Click here to switch to the map view.

The map label for this gene is ppa [H]

Identifier: 62185421

GI number: 62185421

Start: 947666

End: 948316

Strand: Direct

Name: ppa [H]

Synonym: CAB816

Alternate gene names: 62185421

Gene position: 947666-948316 (Clockwise)

Preceding gene: 62185419

Following gene: 62185426

Centisome position: 82.81

GC content: 36.56

Gene sequence:

>651_bases
ATGTCTGAAAAACAATCTTTATCGATCATGCATCCTTGGCACGGTCCTATATTAACTCAAGATAACTATGAATCTCTATG
TTGTTATATAGAAATTACGCCGCAGGATTCTGTAAAGTTTGAATTAGACAAAACTACAGGTTTATTGAAAGTAGATCGCC
CTCAGAAATTTTCTAATTTCTGCCCTTGTTTATATGGATTATTACCTAGAACGTATTGTGGTGAACTTTCTGGGCAATAT
AGCGGAGAACAAAGTTTGAAAGCCGATATCCGTGGTGACGATGATCCCTTAGATGTTTGCGTGCTTACAGAAAAAAATAT
CACTCACGGCAACATCTTGCTTCAAGCACGTCCTATTGGTGGTCTGCGCATTATTGACTCTGGAGAAGCCGACGATAAAA
TTATCGCTGTTCTTGAAGACGATTTAGTCTTTTCAGAGATAAAAGATATCTCTGACTGTCCTTGTACTGTTCTTGATATG
ATTCAGCATTATTTTCTAACGTATAAAGCTACCCCAGAACACTTGATCAATGCAAAACCAGCAAAAATTGAAATTGTAGG
GATTTACGGGAAGAAAGAAGCACAAAAGGTCATTCAACTAGCACACGAAGACTATTTGAATAAATTTGTTAGAGAGAAAA
CAACTATATAA

Upstream 100 bases:

>100_bases
TAACTAAACCCTCTCCCTTCTCTTAAAATTCCTCTCGTGTTACTCTAGATTACGCAGAAAAACTCGTGAATAAAAAAAAA
GAGTATAATGAGAGTCGCTT

Downstream 100 bases:

>100_bases
CTCATGTTTCTGGAGAGGAGGCTCCTCTCCTTACATTCTTCAACTTAGAAAGAGAAGAGATAGCTGTTGATAAACTTATC
TGCTTCTACAGCAATCTCTT

Product: inorganic pyrophosphatase

Products: NA

Alternate protein names: Pyrophosphate phospho-hydrolase; PPase [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MSEKQSLSIMHPWHGPILTQDNYESLCCYIEITPQDSVKFELDKTTGLLKVDRPQKFSNFCPCLYGLLPRTYCGELSGQY
SGEQSLKADIRGDDDPLDVCVLTEKNITHGNILLQARPIGGLRIIDSGEADDKIIAVLEDDLVFSEIKDISDCPCTVLDM
IQHYFLTYKATPEHLINAKPAKIEIVGIYGKKEAQKVIQLAHEDYLNKFVREKTTI

Sequences:

>Translated_216_residues
MSEKQSLSIMHPWHGPILTQDNYESLCCYIEITPQDSVKFELDKTTGLLKVDRPQKFSNFCPCLYGLLPRTYCGELSGQY
SGEQSLKADIRGDDDPLDVCVLTEKNITHGNILLQARPIGGLRIIDSGEADDKIIAVLEDDLVFSEIKDISDCPCTVLDM
IQHYFLTYKATPEHLINAKPAKIEIVGIYGKKEAQKVIQLAHEDYLNKFVREKTTI
>Mature_215_residues
SEKQSLSIMHPWHGPILTQDNYESLCCYIEITPQDSVKFELDKTTGLLKVDRPQKFSNFCPCLYGLLPRTYCGELSGQYS
GEQSLKADIRGDDDPLDVCVLTEKNITHGNILLQARPIGGLRIIDSGEADDKIIAVLEDDLVFSEIKDISDCPCTVLDMI
QHYFLTYKATPEHLINAKPAKIEIVGIYGKKEAQKVIQLAHEDYLNKFVREKTTI

Specific function: Unknown

COG id: COG0221

COG function: function code C; Inorganic pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790673, Length=182, Percent_Identity=31.3186813186813, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 5480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008162 [H]

Pfam domain/function: PF00719 Pyrophosphatase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 24366; Mature: 24235

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKQSLSIMHPWHGPILTQDNYESLCCYIEITPQDSVKFELDKTTGLLKVDRPQKFSNF
CCCCCCEEEECCCCCCEECCCCCCCEEEEEEECCCCCEEEEECCCCCEEEECCCCCHHHH
CPCLYGLLPRTYCGELSGQYSGEQSLKADIRGDDDPLDVCVLTEKNITHGNILLQARPIG
HHHHHHHCCHHHHHHCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCCCEEEEEEECCC
GLRIIDSGEADDKIIAVLEDDLVFSEIKDISDCPCTVLDMIQHYFLTYKATPEHLINAKP
CEEEEECCCCCCEEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHEEEECCHHHHCCCCC
AKIEIVGIYGKKEAQKVIQLAHEDYLNKFVREKTTI
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEKQSLSIMHPWHGPILTQDNYESLCCYIEITPQDSVKFELDKTTGLLKVDRPQKFSNF
CCCCCEEEECCCCCCEECCCCCCCEEEEEEECCCCCEEEEECCCCCEEEECCCCCHHHH
CPCLYGLLPRTYCGELSGQYSGEQSLKADIRGDDDPLDVCVLTEKNITHGNILLQARPIG
HHHHHHHCCHHHHHHCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCCCEEEEEEECCC
GLRIIDSGEADDKIIAVLEDDLVFSEIKDISDCPCTVLDMIQHYFLTYKATPEHLINAKP
CEEEEECCCCCCEEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHEEEECCHHHHCCCCC
AKIEIVGIYGKKEAQKVIQLAHEDYLNKFVREKTTI
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12682364 [H]