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The map label for this gene is eno

Identifier: 62185532

GI number: 62185532

Start: 1088083

End: 1089357

Strand: Direct

Name: eno

Synonym: CAB932

Alternate gene names: 62185532

Gene position: 1088083-1089357 (Clockwise)

Preceding gene: 62185531

Following gene: 62185533

Centisome position: 95.08

GC content: 42.12

Gene sequence:

>1275_bases
ATGCTAGAAGTTGTCATTTCCGATATCCAAGCTAGAGAAATTTTAGATTCCAGAGGATATCCCACACTATATGTTAAAGT
AATTACAAACACAGGCACGTTCGGAGAAGCTTGTGTGCCTTCGGGAGCCTCCACAGGAATTAAAGAAGCTTTAGAGCTTC
GCGATCAAGATACCTCTCGATACCAAGGGAAAGGTGTTTTACAAGCCTTAAAAAACGTGAAAGAAGTTCTGCTTCCTGTT
TTGCAAGGAGTCAGCATATTTGATCAAATCCTTATAGACTCTATTATGGTAGAAGCAGATGGCACACCAAATAAAGAGAA
ATTAGGAGCCAATGCTATCTTAGGGGTATCCTTAGCAGCTGCAAAAGCAGCAGCCGCAACTTTAGGACGATCTTTTTACC
GTTACGTGGGAGGATGTTTCGCTCACATTCTTCCCTGCCCTATGATGAATCTCATTAATGGGGGTATGCATGCAAACAAC
GGCCTGCAATTTCAAGAATTTATGATTCGCCCTATAGGCGCACATTCTCTACAAGAAGCTGTACGTATGGGTGCCGATGT
GTTCCATACATTGAAAAACCTTCTCAATGATAAACATCTCGCTACAGGAGTGGGAGATGAAGGCGGATTTGCTCCACAAT
TAAAATCTAACTCTGAAGCTTTAGACCTTCTTGTATTAGCTATTGAGAAAGCCGGTTTCCAGCCTGGCGAGGAGATCTCT
TTAGCTCTTGACTGTGCTGCATCTTCTTTCTACGATACAAAAACAGAAACTTACGCAGGGAAAAACTCTCAAGAACAAGT
CGGCATACTCGCCGATCTTTGTGATCGTTATCCTATAGACTCTATAGAGGATGGGCTTGCTGAAGAAGATTTTGACGGTT
GGGAATTGCTAACTGCAGAACTCGGAGAAAATATACAAATTGTCGGTGATGACCTCTTCGTCACCAATCCAGAGTTGATA
GCAGAAGGTATAAGCAAAGGCCTTGCTAACGCTGTGCTCATTAAACCTAATCAAATCGGCACATTAACAGAAACTTCAGA
AGCTATACAACTTGCCCATAGTCAAGGCTATACAACTATTCTTTCTCATAGATCCGGAGAAACTGAAGACACAACAATTG
CCGATCTTGCTGTGGCCTTCAATACAGGGCAAATTAAAACTGGATCCTTATCACGCTCGGAACGCATTGCCAAGTACAAT
AGGCTTATGGCAATAGAAGAAGAACTGGGGTCTGAAGGTTTATTTAAAGATTCTAACCCATTTTCTGGAGAATAG

Upstream 100 bases:

>100_bases
GCAACCTCATGGTGATCATATGATCCGTGTGTTCATATTGTGTTAAATTAGGTCTTTGGGAGCTAGTTTCCAACCCCTTG
ACAAAAGAGATTAAAGACTG

Downstream 100 bases:

