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The map label for this gene is cadA [H]

Identifier: 62185469

GI number: 62185469

Start: 1010950

End: 1012923

Strand: Direct

Name: cadA [H]

Synonym: CAB867

Alternate gene names: 62185469

Gene position: 1010950-1012923 (Clockwise)

Preceding gene: 62185468

Following gene: 62185471

Centisome position: 88.34

GC content: 41.84

Gene sequence:

>1974_bases
ATGTTTTCACAACTATTCTCTTCTCCATTTTCACCAGAAAAGCTAAATAATTTTTTTGAGTCGGGGATGACCGAAGACAA
TAGTCCGATGTTATCTCAAGGGAGCCGGTTACTCAACCGTAATTTATCTTTAAAATCGGCGTGTGTTTCCTTAGGGATGT
ACTTGTGTGCATTAGTGTTCTATTGGTTTCACGTTACAGAGATTTCCAATCTGTTTGTCGTTTTTACATTCTTTCTTGCT
GGGACTCCAGCTTTGATAAAATCGTTAGATGATATTCGCAATAAGACTGTAAATATTGACATCTTAATGACGTCAGCAGC
GTTCGGATCGATTTTTATTGGTGGAGCTTTAGAGGGATCTTTACTTCTTGTTTTGTTTGCTATTTCTGAAGCTTTAGGGC
AAATGGTATCCGGGAAAGCCAAGGGAACATTAGCCTCTTTGAAACATCTAGCTCCCACAATAGCCTGGGTAGTCCAAGAA
GATGGGAATTTAGAGAAAATTCCCGTACGTCTTGTAGAAGTTGGTCATATCATTCGAGTAAAAAGTGGCGAGATTGTTCC
TTTAGATGGAGAAATTGTTCACGGATCTTCCTCGATCAATCTTATGCATCTTACCGGGGAGAAAATTCCTAAATCTTGTC
AGGTTGGCTCTATAGTTCCTGCAGGGGCGCACAATCTCGAGGGAAGTTTTGATCTTAAGGTTTTGAAAACAGGTGCGGAC
TCTACCATCTCTCACATTATCAATTTAGTTATTCAAGCGCAGAAATCGAAACCACGTTTACAACAACGTCTAGACAAGTA
TTCTTCAGTTTATGCGTTGACGATTTTTGCTATCTCTACCTCTATAGCGGTTTTAGTCCCTCTATTTACCTCGATTCCTT
TCTTAGGGCCTAATAGTGCGTTTTATCGTGCTTTAGCTTTTCTCATAGCAGCATCTCCCTGCGCATTAATTATAGCTATT
CCTATTGCCTATCTCAGCGCTGTAAATGCTTGTGCAAAGCATGGTGTTCTTCTTAAAGGTGGTGTCGTTTTAGACCGTTT
AGCTTCCTGTAATTCTATTGTTATGGATAAAACAGGGACCCTCACAACCGGAGAGCTTACCTGTATTGGTTGTGATACGT
TCGGTAATGAACATCCTGATTTCTTCCCTTCGATTTTAGCTTTAGAACAATCTTCCATGCATCCTATAGCTCAAGCAATT
GTTTCCTACCTCATGAAAAATAATGTTTCCTCACGTCCTGCAGAAGAGTACTGCATGATTCCAGGACAAGGTGTACGAGG
GCTTTTTGAAGGCAAGGAAGCATTCGTAGGGAGAGTAGATACTGCCTTACAAAAAATTCCCCAAGATTACGTTCAAGAAT
TAGAAGAGCGTATACACACTGCACGACAACGCGGAGAAATCTGCTCTCTTGCTTATTTGCAAGATTGTTATGCTCTCTTT
TATTTTAAAGACACACCACGTCCTGATGCCGAAAAAATTGTCAAGGAGCTTCGAAACGACGGGTATACGATTAGCATGTT
GACAGGAGACCATCAAATTAGTGCAGAAAATACTGCCAAGCTTTTGGGGATCTCCGAGGTATTTGCAAATTTATCTCCCG
ATGACAAATTAGACAAAGTACGTGAACTTGCGAAGCAACGTCATATTCTCATGGTTGGGGATGGGATCAATGATGCTCCT
GCTCTTGCCCAAGCCACGGTAGGTGTCGCTATGGGAGAAGCAGGCAGTGCGACAGCTATAGAAGCTGCTGATATAGTTCT
CCTTCATGATGCGATTTCCCTACTACCCTGGATTATAAGGAAAGCTAAGCAAACACGAAGGATCGTGACCCAAAACTTGG
GATTAGCTCTTGCGATTATTCTTCTTGTTTCTTGGCCAGCATCTTTGGGGATTATTCCCTTATGGTTAGCCGTGATCCTT
CACGAGGGGAGCACTATCGTTGTAGGGCTCAATGCTTTGCGCTTGCTGAAATAG

Upstream 100 bases:

>100_bases
TGATATGATTACAGAAGTTGTATGGGTTTTAGGTATTTTTATTACCTCGATAAGTATCGTTTGCACTTGTGTAAAATTTT
TATAGATCCTAGGTATCATA

Downstream 100 bases:

>100_bases
TTCTTAGTATTTTGAGAATCTACGGCTATAGATACTCTGTAAAATACCCAATGAAGCCATAGTCGAAATGACTGAAGATC
CTCCATAGGAAACTAAAACG

Product: putative cation transport related membrane protein

Products: NA

Alternate protein names: Cadmium efflux ATPase [H]

Number of amino acids: Translated: 657; Mature: 657

Protein sequence:

>657_residues
MFSQLFSSPFSPEKLNNFFESGMTEDNSPMLSQGSRLLNRNLSLKSACVSLGMYLCALVFYWFHVTEISNLFVVFTFFLA
GTPALIKSLDDIRNKTVNIDILMTSAAFGSIFIGGALEGSLLLVLFAISEALGQMVSGKAKGTLASLKHLAPTIAWVVQE
DGNLEKIPVRLVEVGHIIRVKSGEIVPLDGEIVHGSSSINLMHLTGEKIPKSCQVGSIVPAGAHNLEGSFDLKVLKTGAD
STISHIINLVIQAQKSKPRLQQRLDKYSSVYALTIFAISTSIAVLVPLFTSIPFLGPNSAFYRALAFLIAASPCALIIAI
PIAYLSAVNACAKHGVLLKGGVVLDRLASCNSIVMDKTGTLTTGELTCIGCDTFGNEHPDFFPSILALEQSSMHPIAQAI
VSYLMKNNVSSRPAEEYCMIPGQGVRGLFEGKEAFVGRVDTALQKIPQDYVQELEERIHTARQRGEICSLAYLQDCYALF
YFKDTPRPDAEKIVKELRNDGYTISMLTGDHQISAENTAKLLGISEVFANLSPDDKLDKVRELAKQRHILMVGDGINDAP
ALAQATVGVAMGEAGSATAIEAADIVLLHDAISLLPWIIRKAKQTRRIVTQNLGLALAIILLVSWPASLGIIPLWLAVIL
HEGSTIVVGLNALRLLK

Sequences:

>Translated_657_residues
MFSQLFSSPFSPEKLNNFFESGMTEDNSPMLSQGSRLLNRNLSLKSACVSLGMYLCALVFYWFHVTEISNLFVVFTFFLA
GTPALIKSLDDIRNKTVNIDILMTSAAFGSIFIGGALEGSLLLVLFAISEALGQMVSGKAKGTLASLKHLAPTIAWVVQE
DGNLEKIPVRLVEVGHIIRVKSGEIVPLDGEIVHGSSSINLMHLTGEKIPKSCQVGSIVPAGAHNLEGSFDLKVLKTGAD
STISHIINLVIQAQKSKPRLQQRLDKYSSVYALTIFAISTSIAVLVPLFTSIPFLGPNSAFYRALAFLIAASPCALIIAI
PIAYLSAVNACAKHGVLLKGGVVLDRLASCNSIVMDKTGTLTTGELTCIGCDTFGNEHPDFFPSILALEQSSMHPIAQAI
VSYLMKNNVSSRPAEEYCMIPGQGVRGLFEGKEAFVGRVDTALQKIPQDYVQELEERIHTARQRGEICSLAYLQDCYALF
YFKDTPRPDAEKIVKELRNDGYTISMLTGDHQISAENTAKLLGISEVFANLSPDDKLDKVRELAKQRHILMVGDGINDAP
ALAQATVGVAMGEAGSATAIEAADIVLLHDAISLLPWIIRKAKQTRRIVTQNLGLALAIILLVSWPASLGIIPLWLAVIL
HEGSTIVVGLNALRLLK
>Mature_657_residues
MFSQLFSSPFSPEKLNNFFESGMTEDNSPMLSQGSRLLNRNLSLKSACVSLGMYLCALVFYWFHVTEISNLFVVFTFFLA
GTPALIKSLDDIRNKTVNIDILMTSAAFGSIFIGGALEGSLLLVLFAISEALGQMVSGKAKGTLASLKHLAPTIAWVVQE
DGNLEKIPVRLVEVGHIIRVKSGEIVPLDGEIVHGSSSINLMHLTGEKIPKSCQVGSIVPAGAHNLEGSFDLKVLKTGAD
STISHIINLVIQAQKSKPRLQQRLDKYSSVYALTIFAISTSIAVLVPLFTSIPFLGPNSAFYRALAFLIAASPCALIIAI
PIAYLSAVNACAKHGVLLKGGVVLDRLASCNSIVMDKTGTLTTGELTCIGCDTFGNEHPDFFPSILALEQSSMHPIAQAI
VSYLMKNNVSSRPAEEYCMIPGQGVRGLFEGKEAFVGRVDTALQKIPQDYVQELEERIHTARQRGEICSLAYLQDCYALF
YFKDTPRPDAEKIVKELRNDGYTISMLTGDHQISAENTAKLLGISEVFANLSPDDKLDKVRELAKQRHILMVGDGINDAP
ALAQATVGVAMGEAGSATAIEAADIVLLHDAISLLPWIIRKAKQTRRIVTQNLGLALAIILLVSWPASLGIIPLWLAVIL
HEGSTIVVGLNALRLLK

Specific function: This electroneutral antiporter ejects one cadmium ion while accumulating two protons by an energy-dependent efflux mechanism [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 HMA domains [H]

Homologues:

Organism=Homo sapiens, GI115529486, Length=674, Percent_Identity=28.6350148367953, Blast_Score=203, Evalue=4e-52,
Organism=Homo sapiens, GI55743071, Length=648, Percent_Identity=28.7037037037037, Blast_Score=202, Evalue=8e-52,
Organism=Homo sapiens, GI55743073, Length=548, Percent_Identity=29.7445255474453, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI48762687, Length=635, Percent_Identity=23.4645669291339, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI48762685, Length=635, Percent_Identity=23.4645669291339, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI48762689, Length=635, Percent_Identity=23.4645669291339, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI48762691, Length=635, Percent_Identity=23.4645669291339, Blast_Score=93, Evalue=7e-19,
Organism=Escherichia coli, GI1789879, Length=563, Percent_Identity=33.392539964476, Blast_Score=257, Evalue=2e-69,
Organism=Escherichia coli, GI1786691, Length=542, Percent_Identity=33.5793357933579, Blast_Score=222, Evalue=6e-59,
Organism=Escherichia coli, GI1786914, Length=549, Percent_Identity=26.4116575591985, Blast_Score=116, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17556548, Length=582, Percent_Identity=30.5841924398625, Blast_Score=162, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI32566766, Length=191, Percent_Identity=32.4607329842932, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17557486, Length=197, Percent_Identity=30.9644670050761, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17557770, Length=191, Percent_Identity=32.4607329842932, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI193203356, Length=198, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI193203354, Length=198, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6319772, Length=557, Percent_Identity=28.5457809694794, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6320475, Length=548, Percent_Identity=29.3795620437956, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6325221, Length=577, Percent_Identity=24.4367417677643, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6321271, Length=695, Percent_Identity=22.589928057554, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI221329854, Length=404, Percent_Identity=28.2178217821782, Blast_Score=123, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24668704, Length=578, Percent_Identity=23.356401384083, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI281366617, Length=578, Percent_Identity=23.356401384083, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24668708, Length=578, Percent_Identity=23.356401384083, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24668696, Length=578, Percent_Identity=23.356401384083, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI161085803, Length=580, Percent_Identity=22.9310344827586, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR001366
- InterPro:   IPR006404
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: =3.6.3.3 [H]

Molecular weight: Translated: 71029; Mature: 71029

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00154 ATPASE_E1_E2 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSQLFSSPFSPEKLNNFFESGMTEDNSPMLSQGSRLLNRNLSLKSACVSLGMYLCALVF
CCHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YWFHVTEISNLFVVFTFFLAGTPALIKSLDDIRNKTVNIDILMTSAAFGSIFIGGALEGS
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEEEECCCCCCEEECCCCCHH
LLLVLFAISEALGQMVSGKAKGTLASLKHLAPTIAWVVQEDGNLEKIPVRLVEVGHIIRV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHEECCCEEEE
KSGEIVPLDGEIVHGSSSINLMHLTGEKIPKSCQVGSIVPAGAHNLEGSFDLKVLKTGAD
CCCCEEECCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
STISHIINLVIQAQKSKPRLQQRLDKYSSVYALTIFAISTSIAVLVPLFTSIPFLGPNSA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
FYRALAFLIAASPCALIIAIPIAYLSAVNACAKHGVLLKGGVVLDRLASCNSIVMDKTGT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHCCCCEEECCCCC
LTTGELTCIGCDTFGNEHPDFFPSILALEQSSMHPIAQAIVSYLMKNNVSSRPAEEYCMI
EEECCEEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHEECC
PGQGVRGLFEGKEAFVGRVDTALQKIPQDYVQELEERIHTARQRGEICSLAYLQDCYALF
CCCCCCHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEE
YFKDTPRPDAEKIVKELRNDGYTISMLTGDHQISAENTAKLLGISEVFANLSPDDKLDKV
EECCCCCCCHHHHHHHHHCCCEEEEEEECCCEECCCHHHHHHHHHHHHHCCCCCHHHHHH
RELAKQRHILMVGDGINDAPALAQATVGVAMGEAGSATAIEAADIVLLHDAISLLPWIIR
HHHHHCCCEEEEECCCCCCHHHHHHHHHHEECCCCCCCEEHHHHHHHHHHHHHHHHHHHH
KAKQTRRIVTQNLGLALAIILLVSWPASLGIIPLWLAVILHEGSTIVVGLNALRLLK
HHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHCCCCEEEEECHHHHHCC
>Mature Secondary Structure
MFSQLFSSPFSPEKLNNFFESGMTEDNSPMLSQGSRLLNRNLSLKSACVSLGMYLCALVF
CCHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YWFHVTEISNLFVVFTFFLAGTPALIKSLDDIRNKTVNIDILMTSAAFGSIFIGGALEGS
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEEEECCCCCCEEECCCCCHH
LLLVLFAISEALGQMVSGKAKGTLASLKHLAPTIAWVVQEDGNLEKIPVRLVEVGHIIRV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHEECCCEEEE
KSGEIVPLDGEIVHGSSSINLMHLTGEKIPKSCQVGSIVPAGAHNLEGSFDLKVLKTGAD
CCCCEEECCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
STISHIINLVIQAQKSKPRLQQRLDKYSSVYALTIFAISTSIAVLVPLFTSIPFLGPNSA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
FYRALAFLIAASPCALIIAIPIAYLSAVNACAKHGVLLKGGVVLDRLASCNSIVMDKTGT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHCCCCEEECCCCC
LTTGELTCIGCDTFGNEHPDFFPSILALEQSSMHPIAQAIVSYLMKNNVSSRPAEEYCMI
EEECCEEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHEECC
PGQGVRGLFEGKEAFVGRVDTALQKIPQDYVQELEERIHTARQRGEICSLAYLQDCYALF
CCCCCCHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEE
YFKDTPRPDAEKIVKELRNDGYTISMLTGDHQISAENTAKLLGISEVFANLSPDDKLDKV
EECCCCCCCHHHHHHHHHCCCEEEEEEECCCEECCCHHHHHHHHHHHHHCCCCCHHHHHH
RELAKQRHILMVGDGINDAPALAQATVGVAMGEAGSATAIEAADIVLLHDAISLLPWIIR
HHHHHCCCEEEEECCCCCCHHHHHHHHHHEECCCCCCCEEHHHHHHHHHHHHHHHHHHHH
KAKQTRRIVTQNLGLALAIILLVSWPASLGIIPLWLAVILHEGSTIVVGLNALRLLK
HHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHCCCCEEEEECHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11302791 [H]