACCCCTTACATCCAAGCTT

Product: transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MDIDAMRNDRSFSVPIALLAAGALFLEILDGTILTTAVPAIARDFGIDAVDVSIALVAYLAAAAAGIPAAGWLADRFGVR
KVLLLALAVFTAASLVCAVAPGLTVLTGARVIQGLGGALLVPVGRLAVIRGTDPKDLLDAIAFLTWPALVAPVIAPLLGG
LLADTIGWRWIFLLNVPLGIIAIIAGLFILPKNTAVNVKRFDLPGFLGAMLVMVALTVAAELISRGSSAELTIAACLVLS
AAVVCGFVVRWLRVPGRLFDLSIMRIPGFRVGNSSGSIYRLVITAAPFMFTLLFQVAFGWSATLAGAMVVALFAGNVAIK
PFTTPIIKRWNFKPVLVFSNAAGALVLATFLFVRADTPLVLIVLLLFVSGALRSLGFSAYNTLQFVDISPEQTSNANVLS
ATLHQLGMSLGIAVAVIAMSLAPTANWAFPLAAALFLIPLIGALSLPRDGGARAFSSS

Sequences:

>Translated_458_residues
MDIDAMRNDRSFSVPIALLAAGALFLEILDGTILTTAVPAIARDFGIDAVDVSIALVAYLAAAAAGIPAAGWLADRFGVR
KVLLLALAVFTAASLVCAVAPGLTVLTGARVIQGLGGALLVPVGRLAVIRGTDPKDLLDAIAFLTWPALVAPVIAPLLGG
LLADTIGWRWIFLLNVPLGIIAIIAGLFILPKNTAVNVKRFDLPGFLGAMLVMVALTVAAELISRGSSAELTIAACLVLS
AAVVCGFVVRWLRVPGRLFDLSIMRIPGFRVGNSSGSIYRLVITAAPFMFTLLFQVAFGWSATLAGAMVVALFAGNVAIK
PFTTPIIKRWNFKPVLVFSNAAGALVLATFLFVRADTPLVLIVLLLFVSGALRSLGFSAYNTLQFVDISPEQTSNANVLS
ATLHQLGMSLGIAVAVIAMSLAPTANWAFPLAAALFLIPLIGALSLPRDGGARAFSSS
>Mature_458_residues
MDIDAMRNDRSFSVPIALLAAGALFLEILDGTILTTAVPAIARDFGIDAVDVSIALVAYLAAAAAGIPAAGWLADRFGVR
KVLLLALAVFTAASLVCAVAPGLTVLTGARVIQGLGGALLVPVGRLAVIRGTDPKDLLDAIAFLTWPALVAPVIAPLLGG
LLADTIGWRWIFLLNVPLGIIAIIAGLFILPKNTAVNVKRFDLPGFLGAMLVMVALTVAAELISRGSSAELTIAACLVLS
AAVVCGFVVRWLRVPGRLFDLSIMRIPGFRVGNSSGSIYRLVITAAPFMFTLLFQVAFGWSATLAGAMVVALFAGNVAIK
PFTTPIIKRWNFKPVLVFSNAAGALVLATFLFVRADTPLVLIVLLLFVSGALRSLGFSAYNTLQFVDISPEQTSNANVLS
ATLHQLGMSLGIAVAVIAMSLAPTANWAFPLAAALFLIPLIGALSLPRDGGARAFSSS

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=397, Percent_Identity=34.5088161209068, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1788392, Length=398, Percent_Identity=33.9195979899498, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789042, Length=318, Percent_Identity=29.559748427673, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1788710, Length=405, Percent_Identity=27.6543209876543, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI87081983, Length=246, Percent_Identity=35.3658536585366, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790146, Length=157, Percent_Identity=32.484076433121, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=159, Percent_Identity=28.9308176100629, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=171, Percent_Identity=29.8245614035088, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47698; Mature: 47698

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIDAMRNDRSFSVPIALLAAGALFLEILDGTILTTAVPAIARDFGIDAVDVSIALVAYL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AAAAAGIPAAGWLADRFGVRKVLLLALAVFTAASLVCAVAPGLTVLTGARVIQGLGGALL
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHE
VPVGRLAVIRGTDPKDLLDAIAFLTWPALVAPVIAPLLGGLLADTIGWRWIFLLNVPLGI
ECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
IAIIAGLFILPKNTAVNVKRFDLPGFLGAMLVMVALTVAAELISRGSSAELTIAACLVLS
HHHHHHHHEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AAVVCGFVVRWLRVPGRLFDLSIMRIPGFRVGNSSGSIYRLVITAAPFMFTLLFQVAFGW
HHHHHHHHHHHHHCCCHHEEEEEEECCCEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCH
SATLAGAMVVALFAGNVAIKPFTTPIIKRWNFKPVLVFSNAAGALVLATFLFVRADTPLV
HHHHHHHHHHHHHHCCEEECCCCHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCHHH
LIVLLLFVSGALRSLGFSAYNTLQFVDISPEQTSNANVLSATLHQLGMSLGIAVAVIAMS
HHHHHHHHHHHHHHCCHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LAPTANWAFPLAAALFLIPLIGALSLPRDGGARAFSSS
HCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MDIDAMRNDRSFSVPIALLAAGALFLEILDGTILTTAVPAIARDFGIDAVDVSIALVAYL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AAAAAGIPAAGWLADRFGVRKVLLLALAVFTAASLVCAVAPGLTVLTGARVIQGLGGALL
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHE
VPVGRLAVIRGTDPKDLLDAIAFLTWPALVAPVIAPLLGGLLADTIGWRWIFLLNVPLGI
ECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
IAIIAGLFILPKNTAVNVKRFDLPGFLGAMLVMVALTVAAELISRGSSAELTIAACLVLS
HHHHHHHHEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AAVVCGFVVRWLRVPGRLFDLSIMRIPGFRVGNSSGSIYRLVITAAPFMFTLLFQVAFGW
HHHHHHHHHHHHHCCCHHEEEEEEECCCEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCH
SATLAGAMVVALFAGNVAIKPFTTPIIKRWNFKPVLVFSNAAGALVLATFLFVRADTPLV
HHHHHHHHHHHHHHCCEEECCCCHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCHHH
LIVLLLFVSGALRSLGFSAYNTLQFVDISPEQTSNANVLSATLHQLGMSLGIAVAVIAMS
HHHHHHHHHHHHHHCCHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LAPTANWAFPLAAALFLIPLIGALSLPRDGGARAFSSS
HCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]