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The map label for this gene is tolB

Identifier: 62185544

GI number: 62185544

Start: 1105630

End: 1106913

Strand: Direct

Name: tolB

Synonym: CAB944

Alternate gene names: 62185544

Gene position: 1105630-1106913 (Clockwise)

Preceding gene: 62185543

Following gene: 62185545

Centisome position: 96.61

GC content: 40.26

Gene sequence:

>1284_bases
ATGTTACGACGTATGTTAGTCAGTGCTTTCCTGATGTTTGGGATAATCTCCCTCTATGCTAAAGAGTTAGAAGTCTCTGT
GCGTTCGGAAATATCTCGTTTACCTATACACGTAGAGTTAAAGATCGGCTCTAACGATGCGTCGCAACAAAAATATTTAC
GCTCATTGTGTACTACCTTCATTAATGATTTAGCTCTTGGTGATCGTCTTCAGCCTTCTCTTGTTCAAACCGGAAGCTCC
TCAGCGCCTTTTAATATAGCTATGGTTTCCCATTATCCGGAAATCACATTTACCTTAGCCAGAGGCACGCAAAATCATCA
ACCAGTGCACTCTCTGATGCTTACAGAAGACAACGCCCTTAACCGTCAGAAAATACATGAAGCTGCTGATAAGATTCATT
ATGCTCTGACAAGTGTTCCTGGAATCAGCTCGGGGAAAATTATTTTTTCTCTAAGTAAAAATTCTCAAGATTGTGAATTA
AAACAAGGTGAGCTGTGGTCCGTAGATTATGACGGAGGGAATTTGCGCCCTCTTACACAAGAAAACTCCTTGTCTATTAC
TCCAAGTTGGATGCATATAGGGAGCAAGAAGCCCTACCTATATGTTTCCTATAAATTTGGTATTCCGAAGATTTTCCTAG
GTTCTTTAGAGAACACGACTGGGAAAAAGATTCTTCATTTACAGGGGAATCAGTTTATGCCAGCTTTTTCTCCGAGGAAA
AAGCTCTTAGCTTTTATTTCGGATGCGTATGGGAATCCTGATTTATTTCTTCAGAGTTTTTCCCTGTCACAAGGAGCTAT
GGGGAAACCCCGTAGAATACTTAATGAAACTTTTGGAACACAAGGCAACCCTTCTTTTAGTCCTGACGGCTCTAAGTTAG
TTTTTGTTTCCAATAAAGATGGTAGACCACGTTTATACATCCTTCAAATCGATCCTGAAATGCTATCGCCTCGATTATTA
ACCAAAAAATATAGAAATAGCAGTTGCCCTTCATGGTCTCCTGATGGTAAAAAAATAGCCTTTTGCTCTGTAATTAAGGG
TGTTCGGCAGATTTGTGTATATGATCTGGCGACTGGGAAAGATTATCAACTAACAACAACCCCTGTAGACAAGGAAGGGC
CTTCCTGGGCCGTGGATAGTCAGCATCTTGTGTATAGTGCAGGGAACACAGGAGAGTCAGAACTCTATTTATTAAGTCTG
ATTACCCAAAAAACTAAGAAAATTGTTATAGGATTAGGGGAAAAACGTTTTCCTTCTTGGGGAGGTTTCCCTGATAACCA
ATAA

Upstream 100 bases:

>100_bases
CCCTTTAAAAAATTCTTAGATAAATACAAAATCTCGAAAAATATCGTTTTCCATATTAAACTGCTGAGTAATGAGGCTTA
ACATCGTAGGGGTTATTGGA

Downstream 100 bases:

>100_bases
AGAGAATCTTATGAAAAAGAAATACCTAAGCGTTTTGAGTTGTTTACTACTTGCTCTTTTCGCCCTACCTTCATGCTCTT
ATCCTTGTGGTGATTGGGAT

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MLRRMLVSAFLMFGIISLYAKELEVSVRSEISRLPIHVELKIGSNDASQQKYLRSLCTTFINDLALGDRLQPSLVQTGSS
SAPFNIAMVSHYPEITFTLARGTQNHQPVHSLMLTEDNALNRQKIHEAADKIHYALTSVPGISSGKIIFSLSKNSQDCEL
KQGELWSVDYDGGNLRPLTQENSLSITPSWMHIGSKKPYLYVSYKFGIPKIFLGSLENTTGKKILHLQGNQFMPAFSPRK
KLLAFISDAYGNPDLFLQSFSLSQGAMGKPRRILNETFGTQGNPSFSPDGSKLVFVSNKDGRPRLYILQIDPEMLSPRLL
TKKYRNSSCPSWSPDGKKIAFCSVIKGVRQICVYDLATGKDYQLTTTPVDKEGPSWAVDSQHLVYSAGNTGESELYLLSL
ITQKTKKIVIGLGEKRFPSWGGFPDNQ

Sequences:

>Translated_427_residues
MLRRMLVSAFLMFGIISLYAKELEVSVRSEISRLPIHVELKIGSNDASQQKYLRSLCTTFINDLALGDRLQPSLVQTGSS
SAPFNIAMVSHYPEITFTLARGTQNHQPVHSLMLTEDNALNRQKIHEAADKIHYALTSVPGISSGKIIFSLSKNSQDCEL
KQGELWSVDYDGGNLRPLTQENSLSITPSWMHIGSKKPYLYVSYKFGIPKIFLGSLENTTGKKILHLQGNQFMPAFSPRK
KLLAFISDAYGNPDLFLQSFSLSQGAMGKPRRILNETFGTQGNPSFSPDGSKLVFVSNKDGRPRLYILQIDPEMLSPRLL
TKKYRNSSCPSWSPDGKKIAFCSVIKGVRQICVYDLATGKDYQLTTTPVDKEGPSWAVDSQHLVYSAGNTGESELYLLSL
ITQKTKKIVIGLGEKRFPSWGGFPDNQ
>Mature_427_residues
MLRRMLVSAFLMFGIISLYAKELEVSVRSEISRLPIHVELKIGSNDASQQKYLRSLCTTFINDLALGDRLQPSLVQTGSS
SAPFNIAMVSHYPEITFTLARGTQNHQPVHSLMLTEDNALNRQKIHEAADKIHYALTSVPGISSGKIIFSLSKNSQDCEL
KQGELWSVDYDGGNLRPLTQENSLSITPSWMHIGSKKPYLYVSYKFGIPKIFLGSLENTTGKKILHLQGNQFMPAFSPRK
KLLAFISDAYGNPDLFLQSFSLSQGAMGKPRRILNETFGTQGNPSFSPDGSKLVFVSNKDGRPRLYILQIDPEMLSPRLL
TKKYRNSSCPSWSPDGKKIAFCSVIKGVRQICVYDLATGKDYQLTTTPVDKEGPSWAVDSQHLVYSAGNTGESELYLLSL
ITQKTKKIVIGLGEKRFPSWGGFPDNQ

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=301, Percent_Identity=27.906976744186, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_CHLAB (Q5L4R3)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_220329.1
- ProteinModelPortal:   Q5L4R3
- SMR:   Q5L4R3
- GeneID:   3337943
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB944
- HOGENOM:   HBG415163
- OMA:   LKDNTES
- PhylomeDB:   Q5L4R3
- ProtClustDB:   PRK01029
- BioCyc:   CABO218497:CAB944-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR015943
- Gene3D:   G3DSA:2.130.10.10

Pfam domain/function: PF07676 PD40

EC number: NA

Molecular weight: Translated: 47512; Mature: 47512

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRMLVSAFLMFGIISLYAKELEVSVRSEISRLPIHVELKIGSNDASQQKYLRSLCTTF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHH
INDLALGDRLQPSLVQTGSSSAPFNIAMVSHYPEITFTLARGTQNHQPVHSLMLTEDNAL
HHHHHCCCCCCCHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCHHEEEEECCCCC
NRQKIHEAADKIHYALTSVPGISSGKIIFSLSKNSQDCELKQGELWSVDYDGGNLRPLTQ
CHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCC
ENSLSITPSWMHIGSKKPYLYVSYKFGIPKIFLGSLENTTGKKILHLQGNQFMPAFSPRK
CCCEEECCHHHCCCCCCCEEEEEEECCCCEEEECCCCCCCCCEEEEECCCCCCCCCCHHH
KLLAFISDAYGNPDLFLQSFSLSQGAMGKPRRILNETFGTQGNPSFSPDGSKLVFVSNKD
HHHHHHHHCCCCHHHEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCC
GRPRLYILQIDPEMLSPRLLTKKYRNSSCPSWSPDGKKIAFCSVIKGVRQICVYDLATGK
CCCEEEEEEECHHHHCCHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCC
DYQLTTTPVDKEGPSWAVDSQHLVYSAGNTGESELYLLSLITQKTKKIVIGLGEKRFPSW
CEEEEECCCCCCCCCEECCCCEEEEECCCCCCCCEEEHHHHHHCCCEEEEECCCCCCCCC
GGFPDNQ
CCCCCCC
>Mature Secondary Structure
MLRRMLVSAFLMFGIISLYAKELEVSVRSEISRLPIHVELKIGSNDASQQKYLRSLCTTF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHH
INDLALGDRLQPSLVQTGSSSAPFNIAMVSHYPEITFTLARGTQNHQPVHSLMLTEDNAL
HHHHHCCCCCCCHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCHHEEEEECCCCC
NRQKIHEAADKIHYALTSVPGISSGKIIFSLSKNSQDCELKQGELWSVDYDGGNLRPLTQ
CHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCC
ENSLSITPSWMHIGSKKPYLYVSYKFGIPKIFLGSLENTTGKKILHLQGNQFMPAFSPRK
CCCEEECCHHHCCCCCCCEEEEEEECCCCEEEECCCCCCCCCEEEEECCCCCCCCCCHHH
KLLAFISDAYGNPDLFLQSFSLSQGAMGKPRRILNETFGTQGNPSFSPDGSKLVFVSNKD
HHHHHHHHCCCCHHHEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCC
GRPRLYILQIDPEMLSPRLLTKKYRNSSCPSWSPDGKKIAFCSVIKGVRQICVYDLATGK
CCCEEEEEEECHHHHCCHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCC
DYQLTTTPVDKEGPSWAVDSQHLVYSAGNTGESELYLLSLITQKTKKIVIGLGEKRFPSW
CEEEEECCCCCCCCCEECCCCEEEEECCCCCCCCEEEHHHHHHCCCEEEEECCCCCCCCC
GGFPDNQ
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA