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The map label for this gene is birA [H]

Identifier: 62185471

GI number: 62185471

Start: 1014102

End: 1014674

Strand: Direct

Name: birA [H]

Synonym: CAB869

Alternate gene names: 62185471

Gene position: 1014102-1014674 (Clockwise)

Preceding gene: 62185469

Following gene: 62185474

Centisome position: 88.62

GC content: 39.79

Gene sequence:

>573_bases
ATGAAAGTTATTTATTATGAAATTGCAGAAACTCCATCTACAAATGCAACAGCAAAACAATTCATGCATGTGTGGAATCC
TTTTGCTCTTACTGTTGTGTCCACTCAAAAACAAACCGACGGAAAGGGGAAATTTAATAAGTCCTGGGTATCTTCTAATA
AAGACCTTACCCTTTCCTTCTGCTTTTTCATTACTGAACTTGATATTGATGTTAGCTTATTATTTCGTTTAGGGACAGAA
GCGGTACTCGAATTAATTCAGGATTTAGGCATCACAAACGGAACCATAAAATGGCCTAATGATGTTTTGGTACATGGTGA
AAAGCTCTGTGGTGTTCTTAGCGAAACTCTACCATCCCAAGGGTACTTGGGTATCGTTTTAGGAATAGGGATCAATGGCA
ATAGTTCCCAGGAAGACCTAGCCTCTATTGAGCAACCTGCCACCTCTCTCTCTATACTTTTACAGCATCCTATAGACCTT
GAAGAGATCCGTCATCGGCTTGTGGAACATGTGCGGAAGTGCATCTTGCAAAGATTCTCAAAAATCTTAGCGAGAGAAAT
GAATCACGGGTAA

Upstream 100 bases:

>100_bases
ATTAATAAAATAATGACAACAAATACCCAAGAATTGACATAGCTGAAATATCTAGTGTTTCTCATAATGCTCTCTATAGC
TTAAGAGTGGGATACTTCCA

Downstream 100 bases:

>100_bases
ATCTAAAAAACGAAATACTCTCGAAGCTACCCATAATGACGGTCGGGATCTGTGTTCCAAATATTTCTCCACGGCTTGTT
CATAAGCCGCCGTCTCAAAA

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MKVIYYEIAETPSTNATAKQFMHVWNPFALTVVSTQKQTDGKGKFNKSWVSSNKDLTLSFCFFITELDIDVSLLFRLGTE
AVLELIQDLGITNGTIKWPNDVLVHGEKLCGVLSETLPSQGYLGIVLGIGINGNSSQEDLASIEQPATSLSILLQHPIDL
EEIRHRLVEHVRKCILQRFSKILAREMNHG

Sequences:

>Translated_190_residues
MKVIYYEIAETPSTNATAKQFMHVWNPFALTVVSTQKQTDGKGKFNKSWVSSNKDLTLSFCFFITELDIDVSLLFRLGTE
AVLELIQDLGITNGTIKWPNDVLVHGEKLCGVLSETLPSQGYLGIVLGIGINGNSSQEDLASIEQPATSLSILLQHPIDL
EEIRHRLVEHVRKCILQRFSKILAREMNHG
>Mature_190_residues
MKVIYYEIAETPSTNATAKQFMHVWNPFALTVVSTQKQTDGKGKFNKSWVSSNKDLTLSFCFFITELDIDVSLLFRLGTE
AVLELIQDLGITNGTIKWPNDVLVHGEKLCGVLSETLPSQGYLGIVLGIGINGNSSQEDLASIEQPATSLSILLQHPIDL
EEIRHRLVEHVRKCILQRFSKILAREMNHG

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 21225; Mature: 21225

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIYYEIAETPSTNATAKQFMHVWNPFALTVVSTQKQTDGKGKFNKSWVSSNKDLTLSF
CEEEEEEECCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHCCCCCEEEEH
CFFITELDIDVSLLFRLGTEAVLELIQDLGITNGTIKWPNDVLVHGEKLCGVLSETLPSQ
EEEHHHCCCHHHHHHHHCHHHHHHHHHHCCCCCCEEECCCHHEECHHHHHHHHHHHCCCC
GYLGIVLGIGINGNSSQEDLASIEQPATSLSILLQHPIDLEEIRHRLVEHVRKCILQRFS
CCEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KILAREMNHG
HHHHHHHCCC
>Mature Secondary Structure
MKVIYYEIAETPSTNATAKQFMHVWNPFALTVVSTQKQTDGKGKFNKSWVSSNKDLTLSF
CEEEEEEECCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHCCCCCEEEEH
CFFITELDIDVSLLFRLGTEAVLELIQDLGITNGTIKWPNDVLVHGEKLCGVLSETLPSQ
EEEHHHCCCHHHHHHHHCHHHHHHHHHHCCCCCCEEECCCHHEECHHHHHHHHHHHCCCC
GYLGIVLGIGINGNSSQEDLASIEQPATSLSILLQHPIDLEEIRHRLVEHVRKCILQRFS
CCEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KILAREMNHG
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]