>100_bases
CATCGGATCGATCGCTTTCTTGTGATTACTAGAAAGCGATTGTCTTTTTCAGCTTCTCTGTGATGAAATTTATGCTTCTA
TTCTTAAGAATCATTCTAAA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MLEVVISDIQAREILDSRGYPTLYVKVITNTGTFGEACVPSGASTGIKEALELRDQDTSRYQGKGVLQALKNVKEVLLPV
LQGVSIFDQILIDSIMVEADGTPNKEKLGANAILGVSLAAAKAAAATLGRSFYRYVGGCFAHILPCPMMNLINGGMHANN
GLQFQEFMIRPIGAHSLQEAVRMGADVFHTLKNLLNDKHLATGVGDEGGFAPQLKSNSEALDLLVLAIEKAGFQPGEEIS
LALDCAASSFYDTKTETYAGKNSQEQVGILADLCDRYPIDSIEDGLAEEDFDGWELLTAELGENIQIVGDDLFVTNPELI
AEGISKGLANAVLIKPNQIGTLTETSEAIQLAHSQGYTTILSHRSGETEDTTIADLAVAFNTGQIKTGSLSRSERIAKYN
RLMAIEEELGSEGLFKDSNPFSGE

Sequences:

>Translated_424_residues
MLEVVISDIQAREILDSRGYPTLYVKVITNTGTFGEACVPSGASTGIKEALELRDQDTSRYQGKGVLQALKNVKEVLLPV
LQGVSIFDQILIDSIMVEADGTPNKEKLGANAILGVSLAAAKAAAATLGRSFYRYVGGCFAHILPCPMMNLINGGMHANN
GLQFQEFMIRPIGAHSLQEAVRMGADVFHTLKNLLNDKHLATGVGDEGGFAPQLKSNSEALDLLVLAIEKAGFQPGEEIS
LALDCAASSFYDTKTETYAGKNSQEQVGILADLCDRYPIDSIEDGLAEEDFDGWELLTAELGENIQIVGDDLFVTNPELI
AEGISKGLANAVLIKPNQIGTLTETSEAIQLAHSQGYTTILSHRSGETEDTTIADLAVAFNTGQIKTGSLSRSERIAKYN
RLMAIEEELGSEGLFKDSNPFSGE
>Mature_424_residues
MLEVVISDIQAREILDSRGYPTLYVKVITNTGTFGEACVPSGASTGIKEALELRDQDTSRYQGKGVLQALKNVKEVLLPV
LQGVSIFDQILIDSIMVEADGTPNKEKLGANAILGVSLAAAKAAAATLGRSFYRYVGGCFAHILPCPMMNLINGGMHANN
GLQFQEFMIRPIGAHSLQEAVRMGADVFHTLKNLLNDKHLATGVGDEGGFAPQLKSNSEALDLLVLAIEKAGFQPGEEIS
LALDCAASSFYDTKTETYAGKNSQEQVGILADLCDRYPIDSIEDGLAEEDFDGWELLTAELGENIQIVGDDLFVTNPELI
AEGISKGLANAVLIKPNQIGTLTETSEAIQLAHSQGYTTILSHRSGETEDTTIADLAVAFNTGQIKTGSLSRSERIAKYN
RLMAIEEELGSEGLFKDSNPFSGE

