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The map label for this gene is priA [H]

Identifier: 62185415

GI number: 62185415

Start: 939626

End: 941875

Strand: Direct

Name: priA [H]

Synonym: CAB810

Alternate gene names: 62185415

Gene position: 939626-941875 (Clockwise)

Preceding gene: 62185414

Following gene: 62185419

Centisome position: 82.11

GC content: 40.49

Gene sequence:

>2250_bases
ATGGGTAATATTGAATCAACTACCTTTCGCCTATACGCAGAAGTCATAGTAAACTCCAATATCAATCAGGTATTAGACTA
CGGGCTCCCTCATCATCTCGAGCACATTACTAAGGGTACTGGTGTTAGCGTTTCCTTACGGGGAGCAAAAAAATATGGCA
TAGTCCATCAGATTAAAACACAAACAGAATGTAAACGAGTTCTTCCTGTTTTAGGTGTTATTGATTCTGGAATTATACTC
CCTCAAGATCTTCTAGAACTGATGTTTTGGATGAGTCAATATTATTTTGCGCCTTTGGGTAGGACGCTGCGTTTAGTGCT
TCCGGGAATATCTTCTAGTATCATACAGCCAAAACAGCAGTATCGCGTCCTACTCAAGCAAAGCAAAGCGAAGACTAAGG
AGATAATTCTTGCTATTCAAAAAGAATCTCCTGCGCAAGCAGCGACTTTAAAAACATTATTATCGTGTAGCTCTCCTCCT
GGTCTTTCCGAACTGATGGACAAGGCTAAGGTATCACAATCTCCGATCCATTCTTTAGAAAAACTGGGGGCTATTGAAAT
CGCCAGTGCTGCAGATCTTGAAATTCAAGAAGATCGTCTTACGTTCTTCCTTCCTGAAGCCCATGTGCTACATCCGCAAC
AACAAGATGCCGTGGATAAAATTTCTTCGTCCTTATCTTCAGGGAAATTTCAGACACATCTGATTTTTGGTGTTACAGGA
AGTGGAAAAACAGAAGTCTATTTCCAAGCTATTCGTGAGGCAAGGAAATTAGGGAAAAGTGCGATCCTTTTGGTTCCTGA
GATTGCTCTTACAATACAAACAGTGACCTTATTTAAAGCTCATTTTGGAAAAGAAGTGGGTATTCTCCATCACAAGCTGA
ATGACAGTGATAGAAATAAAACCTGGAGAGACGCGTCAAACGGAAATATTCATATCGTTATAGGTCCAAGATCGGCATTG
TTTTGTCCTTTACAGAATTTAGGACTGATCATCGTTGATGAAGAACACGATCCTGCCTATAAACAAAGTGAAAGCCATCC
CTGTTATCATGCTCGAGATGTTGCTGTTATGCGAGGGAAACTGGCAAATGCTACTGTAATATTAGGCAGTGCAACTCCTA
GTTTGGAGAGCTATGCCAATGCTTTATCAGGGAAGTATATTCTTTCAGAATTATCATCACGTGCTGCTTCTGCCTATCCT
GCGAAGATTTCTCTTATCGATATGAATATGGAAAGAGAAAAGACAAAGACAAAAACATTATTCTCTCAAGCTGCACTGAA
CAGTATTGAAAAACGGCTCGCTGTTGGAGAACAAGTCTTAGTTTTCTTTAATCGACGTGGCTACCATACAAACGTGTCTT
GTTCTTCGTGTAAACATACGTTGAAATGTGCACACTGCGATATGGTATTAACTTTCCATAAATATGCGAATGTTCTTCTC
TGTCATCTTTGTAATTCTTCCCCTAAAAACCCTCAGACATCGTGTCCAAAATGTCATGGCACCATGACTTTACAATATCG
TGGATCGGGGACAGAAAAAATAGAAAAAGTATTGCATAATATCTTCCCACAAGTGCGCACTATCCGTATTGATTCGGACA
CCACGAAATTTAAAGGAAGTCATGAATCTTTATTAAAGCAATTTGCCACAGGAAAAGCGGACATACTCATTGGAACACAA
ATGATAGCGAAAGGGATGCATTTTCCTGCTGTGACTTTAGCCATCATTTTAAATGGTGATTCTGGTTTGTACATTCCTGA
TTTCCGCGCTTCTGAACAAGTATTTCAATTGATCACACAAGTCACAGGAAGGTCGGGAAGGAGCCACCTCCCTGGGGAAG
TCTTGATTCAATCGTTTCTTCCTGATCACAGCACCATTCGCTGTGCTATCAGACAGGACTATCGAGCATTTTACCACCAA
GAAATCCCTGGGAGGGAATTGTGCAGCTACCCGCCATTTGTGCGTCTTGTTCGCTGTATATTCATAGGAAAATGCCCCAA
CCTCACGCGGAAAGAAGCGCAAAGAATCCACTCCATACTTAAAGAGAAGTTGGATACTCATACCCAATTAATGCAAATCA
CACCGTGTGGGCACTTCAAAATTAAAGATGTATTTCGGTATCAATTTTTAATAAAAAGCAAGCAAGTTTTGCCTGTAAAT
AAAAAACTCCATGAAGCTTTACTAACGGCAAAACTGTCTCCCAAAGTCAAATTCATGATTGACGTCGATCCGACAACCAC
ATTTTTCTAG

Upstream 100 bases:

>100_bases
ATGTACGTTATCATCAATCTTTCGTGAAAGATGGGTGCACTTGGATTCCTATTTATTCTTCAGTATACTATGAAAATGAT
CCGCAATTGAAACGAGATCT

Downstream 100 bases:

>100_bases
ATAGCTATGCAAAACAGCAATCAAGAGATCCACTTCATCTTGATGGTTAAACGCATGGAAATTGACTCGTATAAAAGGCT
TATCGGTAAAATTCACCCAG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 749; Mature: 748

Protein sequence:

>749_residues
MGNIESTTFRLYAEVIVNSNINQVLDYGLPHHLEHITKGTGVSVSLRGAKKYGIVHQIKTQTECKRVLPVLGVIDSGIIL
PQDLLELMFWMSQYYFAPLGRTLRLVLPGISSSIIQPKQQYRVLLKQSKAKTKEIILAIQKESPAQAATLKTLLSCSSPP
GLSELMDKAKVSQSPIHSLEKLGAIEIASAADLEIQEDRLTFFLPEAHVLHPQQQDAVDKISSSLSSGKFQTHLIFGVTG
SGKTEVYFQAIREARKLGKSAILLVPEIALTIQTVTLFKAHFGKEVGILHHKLNDSDRNKTWRDASNGNIHIVIGPRSAL
FCPLQNLGLIIVDEEHDPAYKQSESHPCYHARDVAVMRGKLANATVILGSATPSLESYANALSGKYILSELSSRAASAYP
AKISLIDMNMEREKTKTKTLFSQAALNSIEKRLAVGEQVLVFFNRRGYHTNVSCSSCKHTLKCAHCDMVLTFHKYANVLL
CHLCNSSPKNPQTSCPKCHGTMTLQYRGSGTEKIEKVLHNIFPQVRTIRIDSDTTKFKGSHESLLKQFATGKADILIGTQ
MIAKGMHFPAVTLAIILNGDSGLYIPDFRASEQVFQLITQVTGRSGRSHLPGEVLIQSFLPDHSTIRCAIRQDYRAFYHQ
EIPGRELCSYPPFVRLVRCIFIGKCPNLTRKEAQRIHSILKEKLDTHTQLMQITPCGHFKIKDVFRYQFLIKSKQVLPVN
KKLHEALLTAKLSPKVKFMIDVDPTTTFF

Sequences:

>Translated_749_residues
MGNIESTTFRLYAEVIVNSNINQVLDYGLPHHLEHITKGTGVSVSLRGAKKYGIVHQIKTQTECKRVLPVLGVIDSGIIL
PQDLLELMFWMSQYYFAPLGRTLRLVLPGISSSIIQPKQQYRVLLKQSKAKTKEIILAIQKESPAQAATLKTLLSCSSPP
GLSELMDKAKVSQSPIHSLEKLGAIEIASAADLEIQEDRLTFFLPEAHVLHPQQQDAVDKISSSLSSGKFQTHLIFGVTG
SGKTEVYFQAIREARKLGKSAILLVPEIALTIQTVTLFKAHFGKEVGILHHKLNDSDRNKTWRDASNGNIHIVIGPRSAL
FCPLQNLGLIIVDEEHDPAYKQSESHPCYHARDVAVMRGKLANATVILGSATPSLESYANALSGKYILSELSSRAASAYP
AKISLIDMNMEREKTKTKTLFSQAALNSIEKRLAVGEQVLVFFNRRGYHTNVSCSSCKHTLKCAHCDMVLTFHKYANVLL
CHLCNSSPKNPQTSCPKCHGTMTLQYRGSGTEKIEKVLHNIFPQVRTIRIDSDTTKFKGSHESLLKQFATGKADILIGTQ
MIAKGMHFPAVTLAIILNGDSGLYIPDFRASEQVFQLITQVTGRSGRSHLPGEVLIQSFLPDHSTIRCAIRQDYRAFYHQ
EIPGRELCSYPPFVRLVRCIFIGKCPNLTRKEAQRIHSILKEKLDTHTQLMQITPCGHFKIKDVFRYQFLIKSKQVLPVN
KKLHEALLTAKLSPKVKFMIDVDPTTTFF
>Mature_748_residues
GNIESTTFRLYAEVIVNSNINQVLDYGLPHHLEHITKGTGVSVSLRGAKKYGIVHQIKTQTECKRVLPVLGVIDSGIILP
QDLLELMFWMSQYYFAPLGRTLRLVLPGISSSIIQPKQQYRVLLKQSKAKTKEIILAIQKESPAQAATLKTLLSCSSPPG
LSELMDKAKVSQSPIHSLEKLGAIEIASAADLEIQEDRLTFFLPEAHVLHPQQQDAVDKISSSLSSGKFQTHLIFGVTGS
GKTEVYFQAIREARKLGKSAILLVPEIALTIQTVTLFKAHFGKEVGILHHKLNDSDRNKTWRDASNGNIHIVIGPRSALF
CPLQNLGLIIVDEEHDPAYKQSESHPCYHARDVAVMRGKLANATVILGSATPSLESYANALSGKYILSELSSRAASAYPA
KISLIDMNMEREKTKTKTLFSQAALNSIEKRLAVGEQVLVFFNRRGYHTNVSCSSCKHTLKCAHCDMVLTFHKYANVLLC
HLCNSSPKNPQTSCPKCHGTMTLQYRGSGTEKIEKVLHNIFPQVRTIRIDSDTTKFKGSHESLLKQFATGKADILIGTQM
IAKGMHFPAVTLAIILNGDSGLYIPDFRASEQVFQLITQVTGRSGRSHLPGEVLIQSFLPDHSTIRCAIRQDYRAFYHQE
IPGRELCSYPPFVRLVRCIFIGKCPNLTRKEAQRIHSILKEKLDTHTQLMQITPCGHFKIKDVFRYQFLIKSKQVLPVNK
KLHEALLTAKLSPKVKFMIDVDPTTTFF

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=753, Percent_Identity=33.9973439575033, Blast_Score=375, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259
- InterPro:   IPR006935 [H]

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII [H]

EC number: NA

Molecular weight: Translated: 83525; Mature: 83394

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNIESTTFRLYAEVIVNSNINQVLDYGLPHHLEHITKGTGVSVSLRGAKKYGIVHQIKT
CCCCCCHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHCCCCCEEEECCCHHHCHHHHHHH
QTECKRVLPVLGVIDSGIILPQDLLELMFWMSQYYFAPLGRTLRLVLPGISSSIIQPKQQ
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCHHH
YRVLLKQSKAKTKEIILAIQKESPAQAATLKTLLSCSSPPGLSELMDKAKVSQSPIHSLE
HHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHH
KLGAIEIASAADLEIQEDRLTFFLPEAHVLHPQQQDAVDKISSSLSSGKFQTHLIFGVTG
HCCCEEEECCCCCEEECCCEEEECCCHHCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECC
SGKTEVYFQAIREARKLGKSAILLVPEIALTIQTVTLFKAHFGKEVGILHHKLNDSDRNK
CCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHCCHHHHHEEECCCCCCCC
TWRDASNGNIHIVIGPRSALFCPLQNLGLIIVDEEHDPAYKQSESHPCYHARDVAVMRGK
CCCCCCCCEEEEEECCCCCEECCHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCC
LANATVILGSATPSLESYANALSGKYILSELSSRAASAYPAKISLIDMNMEREKTKTKTL
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHH
FSQAALNSIEKRLAVGEQVLVFFNRRGYHTNVSCSSCKHTLKCAHCDMVLTFHKYANVLL
HHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
CHLCNSSPKNPQTSCPKCHGTMTLQYRGSGTEKIEKVLHNIFPQVRTIRIDSDTTKFKGS
HHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCC
HESLLKQFATGKADILIGTQMIAKGMHFPAVTLAIILNGDSGLYIPDFRASEQVFQLITQ
HHHHHHHHHCCCCEEEEEHHHHHCCCCCCEEEEEEEEECCCCEECCCCCCHHHHHHHHHH
VTGRSGRSHLPGEVLIQSFLPDHSTIRCAIRQDYRAFYHQEIPGRELCSYPPFVRLVRCI
HHCCCCCCCCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHH
FIGKCPNLTRKEAQRIHSILKEKLDTHTQLMQITPCGHFKIKDVFRYQFLIKSKQVLPVN
HHCCCCCCCHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCHHHHHHHHHHHCCCCCCCCH
KKLHEALLTAKLSPKVKFMIDVDPTTTFF
HHHHHHHHHHCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
GNIESTTFRLYAEVIVNSNINQVLDYGLPHHLEHITKGTGVSVSLRGAKKYGIVHQIKT
CCCCCHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHCCCCCEEEECCCHHHCHHHHHHH
QTECKRVLPVLGVIDSGIILPQDLLELMFWMSQYYFAPLGRTLRLVLPGISSSIIQPKQQ
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCHHH
YRVLLKQSKAKTKEIILAIQKESPAQAATLKTLLSCSSPPGLSELMDKAKVSQSPIHSLE
HHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHH
KLGAIEIASAADLEIQEDRLTFFLPEAHVLHPQQQDAVDKISSSLSSGKFQTHLIFGVTG
HCCCEEEECCCCCEEECCCEEEECCCHHCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECC
SGKTEVYFQAIREARKLGKSAILLVPEIALTIQTVTLFKAHFGKEVGILHHKLNDSDRNK
CCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHCCHHHHHEEECCCCCCCC
TWRDASNGNIHIVIGPRSALFCPLQNLGLIIVDEEHDPAYKQSESHPCYHARDVAVMRGK
CCCCCCCCEEEEEECCCCCEECCHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCC
LANATVILGSATPSLESYANALSGKYILSELSSRAASAYPAKISLIDMNMEREKTKTKTL
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHH
FSQAALNSIEKRLAVGEQVLVFFNRRGYHTNVSCSSCKHTLKCAHCDMVLTFHKYANVLL
HHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
CHLCNSSPKNPQTSCPKCHGTMTLQYRGSGTEKIEKVLHNIFPQVRTIRIDSDTTKFKGS
HHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCC
HESLLKQFATGKADILIGTQMIAKGMHFPAVTLAIILNGDSGLYIPDFRASEQVFQLITQ
HHHHHHHHHCCCCEEEEEHHHHHCCCCCCEEEEEEEEECCCCEECCCCCCHHHHHHHHHH
VTGRSGRSHLPGEVLIQSFLPDHSTIRCAIRQDYRAFYHQEIPGRELCSYPPFVRLVRCI
HHCCCCCCCCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHH
FIGKCPNLTRKEAQRIHSILKEKLDTHTQLMQITPCGHFKIKDVFRYQFLIKSKQVLPVN
HHCCCCCCCHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCHHHHHHHHHHHCCCCCCCCH
KKLHEALLTAKLSPKVKFMIDVDPTTTFF
HHHHHHHHHHCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]