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The map label for this gene is kdsB

Identifier: 62185185

GI number: 62185185

Start: 644927

End: 645691

Strand: Direct

Name: kdsB

Synonym: CAB569

Alternate gene names: 62185185

Gene position: 644927-645691 (Clockwise)

Preceding gene: 62185181

Following gene: 62185186

Centisome position: 56.36

GC content: 41.31

Gene sequence:

>765_bases
ATGGAAGAACAGACATTTATCAGTAAGAAGGTGGGAGTTTTACCAGCGAGATGGGGTAGTGTAAGATTTCCTGGGAAACC
CTTAGCCATGATCCTGGGAAAGTCCTTAATCCAAAGAACTTACGAAAATATCATCCAAAGCGAAACACTAGATAAAGTAG
TTGTAGCTACTGATGATCAACGCATTATGGATCACGTTCTAGATTTTGGTGGTGCGTGTCTGATGACGAGTCCAGAGTGC
GCTAATGGTACCGAACGTATGGCGGAAACCGTATCGCGTTATTTCCCTGAGGCTGAGATTATGGTGAACATCCAAGGAGA
TGAGCCCTGTTTACGCCATACTGTTGTCGATGCCCTTGTAAGAAAACTGGAAGAGCATCCAGAAATTCAAATGGTGACTC
CCGTGGCCCAAACCACAGATTCTCATGAAATCTTAACAAATCAAAAAGTAAAATGTGTTTTCGATAAAAATGGAAAGGCT
TTATATTTTAGCAGGAGCCCTATTCCACACATTTTAAAGAAAGAAACACCAATTTATCTACATATTGGCGTGTATGCATT
TAGAAGATCTGCTCTGTTCAGTTACATTGCTTCCGCTCCTTCTCCTCTAAGTCAAGCTGAAGATCTTGAGCAACTACGTG
TACTAGAACACGGTGGATCCATTCATGTATGTGTAGTGGAAGCTAAGAGCCCTTCGGTGGATTATCCAGAAGATATCAGT
AAGGTGGAAGAATACTTAACATGTCATTCAAGTGCATCTTTTTAA

Upstream 100 bases:

>100_bases
TACAAAAAATCAAATTCATCAAAAACCTTGTGTTTTATAAACCCTCCAATAATAATCCCAACTGTAAAAAATCTTAGACA
CTAAGGAGTTTCTGCGGCTT

Downstream 100 bases:

>100_bases
CAGGAGGAGTCGTATCCTCCTTAGGCAAGGGATTAACTGCTGCCTCACTTGCTCTACTACTAGAGCGACAAGGACTTAAA
GTTGCTATGTTAAAACTGGA

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MEEQTFISKKVGVLPARWGSVRFPGKPLAMILGKSLIQRTYENIIQSETLDKVVVATDDQRIMDHVLDFGGACLMTSPEC
ANGTERMAETVSRYFPEAEIMVNIQGDEPCLRHTVVDALVRKLEEHPEIQMVTPVAQTTDSHEILTNQKVKCVFDKNGKA
LYFSRSPIPHILKKETPIYLHIGVYAFRRSALFSYIASAPSPLSQAEDLEQLRVLEHGGSIHVCVVEAKSPSVDYPEDIS
KVEEYLTCHSSASF

Sequences:

>Translated_254_residues
MEEQTFISKKVGVLPARWGSVRFPGKPLAMILGKSLIQRTYENIIQSETLDKVVVATDDQRIMDHVLDFGGACLMTSPEC
ANGTERMAETVSRYFPEAEIMVNIQGDEPCLRHTVVDALVRKLEEHPEIQMVTPVAQTTDSHEILTNQKVKCVFDKNGKA
LYFSRSPIPHILKKETPIYLHIGVYAFRRSALFSYIASAPSPLSQAEDLEQLRVLEHGGSIHVCVVEAKSPSVDYPEDIS
KVEEYLTCHSSASF
>Mature_254_residues
MEEQTFISKKVGVLPARWGSVRFPGKPLAMILGKSLIQRTYENIIQSETLDKVVVATDDQRIMDHVLDFGGACLMTSPEC
ANGTERMAETVSRYFPEAEIMVNIQGDEPCLRHTVVDALVRKLEEHPEIQMVTPVAQTTDSHEILTNQKVKCVFDKNGKA
LYFSRSPIPHILKKETPIYLHIGVYAFRRSALFSYIASAPSPLSQAEDLEQLRVLEHGGSIHVCVVEAKSPSVDYPEDIS
KVEEYLTCHSSASF

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=242, Percent_Identity=37.1900826446281, Blast_Score=144, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB_CHLAB (Q5L5S2)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_219970.1
- HSSP:   P04951
- ProteinModelPortal:   Q5L5S2
- SMR:   Q5L5S2
- GeneID:   3337877
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB569
- HOGENOM:   HBG637773
- OMA:   IIPARLK
- PhylomeDB:   Q5L5S2
- ProtClustDB:   PRK05450
- BioCyc:   CABO218497:CAB569-MONOMER
- BRENDA:   2.7.7.38
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 28409; Mature: 28409

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEQTFISKKVGVLPARWGSVRFPGKPLAMILGKSLIQRTYENIIQSETLDKVVVATDDQ
CCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCH
RIMDHVLDFGGACLMTSPECANGTERMAETVSRYFPEAEIMVNIQGDEPCLRHTVVDALV
HHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHH
RKLEEHPEIQMVTPVAQTTDSHEILTNQKVKCVFDKNGKALYFSRSPIPHILKKETPIYL
HHHHHCCCEEEECCHHHCCCCHHHHCCCCEEEEEECCCCEEEEECCCCCHHHCCCCCEEE
HIGVYAFRRSALFSYIASAPSPLSQAEDLEQLRVLEHGGSIHVCVVEAKSPSVDYPEDIS
EHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHH
KVEEYLTCHSSASF
HHHHHHHHHCCCCC
>Mature Secondary Structure
MEEQTFISKKVGVLPARWGSVRFPGKPLAMILGKSLIQRTYENIIQSETLDKVVVATDDQ
CCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCH
RIMDHVLDFGGACLMTSPECANGTERMAETVSRYFPEAEIMVNIQGDEPCLRHTVVDALV
HHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHH
RKLEEHPEIQMVTPVAQTTDSHEILTNQKVKCVFDKNGKALYFSRSPIPHILKKETPIYL
HHHHHCCCEEEECCHHHCCCCHHHHCCCCEEEEEECCCCEEEEECCCCCHHHCCCCCEEE
HIGVYAFRRSALFSYIASAPSPLSQAEDLEQLRVLEHGGSIHVCVVEAKSPSVDYPEDIS
EHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHH
KVEEYLTCHSSASF
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA