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The map label for this gene is cofH [H]

Identifier: 62185426

GI number: 62185426

Start: 952890

End: 953951

Strand: Direct

Name: cofH [H]

Synonym: CAB822

Alternate gene names: 62185426

Gene position: 952890-953951 (Clockwise)

Preceding gene: 62185421

Following gene: 62185429

Centisome position: 83.27

GC content: 44.07

Gene sequence:

>1062_bases
ATGAACTTATTTACTAGAATTTCCTTTGACGAGGGATTGGAGTTATTTAGAACAAGTCCTTTAGAAAAACTACAGGAGGC
TGCGAATATTTTACGCAAACAGCGCCATCCTGGTAATAAAGTCACGTATGTCCTAGACGCCAACCCGAACTACACCAACA
TTTGTAAAATTGATTGTACCTTCTGCGCGTTTTATAGAAAGCCTCACGCTTCTGACGCGTATCTCCTTTCTTTTGACAAA
TTTCGTTCTTTAATGCAACGATATATATCTATGGGAGTTAAAACTGTGCTTCTTCAAGGAGGGGTCCATCCGCAACTAGG
GATAGACTATCTGGAAGAATTCGTACGGATCACGGTGCAAGAATTCCCCTCGATCCACCCCCATTTCTTCTCTGCAGTAG
AAATTTCACATGCAGCCTCTGTATCCGGAATCAGTAACGAAGAGGCTTTAATGAGACTGTGGAATGCAGGGCAGAGGACG
ATTCCTGGAGGGGGTGCTGAAATTTTTTCCGAACGTGTGCGCAAGATTATATCGCCAAAAAAAATGGGGCCCGATGGGTG
GATTAATTTCCACAAGCTTGCCCATCGTTTAGGGTTCTACTCTACAGCAACAATGATGTTTGGGCATATTGAAAACGCTC
ATGATATTCTCTTGCATCTGCAAGCTCTCAGGGACGCTCAGGACGAAACTCCTGGGTTTTATAGCTTCATTCCTTGGAGC
TATAAGTCTGGAAATACCGCTTTAGGACGCAAGGTCCCTAATCAAGCCCCACCTGAGATGTATTATCGCATTCTCGCACT
GGCTCGAATCTTCTTAGACAACTTTGAGCATATCGCGGCTTCATGGTTTGGAGAAGGTAAAGAACACGGAGCTCGTGGAT
TACATTATGGGGCCGATGATTTTGGAGGAACGATTATTGATGAGAGCGTGCATAAATGTGCGGGATGGACTTTAAAGAGT
TCTGAAGAGGAAACTCGGACGATGATCGCTTCAGAAGGATTTATCCCTGTGGAGAGGAATACTTTCTATGAACCTATAGA
AACGCCTTCTTATCAACCATGA

Upstream 100 bases:

>100_bases
GAAGTATCCATAATAACAACTTAGGGTGTAGGGCTTGACAAAGAAATAATGAATAGAAGATAAAGTTTGCCCCCGCACTA
CATTATCGTCAATCGTTTTT

Downstream 100 bases:

>100_bases
AGAAAATAATCCTCTGCTATCTTTGCTGCTATCGTTTCTGCTGTTAATCCCAGGGTAGGCAATTCTCGAAATATAGGGTA
ACGTTTAAACCAGGTCCTTT

Product: hypothetical protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MNLFTRISFDEGLELFRTSPLEKLQEAANILRKQRHPGNKVTYVLDANPNYTNICKIDCTFCAFYRKPHASDAYLLSFDK
FRSLMQRYISMGVKTVLLQGGVHPQLGIDYLEEFVRITVQEFPSIHPHFFSAVEISHAASVSGISNEEALMRLWNAGQRT
IPGGGAEIFSERVRKIISPKKMGPDGWINFHKLAHRLGFYSTATMMFGHIENAHDILLHLQALRDAQDETPGFYSFIPWS
YKSGNTALGRKVPNQAPPEMYYRILALARIFLDNFEHIAASWFGEGKEHGARGLHYGADDFGGTIIDESVHKCAGWTLKS
SEEETRTMIASEGFIPVERNTFYEPIETPSYQP

Sequences:

>Translated_353_residues
MNLFTRISFDEGLELFRTSPLEKLQEAANILRKQRHPGNKVTYVLDANPNYTNICKIDCTFCAFYRKPHASDAYLLSFDK
FRSLMQRYISMGVKTVLLQGGVHPQLGIDYLEEFVRITVQEFPSIHPHFFSAVEISHAASVSGISNEEALMRLWNAGQRT
IPGGGAEIFSERVRKIISPKKMGPDGWINFHKLAHRLGFYSTATMMFGHIENAHDILLHLQALRDAQDETPGFYSFIPWS
YKSGNTALGRKVPNQAPPEMYYRILALARIFLDNFEHIAASWFGEGKEHGARGLHYGADDFGGTIIDESVHKCAGWTLKS
SEEETRTMIASEGFIPVERNTFYEPIETPSYQP
>Mature_353_residues
MNLFTRISFDEGLELFRTSPLEKLQEAANILRKQRHPGNKVTYVLDANPNYTNICKIDCTFCAFYRKPHASDAYLLSFDK
FRSLMQRYISMGVKTVLLQGGVHPQLGIDYLEEFVRITVQEFPSIHPHFFSAVEISHAASVSGISNEEALMRLWNAGQRT
IPGGGAEIFSERVRKIISPKKMGPDGWINFHKLAHRLGFYSTATMMFGHIENAHDILLHLQALRDAQDETPGFYSFIPWS
YKSGNTALGRKVPNQAPPEMYYRILALARIFLDNFEHIAASWFGEGKEHGARGLHYGADDFGGTIIDESVHKCAGWTLKS
SEEETRTMIASEGFIPVERNTFYEPIETPSYQP

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 40033; Mature: 40033

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLFTRISFDEGLELFRTSPLEKLQEAANILRKQRHPGNKVTYVLDANPNYTNICKIDCT
CCCEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEHH
FCAFYRKPHASDAYLLSFDKFRSLMQRYISMGVKTVLLQGGVHPQLGIDYLEEFVRITVQ
HHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
EFPSIHPHFFSAVEISHAASVSGISNEEALMRLWNAGQRTIPGGGAEIFSERVRKIISPK
HCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
KMGPDGWINFHKLAHRLGFYSTATMMFGHIENAHDILLHLQALRDAQDETPGFYSFIPWS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEECCCC
YKSGNTALGRKVPNQAPPEMYYRILALARIFLDNFEHIAASWFGEGKEHGARGLHYGADD
CCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHH
FGGTIIDESVHKCAGWTLKSSEEETRTMIASEGFIPVERNTFYEPIETPSYQP
HCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNLFTRISFDEGLELFRTSPLEKLQEAANILRKQRHPGNKVTYVLDANPNYTNICKIDCT
CCCEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEHH
FCAFYRKPHASDAYLLSFDKFRSLMQRYISMGVKTVLLQGGVHPQLGIDYLEEFVRITVQ
HHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
EFPSIHPHFFSAVEISHAASVSGISNEEALMRLWNAGQRTIPGGGAEIFSERVRKIISPK
HCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
KMGPDGWINFHKLAHRLGFYSTATMMFGHIENAHDILLHLQALRDAQDETPGFYSFIPWS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEECCCC
YKSGNTALGRKVPNQAPPEMYYRILALARIFLDNFEHIAASWFGEGKEHGARGLHYGADD
CCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHH
FGGTIIDESVHKCAGWTLKSSEEETRTMIASEGFIPVERNTFYEPIETPSYQP
HCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA