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The map label for this gene is pcnB [H]

Identifier: 62185370

GI number: 62185370

Start: 878219

End: 879505

Strand: Direct

Name: pcnB [H]

Synonym: CAB758

Alternate gene names: 62185370

Gene position: 878219-879505 (Clockwise)

Preceding gene: 62185365

Following gene: 62185371

Centisome position: 76.74

GC content: 37.45

Gene sequence:

>1287_bases
ATGGTCTGTGACAACAAAACTCTCTTGCGTAGAGGCTTAGAATTGTTTAGAAAAATTTCTAAATCTGCACCCACTCCAAT
TATCTACTCAGCTGCCGACCACAACATCAAACTTAAAAATTTTTCTCCACACGCACTTTCCGTAGTAAAGACCTTACGGA
AAGCTGGACATAAAGCGTATATTGTTGGCGGATGTATCCGCGATTTGCTACTCAACACAACTCCCAAAGATTTTGACATT
TCTACATCAGCTAAACCTGAGGAAATCAAAGCTGTTTTTAAGAATTGTATACTTGTTGGGAAACGCTTCCGCTTAGCGCA
TATTCGCTTCTCAAATCAAATCATTGAAGTCTCTACCTTCCGTTCGGGAAGTGCTGATGAGGATTGTCTCATTACCAAAG
ATAACCTTTGGGGGACTGCTGAGGAAGATGTTTTAAGGAGGGATTTTACCATCAATGGTTTATTCTATGATCCTACAGAA
GAAACGATCATAGATTATACTGGAGGTGTGAGCGATTTAGAGAACCGCTACTTAAGGACTATTGGCGATCCTTTTGTTCG
TTTTAAACAAGATCCCGTAAGAATGTTGCGTTTATTAAAAATACTCGCGAGAGCGCATTTCACTGTGGATCCTAAAACAT
TAGAAGCTCTTCAAGAGTGCCGTTATGAGTTAATTAAGAGTTCCCAAGCGCGTGTCTTTGAAGAACTCATAAAAATGCTA
GGTTCGGGAGTTTCCTCGCAGTTTTTTAAATTAACGGCAAAATATCAGATTTTAGAAATTCTTTTCCCTTATATGGACAA
AGCGTTTCGTCTCAATAAGACTCTAGAAGAACAAACCTTTGCCTGTTTGGATGTCTTAGATAGGCACATAGTCAACAAAA
AACATAACTATGACCGCCATCAGCTCATGGCGATATTTTTATTTCCTATTGTAAACTTTAATGTACGTTATAAGCACCGC
CAGTACCCGAGCCTTTCACTCACATCTGTCTTTGACTACATTAAAAATTTTTTAGGAAAGTTTTTTGCAGATTCATTTAC
CAGTTGTTCTAAAAAAAATTTTATTTTAACAGCTTTGATTTTGCAGATGCAATATCGGCTAACCCCATTAGTGCCAACGA
AAAAAATCCACTTCTTTAACCGGAAGTTTTTAAACCACGTACGTTTTTCTGAAGCTCTTTCCCTATTAGAAATACGTAGC
TTAGTTTATCCTAAGCTAGATAAAATACTTGCTGCTTGGATAAAGCATTACCAAGCATTACAATTTAAAAAGGAACTTCC
TTCTTGA

Upstream 100 bases:

>100_bases
ACTTCAATGTTTTTGTTATGGTGAAATTCCCTTAGAAAAAAGAGTGCTCATATAAACCCTTTTTCTTCCAAGACTTTTTA
ACACCTGATATCTATAATAA

Downstream 100 bases:

>100_bases
GTTTAAACTAGCCATGTTTCCTCTTTATCTGGTTCGTCTACTGTATCCTATAGGACTAATAGCTAATCTATTTTTTGGCT
TTGCGTTTACTCTCCAGTGG

Product: putative poly(A) polymerase

Products: NA

Alternate protein names: PAP [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MVCDNKTLLRRGLELFRKISKSAPTPIIYSAADHNIKLKNFSPHALSVVKTLRKAGHKAYIVGGCIRDLLLNTTPKDFDI
STSAKPEEIKAVFKNCILVGKRFRLAHIRFSNQIIEVSTFRSGSADEDCLITKDNLWGTAEEDVLRRDFTINGLFYDPTE
ETIIDYTGGVSDLENRYLRTIGDPFVRFKQDPVRMLRLLKILARAHFTVDPKTLEALQECRYELIKSSQARVFEELIKML
GSGVSSQFFKLTAKYQILEILFPYMDKAFRLNKTLEEQTFACLDVLDRHIVNKKHNYDRHQLMAIFLFPIVNFNVRYKHR
QYPSLSLTSVFDYIKNFLGKFFADSFTSCSKKNFILTALILQMQYRLTPLVPTKKIHFFNRKFLNHVRFSEALSLLEIRS
LVYPKLDKILAAWIKHYQALQFKKELPS

Sequences:

>Translated_428_residues
MVCDNKTLLRRGLELFRKISKSAPTPIIYSAADHNIKLKNFSPHALSVVKTLRKAGHKAYIVGGCIRDLLLNTTPKDFDI
STSAKPEEIKAVFKNCILVGKRFRLAHIRFSNQIIEVSTFRSGSADEDCLITKDNLWGTAEEDVLRRDFTINGLFYDPTE
ETIIDYTGGVSDLENRYLRTIGDPFVRFKQDPVRMLRLLKILARAHFTVDPKTLEALQECRYELIKSSQARVFEELIKML
GSGVSSQFFKLTAKYQILEILFPYMDKAFRLNKTLEEQTFACLDVLDRHIVNKKHNYDRHQLMAIFLFPIVNFNVRYKHR
QYPSLSLTSVFDYIKNFLGKFFADSFTSCSKKNFILTALILQMQYRLTPLVPTKKIHFFNRKFLNHVRFSEALSLLEIRS
LVYPKLDKILAAWIKHYQALQFKKELPS
>Mature_428_residues
MVCDNKTLLRRGLELFRKISKSAPTPIIYSAADHNIKLKNFSPHALSVVKTLRKAGHKAYIVGGCIRDLLLNTTPKDFDI
STSAKPEEIKAVFKNCILVGKRFRLAHIRFSNQIIEVSTFRSGSADEDCLITKDNLWGTAEEDVLRRDFTINGLFYDPTE
ETIIDYTGGVSDLENRYLRTIGDPFVRFKQDPVRMLRLLKILARAHFTVDPKTLEALQECRYELIKSSQARVFEELIKML
GSGVSSQFFKLTAKYQILEILFPYMDKAFRLNKTLEEQTFACLDVLDRHIVNKKHNYDRHQLMAIFLFPIVNFNVRYKHR
QYPSLSLTSVFDYIKNFLGKFFADSFTSCSKKNFILTALILQMQYRLTPLVPTKKIHFFNRKFLNHVRFSEALSLLEIRS
LVYPKLDKILAAWIKHYQALQFKKELPS

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=212, Percent_Identity=30.188679245283, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI87081691, Length=427, Percent_Identity=33.2552693208431, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1789436, Length=223, Percent_Identity=33.6322869955157, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71995920, Length=225, Percent_Identity=27.5555555555556, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321016, Length=229, Percent_Identity=28.82096069869, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281360112, Length=254, Percent_Identity=27.9527559055118, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21357337, Length=254, Percent_Identity=27.9527559055118, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 49732; Mature: 49732

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVCDNKTLLRRGLELFRKISKSAPTPIIYSAADHNIKLKNFSPHALSVVKTLRKAGHKAY
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHCCCEEE
IVGGCIRDLLLNTTPKDFDISTSAKPEEIKAVFKNCILVGKRFRLAHIRFSNQIIEVSTF
EHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEC
RSGSADEDCLITKDNLWGTAEEDVLRRDFTINGLFYDPTEETIIDYTGGVSDLENRYLRT
CCCCCCCCEEEECCCCCCCCHHHHHHHCCEECCEEECCCCCHHEECCCCHHHHHHHHHHH
IGDPFVRFKQDPVRMLRLLKILARAHFTVDPKTLEALQECRYELIKSSQARVFEELIKML
HCCHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
GSGVSSQFFKLTAKYQILEILFPYMDKAFRLNKTLEEQTFACLDVLDRHIVNKKHNYDRH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
QLMAIFLFPIVNFNVRYKHRQYPSLSLTSVFDYIKNFLGKFFADSFTSCSKKNFILTALI
HHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LQMQYRLTPLVPTKKIHFFNRKFLNHVRFSEALSLLEIRSLVYPKLDKILAAWIKHYQAL
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QFKKELPS
HHHHHCCC
>Mature Secondary Structure
MVCDNKTLLRRGLELFRKISKSAPTPIIYSAADHNIKLKNFSPHALSVVKTLRKAGHKAY
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHCCCEEE
IVGGCIRDLLLNTTPKDFDISTSAKPEEIKAVFKNCILVGKRFRLAHIRFSNQIIEVSTF
EHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEC
RSGSADEDCLITKDNLWGTAEEDVLRRDFTINGLFYDPTEETIIDYTGGVSDLENRYLRT
CCCCCCCCEEEECCCCCCCCHHHHHHHCCEECCEEECCCCCHHEECCCCHHHHHHHHHHH
IGDPFVRFKQDPVRMLRLLKILARAHFTVDPKTLEALQECRYELIKSSQARVFEELIKML
HCCHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
GSGVSSQFFKLTAKYQILEILFPYMDKAFRLNKTLEEQTFACLDVLDRHIVNKKHNYDRH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
QLMAIFLFPIVNFNVRYKHRQYPSLSLTSVFDYIKNFLGKFFADSFTSCSKKNFILTALI
HHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LQMQYRLTPLVPTKKIHFFNRKFLNHVRFSEALSLLEIRSLVYPKLDKILAAWIKHYQAL
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QFKKELPS
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]