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=51.8604651162791, Blast_Score=394, Evalue=1e-110,
Organism=Homo sapiens, GI301897477, Length=436, Percent_Identity=50.2293577981651, Blast_Score=386, Evalue=1e-107,
Organism=Homo sapiens, GI301897469, Length=436, Percent_Identity=50.2293577981651, Blast_Score=386, Evalue=1e-107,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=49.5348837209302, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI301897479, Length=434, Percent_Identity=46.0829493087558, Blast_Score=332, Evalue=5e-91,
Organism=Homo sapiens, GI169201331, Length=350, Percent_Identity=28, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI169201757, Length=350, Percent_Identity=28, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI239744207, Length=350, Percent_Identity=28, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI310129182, Length=139, Percent_Identity=33.8129496402878, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI310110045, Length=139, Percent_Identity=33.8129496402878, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI310120572, Length=139, Percent_Identity=33.8129496402878, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789141, Length=428, Percent_Identity=52.803738317757, Blast_Score=419, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=48.491879350348, Blast_Score=373, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=48.491879350348, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=44.7916666666667, Blast_Score=157, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6321693, Length=440, Percent_Identity=47.7272727272727, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6323985, Length=429, Percent_Identity=45.4545454545455, Blast_Score=342, Evalue=7e-95,
Organism=Saccharomyces cerevisiae, GI6324974, Length=429, Percent_Identity=45.4545454545455, Blast_Score=341, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6324969, Length=429, Percent_Identity=45.4545454545455, Blast_Score=341, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6321968, Length=440, Percent_Identity=46.3636363636364, Blast_Score=337, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24580918, Length=427, Percent_Identity=49.8829039812646, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580916, Length=427, Percent_Identity=49.8829039812646, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580920, Length=427, Percent_Identity=49.8829039812646, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580914, Length=427, Percent_Identity=49.8829039812646, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI281360527, Length=426, Percent_Identity=49.7652582159624, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI17137654, Length=426, Percent_Identity=49.7652582159624, Blast_Score=353, Evalue=1e-97,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_CHLAB (Q5L4S5)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_220317.1
- HSSP:   Q97QS2
- ProteinModelPortal:   Q5L4S5
- SMR:   Q5L4S5
- GeneID:   3338035
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB932
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   CABO218497:CAB932-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45464; Mature: 45464

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 207-207 ACT_SITE 335-335 BINDING 157-157 BINDING 166-166 BINDING 283-283 BINDING 310-310 BINDING 335-335 BINDING 386-386

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEVVISDIQAREILDSRGYPTLYVKVITNTGTFGEACVPSGASTGIKEALELRDQDTSR
CCEEEEHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCHH
YQGKGVLQALKNVKEVLLPVLQGVSIFDQILIDSIMVEADGTPNKEKLGANAILGVSLAA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHCCCHHHHHHHHH
AKAAAATLGRSFYRYVGGCFAHILPCPMMNLINGGMHANNGLQFQEFMIRPIGAHSLQEA
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHHHH
VRMGADVFHTLKNLLNDKHLATGVGDEGGFAPQLKSNSEALDLLVLAIEKAGFQPGEEIS
HHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
LALDCAASSFYDTKTETYAGKNSQEQVGILADLCDRYPIDSIEDGLAEEDFDGWELLTAE
EEEEHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHH
LGENIQIVGDDLFVTNPELIAEGISKGLANAVLIKPNQIGTLTETSEAIQLAHSQGYTTI
HCCCEEEEECCEEECCHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEE
LSHRSGETEDTTIADLAVAFNTGQIKTGSLSRSERIAKYNRLMAIEEELGSEGLFKDSNP
EECCCCCCCCCHHHHEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FSGE
CCCC
>Mature Secondary Structure
MLEVVISDIQAREILDSRGYPTLYVKVITNTGTFGEACVPSGASTGIKEALELRDQDTSR
CCEEEEHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCHH
YQGKGVLQALKNVKEVLLPVLQGVSIFDQILIDSIMVEADGTPNKEKLGANAILGVSLAA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHCCCHHHHHHHHH
AKAAAATLGRSFYRYVGGCFAHILPCPMMNLINGGMHANNGLQFQEFMIRPIGAHSLQEA
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHHHH
VRMGADVFHTLKNLLNDKHLATGVGDEGGFAPQLKSNSEALDLLVLAIEKAGFQPGEEIS
HHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
LALDCAASSFYDTKTETYAGKNSQEQVGILADLCDRYPIDSIEDGLAEEDFDGWELLTAE
EEEEHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHH
LGENIQIVGDDLFVTNPELIAEGISKGLANAVLIKPNQIGTLTETSEAIQLAHSQGYTTI
HCCCEEEEECCEEECCHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEE
LSHRSGETEDTTIADLAVAFNTGQIKTGSLSRSERIAKYNRLMAIEEELGSEGLFKDSNP
EECCCCCCCCCHHHHEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FSGE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA